• 한국잠사곤충학회지
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• 제46권2호
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• pp.54-57
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• 2004

다수성 품종으로 육성된 부흥잠은 새로운 가을누에 품종으로서 육성경위와 주요특성을 요약 하면 다음과 같다. 1. 일본종계 원종 잠149와 도입 육성한 무늬 품종이며, 중국종계 원종 잠150은 도입 육성한 강건다수성 한성무늬 누에품종이다. 2. 부흥잠의 일본종계 원종인 잠149은 조사된 주요 양적형질 중 화용비율, 견층비율 및 견사장에서 일반조합능력(GCA)이 높았고, 중국종계 잠150은 견층비율 및 견 사장에서 GCA가 높게 나타났다. 3. 2003년 추잠기 지역적응시험 결과는 부흥잠이 다수견, 다사량의 특성을 지닌 대성 잠에 비해 전견중이 무거워 고치수량이 6% 증수되었다. 4. 조사성적에서는, 견사장은 1,274 m로 대조대비 지수로 3%가 떨어지나, 섬도가 굵고 견사량이 많아 1만두 생사량이 3.68 kg/만두로 대조 대비 지수로 7%높아 다사량의 특성을 나타내었다. 5. 잠종생산능력에 있어서는 일본종계 원종 잠149의 산란수는 525개로 대성잠의 일본종계 원종인 잠125과 같은 수준이었고 중국종계 원종 잠150의 산란수는 510개로 대성잠의 잠126보다 다소 적었으나 산란성은 우수한 편이었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권2호
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• pp.127-133
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• 2001

With an objective of evolving quantitatively and qualitatively superior bivoltine breeds/hybrids of silkworm Bombyx mori L. for tropical conditions, breeding work was initiated in Central Sericultural Research and Training Institute, Mysore during 1992 by utilizing two Japanese hybrids namely BNl8$\times$BCS25 and Shunrei$\times$Shogetsu along with Indian evolved breed, KA. The breed CSR12 which is characterized with sex-limited larval marking and white oval cocoons was evolved from the Japanese hybrid BNl8 ${\times}$ BCS25 by crossing with KA, while the breed CSR6 which is characterized with normal marking (marked larvae) and white dumbbell cocoons was extracted from the Japanese commercial hybrid Shunrei${\times}$Shogetsu through continuous inbreeding coupled with selection. After fixation, these breeds along with other newly evolved breeds were subjected to hybrid study under optimum environmental conditions in the laboratory for expression of full potential of the genotypes. These hybrids were evaluated by Multiple Trait Evaluation Index (Mano et al., 1993). The hybrid CSR12${\times}$CSR6 was selected based multiple trait evaluation index value. The hybrid CSR12$\times$CSR6 recorded survival of 96.0%, shell weight of 50.0 cg, shell ratio of 24.3%, raw silk percentage of 19.6, filament length of 1,216 m, boil off loss of 22.4% and renditta of 5.1. On the other hand, the control hybrid (KA ${\times}$ NB4D2) has recorded survival of 90.6%, shell weight of 42.1 cg, shell ratio of 20.4%, raw silk percentage of 15.9, filament length of 999 m, boil off loss of 24.8% and renditta of 6.3. The hybrid CSR12$\times$CSR6 was authorized during 1997 by Central Silk Board, Government of India for commercial exploitation during favourable months based on national level race authorization test.

• Journal of Microbiology
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• 제45권2호
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• pp.139-145
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• 2007

The Bombyx mori parvo-like virus (China isolate) DNA polymerase (BmDNV-3 dnapol) gene has been tentatively identified based on the presence of conserved motifs. In the present study, we perform a transcriptional analysis of the BmDNV-3 dnapol gene using the total RNA isolated from BmDNV-3 infected silkworm at different times. Northern blot analysis with a BmDNV-3 dnapol-specific riboprobe showed a major transcript of 3.3 kb. 5'-RACE revealed that the major transcription start point was located 20 nucleotides downstream of the TATA box. In a temporal expression analysis using differential RT-PCR, BmDNV-3 dnapol transcript was detected at low levels at 6 h.p.i., increased from 6 to 36 h.p.i., and remained fairly constant thereafter. Analysis of the predicted DNA polymerase sequence using neighborjoining and protein parsimony algorithms indicated that the predicted 1115-residue polypeptide contained five motifs associated with DNA polymerases synthetic activities and three additional motifs associated with polymerases possessing 3' to 5' exonuclease activity. The molecular phylogenetic analysis of this gene supported the placement of Bombyx mori parvo-like virus in a separate virus family.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권1호
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• pp.1-5
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• 2008

