• Biotechnology and Bioprocess Engineering:BBE
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• 제3권2호
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• pp.87-90
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• 1998

Hemolymph is oxidized and darkens visibly during the collection from silkworm due to the activity of tyrosinase in it. Toxic quinones are produced by the oxidation and consequently inhibit the cell growth. Heat treatment can be used to prevent the oxidation ; however, the oxidation may occur during the collection of hemolymph before it is heat-treated. It makes the hemolymph collection difficult especially on a large-scale preparation. Hemolymphs collected from 257 different strains of silkworms were examined to select the slowly oxidized hemolymphs. Hemolymphs collected from mutant strains such as Lemone, TBO, Cre, Y4, and wEb showed relatively slow color changes. Oxidation rates of the hemolymphs were measured by the absorbance change using a spectrophotometer. The hemolymph of wEb showed the slowest oxidation. The absorbance of this mutant hemolymph reached the saturation value at 20$^{\circ}C$ in 450 min, whereas the total oxidation time of the wild-type (Baekokjam) hemolymph at the same temperature was 120 min. We tested if this mutant hemolymph is useful as a medium supplement for insect cell culture. Cell growth rate and final cell concentration in the medium supplemented with the wEb hemolymph were almost same as those in the medium supplemented with the wild-type hemolymph. Hemolymph is collected on a small scale by clipping the abdominal leg; however, this method is not appropriate fro large scale preparation. Centrifugation after chopping the silkworm hemolymph by a blending mixer is a more appropriate procedure for large scale collection. Slowly oxidized wEb hemolymph resulted in higher cell concentration than the wild-type hemolymph when hemolymph was collected by the large scale preparation method.

• 한국잠사곤충학회지
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• 제14권2호
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• pp.61-65
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• 1972

우수한 이화성일본계 N43호를 선출하고 이것과 우량하다고 보는 이화성 중국계 KC, C14, C20과 웅별 자별로 교배시키고 그중에서 우량한 교배종을 선출하려는 목적으로 가잠형질중 가장 중요한 견층량의 유전력의 산출에 의해 선출을 시도한 것이다. 그 결과 다음 같은 결론을 얻게 되었다. 1, KC$\times$N13에 대하여 견층량은 3교배조중 많은 편이나 유전력(h$^2$=0.26)은 낮은 편이며 또한 웅의 유전력은 자의 그것보다 높다. 2. C14$\times$N13에 대하여 견층량에 있어서는 3교배조중 중간이며 또한 유전력(h$^2$=0.3)로 중간이라 이 교배조에서만 자의 유전력이 웅보다 크게 나타나 있다. 3. 020$\times$N13에 대하여 견층량에 있어서는 3교배조중 가장 적은 편이나 유전력(h$^2$=0.35)은 가장 많은 편이다. 또 과거의 3잠기를 통하여 견층비율도 양호하고 기타 충질에서 강한 것으로 우량품종으로 기대할 수 있다고 인정되는 바가 크다.

• 미생물학회지
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• 제8권1호
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• pp.21-34
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• 1970

This experiment was undertaken to examine the injurious environment conditions for occuring of the virus disease, grasserie and cytoplasmic polyhedrosis in rearing of silk worms, to observe of cytoplsamic polyhedrosis diseased silkworms with histological preparation and to define the virus origin on the gattine and the disease of shrinked form after moulting (Okichijimi). The results obtained are as follows. 1) The grasserie in spring season rearing was remarkably infected in highly percent with 20.1 % in high temperature condition during 3rd to 4th instar, the high temperature during 1st to 2nd instar and 5th instar in 16.5% and 16.3%, respectively. In the fall season rearing, the disease was infected by the feeding of soft leaves plot in 5.3% and 4.8%, respectively with significant difference in 5% level, accordingly, it was thought to the nutritional condition is a factor in occuring of the disease. 2) In spring season rearing, the number ofl infected silk worms of cytoplasmic polyhedrosis was increased in the high temperautre and high humidity conditions, and in fall season rearing, order of the low temperature and high humidity plot, first feeding plot and feeded with hard leaves plot were found insome high infected ratio of the disease than control plot. 3) The occuring of cytoplasmic polyhedrosis was observed even in control rearing plot with the examining of anatomical and histological preparation in spring and fall. 4) It was found that the high diseased ratio of the gattine and disease of shrinked form after moulting in 21.8% of control and 93.2% in feeded with inocylated plot in the biosassay of inoculum. It was defined as a virus flacherie acoording to the Danaka and Shimizu's examine method.

