Browse > Article

Transcriptional Analysis of the DNA Polymerase Gene of Bombyx mori Parvo-like Virus (China Isolate)  

Wang, Yong-Jie (Institute of Fisheries, Anhui Academy of Agricultural Sciences)
Chen, Ke-Ping (Institute of Life Sciences, Jiangsu University)
Yao, Qin (Institute of Life Sciences, Jiangsu University)
Han, Xu (Institute of Life Sciences, Jiangsu University)
Publication Information
Journal of Microbiology / v.45, no.2, 2007 , pp. 139-145 More about this Journal
Abstract
The Bombyx mori parvo-like virus (China isolate) DNA polymerase (BmDNV-3 dnapol) gene has been tentatively identified based on the presence of conserved motifs. In the present study, we perform a transcriptional analysis of the BmDNV-3 dnapol gene using the total RNA isolated from BmDNV-3 infected silkworm at different times. Northern blot analysis with a BmDNV-3 dnapol-specific riboprobe showed a major transcript of 3.3 kb. 5'-RACE revealed that the major transcription start point was located 20 nucleotides downstream of the TATA box. In a temporal expression analysis using differential RT-PCR, BmDNV-3 dnapol transcript was detected at low levels at 6 h.p.i., increased from 6 to 36 h.p.i., and remained fairly constant thereafter. Analysis of the predicted DNA polymerase sequence using neighborjoining and protein parsimony algorithms indicated that the predicted 1115-residue polypeptide contained five motifs associated with DNA polymerases synthetic activities and three additional motifs associated with polymerases possessing 3' to 5' exonuclease activity. The molecular phylogenetic analysis of this gene supported the placement of Bombyx mori parvo-like virus in a separate virus family.
Keywords
Bombyx mori parvo-like virus; DNA polymerase gene; conserved motif; transcription analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Hess, M., H. Blocker, and P. Brandt. 1997. The complete nucleotide sequence of the egg drop syndrome virus: An intermediate between mastadenoviruses and aviadenoviruses. Virology 238, 145-1565   DOI   ScienceOn
2 Wang, Y.J., Q. Yao, K.P. Chen, and X. Han. 2006. Organization and transcription strategy of genome of Bombyx mori bidensovirus (China isolate) $VD_{1}$. Chinese J. Biotechnol. 22, 706-712
3 Bensimhon, M.J., J. Gabaeeo-Arpa, and R. Ehrlich. 1983. Physical characteristics in eukaryotic promoters. Nucleic Acids Res. 11, 4521-4540   DOI
4 Bernard, A., J.M. Lazaro, M. Salas, and L. Blanco. 1990. The highy conserved amino acid sequence motif Tyr-Gly-Asp-Thr- Asp-Ser on alike DNA polymerase is required by a phase f29 DNA polymerase for protein-primed initiation and polymerization. Proc. Natl. Acad. Sci. USA 87, 4610-4614
5 Blanco, L. and M. Salas. 1996. Relating structure to function in phi29 DNA polymerase. J. Biol. Chem. 271, 8509-8512   DOI   ScienceOn
6 Bulach, D.M., A. Kumar, A. Zaia, L. Bufeng, and D.E. Tribe. 1999. Group II nucleopolyhedrovirus subgroups revealed by a phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J. Invertebr. Pathol. 73, 59-73   DOI   ScienceOn
7 Court, D.A. and H. Bertrand. 1992. Genetic organization and structural features of maranhar, a senescence-inducing linear mitochondrial plasmid of Neurospora crassa. Curr. Genet. 22, 385-397   DOI
8 Tomalski, M.D., J.G. Wu, and L.K. Miller, 1988. The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology 167, 591-600   DOI   PUBMED
9 Kornberg, A. and T.A. Baker. 1991. DNA Replication, 2nd (ed.), In W.H. Freeman, New York, USA
10 Lo, C.F., H.C. Hsu, M.F. Tsai, C.H. Ho, S.E. Peng, G.H. Kou, and D.V. Lightner. 1999. Specific genomic fragment analysis of different geographical clinical samples of shrimp white spot syndrome virus. Dis. Aquat. Org. 35, 175-185   DOI
