• 한국식품위생안전성학회지
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• 제11권2호
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• pp.123-127
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• 1996

The isolation and chemical characterization of yellow food pigments from Monascus purpureus were studied according to the compositions of media. Monacus yellow pigments were isolated and purified by solvent fractionation, silicagel column chromatography, TLC and HPLC. The retention time of Monascus yellow pigments isolated by HPLC was respectively 5 min(I) and 9 min(II) as the yeast malt extract agar(YMA) media and was respectively 4.6 min(III), 5 min(I) 5.7 min(IV), 8.3 min(V), 9 min(II) and 10.7 min(Ⅵ) at the malt extract agar(MEA) media. The structure of monascin(I), ankaflavin(II), 6,11-dihydrorubropunctatin(III), 6,11-dihydromonascorubrin(V) and unknown compounds(IV,Ⅵ) was elucidated by EI-Mass, H and C NMR, UV-visible spectrometer. Therefore, it was suggested that 6,11-dihydrorubropunctatin(III) and 6,11-dihydromonascorubrin(V) are new intermediates of Monascus yellow pigments.

• 한국수소및신에너지학회논문집
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• 제1권1호
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• pp.9-14
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• 1989

Mixed photocatalyst containing cadmium sulfide and zinc sulfide was prepared on silica gel powder and Nafion film. Photo-irradiation of aqueous mixture containing the photocatalysis generated hydrogen by water cleavage reaction. Use of sodium sulfide as sacrificial reagent help the photo-reaction. Evolution of the hydrogen was measured by gas chromatographic analysis. Composition of the catalyst was determined by atomic absorption spectrophotometer. 0.2 mL of of hydrogen was generated per hour. The maximun catalytic activity was obtained after 8-12 hours later. Hydrogen generation efficiency by the two different catalytic system was compared and showed that the Nafion-based catalyst is more efficient than the silicagel-based catalyst for the photoreaction.

• 생약학회지
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• 제30권3호
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• pp.332-334
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• 1999

Corydalis Tuber has been used in traditional medicine for an analgesic, antispasmodic and gastric ulcers. For the quality control on this drug, isolation and quantitative determination of berberine and coptisine from Corydalis ternata Nakai (Papaveraceae) has been conducted by using HPLC method. Berberine and coptisine in quarternary alkaloidal fraction from the crude drug were separated on silicagel column using a $CHCl_3:MeOH\;(85:15)$ and $CHCl_3:MeOH:H_2O\;(70:30:4)$ as an eluent, and the average contents were about 0.93 and 0.36%.

• 한국식품위생안전성학회지
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• 제12권3호
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• pp.195-199
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• 1997

For the purpose of isolation and screening of tyrosinase inhibitory activity from edible mushrooms, Pleurotus ostreatus, Auricularia auricula-Judae, Umbilicaria esculenta, Agaricus bisporus, Flammuline velutipes, Lentinus edodes, Ganoderma lucidum, and Coriouls versicolor were examined by tracing inhibitory activities against tyrosinase, utilizing L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate. Among the eight edible mushrooms tested, Umbilicaria esculenta showed potent enzyme inhibitory activities above 7804% against tyrosinase in ethylacetate (EtOAc) extracts. Ganoderma lucidum and Agaricus bisporus showed inhibitory activities of 67.3% and 51.5% in water extracts. EtOAc extracts of Umbilicaria esculenta was fractionated from silicagel column chromatography and one fraction showed the most inhibitory activity of 60.9%. The three bands (Rf=0.38, 0.27, 0.19) were isolated from preparative TLC of the fraction for purification and identified as mixtures of orsellinate, methyl orsellinate, methyl lecanorate, and methyl gyrophorate by high pressure liquid chromatography (HPLC), ultravisible spectrophotometer (UV), mass spectrophotometer (Mass), nuclear magnetic resonance spectrometer (NMR).

