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Paternity test in dogs by microsatellite allele analysis (Microsatellite 대립유전자 분석을 통한 개에서의 친자감별)

  • Chae, Young-jin;Kim, Dong-keon;Kim, Hana;Lee, Moon-han;Hwang, Woo-suk;Lee, Byoung-chun;Youn, Hwa-young;Lee, Hang
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.213-219
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    • 1999
  • Microsatellite allele analysis has been used for individual identification and paternity test. In the present study, the biological father of three puppies was determined by using microsatellite allele amplification analysis. The mother bitch of the litter was a Poongsan dog. The three stud dogs that could have inseminated the bitch, by being in the same residence, were a white Poosan dog, a mixed breed, and a white Jindo dog. DNA was obtained from all the relevant dogs by buccal swabbing. Four loci of tetranucleotide repeat microsatellite were PCR-amplified, and analyzed by polyacrylamide gel electrophoresis and silver staining. The results of genotyping unambigously assigned the Poongsan dog as the biological father. There was no evidence of superfecundation. Therefore, the present study demonstrated the usefulness of microsatellite allele analysis as a simple, efficient method of paternity test in dogs.

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Oxygen Release from Peroxide Injected into Soil/Sediment (토양/퇴적물에 주입한 과산화물에서 발생되는 산소 배출)

  • Han, Kyungmin;Kim, Geonha
    • Journal of Korean Society on Water Environment
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    • v.26 no.1
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    • pp.156-159
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    • 2010
  • Peroxide is used frequently to provide electron acceptors to aerobes for the purpose of in situ bioremediation of contaminated soil/sediment. In this study, oxygen release rate of peroxides and factors affecting on dissolution and diffusion of oxygen into pore water were evaluated. Peroxides studied in this study were magnesium peroxide ($MgO_2$), calcium peroxide ($CaO_2$), and sodium percarbonate ($Na_2CO_3{\cdot}1.5H_2O_2$). $Na_2CO_3{\cdot}1.5H_2O_2$ showed the highest oxygen release rate per unit mass and the shortest release duration time among three peroxides. A simple first-order decay model for predicting the release rate of oxygen from peroxide into pore water was presented and used to fit the experimental data. The first order oxygen release rate constants k for $MgO_2$, $CaO_2$ and $Na_2CO_3{\cdot}1.5H_2O_2$ were 0.45 /hr, 3.22 /hr and 134 /hr, respectively. If $MgO_2$ was mixed with clay, oxygen release rate was lowered significantly mainly due to limitation of contact area and diffusion, implying that oxygen can be provided to the indigenous aerobes for the extended period of time.

The Improved Artificial Trap Baits (통발용 인공미끼의 개발)

  • 염말구
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.34 no.2
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    • pp.185-190
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    • 1998
  • The aim of this study was to develop the artificial trap baits being able to replace the natural anchovy or sardine baits. The fishing trials for evaluating the fishing efficiencies of the artificial baits were conducted with commercial traps at coastal areas near Tongyoung, southern Korea. The preferable artificial baits which were made of minced anchovy, sardine, mackerel, or fish surplus products mixed with wheat flour into dry or wet pelleted or crumbled types, were showed some merits as the following. \circled1 Fishing efficiencies of the artificial baits were same or little higher level than that of the natural bait anchovy; \circled2 bait cost were same or little lower level; \circled3 dry artificial baits stocked in the room condition showed good fishing efficiencies; \circled4 making of artificial baits were very simple; \circled5 there were no harm to the fish and little pollution to the environment; \circled6 the releasing rate of attractants in the artificial or natural bait showed similar patterns. The artificial baits using the fish by-products which were vacuum freeze-drying extract of boiled anchovy or tunas extract, showed lower catches in the conger eel, Coner miriaster, but similar catches in the crabs, mainly Charybdis japonica, to the those of natural anchovy bait.

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A Study on the Modern Fashion Reflected Art of the Tang Dynasty (당대(唐代) 예술(藝術)이 반영(反映)된 현대(現代)패션에 관(關)한 연구(硏究) - 1998년(年)부터 2000년(年) Collection을 중심(中心)으로 -)

  • Kan, Ho-Sup;Suh, Yun-Hee
    • Journal of Fashion Business
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    • v.4 no.4
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    • pp.55-70
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    • 2000
  • The purpose of this thesis is to suggest the new orientalism fashion through the comprehensive approach to analyze the art of the Tang dynasty, China. Tang dynasty has high culture, economy, polity and art. Also the character of Tang's culture is very international. Therefore that point is coincide with fusion culture in the early 2000's. As a method of accomplishing this research, the documents and fashion magazines related to the art and fashion were examined. Through analyzing the art in the Tang's related to fusion culture, reach a conclusion that the modern fashion has four characters. 1. The cause of high culture and economy, the trend of fashion was decorative 2. The cause of realism, the trend of fashion were modern and simple. 3. The cause of rhythmical line, the trend of fashion had natural drape. 4. The cause of globalism, the trend of fashion mixed east with west This research keep going for showing new orientalism and coinciding with 21th century's trend.

