• Biomedical Science Letters
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• v.7 no.2
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• pp.59-64
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• 2001

Nebulin is an unusually large actin-binding protein specific to the skeletal muscle of vertebrates. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. An SH3 domain occupies the C terminus of nebulin, in the sarcomeric Z-disk and is preceded by a 120-residue stretch containing multiple putative phosphorylation sites. SH3 domain mediates protein-protein interaction involved in the subcellular localization of proteins, cytoskeletal organization and signal transduction. However the binding partner and physiological role of nebulin SH3 domains remains unknown. Using the yeast two-hybrid system, we identified supervillin, an actin-binding protein, as a nebulin SH3 domain-interacting protein. The SH3 domain of nebulin binds to the sequence encoding amino acids 977 to 1335 of supervillin. But the sequence encoding amino acids 977 to 1335 displays weaker binding than the sequence encoding amino acids 977 to 1788.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.1
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• pp.71-74
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• 2005

A LIM protein gene homologue of the CRP (cysteine­rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypep­tide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRPl). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92$\%$ identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.

• Journal of Microbiology and Biotechnology
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• v.12 no.1
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• pp.25-30
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• 2002

A lipase of Staphylococcus aureus B56 was purified from a culture supernatant and its molecular weight was estimated to be 45 kDa by SDS-PAGE. The optimum temperature and pH for the hydrolysis of olive oil was $42^{\circ}C$ and pH 8-8.5, respectively. The enzyme was stable up to $55^{\circ}C$ in the presence of $Ca^++$ at pHs 5-11. The lipase gene was cloned using the PCR amplification method. The sequence analysis showed an open reading frame of 2,076 bp, which encoded a preproenzyme of 691 amino acids. The preproenzyme was composed of a signal sequence (37 aa), propeptide (255 aa), and mature enzyme (399 aa). Based on a sequence comparison, lipase B56 constituted of a separate subgroup among the staphylococcal lipase groups, such as S. aureus PS54 and S. haemolyticus L62 lipases, and was distinct from other lipases in their optimum pH and substrate specificity.

• Journal of Microbiology and Biotechnology
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• v.29 no.11
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• pp.1790-1798
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• 2019

Flock House virus (FHV), an insect RNA virus, has a bipartite genome. FHV RNA1 can be packaged in turnip yellow mosaic virus (TYMV) as long as the FHV RNA has a TYMV sequence at the 3'-end. The encapsidated FHV RNA1 has four additional nucleotides at the 5'-end. We investigated whether the recombinant FHV RNA1 could replicate in mammalian cells. To address this issue, we prepared in vitro transcribed FHV RNAs that mimicked the recombinant FHV RNA1, and introduced them into baby hamster kidney (BHK) cells. The result showed that the recombinant FHV RNA1 was capable of replication. An eGFP gene inserted into the frame with B2 gene of the FHV RNA1 was also successfully expressed. We also observed that eGFP expression at the protein level was strong at 28℃ but weak at 30℃. Sequence analysis showed that the 3'-ends of the RNA1 and RNA3 replication products were identical to those of the authentic FHV RNAs. This indicates that FHV replicase correctly recognized an internally-located replication signal. In contrast, the 5'-ends of recombinant FHV RNA1 frequently had deletions, indicating random initiation of (+)-strand synthesis.

• The Transactions of the Korean Institute of Power Electronics
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• v.10 no.5
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• pp.501-507
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• 2005

This paper proposes a Hybrid Random PWM(HRPWM) technique using a LF2407 DSP board in order to spread the power spectra of an induction motor. The proposed method is composed to the PRBS (Pseudo-Random Binary Sequence) with the Lead-Lag random bit and the random triangular carrier for the logical comparison. Also, a DSP generates the random number, the PRBS and the three-phase reference signal, a MAX038 chip operating as frequency modulator generates the random triangular carrier. For verification of the proposed method, the experiments were conducted with a three-phase adjustable speed a.c drives, and the results of simulations and experiments are presented.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.6
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• pp.990-996
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• 2015

This paper proposes an enhanced scheme adding a sign detector at the front of the correlator in STDR (sequence time domain reflectometry) system. We have executed simulations to show the improvement of detection performance in two fault types and various fault locations. Consequently, it can be shown that the proposed scheme improves the detection performance of the location of far-fault without increasing the computational complexity.

