• 한국지구물리탐사학회:학술대회논문집
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• 2004.06a
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• pp.165-169
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• 2004

Marine magnetic survey were carried out at Sokotra Basin offshore Korea between latitudes $31^{\circ}$ 42'32' N and $32^{\circ}$ 46'29' N, and longitudes $123^{\circ}$ 56'26" E and $125^{\circ}$ 49'16" E in order to estimate the depth of basement complex and as well as to configure it surface and produce the thickness of sedimentary sequence at the study area. Two methods have been used for depth estimation and basement configuration: the power spectrum and the 3-D analytical signal. The estimated depths resulted from the power spectrum method range from 1.4 km to 6.0 km for deep sources (basement troughs), and from 0.3 km to 1.75 km for shallow source (basement peaks). An isopach map was prepared for estimating the thickness of the sedimentary sequence at the study area; it ranges from 1.2 to 4.66 km. The estimated depths resulted from the analytic signal method range from 1.0 to 6 km. A basement configuration map was constructed for the study area in the basin. They show a well agreement with the geology of the study area.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.307-310
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• 2003

A gene encoding streptodornase(sdc) from Streptococcus equisimilis H46A was expressed in S. equisimilis H64H sdc under the control of the streptokinase gene promoter. Secretion of the streptodornase was directed by the signal sequences of streptokinase or streptodornase. The expressed streptodornase activity from S. equisimilis H46A sdc transformant with streptokinase promoter - streptodornase coding sequence fusion vector was 2.3 fold higher than that from wild type. Construct of signal sequence region replaced by streptokinase ones was similarly expressed as a wild type. But constructs of skc or lrp core regions of streptokinase promoter streptodornase fusion were similarly expressed as in sdc mutant. In conclusion, improved expression of streptodornase by use of streptokinase promoter required the full length of promoter.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.375-382
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• 2010

The available techniques for heterologous protein secretion in Lactobacillus strains are limited. The aim of the present study was to develop an efficient protein-secretion system using recombinant lactobacilli for various applications such as live delivery of biotherapeutics. For the construction of expression vectors, the Lactobacillus brevis slpA promoter, Lactobacillus casei prtP signal sequence, and mouse IL-10 sequences were used as a model system. Interestingly, the slpA promoter exhibited strong activity in L. casei, contrary to previous observations. In order to stabilize replication of the plasmid in E. coli, a removable terminator sequence was built into the promoter region. For the improvement of secretion efficiency, a DTNSD oligopeptide was added to the cleavage site of signal peptidase. The resulting plasmids provided remarkably efficient IL-10 secretion. Accumulation of the protein in the culture supernatant varied widely according to the pH conditions. By analysis of the secreted protein, formation of homodimers, and biological activity, IL-10 was confirmed to be functional. The presently constructed plasmids could be useful tools for heterologous protein secretion in L. casei.

• Journal of Broadcast Engineering
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• v.13 no.3
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• pp.289-298
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• 2008

We propose an SNR-scalable coding algorithm for three-dimensional mesh sequences based on singular value decomposition (SVD). SVD achieves a coding gain by representing a mesh sequence with a small number of basis vectors and singular values. First, we introduce a bit plane coding scheme and derive a quantitative relationship between each bit plane and the reconstructed image quality. Using the relationship, we develop a rate-distortion (RD) optimized coding algorithm. Moreover, we propose prediction techniques to exploit the spatio-temporal correlations in real mesh sequences. Simulation results demonstrate that the proposed algorithm provides significantly better RD performance than conventional SVD coders.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.11a
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• pp.55-64
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• 1996

mouse chromesome2에 있는 agouti locus는 정상적으로는 털색깔을 조절하는 gene이다. mouse agouti gene은 최근에 cloning 되었고 131 amino acid peptide와 consensus signal peptide를 encode한다고 보고되었다. 이 논문에서 interspecies-DNA hybridization approach를 이용하여 mouse agouti gene의 human homologue를 cloning 하였다. Sequence analysis 결과, 이는 mouse gene에 85% 유사하였고 consensus signal peptide sequence 를 포함하는 132 amino acid를 coding하였다. somatic-cell hybrid mapping pannel과 Fluorescence-in-situ hybridization에 의한 chromosomal mapping을 한 결과, agouti gene은 MODY (maturity onset diabetes of the young), myeloid leukemia locus 등이 위치한 human chromosome 20q 11.2에 mapping 되었다. 성인 tissue로부터 추출한 RNA를 이용한 발현연구에 의하면 human agouti gene은 adipose tissue와 teatis에 발현되었다.