• 한국대기환경학회지
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• 제18권6호
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• pp.475-485
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• 2002

An analytical method was investigated for the meaiiurement of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) concentrations in air samples. Procedures required for column chromatographic clean up. silicagel (stage I) and gel permeation chromatography (stage II), were discussed. Identification and quantification of PCBs and OCPs were performed using a combination of gas chromatography/mass spectrometry/ selected ion monitoring. Recovery tests calculated from six samples are 68∼137% for PCBs and 58∼130% for OCPs except for endrin aldehyde. Instrumental detection limits determined for the PCBs and OCPs varied from 0.05 to 0.18 pg/m3 and from 0.71 to 16.82 pg/㎥, respectively. The method has been applied to the analysis of air samples collected at Ansung city, Kyonggi province, Korea. This method may serve as a screening protocol for the simultaneous determination of PCBs and OCPs in air.

• 약학회지
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• 제33권3호
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• pp.175-182
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• 1989

To find ${\alpha}-amylase$ inhibitors produced by microorganisms from soil, a strain which had a strong inhibitory activity against bacterial ${\alpha}-amylase$ was isolated from the soil sample collected in Korea. The morphological and physiological characteristics of this strain on several media and its utilization of carbon sources showed that it was one of Streptomyces species according to the International Streptomyces Project method. The amylase inhibitor of this strain was purified by active carbon adsorption, silicagel column chromatography, SP-Sephadex C-25 column chromatography, adsorption on Amberlite XAD-2. The inhibitor was oligosaccharide which was composed of glucose. The inhibitor had inhibitory activity against other amylase such as salivary ${\alpha}-amylase$, pancreatic ${\alpha}-amylase$, fungal ${\alpha}-amylase$ and gluco-amylase.

• 약학회지
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• 제40권1호
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• pp.46-51
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• 1996

Eight new D-1.3-dioxolanyl and L-1,3-oxathiolanyl nucleosides containing 5-phenyl- selenyl pyrimidine bases which are expected to have antiviral activity were synthesized. Condensation of D-1,3-dioxolane acetate and L-1,3-oxathiolane acetate with 5-phenylselenyl pyrimidines gave anomeric mixtures of their nucleosides which were separated by silicagel column chromatography.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.334.2-334.2
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• 2002

As the part of our continuing study for antihyperlipidemic agents from the herbal medicinal resources. we examined the possibility of the ethylacetate fraction of Alpinia officinarum water extract in vitro and in vivo. We isolated some compounds from the ethylacetate fraction of Alpinia officinarum and measured their antihyperlipidemic activities. The active components isolated by silicagel column lmproved serum TG. HDL and LDL level in corn oil feeding and triton WR-1339 induced hyperlipidemic mice. The most active compound was 3-Methylethergalangin. Consequently. these biologically active gerbal functional foods could be used for preparing the healthy food which might diabetas. hyperlipidemia and other disease.

• 한국미생물·생명공학회지
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• 제33권2호
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• pp.123-129
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• 2005

