• Molecules and Cells
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• v.27 no.4
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• pp.391-396
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• 2009

Image-based, high-content screening assays demand solutions for image segmentation and cellular compartment encoding to track critical events - for example those reported by GFP fusions within mitosis, signalling pathways and protein translocations. To meet this need, a series of nuclear/cytoplasmic discriminating probes have been developed: DRAQ5$^{TM}$ and CyTRAK Orange$^{TM}$. These are spectrally compatible with GFP reporters offering new solutions in imaging and cytometry. At their most fundamental they provide a convenient fluorescent emission signature which is spectrally separated from the commonly used reporter proteins (e.g. eGFP, YFP, mRFP) and fluorescent tags such as Alexafluor 488, fluorescein and Cy2. Additionally, they do not excite in the UV and thus avoid the complications of compound UV-autofluorescence in drug discovery whilst limiting the impact of background sample autofluorescence. They provide a convenient means of stoichiometrically labelling cell nuclei in live cells without the aid of DMSO and can equally be used for fixed cells. Further developments have permitted the simultaneous and differential labelling of both nuclear and cytoplasmic compartments in live and fixed cells to clearly render the precise location of cell boundaries which may be beneficial for quantitative expression measurements, cell-cell interactions and most recently compound in vitro toxicology testing.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.411-413
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• 2012

This study has been investigated about hygrothermal environmental effects on the pin-joined carbon/epoxy composites through acoustic emission technique. The specimens were classified as three types for pin loading test : Base (before immersion), RT (room temperature immersion), HT ($75^{\circ}C$ immersion). As a results, the bearing strength of RT specimens was weakly decreased than that of base specimens. The bearing strength of HT specimens was greatly decreased than that of Base and RT specimens due to effects of simultaneous moisture and higher temperature. Also, the results from cumulative hit of acoustic emission indicated that AE events induced by matrix cracks of HT specimens was lower than that of base specimens.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.473-478
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• 2016

With the increasing development and commercial use of genetically modified maize, it is essential to develop an appropriate method for detection of individual LMO (Living modified organism) events for monitoring the samples. In South Korea, commercial planting and accidental or unintentional releases of LMOs into the environment were not approved. In this study, to increase the efficiency of LMO detection, we developed simultaneous detection methods for 11 LM maize events. This multiplex PCR detection method is economical, as it saves time, cost and labor. We developed 11 individual LM maize events, and applied 4 multiplex PCR sets to the LM maize samples. These results are confirmed by applying the multiplex analysis of LMO environmental monitoring from 2012 to 2014, which represents the unintentionally released LM maize samples. The data were correlated with event specific PCR results. Our results indicate that the multiplex PCR method developed is suitable for detection of LM maize in LMO monitoring.

• Journal of Chest Surgery
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• v.16 no.2
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• pp.199-212
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• 1983

The inhibition/influences of adenine compounds on the heart have been described repeatedly by many investigators, since the first report by Druny and Szent-Gyorgyi [1929]. These studies have shown that adenosine and adenine nucleotides have an over-all effect similar to that of acetylcholine [ACh] by slowing and weakening the heartbeat. The basic cellular and membrane events underlying the inhibitory action of adenosine on sinus rate, however, are not well understood. Furthermore, the physiological role of adenosine in regulation of the heartbeat remains still to be elucidated. Therefore, this study was undertaken in order to examine the response of rabbit SA node to adenosine and to compare the response to that of ACh. Isolated SA node preparation, whole atrial pair, or left atrlal strip was used in each experiment. Action potentials of SA node were recorded through the intracellular glass microelectrodes, which were filled with 3M KCI and had resistance of 30-50 M. All experiments were performed in a bicarbonate-buffered Tyrode solution which was aerated with 3% $CO_2-97%$ $O_2$ gas mixture and kept at $35^{\circ}C$. Spontaneous firing rate of SA node at 35C [Mean + SEM, n=16] was 154 + 3.3 beats/min. The parameters of action potentials were: maximum astolic potential [MDP], -731.7mV: overshoot [OS], 9 + 1.4mV; slope of pacemaker potential [SPP], 94 3.0mV/sec.Adenosine suppressed the firing rate of SA node in a dose dependent manner. This inhibitory effect appeared at the concentration of $10^{-6}M$ and was potentiated in parallel with the increase in adenosine concentration. Changes in action potential by adenosine were dose-dependent increase of MDP and decrease of SPP until $10^{-4}$. Above this concentration, however, the amplitude of action potential decreased markedly due to the simultaneous decrease of both MDP and OS. All these effects of adenosine were not affected by pretreatment of atropine [2mg/l] and propranolol [$5{\times}10^{-6}M$]. ACh [$10^{-6}M$] responses on action potential were similar to those of adenosine by increasing MDP and decreasing SPP. These effects of ACh disappeared by pretreatment of atropine [2mg/1]. Inhibition/effects of adenosine and ACh on sinus rate were enhanced synergistically with the simultaneous administration of adenosine and ACh. Marked decrease of overshoot potential was the most prominent feature on action potential. Dipyridamole [DPM], which is known to block the adenosine transport across cell membrane, definitely potentiated the action of adenosine . Adenosine suppressed the sinus rate and atrial contractility in the same dosage range, even in the reserpinized preparation. Above` results suggest that adenosine suppresses pacemaker activity, like ACh, by acting directly on the membrane of SA node, increasing MDP and decreasing SPP.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.117-122
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• 2015

