• Title/Summary/Keyword: simple mixed

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Rapid Identification of Lactobacillus plantarium in Kimchi Using Polymerase Chain Reaction

  • Kim, Tae-Woon;Min, Sung-Gi;Choi, Dong-Hun;Jo, Jae-Sun;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.881-884
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    • 2000
  • A polymerase chain reaction (PCR) was performed to rapidly identify Lactobacillus plantarum from type strains and kimchi samples. The PCR experiments were carried out using specific oligonucleotide primer sets based on the 16S rRNA gene sequences of L. plantarum. The expected DNA amplificate of 419 bp was obtained when either purified DNA or whole cells of L. plantarum strains reacted with LP primers, yet not with any of the other strains. The PCR product was confirmed by DNA sequencing. Accordingly, since the PCR method used is simple, specific, and rapid, it will be useful for monitoring and evaluation L. plantarum in the mixed microbial population found in kimchi.

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Random Effects Models for Multivariate Survival Data: Hierarchical-Likelihood Approach

  • Ha Il Do;Lee Youngjo;Song Jae-Kee
    • Proceedings of the Korean Statistical Society Conference
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    • 2000.11a
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    • pp.193-200
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    • 2000
  • Modelling the dependence via random effects in censored multivariate survival data has recently received considerable attention in the biomedical literature. The random effects models model not only the conditional survival times but also the conditional hazard rate. Systematic likelihood inference for the models with random effects is possible using Lee and Nelder's (1996) hierarchical-likelihood (h-likelihood). The purpose of this presentation is to introduce Ha et al.'s (2000a,b) inferential methods for the random effects models via the h-likelihood, which provide a conceptually simple, numerically efficient and reliable inferential procedures.

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Simultaneous Analysis of Gasonline and Chlorinated Solvents by GC/FID-SPME

  • 안상우;이시진;장순웅
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.350-353
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    • 2004
  • This study was conducted for a rapid and simple method using GC/FID and SPME to dectermin gasonline and chlorinated solvents simultaneity. A sodium chloride concentration of 25%(vol/w) combined with such as magnetic stirried, an absorption time of 20min, an extraction temperature of 4$0^{\circ}C$, the volume of minimized 50mL of gaseous phase and a desorption time of 5min pleprovided the greatest sensitivity while maintaining analytical efficiency. Analytical parameter such as linearity was also evaluated. The linear range extend from 30 to 500ppb. The results of chlorinated solvents and gasoline mixed samples showed that solvents have been completely removed from the sample preparation step and more accurated than those obtained by the other methods.

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APPLICATION OF NEURAL NETWORK FOR THE CLOUD DETECTION FROM GEOSTATIONARY SATELLITE DATA

  • Ahn, Hyun-Jeong;Ahn, Myung-Hwan;Chung, Chu-Yong
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.34-37
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    • 2005
  • An efficient and robust neural network-based scheme is introduced in this paper to perform automatic cloud detection. Unlike many existing cloud detection schemes which use thresholding and statistical methods, we used the artificial neural network methods, the multi-layer perceptrons (MLP) with back-propagation algorithm and radial basis function (RBF) networks for cloud detection from Geostationary satellite images. We have used a simple scene (a mixed scene containing only cloud and clear sky). The main results show that the neural networks are able to handle complex atmospheric and meteorological phenomena. The experimental results show that two methods performed well, obtaining a classification accuracy reaching over 90 percent. Moreover, the RBF model is the most effective method for the cloud classification.

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AN APPLICATION OF LAGRANGIAN RELAXATION ALD SUBGRADIENT METHOD FOR A DYNAMIC UNCAPAITATED FACILITY COCATION PROBLEM

  • Song, Jae-Wook;Kim, Sheung-Kown
    • Journal of the Korean Operations Research and Management Science Society
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    • v.13 no.2
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    • pp.47-58
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    • 1988
  • The dynamic uncapacitated facility location model is formulated by a mixed integer programming. It has the objective of minimizing total discounted costs for meeting demands specified in different time periods at various demand centers. Costs include those for operation of facilities to demand centers and a fixed cost associated with the capital investment. The problem is decomposed into two simple Lagrangian relaxed subproblems which are coordinated by Lagrangian multipliers. We explored the effect of using the subgradient optimization procedure and a viable solution approach is proposed. Computational results are presented and further research directions are discussed.

