[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5851/kosfa.2014.34.6.749

Rapid Determination of L-carnitine in Infant and Toddler Formulas by Liquid Chromatography Tandem Mass Spectrometry

Ahn, Jang-Hyuk (KOTITI Testing & Research Institute)
Kwak, Byung-Man (Food Safety Center, Research and Development Institute, Namyang Dairy Co., Ltd.)
Park, Jung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Kim, Na-Kyeoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)

Publication Information

Food Science of Animal Resources / v.34, no.6, 2014 , pp. 749-756 More about this Journal

Abstract

A rapid and simple analytical method for L-carnitine was developed for infant and toddler formulas by liquid chromatography tandem mass spectrometry (LC-MS/MS). A 0.3 g of infant formula and toddler formula sample was mixed in a 50 mL conical tube with 9 mL water and 1 mL 0.1 M hydrochloric acid (HCl) to chemical extraction. Then, chloroform was used for removing a lipid fraction. After centrifuged, L-carnitine was separated and quantified using LC-MS/MS with electrospray ionization (ESI) mode. The precursor ion for L-carnitine was m/z 162, and product ions were m/z 103 (quantitative) and m/z 85 (qualitative), respectively. The results for spiked recovery test were in the range of 93.18-95.64% and the result for certified reference material (SRM 1849a) was within the range of the certificated values. This method could be implemented in many laboratories that require time and labor saving.

Keywords

L-carnitine; LC-MS/MS; analytical method; infant formula; toddler formula;

Citations & Related Records

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