• Title/Summary/Keyword: simultaneous removal

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Changes of Microbial Community Depending on Different Dissolved Oxygen in Biological Nitrogen Removal Process (생물학적 질소제거 공정에서 용존산소변화에 따른 미생물의 군집변화)

  • Park, Jong-Il;Lee, Tae-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.9
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    • pp.939-947
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    • 2008
  • PCR-DGGE method was applied to analyze changes of microbial community in simultaneous nitrification and denitrification (SND) bioreactor with various DO concentrations. In the analysis of eubacterial community, band profiles of DGGE were similar with 2 or 1 mg/L DO concentrations in the reactor. Experimental results led to 16 different bacteria being identified, including 5 dominant strains(3 strains of Uncultured Bacterium, 1 strains of Bacillus, 1 strains of Uncultured Bacteroidetes). DGGE results at 0.5 mg/L DO concentration led to 12 strains being identified, including 7 dominant strains(5 strains of Uncultured Bacterium, 2 strains of Zoogloea sp.). DGGE results at 0.1 mg/L DO concentration led to 11 strains being identified, including 3 dominant strains(1 strains of Uncultured Bacterium, 2 strains of Zoogloea sp.). In DGGE band profiles of $\beta$-AOB($\beta$-Ammonia Oxidizing Bacteria), only one band was observed. This band had 97% similarity with Nitrosomonas sp. done DNB Y20. This band was clearly observed at the 2, 1 and 0.5 mg/L DO concentrations, while the brightness of the band at 0.1 mg/L DO concentration was mostly dimmed. In DGGE band profiles of denitrification process, 5 bands(3 strains of Uncultured organism containing nirS, 2 strains of Uncultured organism containing nirK) were observed. Among those bands, the brightness of one band was gradually increased at the lower DO concentrations. This band has 86% identity with Uncultured organism clone eS1 cd1 nirS gene, partial cds. Based on this result, it could be concluded that Uncultured organism clone eS1 cd1 nirS gene, partial cds is a predominant microorganism in the denitrification process.

Transient Behaviors of a Two-Stage Biofilter Packed with Immobilized Microorganisms when Treating a Mixture of Odorous Compounds (미생물 포괄고정화 담체를 이용한 이단 바이오필터에서의 오염부하량 동적 부하변동시 복합악취 제거효율 변화특성)

  • NamGung, Hyeong-Kyu;Shin, Seung-Kyu;Hwang, Sun-Jin;Song, Ji-Hyeon
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.12
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    • pp.1126-1133
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    • 2010
  • A two-stage biofilter was constructed and utilized to determine the removal efficiency when treating dynamic loading of a mixture of odorous compounds including benzene, toluene, p-xylene, ammonia and hydrogen sulfide. A yeast strain, Candida tropicalis, and a sulfur oxidizing bacterial (SOB) strain, Acidithiobacillus caldus sp., were immobilized in polyurethane media and packed in the two-stage biofilter. The experiment of dynamic loading variation was composed of (1) stepwise loading variation of all the odorous compounds (total EC test), (2) stepwise loading variation of each odorous compound, and (3) intermittent loading variation with 2-day-off and 3-day-on. The total EC test showed that the maximum elimination capacity was $61\;g/m^3/hr$ for total VOCs, and 5.2 and $9.1\;g/m^3/hr$ for ammonia and hydrogen, respectively. In addition, the inhibition between VOCs was observed when the loading of each individual VOC was varied. Especially the stepwise increase in toluene loading resulted in decreases of benzene and p-xylene removal efficiencies about 30% and 25%, respectively. However, the inhibition between organic and inorganic compounds was not observed. The intermittent loading variation with 2-day-off and 3-day-on showed that greater than 95% of the overall removal efficiency was restored in two days after the loading resumed. Consequently, the two-stage biofilter packed with immobilized microorganisms showed advantages over conventional biofilters for the simultaneous treatment of the mixture of organic and inorganic odorous compounds.