• Proceedings of the KIEE Conference
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• 2006.07a
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• pp.163-164
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• 2006

A current differential relay has been used for transmission line protection. The relay may maloperate in the case of a failure of the secondary circuit of a current transformer (CT) because the differential current is produced. This paper presents an algorithm to detect a failure of a CT using the zero-sequence current. If the magnitude of the zero-sequence current is the same as the magnitude of the current of the other healthy phases, a failure of a CT is detected and then the blocking signal is activated. The proposed algorithm prohibit the maloperation of a differential relay in the case of a CT failure and thus increase the security of the relay.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.655-659
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• 2000

A 11.5-kb DNA fragment containing an endo-inulinase gene was cloned from Xanthomonas oryzae #5. It contained a single open reading frame of 3,999bp, encoding a polypeptide composed of signal peptide of 32 amino acids and mature protein of 1,301 amino acids. From the comparison of amino acids sequences with fructan hydrolases, inulinase, levanase and CFTase, the sequence of the endo-inulinase had highly homology of 72% with CFTase of B. circulans, and six highly conserved regions including the ${\beta}-fructosidase$ motif were found.

• Journal of Communications and Networks
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• v.7 no.3
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• pp.243-247
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• 2005

We propose an efficient block least-mean-square (BLMS) adaptive algorithm, in conjunction with error control coding, for direct-sequence code division multiple access (DS-CDMA) systems. The proposed adaptive receiver incorporates decision feedback detection and channel encoding in order to improve the performance of the standard LMS algorithm in convolutionally coded systems. The BLMS algorithm involves two modes of operation: (i) The training mode where an uncoded training sequence is used for initial filter tap-weights adaptation, and (ii) the decision-directed where the filter weights are adapted, using the BLMS algorithm, after decoding/encoding operation. It is shown that the proposed adaptive receiver structure is able to compensate for the signal-to­noise ratio (SNR) loss incurred due to the switching from uncoded training mode to coded decision-directed mode. Our results show that by using the proposed adaptive receiver (with decision feed­back block adaptation) one can achieve a much better performance than both the coded LMS with no decision feedback employed. The convergence behavior of the proposed BLMS receiver is simulated and compared to the standard LMS with and without channel coding. We also examine the steady-state bit-error rate (BER) performance of the proposed adaptive BLMS and standard LMS, both with convolutional coding, where we show that the former is more superior than the latter especially at large SNRs ($SNR\;\geq\;9\;dB$).

• Fisheries and Aquatic Sciences
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• v.6 no.3
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• pp.116-124
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• 2003

Isolation and cloning of seven-band grouper (Epinephelus septemfasciatus) growth hormone cDNA from pituitary gland revealed an open reading frame of 612 bp coding for a pre-growth hormone of 204 amino acids with a 17 amino acid putative signal peptide. Deduced amino acid sequence showed that there was one possible N-glycosylation site at $Asn^{l84}$ and four cysteine residues $(Cys^{52},\;Cys^{160},\;Cys^{177},\;Cys^{185})$ on t e same positions as in some other species where they were involved in the stabilization of the tertiary structure. The seven-band grouper growth hormone (sbgGH) presented a $99.5\%$ amino acid sequence identity with the growth hormone of Epinephelus coioides and contained the conserved hormone domain region. Comparison of growth hormone sequences from evolutionarily diverse species revealed 25 amino acid residues conserved in jawless fishes to modern mammals. It also revealed an evolutionary trend to retain the same polypeptide sequence even in the distantly related animals while allowing alterations to occur in polypeptides of the closely related species. In order to create a recombinant system to produce high levels of the growth hormone, it was expressed in Escherichia coli (BL21) cells. The gel analysis revealed theoretically expected molecular weights for both mature and pre-sbgGHs.