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A Study on the Design Changes and Characteristics of Modern Knitwears - Focusing on the1920's After - (현대(現代)니트웨어 디자인의 변천(變遷)과 특성(特性)에 관(關)한 연구(硏究) - 1920년대(年代) 이후(以後)를 중심(中心)으로 -)

  • Choi, Kyung-Hee;Lee, Soon-Hong
    • Journal of Fashion Business
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    • v.3 no.4
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    • pp.113-129
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    • 1999
  • The purpose of this study is to discover the changes and characteristis of modern knitwears from the 1920's to the 1990's. The result of this study is as follows: The popular knitwears in the 1920's had a simple boxy-line and modern unisex image with a sporty look. The knitwears in the 1930's-1950's had seen more short, feminine detail and texture, fit silhouette with syntheyic fibers. In the 1960's, the knitwears of sporty look and unisex style was popularized by young peaples, and crochet with handkniting by the fiber artists was popularized. In the 1970's-80's, the layered looks and unisex styles of knitwears were popularized by influence of a ecology mood and a woman movemant. And various patterns was developed with the computer machine. The items of outer knitwear was increased by the use of fancy yarns and the knitwears as the total fashion was popularized. In the 1990s, the knitwears had a very forceful characteristics by the mixed style and the technique of design developed into the post-modernism. The key words of the character of the modern knitwears are funtional pursuit, unisex, high value, hightechnique, art. By the change of the life style and the develope of technical innovation, the knitwears will be the key item to leading the fashion in the 21th.

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Elemental Analysis of Coal by Inductively Coupled Plasma Mass Spectrometry (誘導結合플라스마 質量分析法에 의한 石炭의 元素 分析)

  • 이용근;고인형;이동수
    • Journal of Korean Society for Atmospheric Environment
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    • v.8 no.3
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    • pp.155-161
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    • 1992
  • A simple, fast yet sensitive method is described for the determination of fifty elements in coal by inductively coupled plasma mass spectrometry. The method involves complete dissolution of coal with mixed acid $(HNO_3, HF, HClO_4)$ in hish pressure Teflon bomb and subsequent inductively coupled plasma mass spectrometric(ICP-MS) measurement. The accuracy of the method, being evaluated by the analysis of NIST SRMs (1632a, 1632b) is better than 20% RSD for most elements. The limits of detection defined by two times $\sigma$ (standard deviation of operational blank) are in order of sub-ppm to ppm, which are low enough to quantitate most elements. However, the determiantions for few elements such as V, As, Se are severely interfered by molecular ions such that their accurate determiantions are not possible. Analytical results for twentyon coals from eight countries including six ones world major coal producing, Korea, Japan, China, I.C.S., U.S.A., Canada, Australia, and South Africa are presented. While the results for major elements agree well with the existing ones, those for a few trace elements disagree considerably. The existing values are considerably higher. It is not possible to judge which are more accurate. However, the exisisting values are more likely to be errorous inasmuch as they are obtained without using high purity reagents and clean laboratory techniques.

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A Design of Multi format HD LCD Monitor for Broadcasting (방송용 Multi format HD LCD Monitor의 설계)

  • Han, Sung-Il;Jun, Eung-Sup;Noh, Hyung-Il
    • Journal of the Korea Society of Computer and Information
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    • v.15 no.3
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    • pp.37-43
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    • 2010
  • In this study, the multi format 8.4" LCD monitor for broadcasting has been proposed by the simple circuit design and the efficient processing technique. The small sizing technique, the mixed-type video signal processing technique, the emerging skill of multi-format HD signal to one port, the dividing technique between the analog and digital signal, the embedded audio signal developing technique are the key researches of this study. The proposed multi format 8.4" LCD monitor has been focused on the broadcasting field and has been supposed to have the efficiency at that field.

Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry

  • Ahn, Jang-Hyuk;Kwak, Byung-Man;Park, Jung-Min;Kim, Na-Kyeoung;Kim, Jin-Man
    • Food Science of Animal Resources
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    • v.34 no.6
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    • pp.749-756
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    • 2014
  • A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving.

MODEL DUST ENVELOPES AROUND SILICATE CARBON STARS (규산염탄소항성의 먼지층 모형)

  • Suh Kyung-Won
    • Journal of Astronomy and Space Sciences
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    • v.23 no.1
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    • pp.11-18
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    • 2006
  • We have modeled dust envelopes around silicate carbon stars using optical properties for a mixture of amorphous carbon and silicate dust grains paying close attention to the infrared observations of the stars. The 4 stars show various properties in chemistry and location of the dust shell. We expect that the objects that fit a simple detached silicate dust shell model could be in the transition phase of the stellar chemistry. For binary system objects, we find that a mixed dust chemistry model would be necessary.

An Analysis on the Research Method of Elderly Residents' Opinion towards the Physical Environments of the Facilities for the Elderly : Focusing on Foreign Academic Journal Articles since 1990 (노인시설의 물리적 환경에 대한 거주노인 의견 조사방법의 분석 : 1990년 이후 해외 학술논문자료를 중심으로)

  • Lee, Min-Ah
    • Journal of Families and Better Life
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    • v.33 no.2
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    • pp.35-51
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    • 2015
  • The purpose of this study was to provide information for research about residents' opinion toward the physical environments of elderly facilities, through the analysis and investigation on the research methodology of foreign academic journal articles from 1990 to 2014. The study results were as follows: Firstly, purposive sampling was a large majority of both facilities and elderly residents. In quantitative studies, many researchers have conducted simple random, cluster, or stratified sampling. Diverse facilities in area, size, location, and etc. should be considered for participation. The qualifications for residents' participation should be considered as well, so that they all could have autonomy for study participation. Secondly, questionnaire and semi-structured guide were likely to be used in independent and resident care facilities. On the other hand in assisted living and long-term care facilities, open questions and visual material were used as well. A compatible scale should be developed so that elderly having variable functional level could participate independently in the study. Thirdly, in data collection process, compliance with research ethics and well trained interviewer's skill were important for residents' active responses and minimization of response errors. Enough research period of time and mixed study in data collection will decrease the response error.