The present study was carried out with the aim to evaluate the viability of layings (delivery of fertilized and unfertilized eggs) with egg laying duration of elite bivoltine races. The temporal aspect of mating in terms of egg layings duration may also have impact on the number of eggs laid, pattern of egg laying and their viability. After different interval of egg laying duration, moth of Bombyx mori are removed from oviposition site after they have completed egg laying. Present investigation confirmed that there are no significant difference in relation to the occurrence of viable (fertilized) and non viable (unfertilized) eggs and duration of oviposition. Percentage of unfertilized eggs varied from 2.41 to 3.42% in case of CSR-3, 2.59 to 3.62% in case of CSR-6, 2.82 to 3.66% in case of CSR-16 and 2.58 to 3.40% in case of CSR-17 in different treatments. The occurrence of unfertilized eggs are also not significant in the different treatments specially in those eggs which were laid 24 hours after oviposition and kept for 4 months hibernation schedule.

• 한국잠사곤충학회지
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• 제49권1호
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• pp.14-17
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• 2007

다수성 품종으로 육성된 골든실크는 새로운 봄누에 특수용도 품종으로서 육성경위와 주요특성을 하면 다음과 같다. 1. 일본종계 원종 잠311은(견색적$\times$흑아)$\times$잠125 교잡 육성한 무늬 품종으로 황색고치를 짓고, 중국종계 원종 잠312는 잠304$\times$괴축과 교잡 육성한 무늬 없는 누에품종으로 황색고치를 짓는 품종이다. 2. 2006년 춘잠기 지역적응시험 결과는 골든실크의 주요특성은 - 실용 부화비율은 97%, 화용비율 및 상견비율은 대조품종보다 우수하였다. 수량성 등 다른 조사항목에서는 대조보다 좋지 못하였으나, 전령기간은 1일 12시간이 짧아 사육노동력을 줄일 수 있는 이점이 있다. - 견사장은 1,031m였고, 견사섬도는 2.97 데니어 이었다. - DNJ 함량은 대조 춘수잠 3.81 mg보다 14% 높은 4.34mg으로 우수 하였고, 동충하초 생산력 검정에서는 동충하초 생체중이 1개당 대조 1.41g보다 낮은 1.20g이였다. 3. 잠종생산능력에 있어서는 일본종계 원종 잠311의 나방당 산란수는 604개로 대조보다 많았고, 최청에서 발아까지의 일수는 54일 이었다. 중국종계 원종 잠312의 산란수는 493개로 일반장려품종의 원종과 산란성이 비슷하였고, 최청에서 발아까지의 일수는 52일이었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.123-127
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• 2001

In order to introduce bivoltine races in a tropical country like India, it is necessary to have stability in cocoon crop under high temperature environments. Unlike any temperate country like Japan, the rearing conditions/environment, climatic conditions, quality of mulberry leaf and incidence of diseases are unpredictable in India. Geneticists and breeders of all the sericultural countries have experienced the influence of environment during the process of breeding. in order to select efficiently the breeds with high temperature tolerance, it is verb important to analyse clearly the heritability nature of high temperature tolerance. In light of the above, the present study was undertaken to determine the effect of high temperature treatment of (A) $35{\pm}1^{\circ}C$ and 85${\pm}$5% RH for 24 hrs continuously, (B) $35{\pm}1^{\circ}C$ and 85${\pm}$5% RH for 48 hrs continuously and (C) the control (25${\pm}1^{\circ}$ and 65${\pm}$5% RH in the normal rearing condition from the $3^{rd}of5^{th}$ instar on the pure races such as Moria, Nl37 and Cl46 as well as their Fl hybrids. The overall performance indicate that the hybrids are mare tolerant than the pure races. it was also observed that the overall performance declined in those batches where 48 hrs treatment was given. The most interesting observation noticed in this study was that there was maternal effect regarding temperature tolerance as evident from the better performance of those hybrids where the female parent used was more tolerant as pure race.