• 제어로봇시스템학회:학술대회논문집
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• 1990.10b
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• pp.1156-1161
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• 1990

The concept of the manipulability measure of the robotic mechanism is extended to the dual arm holding a single object. This is a measure of manipulating ability of the dual arm forming a closed kinematic chain in positioning and orienting the object. Dual arm manipulability measure is defined and compared to the single arm manipulability measure, and some properties are investigated.

• The Plant Pathology Journal
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• v.22 no.2
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• pp.164-167
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• 2006

An immunocapture reverse transcription-polymerase chain reaction (IC/RT-PCR) was developed for simultaneous detection of two pepper-infecting RNA viruses, Pepper mud mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV). The assay could be performed in a single tube for simultaneous and sensitive detection of these tobamoviruses. This detection system revealed thousand-fold increase in detection sensitivity compare to ELISA. This method could save time and reagent cost compare to common RT-PCR which needs several reactions and several procedures of viral RNA extractions for the same number of samples.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3065-3069
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• 2016

Single nucleotide polymorphism (SNP) detection has been used extensively for genetic association studies of diseases including cancer. For mass, yet accurate and more economic SNP detection we have optimized tetra primer amplification refractory mutation system polymerase chain reaction (ARMS PCR) to detect three SNPs in the cytochrome P450 2E1 (CYP2E1) gene locus; i.e. rs3813865, rs2070672 and rs3813867. The optimization system strategies used were (1) designing inner and outer primers; (2) determining of their optimum primer concentration ratios; and (3) determining of the optimum PCR annealing temperature. The tetra primer ARMS PCR result could be directly observed using agarose gel electrophoresis. The method succesfully determined three SNPs in CYP2E1 locus, the results being consistent with validation using DNA sequencing and restriction fragment length polymorphisms (RFLP).

• Management Science and Financial Engineering
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• v.17 no.2
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• pp.1-21
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• 2011

This paper considers an integrated inventory-distribution system with a fleet of heterogeneous vehicles employed where a single warehouse distributes a single type of products to many spatially distributed retailers to satisfy their dynamic demands. The problem is to determine order planning at the warehouse, and also vehicle schedules and delivery quantities for the retailers with the objective of minimizing the sum of ordering cost at the warehouse, inventory holding cost at both the warehouse and retailers, and transportation cost. For the problem, we give a Mixed Integer Programming formulation and develop a Lagrangean heuristic procedure for computing lower and upper bounds on the optimal solution value. The Lagrangean dual problem of finding the best Lagrangrean lower bound is solved by subgradient optimization. Computational experiments on randomly generated test problems showed that the suggested algorithm gives relatively good solutions in a reasonable amount of computation time.

• Animal cells and systems
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• v.2 no.3
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• pp.371-375
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• 1998

Twenty-one monoclonal anti-DNA autoantilndies were produced by fusing spleen cells from an autoimmune MRL/lpr mouse with SP2/0 myeloma cells. Hybridomas generated by the fusions were chosen for cloning on the basis of DNA binding by supernatant antibody. Each monoclonal antibody was purified to homogeneity and analyzed for the heavy and light chain isotypes and the binding specificity for single-stranded DNA, double-stranded DNA, and RNA. Sequence specificities and isoelectric points of the antibodies were also examined. All of the antibodies were lgG and tended to bind to both single-stranded and double-stranded DNA with a preference for the double-stranded form. Some of them also bound to RNA. Isoelectric points of the antibodies were shown to be high. The antibodies described in this report have characteristics of pathogenic anti-DNA antibodies.

• Molecules and Cells
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• v.19 no.3
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• pp.452-457
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• 2005

The adaptor protein (AP) complexes are involved in membrane transport of many proteins. There are 3 AP complexes in C. elegans unlike mammals that have four. To study the biological functions of the AP-3 complexes of C. elegans, we sought homologues of the mouse and human genes that encode subunits of the AP-3 complexes by screening C. elegans genomic and EST sequences. We identified single copies of homologues of the ${\mu}3$, ${\sigma}3$, ${\beta}3$ and ${\delta}$ genes. The medium chain of AP-3 is encoded by a single gene in C. elegans but two different genes in mammals. Since there are no known mutations in these genes in C. elegans, we performed RNAi to assess their functions in development. RNAi of each of the genes caused embryonic and larval lethal phenotypes. APM-3 is expressed in most cells, particularly strongly in spermatheca and vulva. We conclude that the products of the C. elegans ${\mu}3$, ${\sigma}3$, ${\beta}3$ and d genes are essential for embryogenesis and larval development.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.53-54
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• 2006

In this paper, we analytically evaluate packet delay and energy consumption of S-MAC protocol with a modified Markov chain model. Although some models, based on IEEE 802.11 MAC protocol, to analyze the S-MAC protocol in wireless sensor network (WSN) have been proposed, they fail to consider the differences in architecture between the S-MAC and the 802.11 MAC. Therefore, by reflecting the significant features in the S-MAC function, we model the operation of S-MAC protocol, and derive its packet delay and energy consumption in single-hop WSN. Numerical results show the delay and the dissipated energy at various duty cycle values according to offered load, where a practical mote is used.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.31 no.1
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• pp.49-58
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• 2008

In this paper, we consider an inventory system where a single supplier purchases and processes raw materials into finished goods in order to deliver finished goods to a single buyer for effective implementation of Just-In-Time Purchasing. An integrated multi-item lot-splitting model of facilitating multiple shipments in small lots between buyer and supplier is developed in a JIT Purchasing environment. Also, an iterative solution procedure is developed to find the order quantity for finished goods and raw materials, and number of shipments between buyer and supplier. We show by numerical example that when the integrated policy is adopted by both buyer and supplier in a cooperative manner, both parties can benefit.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.375-377
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• 2003

In this study, we employ the control patches, which have been created in previous photogrammetric projects, serving for the candidates of the control points that are likely to be found in the newly taken photos by utilizing image matching technique. Among others, the successful implementation of the above idea lies in the underlying factors: (1). Predicting the control patches and projecting them onto new photos; (2). Alignment of control patches with respect to the new photos; (3). Generating the equivalent ground elements of control patches versus the new photos for the purpose of correlation; (4). Developing effective matching methods and matching strategy; (5). Refining the exterior orientation parameters. What may show significance in this work comparing to traditional aerial-triangulation chain is that whenever at least three matched control patches succeed in a single photo, it follows that single photo orientation is applicable. The experiments suggest the potential efficiency of automatic control point measurement from control patch database and photo orientation by the proposed workflow.

• Journal of Genetic Medicine
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• v.3 no.1
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• pp.15-19
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• 1999

We have investigated the genetic variation in the human apo B mRNA editing protein (apobec-1) gene. Exon 3 of the apobec-1 gene was amplified by polymerase chain reaction. After detection of an additional band by single strand conformational polymorphism (SSCP) analysis, sequencing of the SSCP-shift sample revealed a single-base mutation. The mutation was a CGG transversion at codon 80 resulting in a lleRMet substitution. This substitution was confirmed by restriction fragment length polymorphism analysis since a Pvull site is abolished by the substitution. Population and family studies confirmed that the inheritance of the genotypes for apobec-1 gene polymorphism is controlled by two codominant alleles (P1 and P2). A significant difference in plasma triglyceride was detected among the different apobec-1 genotypes in the CAD patients (P<0.05). Our study could provide the basis for elucidating the interaction between genetic variation of the apobec-1 gene and disorders related to lipid metabolism.