Effect of Added NH$_3$ to AMP on Absorption Rate for Simultaneous Removal of CO$_2$/NO$_2$ in Composite Absorption Process (복합흡수공정에서 CO$_2$/NO$_2$ 동시제거 시 AMP(2-amino-2-methyl-1-propanol)에 Ammonia 첨가가 흡수속도에 미치는 영향)

  • Seo, Jong-Beom;Choi, Won-Joon;Moon, Seung-Jae;Lee, Gou-Hong;Oh, Kwang-Joong
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.12
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    • pp.1287-1293
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    • 2008
  • In this study, a blend of 2-amino-2-methyl-1-propanol (AMP) and ammonia (NH$_3$) was used to achieve high absorption rates for carbon dioxide (CO$_2$) as suggested at several literatures. The absorption rates of aqueous AMP and blended AMP+NH$_3$ solutions with CO$_2$ and nitrogen dioxide (NO$_2$) were measured using a stirred-cell reactor at 303 K. The effect of the added NH$_3$ to enhance absorption characteristics of AMP was studied. The performances were evaluated under various operating conditions. The absorption rates increased following the increase of the concentration of NH$_3$. The absorption rate of NH$_3$ blended into 30 wt.% AMP solution with NO$_2$ at 303 K was 12.6$\sim$32.6% higher than that of aqueous AMP solution without NH3. Also, the addition of 3 wt.% NH$_3$ to 30 wt.% AMP increased 48.2$\sim$41.6% values for the reactions with CO$_2$ and NO$_2$ at 303 K. Therefore, it clearly shows that the reaction rate of AMP with CO$_2$ and NO$_2$ can be increased by the addition of NH$_3$.

Removal of Mixed Odor(H$_2$S/CH$_3$SH) using Char Adsorbent Made from Sewage Sludge (하수슬러지 탄화물 흡착제를 이용한 혼합 악취(H$_2$S/CH$_3$SH)의 제거)

  • Han, Young-Suk;Choi, Won-Joon;Kim, Taek-Joon;Kim, Im-Gyung;Oh, Kwang-Joong
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.11
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    • pp.1132-1138
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    • 2008
  • The study was carried out to investigate adsorption characteristic on hydrogen sulfide (H$_2$S) and methylmercaptan (CH$_3$SH) odor gas using the char made by a thermal decomposition of sewage sludge. The fixed bed adsorption experiments of the optimum L/D ratio could be 1.0, and adsorption capacity and break point increased with the increase of temperature. A simultaneous adsorption characteristic of H$_2$S and CH$_3$SH increased in breakthrough time and adsorption capacity more than single adsorption experiment, and CH$_3$SH had higher effective diffusivities than H$_2$S in same condition. The adsorption capacity of CH$_3$SH increased with fast velocity. When it was compared the produced absorbent with commercial activated carbon, As to adsorbent amount, it was H$_2$S 77% and CH$_3$SH 80% of commercial activated carbon.

Screening of White Rot Fungi with Selective Delignification Capacity for Biopulping (백색목재부후균중 Biopulping에 이용가능한 선택적 리그닌분해균의 스크리닝)

  • Lee, Jong-Kyu;Oh, Eun-Sung
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.144-152
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    • 1998
  • To obtain white rot fungi which have selective delignification capacity and can be used in biopulping processes, 94 different wood rotting fungi were screened and the capabilities of selected species were evaluated on deciduous and coniferous wood blocks. White rot fungi, first of all, were selected by simple enzyme tests, i.e., cellulase activity test; phenol oxidase activity test; laccase and peroxidase activity test. Most organisms that gave a positive Bavendamm gave a strongly positive laccase test with syringaldazine whereas most of those that gave a negative Bavendamm test also negative test for laccase and peroxidase, even if some exceptions were noted. Wood decay experiement were carried out to select fungal species with selective lignin-degrading ability by inoculating selected fungi to both wood blocks of Populus tomentiglandulosa and Larix leptolepis. After 12 weeks of incubation, weight losses, lignin losses, and morphological characteristics of the decayed wood were investigated. Almost all fungi tested caused 2 or more times of weight losses in P. tomentiglandulosa than in L. leptolepis, while no weight losses were detected from the un-inoculated wood blocks. Ceriporiopsis subvermispora and Phanerochaete chrysosporium were the best delignifiers for both hardwood and softwood. P. chrysosporium, however, was less effective than C. subvermispora. Bjerkandera adusta and two unidentified spp. caused delignification for only P. tomentiglandulosa. B. adusta caused simultaneous rot of all cell wall components, resulted in thinning of the secondary cell wall layers. Other fungi caused selective delignification resulting in the removal of lignin from middle lamella and separation of cells from each other.