• 한국잠사곤충학회지
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• 제14권1호
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• pp.37-42
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• 1972

잠병이 잠작에 미치는 영향을 조사하기 위하여 경기도 용인군 남사면 방아리, 경북 칠곡군 약목면 그리고 경북 칠곡군 북삼면의 양잠농가를 대상으로 잠병에 대한 피해율을 조사한 결과는 다음과 같다. 1) 잠병에 의한 감수율은 춘잠기에는 5.5%, 추잠기에는 8.9%이었고 년간 평균 7.2%였으며 하견을 병해견으로 환산한다면 8.3%였다. 2) Virus 다각체병과 연화병에 의한 병해율은 전발병의 치잠기에는 17.8%, 장잠기에는 82.2%로서 높은 비율을 나타내었다. 3) 평균산견량은 상자당 27.74kg이었는데 추정산견량에 대하여 잠병에 의한 감수량은 2.411kg이었다. 4) 원인불명의 감수량이 3.611kg으로서 잠병에 의한 감수량의 약 1.5배였다. 이 원인불명의 감수량은 주로 유실잠과 하견이 많았기 때문이다. 5) 잠작이 비교적 안정된 농가수는 춘기에는 54.7%, 추기에는 21.9%였다. 6) 잠병이외의 피해율은 10.70%였고 전감잠비율은 17.87%였다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 1997년도 Progress and Future Development of Sericultural Science and Technology 40th Anniversary Commemoration Symposium
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• pp.133-158
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• 1997

Since 1992, Sericulture & Entomology Research Institute(NSERI) and Kyung Hee University group screened an activity of lowering blood-glucose levels with silkworm related materials such as silkworm larvae powder, dried feces, pupae and silkworm moth in other to guide laymans to rational and educated utilization of silkworm-related materials for the treatment of diabetes mellitus. In experiments examining several silkworms in different stages and prepared in different conditions, a freeze dried silkworm powder of 5th instar 3rd day showed a higher glucose lowering activity about 20% than heat dried matured silkworm powder. Among the three dosage of 500mg, 830mg and 1,160mg in ate preliminary clinical trial, the 830mg exhibited a significant effect on postprandial blood glucose level and did and did not cause any hypoglycemic side effect. In the blood glucose lowering activity of mulberry and silkworm varieties, the Yongcheonppong and Samkwangjam showed the highest activity for lowering blood glucose levels. In experiments to see the difference in blood-glucose lowering activity between either male and female or larvae and pupae, activity of larvae was higher than that of pupae and the male was higher than female. The heating dry and artificial diet showed lower than mulberry diet and normal freeze dry of the 5th instar 3rd day. Among the sericultural products, larvae showed the highest activity. We find out the fact that effect of silkworm powder attributed to the inhibition of ${\alpha}$-glucohydrolase catalyzed reaction in the small intestine.

• Acta Via Serica
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• 제6권2호
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• pp.91-116
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• 2021

India has had a rich and diverse textile tradition since the 3rd millennium BCE. The origin of Indian textiles can be traced back to the Harappan period. Owing to the hot and humid climate in most parts of India, cotton has remained India's favourite choice of fabric for normal use. Thus, India is supposed to be the first nation to have grown, woven, and patterned cotton fabrics. Moreover, India is one of the leading cotton-growing countries in the world. The earliest occurrence of cotton thread in India is roughly datable to 4000 BCE and of dyed fabrics to about 2500 BCE. Large numbers of needles and spindle-whorls found in Harappa and other early historic sites in India reveal the prosperous state of textile production and its trade in the early period. The textile producers used a wide range of skills to process raw materials and make regionally idiosyncratic dyes, weaves, prints, and embroideries. Additionally, the silk from wild indigenous forms of silkworms was known in the Indian sub-continent roughly contemporary with the earliest clear archaeological evidence for silk in China. The analysis of thread fragments found inside a copper bangle and ornament from Harappa and steatite beads from Chanhu-daro, have yielded silk fibers dating to 2500-2000 BCE. Apart from other products, cotton and silk textiles were important export materials from India right from the Harappan period. Actually, the sea-borne trade had played an important role in the economic growth and prosperity of the Harappan civilization. Several ancient seaports in the entire coastline of India played a vital role in the maritime trade during the Harappan period and cotton and silk textiles of Indian origin have been found in various countries. The contemporary writings and epigraphy have also attested to the vast maritime trade network of India and the export of textile materials. The paper discusses in detail the origin and development of cotton and silk textile production in India through the ages and its role in maritime trade networks.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.84-92
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• 2022

