• Title/Summary/Keyword: silver-alginate

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Antibacterial Properties of Silver-alginate/PVP Nanofiber (은-알지네이트/PVP 나노섬유의 항균 특성)

  • Choi, Yoo-Sung;Min, Kyung-Du;Yoon, Doo-Soo
    • Applied Chemistry for Engineering
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    • v.24 no.4
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    • pp.416-422
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    • 2013
  • In order to incorporate silver ions into the alginate, silver-alginate was prepared with aqueous solutions of silver nitrate. In the study, the silver-alginate was prepared by blending with poly vinylpyrrolidone solutions and the electrospinning was performed by using this blend solution. Antibacterial properties of silver-alginate/PVP solutions were estimated for Escherichia coli and Staphylococcus aureus by the colony counting test. Electrospinning conditions of silver-alginate/PVP solution were the tip-to-collector distance of 22 cm, the flow rate of the solution at 0.01 mL/min, and the voltage at 26 kV. The form and size of silver-alginate/PVP nanofibers were estimated by SEM and Image J. The average diameter of the electrospun SA5P15 fibers was 124 nm and showed a narrow diameter distribution. The reduction of bacteria for SA5P15 exhibited 99.9% after 24 h.

Preparation and Characterization of Alginate-Chitosan Microsphere for Controlled Delivery of Silver Sulfadiazine (설파디아진은의 방출제어를 위한 알지네이트-키토산 미립구의 제조 및 특성)

  • Cho, Ae-Ri
    • Journal of Pharmaceutical Investigation
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    • v.31 no.2
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    • pp.101-106
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    • 2001
  • Alginate-chitosan (anion-cationic polymeric complex) was prepared to control the release rate of silver sulfadiazine (AgSD). Na-alginate (2%) solution containing AgSD was gelled in $CaCl_2$ solution. The gel beads formed were immediately encapsulated with chitosan (CS). The gel matrix and membrane were then reinforced with chondroitin-6-sulfate (Ch6S). Release rate of AgSD from the gel matrix was investigated by placing alginate beads in the sac of cellulose membrane simmered in HEPES-buffer solution. The concentration of AgSD released was analyzed by UV at 264 nm. Incorporation capacity of AgSD in Ca-alginate gel was more than 90%. Alginate-Ch6S-CS could control the release rate of AgSD. The amount of AgSD release was dependent on the AgSD loading dose. Incorporation of tripolyphosphate (polyanionic crosslinker) onto the alginate-Ch6S-CS bead increased the release rate of AgSD. Collagen-coating had no influence on the AgSD release rate. Alginate-Ch6S-CS beads with a sufficiently high AgSD encapsulation were capable of controlling the release of the drug over 10 days. In summary, alginate-Ch6S-CS beads could be used as a sustained delivery for AgSD and provide local targeting with low silver toxicity and patient discomfort.

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Preparation of Antibacterial Agent using Alginate and Its Antibacterial Effect (알긴산염을 이용한 항균제의 제조 및 항균효과)

  • 이학성;서정호
    • KSBB Journal
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    • v.17 no.1
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    • pp.63-67
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    • 2002
  • Silver-alginate and copper-alginate were prepared with Na-alginate extracted from marine brown algae(Sargassum fluitans). The antibacterial effect of Ag-alginate or Cu-alginate against Staphylococcus aureus and Escherichia coli was carried out by measuring optical density of liquid culture at 600 nm. The cell growth of Staphylococcus aureus and Escherichia coli was very active at pH 7, and was inhibited by adding Ag-alginate with more than 0.006 wt.% of silver content. The antibacterial effect of Ag-alginate against S. aureus and E. coli was better than that of Cu-alginate at the same metal concentration. The cell growth of S. aureus was less inhibitory than E. coli at the same concentration of Ag-alginate. The cell growth of S. aureus and E. coli was also influenced by the characteristics of counter ion of silver.

Preparation of Antibacterial Agent from Seaweed Extract and Its Antibacterial Effect (해조류 추출물로부터 항균제의 제조 및 항균효과)

  • LEE Hak-Sung;SUH Jung Ho;SUH Kuen-Hack
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.32-37
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    • 2000
  • Silver-alginate (Ag-alginate) was prepared with Na-alginate extracted from marine brown algae. The antibacterial effect of Ag-alginate against Staphylococcus aureus and Escherichia coli was carried out by measuring optical density of liquid culture at 600 nm. The cell growth of S. aureus and E. coli was very active at pH 7, and was inhibited by adding Ag-alginate with more than $0.006 wt.{\%}$ of stiver content. The cell growth of S. aureus .and E. coli was also influenced by the characteristics of counter Jon of silver. The cell growth of S. aureus was less inhibitory than E. coli at the same concentration of Ag-a1ginate.