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The Efficacy of Bioabsorbable Mesh in Craniofacial Trauma Surgery

  • Choi, Won Chul;Choi, Hyun Gon;Kim, Jee Nam;Lee, Myung Cheol;Shin, Dong Hyeok;Kim, Soon Heum;Kim, Cheol Keun;Jo, Dong In
    • Archives of Craniofacial Surgery
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    • v.17 no.3
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    • pp.135-139
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    • 2016
  • Background: The ultimate goal of craniofacial reconstructive surgery is to achieve the most complete restoration of facial functions. A bioabsorbable fixation system which does not need secondary operation for implant removal has been developed in the last decade. The purpose of this study is to share the experience of authors and to demonstrate the efficacy of bioabsorbable mesh in a variety of craniofacial trauma operations. Methods: Between October 2008 and February 2015, bioabsorbable meshes were used to reconstruct various types of craniofacial bone fractures in 611 patients. Any displaced bone fragments were detached from the fracture site and fixed to the mesh. The resulting bone-mesh complex was designed and molded into an appropriate shape by the immersion in warm saline. The mesh was molded once again under simultaneous warm saline irrigation and suction. Results: In all patients, contour deformities were restored completely, and bone segments were fixed properly. The authors found that the bioabsorbable mesh provided rigid fixation without any evidence of integrity loss on postoperative computed tomography scans. Conclusion: Because bioabsorbable meshes are more flexible than bioabsorbable plates, they can be molded and could easily reconstruct the facial bone in three dimensions. Additionally, it is easy to attach bone fragments to the mesh. Bioabsorbable mesh and screws is effective and can be easily applied for fixation in various craniofacial trauma reconstructive scenarios.

The Simultaneous Nitrification and Organics Oxidation of Wastewater in Airlift Biofilm Reactors (공기리프트 생물막 반응기에서의 폐수 질화 및 유기물 동시산화)

  • 서일순;허충희
    • KSBB Journal
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    • v.16 no.6
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    • pp.573-578
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    • 2001
  • The effects of organic supplement (acetate) and dissolved oxygen concentration on the nitrification rate of wastewater were investigated in the 27.7 L pilot-scale airlift biofilm reactor with the granular activated carbon media of 0.613 mm diameter. The ammonium oxidation rate increased stepwise up to 5 kg N/㎥$.$d at the riser air velocity of 0.063 m/s, when the air velocity and the ammonium loading rate were raised alternately. The nitrite build-up was observed during the early stage of the biofilm formation, which disappeared after the reactor operation of 128 days. As increasing the organic loading rate, the organic oxidation rate increased up to 25.0 kg COD/㎥$.$d with the removal efficiency of 94% but the oxidation rates of ammonium and nitrite decreased. The oxidation rates of ammonium and nitrite increased with the dissolved oxygen concentrations. When the pure oxygen was sparged, the ammonium oxidation rate was almost five times higher than that with air at the same velocity.

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A new protocol of the sliding mechanics with Micro-Implant Anchorage(M.I.A.) (Micro-Implant Anchorage(MIA)를 이용한 Sliding mechancis)

  • Park, Hyo-Sang
    • The korean journal of orthodontics
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    • v.30 no.6 s.83
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    • pp.677-685
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    • 2000
  • Anchorage plays an important role in orthodontic treatment. Because of limited anchorage Potential and acceptance problems of intra- or extraoral anchorage aids, endosseous implants have been suggested and used. However, clinicians have hesitated to use endosseous implants as orthodontic anchorage because of limited implantation space, high cost, and long waiting period for osseointegration. Titanium miniscrews and microscrews were introduced as orthodontic anchorage due to their many advantages such as ease of insertion and removal, low cost, immediate loading, and their ability to be placed in any area of the alveolar bone. In this study, a skeletal Class II Patient was treated with sliding mechanics using M.I.A.(micro-implant anchorage). The maxillary micro-implants provide anchorage for retraction of the upper anterior teeth. The mandibular micro-implants induced uprighting and intrusion of the lower molars. The upward and forward movement of the chin followed. This resulted in an increase of the SNB angle, and a decrease of the ANB angle. The micro-implants remained firm and stable throughout treatment. This new approach to the treatment of skeletal Class II malocclusion has the following characteristics . Independent of Patient cooperation. . Shorter treatment time due to the simultaneous retraction of the six anterior teeth . Early change of facial Profile motivating greater cooperation from patients These results indicate that the M.I.A. can be used as anchorage for orthodontic treatment. The use of M.I.A. with sliding mechanics in the treatment of skeletal Class II malocclusion increases the treatment simplicity and efficiency.