Canola is a crop globally used for production of oil and biofuel. Cultivation area and import volume of living modified (LM) canola have been increasing every year. As canola import dependence has reached 100% in Korea, efforts have been made for safety management of LM canola and ecological risk assessment. We developed a set of multiplex PCR method for simultaneous detection of 5 LM canola events (Topas 19/2, Rf3, Ms8, RT73 and T45) approved in Korea. The multiplex PCR assay developed allows amplification of estimated products of 5 LM canolas from event specific primer sets. Primer extension time was skipped for a time-consuming process and two annealing steps (20 cycles at $55^{\circ}C$ and 20 cycles at $60^{\circ}C$) were performed for yielding the best result which was sufficient to distinguish five LM canolas. Our results suggest that multiplex PCR method provides a cost and time-effective approach for LM canola detection.

• Knowledge Management Research
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• v.18 no.1
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• pp.83-116
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• 2017

The purpose of this study is to analyze empirically analyze how the characteristics of participants in educational and social learning forums and the quality of events influence expectations and satisfaction of forums. The study also aims to provide strategic implications for forum organizers and give them suggestions on how to set up target audience, manage forum contents, speakers, and services, improve attendee satisfaction, and ultimately maximize overall outcomes. As exchanges among individuals, enterprises, and organizations, as well as countries are growing rapidly, the convention industry has become a key player in the market. Conventions have also become a venue for people to discuss a specific agenda or topic, exchange information and learn knowledge and insights. Especially, the forum - as part of the convention industry - plays a vital role providing educational and social learning opportunities as scholars and expertise come together to share their knowledge and experience through a variety of discussions. With its role, many of forums are taking place in recent years; however, there have been few empirical studies upon the forum itself. Also, there have been few attempts to research how the quality of forums affect participants' satisfaction along with their characteristics and how much of practical knowledge is provided throughout the events. This study is meaningful in that it is the first practical study that takes a deep understanding of the forum and sees how the quality of the forums influences participants' satisfaction and whether the characteristics of participants have a moderating effect in increasing the level of satisfaction. Forum organizers could also take a strategic approach as their major concerns are to increase the number of participants and raise degree of satisfaction by providing significant information. There are four key elements that determine success or failure of a social learning forum. The four elements are contents, speakers, services, and participants. Content plays an important role in providing rich information and knowledge for participants. Speakers are the main knowledge providers who contribute to the forum's social learning role. Also, the services provided by forum organizers such as simultaneous interpretation services, program brochures, lunch and refreshments, and the overall design of event hall can also influence the level of participants' satisfaction. Lastly, the participants and their characteristics are important since they are the ones who receive knowledge from the providers. The results of this study show that the quality of forum (content, speaker, and services) has a decisive effect on the participants' satisfaction and there are some differences in expectation among the participants in the forum. Also, some groups of participants were more likely to be stimulated by the quality of forum when determining their satisfaction. The study is modeled after MBN Y Forum 2016 and its participants' characteristics. The forum is one of the most representative social learning forums of South Korea and its audiences are mostly young people. It has analyzed how the participants' characteristics influence their satisfaction by grouping them into ${\Delta}participants$ who have invited for free and those who paid for the entrance fee, ${\Delta}first-time$ participants and returning participants, ${\Delta}voluntary$ and involuntary participants, ${\Delta}participants$ who registered through web and those who did through mobile, and ${\Delta}participants$ who registered during pre-sale opens and those who registered during general opens.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.63.3-64
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• 2015

In our previous work (Kim et al., 2015), we suggested a new classification scheme, which categorizes the SPEs into four groups based on association with flare or CME inferred from onset timings as well as proton acceleration patterns using multienergy observations. In this study, we have tried to find whether there are any typical characteristics of associated events and acceleration sites in each group using 42 SPEs from 1997 to 2012. We find: (i) if the proton acceleration starts from a lower energy, a SPE has a higher chance to be a strong event (>5000pfu) even if the associated flare and CME are not so strong. The only difference between the SPEs associated with flare and CME is the location of the acceleration site. For the former, the sites are very low (~1Rs) and close to the western limb, while the latter has a relatively higher and wider acceleration sites. (ii) When the proton acceleration starts from the higher energy, a SPE tends to be a relatively weak event (<1000pfu), in spite of its associated CME is relatively stronger than previous group. (iii) The SPEs categorized by the simultaneous proton acceleration in whole energy range within 10 minutes, tend to show the weakest proton flux in spite of strong related eruptions. Their acceleration heights are very close to the locations of type II radio bursts. Based on those results, we suggest that the different characteristics of the four groups are mainly due to the different mechanisms governing the acceleration pattern and interval, and different condition such as the acceleration location.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.4
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• pp.839-846
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• 1994