• Progress in Superconductivity and Cryogenics
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• v.14 no.1
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• pp.44-47
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• 2012

The substantial improvement of the signal-to-noise ratio (SNR) can be achieved with small-size samples or low-field MRI system by high-temperature superconducting(HTS) RF coil. The typical HTS RF coil made of HTS thin film is expensive and is limited the coil geometry to planar surface coil. In this study, commercial Bi-2223 HTS tapes was used as RF coil for a 0.35T permanent MRI system. It has advantages of both much lower cost and easier fabrication over HTS thin film coil. SNR gain of the image obtained from the HTS RF coil over a conventional Cu RF coil at room temperature was about 2.4-fold and 1.9-fold using the spin echo pulse sequence and gradient echo pulse sequence respectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.1
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• pp.115-121
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• 2012

The bumblebee Bombus terrestris is widely used in greenhouses to pollinate crops. Here, we report the molecular cloning and characterization of chymotrypsin inhibitor and chitin-binding protein homologs from B. terrestris. Two cDNAs encoding chymotrypsin inhibitor (Bt-CI) and chitin-binding protein (Bt-CBP) homologs were cloned from B. terrestris. Gene sequence analysis showed that Bt-CI gene consists of three exons encoding 75 amino acids, including a predicted 20-amino acid signal peptide, while Bt-CBP consists of two exons encoding 78 amino acids, including a predicted 26-amino acid signal peptide. The mature Bt-CI and Bt-CBP peptides contain ten and six conserved cysteine residues, respectively. Database searches using the deduced sequences of Bt-CI and Bt-CBP showed similarity to those from B. impatiens (96% peptide sequence identities). Bt-CI and Bt-CBP were expressed in both the venom gland and fat body of B. terrestris worker bees. The recombinant Bt-CI and Bt-CBP peptides were expressed in baculovirus-infected insect cells. Taken together, our findings describe the molecular characterization of Bt-CI and Bt-CBP from B. terrestris.

• Speech Sciences
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• v.10 no.1
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• pp.95-105
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• 2003

This paper describes a new parameter for voice activity detection which serves as a front-end part for automatic speech recognition systems. The new parameter called run-ratio is derived from the runs test statistic which is used in the statistical test for randomness of a given sequence. The run-ratio parameter has the property that the values of the parameter for the random sequence are about 1. To apply the run-ratio parameter into the voice activity detection method, it is assumed that the samples of an inputted audio signal should be converted to binary sequences of positive and negative values. Then, the silence region in the audio signal can be regarded as random sequences so that their values of the run-ratio would be about 1. The run-ratio for the voiced region has far lower values than 1 and for fricative sounds higher values than 1. Therefore, the parameter can discriminate speech signals from the background sounds by using the newly derived run-ratio parameter. The proposed voice activity detector outperformed the conventional energy-based detector in the sense of error mean and variance, small deviation from true speech boundaries, and low chance of missing real utterances

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2000.12a
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• pp.209-212
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• 2000

This paper describes the design and implementation of a hard- wired AGV controller using CPLD(Complex Programmable Logic Device). The proposed controller manages a guidance equipment, motor and I/O sequence controller for a self-control traveling. Compared with a conventional $\mu$-processor, the CPLD controller using a hard-wired control method can reduce a difficult programming process. Also, the total costs of production are reduced, such as development time, product's size and difficulty because memory, combinational logic and sequential logics are implemented by CPLD. The Controller designed using behavioral description method with VHDL and was synthesized by MAX＋Plus II of the ALTERA co. We implemented controller using EPF10K10LC84-4 device.

• Journal of Welding and Joining
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• v.16 no.3
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• pp.129-140
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• 1998

An interface system was developed to offer the welding capability to a robot controller which had not any embedded function for arc welding before, and also an arc sensor algorithm was proposed for weld seam tracking of the welding robot. For the interface system between the robot controller and welding equipments, data communication software and interface connections were composed. The interface system was mae to correspond welding condition, correction data, operation sequence and current status with the robot controller by mutual had shaking and digital signal transfer. Graphic user interface program developed under the environment of windows made it easy to monitor data communication and operation status, and to control welding and sensing sequence. Arc sensing algorithm proposed in this study to compensate torch position error was based on a fuzzy logic with the variables of current difference and current differenced change at torch weaving extremities. The developed interface system could be successfully implemented in between welding equipments and the robot controller, and showed normal status and exact function in data and signal communication between the systems. The whole robot welding system was then examined to verify its welding and seam tracking capabilities in horizontal fillet, vertical fillet, and 3-dimensional fillet weldment. The experiments revealed sound weld bead shapes and also good seam tracing results.