• Journal of Microbiology and Biotechnology
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• 제1권1호
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• pp.37-44
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• 1991

A shuttle promoter-probe vector, pEB203, was derived from pBR322, pPL703 and pUB110. Using the vector, a useful DNA fragment, 319 bp EcoRI fragment, having strong promoter activity has been cloned from Bacillus subtills chromosomal DNA. Selection was based on chloramphenicol resistance which is dependent upon the introduction of DNA fragments allowing expression of a chloramphenicol acetyl transferase gene. The nucleotide sequence of the 319 bp fragment has been determined and the putative -35 and -10 region, ribosome binding site, and ATG initiation codon were observed. This promoter was named EB promoter and the resultant plasmid which can be used as an expression vector was named pEBP313. The crystal protein gene from B. thuringiensis subsp. kurstaki HD-73 was cloned downstream from the EB promoter without its own promoter. When the resultant plasmid, pBT313, was introduced into Escherichia coli and B. subtilis, efficient synthesis of crystal protein was observed in both cells, and the cp gene expression in B. subtilis begins early in the vegetative phase. The cell extracts from both clones were toxic to Hyphantria cunea larvae.

• 한국기계가공학회지
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• 제6권3호
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• pp.47-52
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• 2007

LMTT (Linear Motor based Transfer Technology), which is a new type transfer system used in the maritime container terminal for the port automation, is driven by PM LSM (Permanent Magnetic Linear Synchronous Motor), and largely consists of a controller, shuttle car, and rail. The shuttle car is divided into the frame part, the driving part, and wheels. Because the shuttle car is supported by four wheels in opposition to have five times length for the width of it, a larger deflection than other transfer system using the linear motor occurs. This deflection changes the gap between the mover and the stator, and then brings on an ununiform thrust force. So in this study, we dealt with the structural design for the mover of the shuttle car to generate the uniform thrust force for the efficient control of it. For the investigation, the thickness for each beam of the mover was adopted as design variables, the weight of the mover as objective function, and stress and deflection of the mover as constraint condition.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.850-855
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• 2013

Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.

• Food Science and Biotechnology
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• 제18권2호
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• pp.396-401
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• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.

• 대한안전경영과학회지
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• 제10권4호
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• pp.199-207
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• 2008

Many logistics enterprises have made efforts to achieve low costly and high efficient logistics network. The cross-docking system can be a good solution for them. However, it requires tight schedule and all-night operation inevitably for realization of ideal cross-docking. These causes the difficulty of the attainment of daily delivery target and the leave of delivery service persons. In this paper, we develop the line-haul and shuttle service compromised cross-docking model in order to solve the problems practically. We apply the storage process with the cross-docking system and the direct cross-docking between line-haul and shuttle services. The simulation model validates the shorter delivery time by the developed model than the present model.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.2
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• pp.321-324
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• 2006

LMTT(Linear Motor based Transfer Technology) is a new type of transfer system used in the maritime container terminal for the port automation, and largely consists of a controller, shuttle car, and rail. The shuttle car is divided into the frame part, the driving part, and wheels. In order to design this system, various researches on each part of it must be conducted. In this study, we dealt with the optimum design for the mover of the shuttle car designed from previous studies on the strength of the frame with respect to the number of cross beams to minimize the weight of the shuttle car and to satisfy design criteria of cargo-handling systems in container terminal. For the optimization of the mover, thicknesses of each beam were adopted as design variables, the weight of the frame as objective function, and stress and deflection per unit length as constraint condition.

• 한국정밀공학회지
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• 제18권1호
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• pp.89-97
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• 2001

A microgyroscope, which vibrates in two orthogonal axes on the substrate plane, is designed and fabricated. The shuttle mass of the vibrating gyroscope consists of two parts. The one is outer shuttle mass which vibrates in driving mode guided by four folded springs attached to anchors. And the other is inner shuttle mass which vibrates in driving mode as the outer frame does and also can vibrate in sensing mode guided by four folded springs attached to the outer shuttle mass. Due to the directions of vibrating mode, it is possible to fabricate the gyroscope with simplified process by using polysilicon on insulator structure. Fabrication processes of the microgyroscope are composed of anisotropic silicon etching by RIE, gas-phase etching (GPE) of the buried sacrificial oxide layer, metal electrode formation. An eletromechanical model of the vibrating microgyroscope was modeled and bandwidth characteristics of the gyroscope operates at DC 4V and AC 0.1V in a vacuum chamber of 100mtorr. The detection circuit consists of a discrete sense amplifier and a noise canceling circuit. Using the evaluated electromechanical model, an operating condition for high performance of the gyroscope is obtained.

• 한국추진공학회지
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• 제23권4호
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• pp.50-60
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• 2019

본 논문에서는 MATLAB/Simulink를 기반의 우주왕복선 주엔진(Space Shuttle Main Engine, SSME)의 툴박스 개발을 다루었다. 로켓엔진의 수학적 모델링에는 많은 시간과 신뢰성 확보가 필요하다. 로켓엔진 시뮬레이션 툴박스를 개발하면 이를 해결할 수 있으며 업그레이드와 새로운 엔진 개발의 용이성을 기대할 수 있다. SSME의 수학적 모델링은 7개의 지배방정식을 통해 유도한 32개의 미분방정식을 사용하여 구성하였으며 이를 구성요소 별로 모듈을 구별하여 SSME 시뮬레이션 툴박스를 개발하였다. 성능검증을 위하여 기존 시뮬레이션 결과와 비교하여 검증하였다.

• 한국융합학회논문지
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• 제10권4호
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• pp.195-201
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• 2019

유산소성 운동수행능력의 필드테스트로 대표되는 셔틀런 테스트와 요요테스트를 최대산소섭취량 및 다른 주행테스트들과 비교 검토함으로써 대학 축구선수들의 심폐지구력을 평가하는 유효한 필드테스트를 제안하는 것을 목적으로 하였다. 셔틀런 테스트는 휴식구간이 없이 지속적으로 달려야 하는 반면 요요 테스트의 경우 휴식구간이 설정되어 있어 휴식기를 활용하여 컨디션을 조절하며 주행한다는 특성이 있다. 따라서 요요테스트는 주행과 회복을 반복하는 간헐적 운동을 주로 하는 축구선수들의 종목 특성을 고려한 심폐기능 평가 시 보다 효율적인 현장검사로 활용될 수 있을 것이다.

• 한국기계가공학회지
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• 제18권4호
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• pp.62-68
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• 2019

The aim of this study was to interpret the structure and dynamics of a transfer shuttle system as a material supply device for small machine tools. The following conclusions were obtained by performing a structural interpretation of the material supply equipment with respect to workload and the dynamical interpretation of a flexible multibody carriage shuttle. When a 1,000-kg workload was applied to a fork lift, the safety factor was approximately 1.86. To conservatively assess the integrity of the structure, a 1,000-kg workload would be proper. In the case of a deflection of the fork system, the width increased with increasing time. The greatest deflection occurred at 5.5 s, which was the largest increase in the time point of the fork system.