• 한국식품영양과학회지
• /
• 제30권4호
• /
• pp.732-738
• /
• 2001

두충잎에서 인간대장암에세포 HCT-116에 세포증식 억제효과를 나타내는 성분을 silica gel column chromatography TLC로 분리하고 HPLC로 정제한 다음 UV-visual spectro-photometer에 의한 흡수 spectra 특성 HPLC에 의한 retention time과 분리패턴 및 FAB/MS로 분자량을 잠정적으로 동정한 결과 TLC 상에서 분리된 Rf 0.19 band 의 화합물은 chlorophyll a에서 $Mg^{2+}$이 제거된 pheophytin a로 Rf 0.25 band의 화합물은 pyropheophytin a로 확인되었다. 동정된 화합물들의 암세포 억제작용을 확인하기 위해 두충잎의 petroleum ether extract column chromatography로 분리한 분획물 및 TLC에서 분리된 각 band들의 cytotoxic activity을 in vitro에서 HCT-116 cancer cell을 사용하여 측정한 결과 모든 시료에서 암세포 증식억제 현상을 보였으며 특히 Rf 0.19와 Rf 0.25 band에서 분리된 화합물들에서 강하게 나타났다. 이 결과로 볼 때 두충잎에서 분리한 클로로필 유도체는 인간대장암세포인 HCT-116 cell에 대해 강한 항암작용이 있는 것으로 생각된다.

• 한국식품영양학회지
• /
• 제12권5호
• /
• pp.508-512
• /
• 1999

In order to qualify and quantify carotenoid pigment of pumpukin were analyzed by HPLC column chromatography and spectrophotometry. Eightyseven percent of total carotenoids in the pumpkin was in the fiber amounting 65.33mg%. however flesh and skin contained 6.61 and 3.31mg% respectvely. A silica gel column chromatography of the carotenoids produced 2 different peaks. The components eluted in peak II showed blue color when treated with hydrochloric acid. The fact suggested it contained epoxide and the component in peak II was a monol. By the separation with HPLC. $\alpha$ and $\beta$-carotene were the major compounds in the carotenoids. In fiber the total $\beta$-carotene content was 1.6 times higher than in the flesh and skin. The fiber contained less unidentified compounds than other part which contained 27.3~32.8%.

• 약학회지
• /
• 제40권4호
• /
• pp.462-467
• /
• 1996

Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active consituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions(fr. 1-5). The active constituents I and II were isolated from fr.2 and 3, respectively, by repeated silica gel column chromatography and recrystallization. The constituents were identified as 3${\beta}$-acetylbetulinic acid and betulinic acid by means of physicochemical data. The $ED_{50}$ values of 3${\beta}$-acetylbetulinic acid and betulinic acid were 9.10 and 16.43${\mu}g$/ml against L1210 cells and 2.72 and 2.41${\mu}g$/ml against HL60 cells, respectively.

• Archives of Pharmacal Research
• /
• 제25권3호
• /
• pp.336-342
• /
• 2002

MeOH extract of Kochia scoparia was fractionated into $CHCl_3-$, EtOAc- and BuOH extracts and the last fraction were hydrolyzed by 3%-NaOH ($MeOH-H_2O$) to compare the bioactivities on antinociceptive and anti-inflammatory effects. Silica gel column chromatography of BuOH fraction afforded a large amount of $3-Ο-{\beta}-D-xylopyranosyl {\;}(1{\rightarrow}3)-{\beta}-D-glucuronopyranosyl$ oleanolic acid (momordin Ic, 4) and that of acid hydrolysate of BuOH fraction gave $3-Ο-{\beta}-D-glucuronopyranosyl oleanolic$ acid (momordin Ib, 3), its 6'-Ο-methyl ester (2) and oleanolic acid (1). Silica gel column chromatography of alkaline hydrolysate afforded a large amount of 4. MeOH extract and both EtOAc- and BuOH fractions were active in the rheumatoidal rat induced Freund's complete adjuvant reagent (FCA) whereas $CHCl_3$ fraction was inactive. Compound 1 and 4 showed significant activities in the same assay but oleanolic acid 3-Ο-glucuronopyranoside (3) showed no activity. These fashions were also observed in carrageenan-induced edema of the rat and in the antinociceptive activity tests undertaken in hot plate- and writhing methods. These results suggest that momordin Ic and its aglycone, oleanolic acid, could be active principles for rheumatoid arthritis.