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A Heuristic Method for Resolving Circular Shareholdings of Korean Large Business Groups (대규모 기업집단의 순환출자 해소를 위한 휴리스틱 기법)

  • Park, Chan-Kyoo
    • Journal of the Korean Operations Research and Management Science Society
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    • v.38 no.4
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    • pp.65-78
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    • 2013
  • Circular shareholding is established when at least three member firms in a business group hold stock in other member firms and form a series of ownership in a circular way. Although there have been many studies which investigated a negative effect of circular shareholding on firm's value, few studies have discussed how to resolve the problem given complicated ownership structures of large business groups. This paper is based on a mixed integer programming model, which was proposed in the author's previous research and can identify the ownership share divested in order to resolve circular shareholding. Since the optimization model becomes too complicated for large business groups and requires a sophisticated software to solve it, we propose a simple heuristic method that can find a good approximate solution to the model. Its applications to twelve Korean large business groups show that the heuristic method is not just computationally attractive but also provides near-optimal solutions in most cases.

Parametric study on equivalent damping ratio of different composite structural building systems

  • Farghaly, Ahmed Abdelraheem
    • Steel and Composite Structures
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    • v.14 no.4
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    • pp.349-365
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    • 2013
  • Structures consisting of concrete and steel parts, which are irregular in damping ratios are investigated. This investigation is a code-based seismic design of such structures. Several practical difficulties encountered, due to inherent differences in the nature of dynamic response of each part, and the different damping ratios of the two parts. These structures are irregular in damping ratios and have complex modes of vibration so that their analysis cannot be handled with the readily available commercial software. Therefore, this work aims to provide simple yet sufficiently accurate constant values of equivalent damping ratios applied to the whole structure for handling the damping irregularity of such structures. The results show that the equivalent damping ratio changes with the height of the building and the kind of the structural system, but it is constant for all accelerations values. Thus, available software SAP2000 applied for seismic analysis, design and the provisions of existing seismic codes. Finally, evaluation of different kinds of structural system used in this research to find the most energy dissipating one found by finding the best value of quality coefficient.

Influence of elastic T-stress on the growth direction of two parallel cracks

  • Li, X.F.;Tang, B.Q.;Peng, X.L.;Huang, Y.
    • Structural Engineering and Mechanics
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    • v.34 no.3
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    • pp.377-390
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    • 2010
  • This paper studies fracture initiation direction of two parallel non-coplanar cracks of equal length. Using the dislocation pile-up modelling, singular integral equations for two parallel cracks subjected to mixed-mode loading are derived and the crack-tip field including singular and non-singular terms is obtained. The kinking angle is determined by using the maximum hoop stress criterion, or the ${\sigma}_{\theta}$-criterion. Results are presented for simple uniaxial tension and biaxial loading. The biaxiality ratio has a noticeable influence on crack growth direction. For the case of biaxial tension, when neglecting the T-stress the crack branching angle is overestimated for small crack inclination angles relative to the largest applied principal stress direction, and underestimated for large crack inclination angles.

Standard Error of Empirical Bayes Estimate in NONMEM$^{(R)}$ VI

  • Kang, Dong-Woo;Bae, Kyun-Seop;Houk, Brett E.;Savic, Radojka M.;Karlsson, Mats O.
    • The Korean Journal of Physiology and Pharmacology
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    • v.16 no.2
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    • pp.97-106
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    • 2012
  • The pharmacokinetics/pharmacodynamics analysis software NONMEM$^{(R)}$ output provides model parameter estimates and associated standard errors. However, the standard error of empirical Bayes estimates of inter-subject variability is not available. A simple and direct method for estimating standard error of the empirical Bayes estimates of inter-subject variability using the NONMEM$^{(R)}$ VI internal matrix POSTV is developed and applied to several pharmacokinetic models using intensively or sparsely sampled data for demonstration and to evaluate performance. The computed standard error is in general similar to the results from other post-processing methods and the degree of difference, if any, depends on the employed estimation options.

A Study on the Proposed Faceted Scheme for Literature (문학류를 위한 새로운 분류체계에 대한 연구)

  • 정해성
    • Journal of Korean Library and Information Science Society
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    • v.34 no.2
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    • pp.273-296
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    • 2003
  • Because all the subjects may become the targets of classification, it is necessary to change the enumerated into the faceted scheme. This study is to confirm that possibility of change and to proposed the new faceted structure scheme(faceted classification) based on the Literature. The proposed schemes are : 1) facet structure is simple, 2) meaning of facet is clearness, 3) because using mixed notation, it is complicated.

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