• Molecules and Cells
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• 제38권5호
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• pp.441-451
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• 2015

Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.

• BMB Reports
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• 제43권5호
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• pp.311-318
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• 2010

This review explored the mechanism of breast carcinoma progression by focusing on integrins and receptor tyrosine kinases (or growth factor receptors). While the primary role of integrins was previously thought to be solely as mediators of adhesive interactions between cells and extracellular matrices, it is now believed that integrins also regulate signaling pathways that control cancer cell growth, survival, and invasion. A large body of evidence suggests that the cooperation between integrin and receptor tyrosine kinase signaling regulates certain signaling functions that are important for cancer progression. Recent developments on the crosstalk between integrins and receptor tyrosine kinases, and its implication in mammary tumor progression, are discussed.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1418-1419
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• 2007

In order to check the electric field distribution of polymer line post insulator, we performed electric field simulation according to state of water drops. Sea water is used to the contaminant. Electric field analysis is performed with change of contact angle, number and distance of water drops.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.358-362
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• 2004

Verification and testing are complementary techniques that are used to increase the level of confidence in the correct functioning of communication systems as prescribed by their specifications. This paper presents an experience of model checking for a formal railway signaling protocol specified in LTS (Labeled Transition System). This formal approach checks deadlock, livelock and reachability for the state and action to verify whether properties expressed in modal logic are true on specifications. We also propose a formal method for semi-automated test case generation for a railway signaling protocol described in I/O FSM (Input/Output Finite State Machine). This enables the generation of more complete and consistent test sequence for conformance testing. The above functions are implemented by C++ language and included within RSPVTE (Railway Signaling Protocol Verification and Testing Environment).

• Journal of Communications and Networks
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• 제4권2호
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• pp.97-101
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• 2002

Based on their shift and scale orthogonality properties, scaling and wavelet functions may be used as signaling functions having good frequency localization as determined by the fractional-out-of-band power (FOOBP). In this paper, application of Daubechies' wavelet and scaling functions as baseband signaling functions is described, with a focus on finding discretely realizable pulse-shaping transfer function circuits whose outputs approximate scaling and wavelet functions when driven by more conventional digital signaling waveforms. It is also shown that the inter-symbol interference (ISI) introduced by the approximation has negligible effect on the performance in terms of signal-to-noise ratio (SNR). Moreover, the approximations are often more bandwidth efficient than the original wavelet functions. These waveforms thus illustrate an example solution of a tradeoff between residual ISI and bandwidth efficiency as a signal design problem.

• BMB Reports
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• 제39권4호
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• pp.406-411
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• 2006

Signaling lymphocyte activation molecule (SLAM; also known as CD150) is a newly identified cellular receptor for measles virus (MV). The interaction between MV Haemagglutin (MVH) and SLAM is an initial step for MV entry. We have identified several novel SLAM binding sites at residues S429, T436 and H437 of MVH protein and MVH mutants in these residues dramatically decrease the ability to interaction with the cell surface SLAM and fail to co-precipitation with SLAM in vivo as well as malfunction in syncytium formation. At the same time, K58, S59 and H61 of SLAM was also identified to be critical for MVH and SLAM binding. Further, these residues may be useful targets for the development of measles therapy.

• Experimental and Molecular Medicine
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• 제50권12호
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• pp.3.1-3.14
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• 2018

Type 1 diabetes mellitus (T1DM) is a pathological condition associated with osteopenia. $WNT/{\beta}$-catenin signaling is implicated in this process. Trabecular and cortical bone respond differently to $WNT/{\beta}$-catenin signaling in healthy mice. We investigated whether this signaling has different effects on trabecular and cortical bone in T1DM. We first established a streptozotocin-induced T1DM mouse model and then constitutively activated ${\beta}$-catenin in osteoblasts in the setting of T1DM (T1-CA). The extent of bone loss was greater in trabecular bone than that in cortical bone in T1DM mice, and this difference was consistent with the reduction in the expression of ${\beta}$-catenin signaling in the two bone compartments. Further experiments demonstrated that in T1DM mice, trabecular bone showed lower levels of insulin-like growth factor-1 receptor (IGF-1R) than the levels in cortical bone, leading to lower $WNT/{\beta}$-catenin signaling activity through the inhibition of the IGF-1R/Akt/glycogen synthase kinase $3{\beta}$ ($GSK3{\beta}$) pathway. After ${\beta}$-catenin was activated in T1-CA mice, the bone mass and bone strength increased to substantially greater extents in trabecular bone than those in cortical bone. In addition, the cortical bone of the T1-CA mice displayed an unexpected increase in bone porosity, with increased bone resorption. The downregulated expression of WNT16 might be responsible for these cortical bone changes. In conclusion, we found that although the activation of $WNT/{\beta}$-catenin signaling increased the trabecular bone mass and bone strength in T1DM mice, it also increased the cortical bone porosity, impairing the bone strength. These findings should be considered in the future treatment of T1DM-related osteopenia.

• 한국철도학회논문집
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• 제6권2호
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• pp.135-141
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• 2003

According to the computerization of railway signaling systems, the interface link between these signaling equipment is also replaced by digital communication channel, expecially communication link for interface between EIS and LDTS, but there are some problems in the present state of railway signaling. First of all, different communication protocol is applied to interface between above two signaling equipment although they have same functions. The other is that the communication protocol currently used in railway field has some unreasonable points such as structure, formation of byte, error correction scheme and etc. To solve these problems, the standard communication protocol for railway signaling is designed. The structure of designed communication protocol and the results of performance analyses are represented in this paper. It will be expected the increase of safety, reliability and efficiency of maintenance of signaling system by using of the designed communication protocol for railway signaling.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• 제3B권4호
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• pp.195-201
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• 2003

In accordance with the computerization of railway signaling systems, the interface link between signaling equipment has been replaced by a digital communication channel and the importance of this communication link has become increasingly significant. However, there are some problems with the present state of railway signaling. First, different communication protocol is applied to interfaces between signaling although they have the same functions. Next, the communication protocols currently used in the railway fields contain various illogical components such as structure, byte formation, error correction scheme and so on. To solve these matters, a new data link protocol for railway signaling systems is designed. In this paper, the structure of protocol and the results of performance analyses are presented. It will be expected to increase the safety, reliability and efficiency of maintenance of signaling systems by using the designed communication protocol for railway signaling in Korea.

• BMB Reports
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• 제41권10호
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• pp.733-738
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• 2008

Although previous studies have implicated a role for TC1 (C8orf4) in cancer cell proliferation, the molecular mechanism of its action is still largely unclear. In this study, we showed, for the first time, that the mRNA levels of TC1 were upregulated by mitogens (FBS/thrombin) and at least partially, through the ERK1/2 signaling pathway. Interestingly, the over-expression of TC1 promoted the $G_1$- to S-phase transition of the cell cycle, which was delayed by the deficiency of ERK1/2 signaling in fibroblast cells. Furthermore, the luciferase reporter assay indicated that the over-expression of TC1 significantly increased Cyclin D1 promoter-driven luciferase activity. Taken together, our findings revealed that TC1 was involved in the mitogen-activated ERK1/2 signaling pathway and positively regulated $G_1$- to S-phase transition of the cell cycle. Our results may provide a novel mechanism of the role of TC1 in the regulation of cell proliferation.