• Journal of Information Technology Services
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• v.21 no.3
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• pp.27-42
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• 2022

Open Data is an essential resource for the data industry. 'Act On Promotion Of The Provision And Use Of Public Data', enacted on July 30, 2013, mandates public institutions to manage the quality of Open Data and provide it to the public. Via such a legislation, the legal basis for the public to Open Data is prepared. Furthermore, public institutions are prohibited from developing and providing open data services that are duplicated or similar to those of the private sector, and private start-ups using open data are supported. However, as the demand for Open Data gradually increases, the cases of refusal to provide or interruption of Open Data held by public institutions are also increasing. Accordingly, the 'Open Data Mediation Committee' is established and operated so that the right to use data can be rescued through a simple dispute mediation procedure rather than complicated administrative litigation. The main issues dealt with in dispute settlement so far are usually the rights of third parties, such as open data including personal information, private information such as trade secrets, and copyrights. Plus, non-open data cannot be provided without the consent of the information subject. Rather than processing non-open data into open data through de-identification processing, positive results can be expected if consent is provided through active rights processing of the personal information subject. Not only can the Public Mydata Service be used by the information subject, but Open Data applicants will also be able to secure higher quality Open Data, which will have a positive impact on fostering the private data industry. This study derives a plan to establish a rights processing platform to enhance the usability of Open Data, including private information such as personal information, trade secrets, and copyright, which have become an issue when providing Open Data since 2014. With that, the proposals in this study are expected to serve as a stepping stone to revitalize private start-ups through the use of wide Open Data and improve public convenience through Public MyData services of information subjects.

• Journal of Veterinary Science
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• v.24 no.1
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• pp.11.1-11.14
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• 2023

Background: Peripheral blood mononuclear cells (PBMCs) are commonly used to assess in vitro immune responses. However, PBMC isolation is a time-consuming procedure, introduces technical variability, and requires a relatively large volume of blood. By contrast, whole blood assay (WBA) is faster, cheaper, maintains more physiological conditions, and requires less sample volume, laboratory training, and equipment. Objectives: Herein, this study aimed to develop a porcine WBA for in vitro evaluation of immune responses. Methods: Heparinized whole blood (WB) was diluted (non-diluted, 1/2, 1/8, and 1/16) in RPMI-1640 media, followed by phorbol myristate acetate and ionomycin. After 24 h, cells were stained for interferon (IFN)-γ secreting T-cells followed by flow cytometry, and the supernatant was analyzed for tumor necrosis factor (TNF)-α. In addition, diluted WB was stimulated by lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (poly I:C), reference strain KCTC3557 (RS), field isolate (FI), of heat-killed (HK) Streptococcus suis, and porcine reproductive and respiratory syndrome virus (PRRSV). Results: The frequency of IFN-γ⁺CD3⁺ T-cells and concentration of TNF-α in the supernatant of WB increased with increasing dilution factor and were optimal at 1/8. WB TNF-α and interleukin (IL)-10 cytokine levels increased significantly following stimulation with LPS or poly I:C. Further, FI and RS induced IL-10 production in WB. Additionally, PRRSV strains increased the frequency of IFN-γ⁺ CD4^-CD8⁺ cells, and IFN-γ was non-significantly induced in the supernatant of re-stimulated samples. Conclusions: We propose that the WBA is a rapid, reliable, and simple method to evaluate immune responses and WB should be diluted to trigger immune cells.

• The Korean Journal of Archival Studies
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• no.75
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• pp.299-328
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• 2023

This study aims to examine the current procedure of the adequacy review system of public standards for records management developed and disseminated by the National Archives of Korea and to explore improvements to utilize them as basic data to solidify the operation of standards. The study identified the shortcomings of the system with examples of international standards and Korean industrial standards. It also analyzed the history of standardization promoted by the National Archives of Korea. The analysis showed that most of them are simple revisions, such as changes of terminology and standard names according to law amendment. The frequency of standardization by standard also showed a significant difference. In addition, it was found that the adequacy review process or results were not disclosed clearly. As such, the study proposes various improvements to solve these problems, namely, improving standardization for the records management system, establishing detailed procedures for deliberation, and establishing evaluation standards for adequacy reviews.