11 Chan, B.S., D.A. Court, P.J. Vierula, and H. Bertrand. 1991. The kalilo linear senescence-inducing plasmid of Neurospora is an invertron and encodes DNA and RNA polymerases. Curr. Genet. 20, 225-237   DOI
12 Lee, A.M., R.G. Ivey, and R.H. Meints. 1998. Repetitive DNA insertion in a protein kinase ORF of a latent FSV (Feldmannia sp. virus) genome. Virology 248, 35-45   DOI   ScienceOn
13 Vladimir, V. and J.J. Kapitonov. 2006. Self-synthesizing DNA transposons in eukaryotes. PNAS. 103, 4540-4545   DOI   ScienceOn
14 Grabherr, R., P. Strasser, and J.L. Van Etten. 1992. The DNA polymerase gene from chlorella viruses PBCV-1 and NY-2A contains an intron with nuclear splicing sequences. J. Virol. 188, 721-7315   DOI   ScienceOn
15 Nakai, R., K. Sen, S. Kurosawa, and H. Shibai. 2000. Basidiomycetous fungus Flammulina velutipes harbors two linear mitochondrial plasmids encoding DNA and RNA polymerases. FEMS Microbiol. Lett. 190, 99-102   DOI
16 Klassen, R. and F. Meinhardt. 2003. Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora. Mol. Genet. Genomics 270, 190-199   DOI
17 Liu, J.J. and E.B. Carstens. 1995. Identification, localization, transcription, and sequence analyses of the Choristoneura fumiferana nuclear polyhedrosis virus DNA polymerase gene. Virology 209, 538-549   DOI   ScienceOn
18 Lu, H.S. 1998. Molecule biology of insect virus. China agriculture science and technology publishing company press, p. 382-391
19 Morrison, A., J.B. Bell, T.A. Kunkel, and A. Sugino. 1991. Eukaryotic DNA polymerase aminoacid sequence required for 3'$\rightarrow$5' exonuclease activity. Proc. Natl. Acad. Sci. USA 88, 9473-9478
20 Ishino, M., Y. Sawada, T. Yaegashi, M. Demura, and K. Fujinaga. 1987. Nucleotide sequence of the adenovirus type 40 inverted terminal repeat: Close relation to that of adenovirus type 5. Virology 156, 414-416   DOI   ScienceOn
21 Kozak, M. 1986. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 44, 283-292   DOI   ScienceOn
22 Hermanns, J. and H.D. Osiewacz. 1992. The linear mitochondrial plasmid pAL2-1 of a long-lived Podospora anserina mutant is an invertron encoding a DNA and RNA polymerase. Curr. Genet. 22, 491-500   DOI
23 Lu, A., P.J. Krell, J.M. Vlak, and G.F. Rohrmann. 1997. Baculoviruses DNA Replication. p. 171-191. In Miller, L.K. (ed.), The Baculoviruses. Plenum Press, New York, USA
24 Wang, T.S.F. 1991. Eukaryotic DNA polymerases. Annu. Rev. Biochem. 60, 513-552   DOI   ScienceOn
25 Handa, H., K. Itani, and H. Sato. 2002. Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.). Mol. Genet. Genomics 267, 797-805   DOI
26 Bois, F., G. Barroso, P. Gonzalez, and J. Labarere. 1999. Molecular cloning, sequence and expression of Aa-polB, a mitochondrial gene encoding a family B DNA polymerase from the edible basidiomycete Agrocybe aegerita. Mol. Gen. Genet. 261, 508-513   DOI
27 Chen, L.L., J.H. Leu, C.J. Huang, C.M. Chou, S.M. Chen, C.H. Wang, C.F. Lo, and G.H. Kou. 2002. Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting vp35 to the nuclei of transfected insect cells. Virology 293, 44-53   DOI   ScienceOn
28 Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882   DOI
29 Swofford, D.L. 1998. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0 Sinauer, Sunderland, MA
30 Verheust, C., N. Fornelos, and J. Mahillon. 2005. GIL16, a New Grampositive tectiviral phage related to the Bacillus thuringiensis GIL01 and the Bacillus cereus pBClin15 elements. J. Bacteriol. 187, 1966-1973   과학기술학회마을
31 Kim, E.K., J.H. Jeong, H.S. Youn, Y.B. Koo, and J.H. Roe. 2000. The terminal protein of a linear mitochondrial plasmid is encoded in the N-terminus of the DNA polymerase gene in white-rot fungus Pleurotus ostreatus. Curr. Genet. 38, 283-290   DOI