• Korean Journal of Microbiology
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• v.49 no.3
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• pp.215-220
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• 2013

Against environmental stresses, many bacteria utilize the alternate sigma factor RpoS that induces transcription of the specific set of genes helpful in promoting bacterial survival. Intracellular levels of RpoS are determined mainly by its turnover through proteolysis of ClpXP protease. Delivery of RpoS to ClpXP strictly requires the adaptor protein RssB. The two-component-type response regulator RssB constantly interacts with RpoS, but diverse environmental changes inhibit this interaction through modification of RssB activity, which increases RpoS levels in bacteria. This review discusses and summarizes recent findings on regulatory factors in RssB-RpoS interactions, including IraD, IraM, IraP anti-adaptor proteins of RssB and phosphorylation of N-terminal receiver domain of RssB. New information shows that the coordinated regulation of RssB activity in controlling RpoS turnover confers efficient bacterial defense against stresses.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2003.10a
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• pp.168-172
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• 2003

The effects of injection conditions on the droplet sizes resulting from the disintegration of a liquid jet by a fast annular gas stream have been investigated using PDPA. The gas/liquid momentum ratio M = $\rho$$_{g}$ $U_{g}$$^2$/$\rho$$_1$ $U_1$$^2$ and Weber number We = $\rho$$_{g}$ $g^2$ $D_1$/$\sigma$ are selected as key parameters in atomization of shear coaxial spray from the fluid mechanics standpoint. It is revealed that SMD( $D_{32}$) varies inversely with gas/liquid momentum ratio(M), whereas Weber number(We) has little effect on the droplet sizes as gas velocities increase. It is found that gas/liquid momentum ratio is more dominant factor controlling the breakup and atomization process of shear coaxial spray. Finally, an empirical correlation between SMD and injection conditions(i.e. gas/liquid momentum ratio M and Weber number We) is proposed based on the experimental results.

• Journal of Korean Society for Quality Management
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• v.42 no.4
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• pp.665-684
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• 2014

Purpose: The purpose of this study was to test and verify on the interrelationship among Service Quality, and Customer Satisfaction and Customer Loyalty in the Humidifier Goods industry. Especially, we study on the relative importance of each CS variables by distribution channel. Nowadays, most of manufacturing companies are proceeding the product servitization, which is combined service contents with product characteristics in the field of manufacturing industries. Methods: We surveyed humidifier purchaser about the some of CS variables and analyzed it using SPSS 21.0 and AMOS 21.0 as a statistical analysis tool. We use the frequency analysis, confirmatory factor analysis, multi-regression analysis and structural equation modeling analysis for our empirical study. Results: As the result of study, we find that Service Quality(SQ) influence Customer Satisfaction(CS) and CS influence Customer Loyalty(CL). Also, we verify that CS works mediate interaction on the effects between SQ and CL. And there are the significant relative effect of SQ to Customer Satisfaction in some of distribution channel. Conclusion: Finally, we make certain that the market size of the Humidifier goods industry will be increased rapidly. And the distribution channe l is more important to medium and small-sized enterprise. So, we have to pay attention to the study on the distribution channel of the Humidifier goods industry. And then, we expect more researches and studies on Service Quality and Customer Satisfaction in the field of manufacturing industries.

• Transactions of the Korean Society of Mechanical Engineers
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• v.10 no.1
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• pp.25-33
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• 1986

In this paper, the frontal method based elastic-plastic F.E.M. program for mini-computer was developed. Since, the executable source program size was restricted by the system core memory size on the mini-computer, the active variables were memorized by the element base and the nonactive varables were memorized to the external disc file. The active variables of the finally developed program were reduced enough to execute about 1,000 freedom finite element on the mini-computer on which available variables were restricted as 32,767 integers. A modified CT fracture test specimen was examined to test the developed program. The calculated results were compared with experimental results concerning on the crack tip plastic deformation zone. Recrystallization technique was adopted to visualize the intensive plastic deformation regions. The Von-Mises criterion based calculation results were well agreed with the experimental results in the intensive plastic region which was over than 2% offset strain. The F.E.M. results using the developed program were well agreed with the theoritical plastic boundary which was calculated by the stress intensity factor as r$_{p}$=(K$_{1}$$^{2}$/2.pi..sigma.$_{y}$$^{2}$).f(.theta.).).).