• Clinics in Shoulder and Elbow
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• v.25 no.4
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• pp.311-320
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• 2022

Background: The outcomes of patients 50-55 years old or younger undergoing prosthetic shoulder arthroplasty (PSA) may not generalize to younger patients. We report outcomes following PSA in a consecutive series of patients 40 years or younger. We hypothesize that total shoulder arthroplasty (TSA) provides better outcome and durability than resurfacing hemiarthroplasty (RHA). Methods: Patients were stratified by diagnosis and surgical procedure performed, RHA or TSA. Active range of motion and self-assessed outcome were evaluated preoperatively and at final follow-up. Results: Twenty-nine consecutive PSAs were identified in 26 patients, comprising 9 TSAs and 20 RHAs, with a minimum of 2-year follow-up. Twelve PSAs were performed for chondrolysis. Mean active forward elevation, abduction, external rotation, and internal rotation improved significantly (p<0.001 for all). Mean pain score improved from 6.3 to 2.1, Simple Shoulder Test from 4.0 to 9.0, and American Shoulder and Elbow Surgeons score from 38 to 75 (p<0.001 for all). Patients undergoing RHA and TSA had similar outcomes; but three RHAs required revision, two of these within 4 years of implantation. Four of five patients undergoing revision during the study period had an original diagnosis of chondrolysis. Conclusions: PSA in young patients provides substantial improvement in active range of motion and patient reported outcomes irrespective of diagnosis and glenoid management. However, patients undergoing RHA, especially for chondrolysis, frequently require subsequent revision surgery, so that RHA should be considered with caution in young patients and only after shared decision-making and counsel on the risk of early revision to TSA.

• Analytical Science and Technology
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• v.36 no.4
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• pp.180-190
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• 2023

The presence of process related impurities in any drug or the drug product was associated with its safety, stability and efficacy. The overall literature survey proved that there is no method published on the assessment of process related impurities in brigatinib. In this study, a simple, reliable and stable HPLC qualitative method was reported for quantification of process related impurities with easy and quick extraction procedure. The impurities along with standard brigatinib was resolved on Lichrospher^® C18 (250 mm × 4.6 mm; 5 ㎛ particle size) column in room temperature using methanol, acetonitrile, pH 4.5 phosphate buffer in 55:25:20 (v/v) at 1.0 mL/min as mobile phase and UV detection at 261 nm. The method produces well resolved peaks at retention time of 4.60 min, 12.28 min, 3.37 min, 7.34 min and 8.39 min respectively for brigatinib, impurity A, B, C and D. The method produces a very sensitive detection limit of 0.0065 ㎍/mL, 0.0068 ㎍/mL, 0.0053 ㎍/mL and 0.0058 ㎍/mL for impurity A, B, C and D respectively with calibration curve linear in the concentration range of 22.5-135 ㎍/mL for brigatinib and 0.0225-0.135 ㎍/mL for impurities. The method produces all the validation parameters under the acceptable level and doesn't produces any considerable changes in peak area response while minor changes in the developed method conditions. The method can effectively resolve the unknown stress degradation products along with known impurities with less % degradation. The method can efficiently resolve and quantify the impurities in formulation and hence can suitable for the routine quality analysis of brigatinib in raw material and formulation.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.05a
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• pp.465-469
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• 2003

Microlens arrays were fabricated using a novel fabrication technology based on the exposure of a PMMA (Polymethylmethacrylate) sheet to deep X-rays and subsequent thermal treatment. X-ray irradiation causes the decrease of molecular weight of PMMA, which in turn decreases the glass transition temperature and consequently causes a net volume increase during the thermal cycle resulting in a swollen microlens. A new physical modeling and analyses for microlens formation were presented according to experimental procedure. A simple analysis based on the new model is found to be capable of predicting the shapes of microlens which depend on the thermal treatment. For the replication of microlens arrays having various diameters with different foci on the same surface, the hot embossing and the microinjection molding processes has been successfully utilized with a mold insert that is fabricated by Ni-electroplating based on a PMMA microstructure of microlenses. Fabricated microlenses showed good surface roughness with the order of 1 nm.