This paper presents a simultaneous solution algorithm for one-dimensional unsteady flow routing through a dendritic channel system. This simulations solution algorithm is based on the double-sweep method and utilizes separate recursion equations for continuity, momentum and energy equations for each of the individual components of a dendritic channel system. Through separate recursion equations for each of the components. the new algorithm converts a dendritic channel network problem into a single-channel problem. The new algorithm is utilized in conjunction with a linearized unsteady flow model using full dynamic flow equations. The required computer storage for the coefficient matrix of the whole system is reduced significantly from the $2N{\times}2N$ matrix to a $2N{\times}4$ matrix, where N is the number of cross sections used in the computation of flow variables in a dendritic channel system. The algorithm presented in this paper provides an efficient and accurate modeling of unsteady flow events through a dendritic channel system.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.48.3-49
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• 2016

We have five different types of observation modes with regard to the Deep Ecliptic Patrol of the Southern Sky (DEEP-South); Opposition Census (OC) for targeted photometry, Sweet Spot Survey (S1) for discovery and orbit characterization of Atens and Atiras, Ecliptic Survey (S2) for asteroid family studies and comet census, NEOWISE follow-up (NW) for near simultaneous albedo measurements in the visible bands, and Target of Opportunity (TO) observation for follow-up either for unpredictable events or targets of special interests. Different exposures with such different modes result in a wide range of background noise level, the number of background stars and the mover's projected speed in each image. The Moving Object Detection Program (MODP) utilizes multiple mosaic images being taken for the same target fields at different epochs at the three KMTNet sites. MODP employs existing software packages such as SExtractor (Source-Extractor) and SCAMP (Software for Calibrating Astrometry and Photometry); SExtractor generates object catalogs, while SCAMP conducts precision astrometric calibration, then MODP determines if a point source is moving. This package creates animated stamp images for visual inspection with MPC reports, the latter for checking whether an object is known or unknown. We evaluate the astrometric accuracy and efficiency of MODP using the year one dataset obtained from DEEP-South operations.

• Proceedings of the Korean Society for Language and Information Conference
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• 2001.06a
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• pp.97-109
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• 2001

이 논문은 영어와 한국어에서 나타나는 완수동사(Accomplishment Verb)와 지속부사구(durative adverbial)의 상호작용에 대해서 논한다. Smith(1991)에 의하면 완수동사가 지속부사구와 결합하면, 동사와 부사구의 의미적 자질의 충돌을 피하기 위해서 완수동사의 상변환(Apsect Shift)이 일어난다. 본 논문은 이 상변환이 영어와 한국어에서 어떻게 실현되는가를 보이고, 이를 형식의미론에서 어떻게 기술할 수 있는가를 논한다. 완수동사가 지속부사구와 결합할 때, 영어와 한국어 모두에서 닫힌 완료 관점(closed perfective viewpoint)에서 열린 관점(open viewpoint)으로 관점 변환(viewpoint shift)이 일어난다. 그러나, 한국어의 상변환과 영어의 상변환이 완전히 일치하는 것은 아니다. 한국어의 관점변환은 완료관점도 아니고 미완료 관점도 아닌, 중립관점(neutral viewpoint)으로의 변환인 반면, 영어의 관점변환은 단순히 미완료 관점으로의 변환임이 논의된다. 이 주장은 한국어의 관점변환 구문은 이질적인 다수 사건의 연속 해석(heterogeneous sequential reading of multiple events)을 허용하는 반면, 영어의 관점변환구문은 오직 동질적인 단일 사건의 동시 해석 (homogeneous simultaneous reading of a single event)만을 허용한다는 사실에 의해서 지지 된다. 본 논문은 완수동사가 지속부사구와 결합할 때 일어나는 상변환에 대한 형식의 미론 분석을 Heim ＆ Kratzer (1998)의 틀에서 제시한다. 닫힌 관점에서 열린 관점으로의 상변환은 비가시적 시제 서법 운용자(covert temporal-modal operator)인 IMP를 지속부사구의 논항으로 설정하여 설명한다. IMP는 Dowty (1979)에서 미완료상의 모순(imperfective paradox)을 해결하기 위해서 설정한 PROG를 Heim ＆ Kratzer (1998)의 틀에 맞게 수정한 것이다. IMP는 평가 세계 (evaluation world)를 현실 세계(actual world)에서 가상 관성 세계(possible inertia would)로 변화시켜서, 완수동사의 종결점(ending point)을 현실세계에서 가상의 미래 세계로 움직이는 역할을 한다. 결과적으로, IMP는 완수동사의 닫힌 완료 관점을 현실세계에서는 열린 미완료 관점으로 변환시키되, 가상 관성 세계에서는 그대로 닫힌 관점으로 유지 시키는 효과를 가진다. 한국어와 영어의 관점 변환 구문의 차이는 각 언어의 지속부사구의 어휘 목록의 전제(presupposition)의 차이로 설명된다. 본 논문은 영어의 지속부사구는 논항의 하위간격