• Information Systems Review
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• v.9 no.1
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• pp.311-341
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• 2007

This paper presents a case study on BSC(Balanced Scorecard) system development of Korea Post as a Korean public firm. BSC models of public or private sector are generally developed in different types due to their different strategic goals. Korea Post as a government firm has dual characteristics of BSC similar to both public and private sector. This firm has the organizational type of public firms, not private firms. To date many government subsidiary organizations have already introduced BSC or are trying to introduce BSC, but have difficulty in developing BSC successfully because of insufficient understanding of BSC. Based on a case study of Korea Post, this study analyzed key success and failure factors and suggested prospects on BSC system development of public firms.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.1297-1300
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• 2008

A conventional linear accelerator system requires a flat-topped pulse with less than ${\pm}$ 0.5% ripple to meet the beam energy spread requirements and to improve pulse efficiency of RF systems. A pulse transformer is one of main determinants on the output pulse voltage shape. The pulse transformer was investigated and analyzed with the pulse response characteristics using a simplified equivalent circuit model. The damping factor ${\sigma}$ must be >0.86 to limit the overshoot to less than 0.5% during the flat-top phase. The low leakage inductance and distributed capacitance are often limiting factors to obtain a fast rise time. These parameters are largely controlled by the physical geometry and winding configuration of the transformer. A rise time can be improved by reducing the number of turns, but it produces larger pulse droop and requires a larger core size. By tradeoffs among these parameters, the high-voltage pulse transformer with a pulse width of 10 ${\mu}s$, a rise time of 0.84 ${\mu}s$, and a pulse droop of 2.9% has been designed and fabricated to drive a klystron which has an output voltage of 284 kV, 30-MW peak and 60-kW average RF output power. This paper describes design optimization of a high-voltage pulse transformer for high-power pulsed applications. The experimental results were analyzed and compared with the design. The design and optimal tuning parameter of the system was identified using the model simulation.

• Journal of Acupuncture Research
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• v.20 no.1
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• pp.144-158
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• 2003

This study was carried out to investigate what kinds of statistical techniques have been used to analyze data from oriental medicine research, For study, 551 original articles which used statistical techniques in their data analysis were selected form the articles published in The journal of Korean Acupuncture & Moxibustion Society(JKAMS) between 1984 to 2002. among them, 122 articles used descriptive statistics while 429 articles used inferential statistics for data analysis. For that 429 articles, t-test (189 articles), analysis fo variance (111 articles), chi-square test (14 articles), correlation (10 articles), regression analysis (4 articles), factor analysis(5 articles), or nonparametric test (23 articles) were chose to analyze the data. Nonparametric approach has substantial power in case data do not meet the assumption of normality. This method is not only easy to use ut also provides measures of the statistical variation of nominal and ordinal scale. This study shows that more and more recent papers use nonparametric test compared to the old articles. nine different statistical software or packages (SAS, SPSS, Statview, Minitab, Sigma plot, ISP, Graphpad prism, Excel, Access) have been used in the articles published JKMAS. High level statistical techniques such as SAS, SPSS, and Statview are user friendly and used most for acupuncture and Moxibustion research. Including tables and plots in an article facilitates understanding family process data from a descriptive standpoint, minimized erroneous statistical conclusions, and clarifies theoretically important relationships among variables. Table and plots have been used 500 and 233 articles, respectively. A computer procedure is proposed and illustrated with statistical packages using SAS, SPSS, Statview and ISP.