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Cost-Effectiveness Analysis in Comparing Alginate Silver Dressing with Silver Zinc Sulfadiazine Cream in the Treatment of Pressure Ulcers

  • Chuangsuwanich, Apirag;Chortrakarnkij, Peerasak;Kangwanpoom, Jupaporn
    • Archives of Plastic Surgery
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    • v.40 no.5
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    • pp.589-596
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    • 2013
  • Background The treatment of pressure ulcers is complicated, given the various wound dressing products available. The cost of different treatments varies and the cost-effectiveness of each product has not been thoroughly evaluated. We compare two wound dressing protocols-alginate silver dressing (AlSD) and silver zinc sulfadiazine cream (AgZnSD) with regard to wound healing and cost-effectiveness Methods Patients with grade III or IV sacral or trochanteric pressure ulcers were eligible for this prospective, randomized controlled trial. The patients were randomized to receive one of the two dressings for an eight-week period. The criteria of efficacy were based on the Pressure Ulcer Scale for Healing (PUSH) scoring tool. The cost of treatment was also assessed. Results Twenty patients (12 women and 8 men) were randomly assigned to receive either AlSD (n=10) or AgZnSD cream (n=10). The demographic data and wound characteristics were comparable in the two groups. The two groups showed no significant difference in the reduction of PUSH score, wound size, or volume of exudate. The tissue type score was significantly lower in the AlSD group ($3.15{\pm}0.68-1.85{\pm}0.68$ vs. $2.73{\pm}0.79-2.2{\pm}0.41$; P=0.015). The cost of treatment was significantly lower in the AlSD group (377.17 vs. 467.74 USD, respectively; P<0.0001). Conclusions Alginate silver dressing could be effectively used in the treatment of grade III and IV pressure ulcers. It can improve wound tissue characteristics and is cost-effective.

Biosorption of Metal Ions by Seaweed Alginate, Polyguluronate, and Polymannuronate (알긴산, 폴리글루론산 및 폴리만뉴론산에 의한 금속이온의 흡착)

  • Jung, Dae-Young;Son, Chang-Woo;Kim, Sung-Koo;Kim, Yi-Joon;Chung, Chung-Han;Lee, Jin-Woo
    • Journal of Life Science
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    • v.19 no.5
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    • pp.553-560
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    • 2009
  • Based on $P_{1/2}$ values, relative affinities of alginate, polyguluronate, and polymannuronate for metal ions are, in order, as follows; 1) seaweed alginate: $Cu^{2+}$ > $Cd^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Zn^{2+}$ > $Sr^{2+}$ > $Ca^{2+}$ > $Co^{2+}$ >> $Cr^{6+}$ > $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, 2) polyguluronate: $Cd^{2+}$ > $Cu^{2+}$ > $Pb^{2+}$ > $Fe^{3+}$ >> $Ca^{2+}$ > $Sr^{2+}$, $Zn^{2+}$, $Co^{2+}$ >> $Mn^{2+}$ > $Cr^{6+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$, and 3) polymannuronate: $Cd^{2+}$, $Cu^{2+}$ > $Fe^{3+}$ > $Pb^{2+}$ > $Ca^{2+}$ > $Zn^{2+}$ > $Sr^{2+}$ > $Co^{2+}$ > $Cr^{6+}$ >> $Mn^{2+}$ >> $Hg^{2+}$, $Mg^{2+}$, $Rb^+$. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1 g of seaweed alginate, were measured as $363.5{\pm}45.0$, $226.3{\pm}9.2$, $1,299.4{\pm}$81.3, 500.7${\pm}$27.7, and 165.9${\pm}$11.4 mg, respectively. Amounts of the metal ions, $Cd^{2+}$, $Cu^{2+}$, $Fe^{3+}$, $Pb^{2+}$, and $Zn^{2+}$, bound to 1g of polyguluronate, were 354.5${\pm}$26.5, 177.6${\pm}$8.7, 1,288.6${\pm}$60.1, 424.0${\pm}$7.4, and 140.2${\pm}$28.5 mg, respectively, whereas those bound to 1 g of polymannuronate were 329.0${\pm}$10.3, 206.9${\pm}$1.9, 1,635.6${\pm}$11.1, 419.8${\pm}$12.6, and 251.0${\pm}$49.1 mg, respectively. Due to its higher solubility than alginate and higher affinity for metal ions than polyguluronate, polymannuronate can be used for bioremediation or biosorption of toxic and/or noble metal ions.