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Optimization of solid phase extraction and simultaneous determination of trace anions in concentrated hydrofluoric acid by ion chromatography (불산 중 극미량 음이온 분석을 위한 고상 추출법 및 이온크로마토그래프를 이용한 동시분석법 확립)

  • Yoon, Suk-Hwan;Jo, Dong-ho;Kim, Hyun-Ji;Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.29 no.5
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    • pp.219-224
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    • 2016
  • 불산 중 극미량 음이온의 고상추출과 이온크로마토그래프를 이용한 고감도 분석법이 개발되었다. 불산 중 불소이온이 고상에 의해 제거하였고 이어서 음이온 (F, CH3COO, Cl, Br, NO3, PO43−, SO42−)들이 이온크로마토그래프를 이용하여 연속적으로 분리하였다. 고상 추출법에 영향을 주는 각 인자들 (흡착제의 선택, 시료의 부피 및 pH, 용출 용액과 용출용액의 부피)을 결정하였으며 그 결과 흡착제로서 Oasis WAX 컬럼이 가장 우수하였고 1.0 mL의 시료부피, 용출용액으로 50 mM 초산암모늄염 5 mL가 분리능에서 가장 우수하였다. 개발한 방법에 의한 음이온 (Cl, Br, NO3, PO43−, SO42−)들의 방법검출한계는 25 % 불산용액 (w/w) 중에 0.04~0.30 µg/L의 범위를 보였고 정밀도는 20.0와 40.0 µg/L의 농도에서 5 % 이내를 보였다. 한 제조회사에 의한 25 % 불산 중 음이온의 4.2에서 47.5 µg/L의 범위로 모두 검출되었다. 이 방법은 시험절차가 간단하고, 재현성 및 감도가 좋아서 반도체회사에서 불산 중 음이온 불순물을 정도 관리하는데 매우 유용한 방법이 될 것으로 판단된다.

Comparison of Anoxic/Oxic Membrane Bioreactor - Reverse Osmosis and Activated Sludge Process-Microfiltration-Reverse Osmosis Process for Advanced Treatment of Wastewater (폐수의 고도처리를 위한 무산소/호기형 분리막생물반응조 - 역삼투 공정과 활성슬러지공정 - 정밀여과 - 역삼투 공정의 비교)

  • Roh, Sung-Hee;Kim, Sun-Il;Quan, Hong-hua;Song, Yon-Ho
    • Applied Chemistry for Engineering
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    • v.17 no.5
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    • pp.521-526
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    • 2006
  • A membrane bioreactor (MBR) is an effective tool for wastewater treatment with recycling. MBR process has several advantages over conventional activated sludge process (ASP); reliability, compactness, and quality of treated water. The resulting high-quality and disinfected effluents suggest that MBR process can be suitable for the reused and recycling of wastewater. An anoxic/oxic (A/O) type MBR was applied to simultaneous removal of organics and nutrients in sewage. At first, the efficiency of submerged MBR process was investigated using a hollow fiber microfiltration membrane with a constant flux of $10.2L/m^2{\cdot}h$ at each solids retention time (SRT). Results showed that protein/carbohydrate (P/C) ratio increased and total extracellular polymeric substances (EPS) remained constant with SRT increased. Secondly, A/O type MBR with a reverse osmosis (RO) membrane was employed to treat the municipal wastewater. The performance of A/O type MBR-RO process is better for the treatment of organics and nutrients than ASP-MF-RO process in terms of consistent effluents quality.