• International Journal of Oral Biology
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• v.39 no.4
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• pp.207-213
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• 2014

Antimicrobial actions of reactive oxygen/nitrogen species (ROS/RNS) derived from products of NADPH oxidase and inducible nitric oxide (NO) synthase in host phagocytes inactivate various bacterial macromolecules. To cope with these cytotoxic radicals, pathogenic bacteria have evolved to conserve systems necessary for detoxifying ROS/RNS and repairing damages caused by their actions. In response to these stresses, bacteria also induce expression of molecular chaperones to aid in ameliorating protein misfolding. In this study, we explored the function of a newly identified chaperone Spy, that is localized exclusively in the periplasm when bacteria exposed to conditions causing spheroplast formation, in the resistance of Salmonella Typhimurium to ROS/RNS. A spy deletion mutant was constructed in S. Typhimurium by a PCR-mediated method of one-step gene inactivation with ${\lambda}$ Red recombinase, and subjected to ROS/RNS stresses. The spy mutant Salmonella showed a modest decrease in growth rate in NO-producing cultures, and no detectable difference of growth rate in $H_2O_2$ containing cultures, compared with that of wild type Salmonella. Quantitative RT-PCR analysis showed that spy mRNA levels were similar regardless of both stresses, but were increased considerably in Salmonella mutants lacking the flavohemoglobin Hmp, which are incapable of NO detoxification, and lacking an alternative sigma factor RpoS, conferring hypersusceptibility to $H_2O_2$. Results demonstrate that Spy expression can be induced under extreme conditions of both stresses, and suggest that the protein may have supportive roles in maintaining proteostasis in the periplasm where various chaperones may act in concert with Spy, thereby protecting bacteria against toxicities of ROS/RNS.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.28-34
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• 2008

Biofilm formation in association with the intercellular adhesion (icaADBC) gene cluster is a serious problem in nosocomial infections of Staphylococcus aureus. In all 112 S. aureus strains tested, the ica genes were present, and none of these strains formed biofilms. The biofilm formation is known to be changeable by environmental factors. We have found about 30% of phase variation in these strains with treatment of tetracycline, pristinamycin, and natrium chloride. However, this phenotype disappeared without these substances. Therefore, we have constructed stable biofilm-producing variants through a passage culture method. To explain the mechanism of this variation, nucleotide changes of ica genes were tested in strain S. aureus 483 and the biofilm-producing variants. No differences of DNA sequence in ica genes were found between the strains. Additionally, molecular analysis of three regulatory genes, the accessory gene regulator (agr) and the staphylococcal accessory regulator (sarA), and in addition, alternative transcription factor ${\sigma}^B$ (sigB), was performed. The data of Northern blot and complementation showed that SigB plays an important role for this biofilm variation in S. aureus 483 and the biofilm-producing variants. Sequence analysis of the sigB operon indicated three point mutations in the rsbU gene, especially in the stop codon, and two point mutations in the rsbW gene. This study shows that this variation of biofilm formation in S. aureus is deduced by the role of sigB, not agr and sarA.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.12
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• pp.1232-1237
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• 2009

The ICAO and FAA are developing and verifying of GBAS for civil aircraft landing and take-off. The guarantee of aircraft integrity issue is the important part of GBAS. To guarantee integrity, the GBAS ground facility broadcasts various informations to aircraft. The informations are related to the estimated accuracy of each pseudorange correction and the estimated error terms, for example B-value and standard deviation of the ground facility error. These parameters are used to calculate position error (estimated value of the user). If estimated position errors don't satisfy requirements, aircraft use alternate navigation means. In this paper, GBAS reference stations's real data, which operated by KARI (Korea Aerospace Research Institute) in Jeju international airport, are used to development of new ground facility error standard deviation model. We verify improvement of GBAS availability, with respected to vertical protection level, using B-value based a new ground facility error standard deviation model and a sigma inflation factor.