• Journal of the Korean Geotechnical Society
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• v.23 no.4
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• pp.133-147
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• 2007

Downhole seismic method is very economic and easy of operation because it uses only one borehole and simple surface source to obtain the shear wave velocity ($V_s$) profile of a site. Even though it is widely used by the site investigation companies, universities and institutes, however, the $V_s$ profile determined by downhole seismic method has often low reliability due to employment of wrong combinations of field losing equipment and interpretation method and deficiency of experience. Round robin test was performed and testing equipment and procedure were compared. Adequate downhole seismic testing equipment was constructed based on the comparison and verification study of the round robin test. The data acquisition and software interpretation were also developed for automation and quick test in field. Finally, the effectiveness and applicability were verified through the field test by using the constructed testing system.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1A
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• pp.25-37
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• 2008

The capacity spectrum method (CSM) is a simple and graphical seismic analysis procedure. Originally, it has been developed for buildings, but now its applicability has been extended to bridge structures. It is based on the capacity curve estimated by pushover analysis and demand spectrum reduced from linear elastic design spectrum by using effective damping or strength reduction factor. In this paper, the inelastic demand spectrum as the reduced demand spectrum is calculated from the linear elastic design spectrum by using the several formulas for the strength reduction factor. The effects of the strength reduction factor for the capacity spectrum analysis are evaluated for 3 types of symmetric and asymmetric bridge structures. To investigate an accuracy of the CSM which several formulas for strength reduction factor were applied, the maximum displacements estimated by the CSM are compared with the results obtained by nonlinear time history analysis for 8 artificially generated earthquakes. The maximum displacements estimated by the CSM using the SJ formula among the several strength reduction factors provide the most accurate agreement with those calculated by the inelastic time history analysis.

• Computers and Concrete
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• v.32 no.5
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• pp.465-474
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• 2023

The point load test (PLT) is a widely-used alternative method in the field to determine the uniaxial compressive strength due to its simple testing machine and procedure. The point load test index can estimate the uniaxial compressive strength through conversion factors based on the rock types. However, the mechanism correlating these two parameters and the influence of the mechanical properties on PLT results are still not well understood. This study proposed a theoretical model to understand the mechanism of PLT serving as an alternative to the UCS test based on laboratory observation and literature survey. This model found that the point load test is a self-confined compression test. There is a compressive ellipsoid near the loading axis, whose dilation forms a tensile ring that provides confinement on this ellipsoid. The peak load of a point load test is linearly positive correlated to the tensile strength and negatively correlated to the Poisson ratio. The model was then verified using numerical and experimental approaches. In numerical verification, the PLT discs were simulated using flat-joint BPM of PFC3D to model the force distribution, crack propagation and BPM properties' effect with calibrated micro-parameters from laboratory UCS test and point load test of Berea sandstones. It further verified the mechanism experimentally by conducting a uniaxial compressive test, Brazilian test, and point load test on four different rocks. The findings from this study can explain the mechanism and improve the understanding of point load in determining uniaxial compressive strength.

• Molecules and Cells
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• v.46 no.9
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• pp.538-544
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• 2023

The formation of uniform vitreous ice is a crucial step in the preparation of samples for cryogenic electron microscopy (cryo-EM). Despite the rapid technological progress in EM, controlling the thickness of vitreous ice on sample grids with reproducibility remains a major obstacle to obtaining high-quality data in cryo-EM imaging. The commonly employed classical blotting process faces the problem of excess water that cannot be absorbed by the filter paper, resulting in the formation of thick and heterogeneous ice. In this study, we propose a novel approach that combines the recently developed nanowire self-wicking technique with the classical blotting method to effectively control the thickness and homogeneity of vitrified ice. With simple procedures, we generated a copper oxide spike (COS) grid by inducing COSs on commercially available copper grids, which can effectively remove excess water during the blotting procedure without damaging the holey carbon membrane. The ice thickness could be controlled with good reproducibility compared to non-oxidized grids. Incorporated into other EM techniques, our new modification method is an effective option for obtaining high-quality data during cryo-EM imaging.