White-Jade silkworm (previously also known as Baegokjam) variety is the most popular silkworm variety that produces white cocoons. In 2021, the market share of White-Jade variety in Korea is very high, accounting for 88% of the silkworm production. Daebaekjam, Dodamsilkworm, Kumkangjam, and Kumokjam varieties, which have recently been established, make white cocoons like White-Jade. In this study, we found that 5 types of HongJams produced from 5 varieties of silkworms producing white cocoons did not show any severe difference in proximate analysis. The amounts of crude proteins, the most abundant nutrient, were between 71.05 ~ 73.38%, and those of crude lipids were 13.89 ~ 14.69% in 5 types of HongJams. In addition, there was no difference between White-Jade HongJam (WJ) and Daebaekjam HongJam in amino acid compositions. The amount of unsaturated fatty acid was significantly higher in WJ than in the other four types of HongJams, but the omega-6 fatty acids/omega-3 fatty acids ratio was higher in the four types of HongJams. Most of the minerals were higher in four types of HongJams than in WJ, and three heavy metal were not detected in all 5 types of HongJams. Phytochemicals were also most abundant in WJ, but the difference in the amounts were not severe. And pepsin digestibility was the highest for Kumokjam HongJam and the lowest for Dodam-silkworm HongJam, but the difference was not severe. The nutritional component analysis results of this study suggested that four new varieties can be used for producing HongJams, and Daebaekjam can replace White-Jade the most as the protein source.

• 한방안이비인후피부과학회지
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• 제35권4호
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• pp.47-62
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• 2022

Objectives : To analyze and understand the clear meaning of Nochae(勞瘵), which is used in the same meaning as tuberculosis in Korean medicine. Methods : Nochae was mainly investigated using the Korean medicine classic DB(https://mediclassics.kr). Records that clearly revealed the visual observation of Nochaechung(勞瘵蟲), the cause of Nochae, and those that do not were arranged in chronological order. It was also analyzed what modern diseases are similar to Nochae. Results : Records that Nochaechung can be observed visually through feces or vomiting suggested the possibility of ascariasis. Nochaechung, which has a shape similar to silkworms and causes symptoms such as coughing and hemoptysis similar to those of tuberculosis while parasitic on human, suggested the possibility of paragonimus westermani. Based on the record that it showed symptoms of coughing and hemoptysis along with chills, fever, and weight loss, Nochae might have been a disease such as pneumonia and lung cancer as well as tuberculosis. Records of women's bloating, weight loss, anorexia, and jaundice suggested that Nochae might have been a liver cirrhosis. Conclusions : Records that clearly reveal the visual observations of Nochaechung, suggested that Nochae was a parasitic disease such as ascariasis or paragonimiasis. Records that visual observation of Nochaechung was not clearly revealed, suggested that Nochae might have been diseases such as pneumonia, lung cancer, and liver cirrhosis in addition to tuberculosis.

• 한국응용곤충학회지
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• 제61권2호
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• pp.283-288
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• 2022

본 연구에서는 정서곤충을 이용한 치유프로그램 활동이 지역아동센터를 이용하는 아동 43명의 스트레스에 미치는 영향을 조사하였다. 치유프로그램에 이용된 정서곤충은 누에나방[Bombyx mori (Linnaeus)]이다. 스트레스의 정도는 타액 ɑ-아밀라아제 검사를 통해 측정하였다. 스트레스 수치를 평가하기 위한 타액 샘플 채취와 곤충 선호도에 대한 설문지는 곤충치유활동에 참여하기 전과 후에 측정되었고, 활동을 모두 마친 후에는 치유프로그램 참여 경험에 대한 만족도 점수를 평가하였다. 분석 결과, 곤충치유프로그램 활동은 참여 아동의 타액 ɑ-아밀라아제 수치를 통계적으로 낮추고, 곤충에 대한 선호도를 높여준다는 결과를 확인하였다. 누에를 이용한 치유프로그램 활동에 참가한 아동들의 만족도는 5점 만점에 4.23점이었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.127-131
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• 2002

The qualitative and quantitative changes of bacterial flora associated with the Pure Mysore (Multivoltine) and NB$_4$D$_2$ (Bivoltine) silkworm (Bombyx modi L.) midgut during third, fourth and fifth instars were studied. Larvae reared on mulberry leaves were dissected and their midgut bacterial populations were enumerated through serial dilution technique and after 72 hrs of incubation period at 28 $\pm$ 1$^{\circ}C$, the bacterial population was estimated. The results showed a highest mean value of 15$\times$10/ sup 6/ sup 6/ CFU/g and 28$\times$10/ sup 6/ CFU/g in Pure Mysore and NB$_4$D$_2$races, respectively, in midgut tissue of fifth instar larvae. The natural epiphytic microflora of mulberry leaves fed during the respective instars was also studied and found maximum 14$\times$10$^3$ CFU/g in leaves fed in third instars, followed by 5.3$\times$10$^3$ CFU/g and 2.1$\times$10$^3$ CFU/g in leaves fed during fourth and fifth instars, respectively. The bacterial flora colonized in midgut was found to be elaborating amylase, caseinase, gelatinase, lipase and urease enzymes. The highest percentages of isolates were amylase producers followed by protein and lipid splitters in Pure Mysore, whereas in NB$_4$D$_2$ protein splitter were dominated followed by lipase and amylase producers in NB$_4$D$_2$. The results indicate that the natural microflora may play a vital role in the digestion of ingested food materials in silkworms.