• 한국잡초학회지
• /
• 제30권4호
• /
• pp.437-444
• /
• 2010

본 연구는 진노랑상사화 인경에 함유된 allelochemical의 살초활성을 조사하고, 활성 발현에 관련된 살초 활성 물질을 분리하여 화학구조를 밝히고자 수행되었다. Silica gel flash column chromatography, HPLC 등 일련의 크로마토그래피법들을 통해 진노랑상사화 인경의 MeOH 추출물을 분리 정제하였다. HPLC에 의해 최종 획득된 활성분획에 대한 GC/MS 분석결과, isoquinoline alkaloid인 montanine($O^2$-methyl pancracine)이 동정되었다. Montanine은 $50{\mu}g\;mL^{-1}$ 농도에서 돌피 유묘의 생장을 100% 억제시키는 것으로 나타났다.

• Applied Biological Chemistry
• /
• 제32권2호
• /
• pp.137-142
• /
• 1989

인삼 에칠아세테이트 추출 분획을 silica gel column chromatography하여 Fr. I, Fr. II 및 Fr. III으로 나눈 다음 항암제 투여로 조혈기능이 억제된 흰쥐에 투여해 본 결과 Fr. III이 감소된 백혈구 및 적혈구 수를 90%까지 회복시켰다. Fr. III에서 두성분을 분리하여 구조를 동정한 결과 ginsenoside $Rh_1$ 및 $Rg_2$로 확인하였다.

• 대한화학회지
• /
• 제39권7호
• /
• pp.524-529
• /
• 1995

pH 1에서 15-crown-5(SGBM)을 결합시킨 silica gel를 고정상으로 하는 액체 크로마토그래피을 이용하여 혼합 금속이온 용액으로부터 금속이온들의 분리효율을 결정하였다. 여러가지 SGBM-금속이온들간의 결합상수 및 분리인자들을 측정한 결과 그 순위는 Li(Ⅰ) < Sr(Ⅱ) < Na(Ⅰ) < Cu(Ⅱ) < Mg(Ⅱ) < K(Ⅰ) < Ca(Ⅱ) < Bi(Ⅲ) < Sb(Ⅲ)로 나타났다. 이러한 사실은 금속이온의 크기 효과 및 전자 밀도 효과에 의해서 결정됨을 알 수 있었으며, 또한 알칼리, 알칼리 토금속이온 및 $Cu^{2+}$이 혼합된 용액에서 $Sb^{3+}$의 분리효율이 다른 이온들에 비해서 훨씬 좋게 나타남을 알았다.

• 한국식품영양학회지
• /
• 제21권2호
• /
• pp.143-147
• /
• 2008

To develop a new anti-wrinkle agent from medicinal plants, this study investigated the optimal conditions for extracting elastase inhibitor from Rheum undulatum L. Maximal extraction occurred by using 70% methanol at $50^{\circ}C$ for 24 hr; the elastase inhibitory activity was 60.4%($IC_{50}:6.7{\times}10^3{\mu}g/m{\ell}$). Systematic solvent extraction, thin layer chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and reverse-phase high performance liquid chromatography were used in the partial purification of the elastase inhibitor. The compound was soluble in dimethylsulfoxide, methanol, and ethanol, and had maximum absorption spectra at 231.5 nm and 275.5 nm.

• Journal of the Korean Wood Science and Technology
• /
• 제19권3호
• /
• pp.72-76
• /
• 1991

The new preparative separation techneque of dihydroquercetin (2, 3-trans-dihdroxy-3, 3', 4', 5, 7-pentahydroxydihydroflavonol) was investigated by liquid chromatography. Also some typical coniferous wood bark were examined for the sources of dihydroquercetin. The good sources of dihydroquercetin were Douglas-fir[Pseudotsuga menziesii] bark and pitch pine [Pinus rigida] bark. There is no dependance the produced place and species dihyroquercetin which is separated by silica-gel column chromatography was taken with white needle-like crystals. This crystals were very stable in the humidity and on the light. It also can stored very long without derivatives.

• 한국식품영양학회지
• /
• 제13권2호
• /
• pp.146-151
• /
• 2000

To evaluate anticaries and antiinflammation of Aloe vera peel, antimicrobial substances were extracted from Aloe vera peel and identified. The antimicrobial active substances of water extract were successfully purified with solvent fractionation, silica gel column chromatography, preparative thin layer chromatography and UV spectrophotometer. Two purified active substances were identified as aloe-emodin and barbaloin by Mass Spectrometer, 1H-NMR and FT-IR.