참비름(Amaranthus lividus)의 에탄올 추출물이 6종의 분리 병원성 미생물에 대해 항 미생물 활성을 나타내었으며 silicagel column chromatography에서는 8개의 fraction중에서 7번째가 6개의 분리 미생물에 대해 clear zone을 형성하여 가장 높은 항균력을 나타내었고 특히 A. sobria CLFM1 은 31mm, S. spp.는 33mm로 가장 큰 clear zone을 형성하였다. 활성물질의 순도를 확인하고자 methanol에 용해시킨 시료를 n-hexane : Ethyl acetate(1:1, v/v)용매계로 TLC를 전개시킨 결과 Rf 13.3의 위치에서 단일 spot를 나타내어 활성물질이 대단히 정제되어 있었으며, HPLC로 확인 결과 retention time 3.36에 single peak를 나타내 단일 물질임을 확인할 수 있었다. 분리된 활성물질을 GC-MS(m/z)로 분석한 결과 m/z 222에서 base peak로 나타났으며 이 spectrum으로 NIST library 검색을 실시 한 결과, $C_{12}H_{14}O_4$의 diethyl phtalate로 시사되었다. C-NMR과 1H-NMR을 실시한 결과 참비름에서 분리한 물질은 구조식 C12H14O6인 diethyl phtalate로 동정되었다. 참비름(Amaranthus lividus)의 에탄올 추출물이 6종의 분리 병원성 미생물에 대해 항 미생물 활성을 나타내었으며 silicagel column chromatography에서는 8개의 fraction중에서 7번째가 6개의 분리 미생물에 대해 clear zone을 형성하여 가장 높은 항균력을 나타내었고 특히 A. sobria CLFM1은 31 mm, S. spp.는 33 mm로 가장 큰 clear zone을 형성하였다. 활성물질의 순도를 확인하고자 methanol에 용해시킨 시료를 n-hexane : Ethyl acetate(1:1, v/v)용매계로 TLC를 전개시킨 결과 Rf 13.3의 위치에서 단일 spot를 나타내어 활성물질이 대단히 정제되어 있었으며, HPLC로 확인 결과 retention time 3.36에 single peak를 나타내 단일 물질임을 확인할 수 있었다. 분리된 활성물질을 GC-MS(m/z)로 분석한 결과 m/z 222에서 base peak로 나타났으며 이 spectrum으로 NIST library 검색을 실시 한 결과, $C_{12}H_{14}O_4$의 diethyl phtalate로 시사되었다. C-NMR과 1H-NMR을 실시한 결과 참비름에서 분리한 물질은 구조식 $C_{12}H_{14}O_6$인 diethyl phtalate로 동정되었다.

• 한국식품과학회지
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• 제30권1호
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• pp.230-236
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• 1998

충치 원인균인 S. mutans가 생산하는 불용성, 부착성 glucan 합성효소인 GTase의 활성을 저해하는 물질을 생약재로 부터 탐색, 개발하여 기능성 식품 또는 의약품의 소재로 응용하는 것을 목적으로 연구를 수행하였다. 32종의 생약재 추출물에 대하여 GTase 저해활성을 측정한 결과, 후박피에서 가장 높은 활성이 관찰되었다. 후박피 추출물에 대하여 용매분획을 행한 결과 ethylacetate층에 대부분의 활성이 존재하였으므로, 이 획분을 silicagel column chromatography, prep. HPLC 등을 이용하여 더욱 분리, 정제하여 순수한 GTase 저해물질을 분리하였다. 이 물질의 분리수율은 0.013% (w/w)였다. 이 화합물을 UV, FAB-MS 및 NMR spectrometry 등을 이용하여 화학구조의 해석을 실시한 결과 이 물질은 coniferyl alcohol의 중합체로 lignan계 화합물인 4,4'-dihydroxy-3,3'-dimethoxylignan (분자량 330)으로 확인되었다. 이 화합물은 후박피에서는 최초로 확인된 물질이다. 또한 충치 원인균인 S. mutans를 포함한 11종의 구강 미생물에 대한 항균성을 검토한 결과 비교 화합물에 비해 비교적 강력한 활성$(MIC;\;31.3\;{\mu}g/ml)$이 나타났다. 지금까지 후박피는 반하후박탕, 후박삼물탕 등 한방약재의 중요한 원료로 이용되어 왔으며, 복통의 치료효과 및 건위 작용 등이 알려져 있다. 또한 후박피 중에는 diphenyl계 화합물인 magnolol 및 honokiol등이 함유되어 있어 이들 물질이 건위작용을 돕는 것으로 알려져 있을 뿐이다. 따라서 이번에 새롭게 확인된 4,4'-dihydroxy-3,3'-dimethoxylignan이 충치 예방효과를 지니고 있다는 사실은 금후 산업적 응용면에서도 매우 중요한 결과로 여겨져 지속적인 연구가 기대된다.