• Journal of Applied Biological Chemistry
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• v.48 no.4
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• pp.178-182
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• 2005

Nitric oxide (NO) produced from endothelial cells plays a critical role in vascular physiology. The regulation of endothelial NO synthase (eNOS) involves various mechanisms including multiple Ser/Thr phosphorylations. Recently, eNOS-Ser617 was newly recognized to be phosphorylated in response to humoral factors including vascular endothelial growth factor. However, it remains unknown whether and how eNOS-Ser617 phosphorylation is stimulated by shear stress, the primary stimulus of endothelial NO production. This issue was explored in the present study using cultured bovine aortic endothelial cells (BAECs). Over-expression of a constitutively active protein kinase B(Akt) mutant in BAECs increased Ser617 phosphorylation while constitutively active protein kinase A mutant had no effect. When BAECs were subjected to an arterial level of laminar shear stress, eNOS-Ser617 phosphorylation was clearly increased in a time-dependent manner. Shear stress also stimulated Akt phosphorylation at Thr308, one of the key regulatory sites. The time courses of eNOS-Ser617 and Akt-Thr308 phosphorylations appeared to be very similar. These results suggested that eNOS-Ser617 phosphorylation, mediated by Akt, is a physiological response to the mechanical shear stress, involved in the regulation of NO production in endothelial cells.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.13 no.2
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• pp.177-188
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• 2001

The paper presents the method to estimate the bottom shear stress driven by waves and current on rough turbulent flow. Parameter adjusting technique is suggested for the computation of bed shear stress driven by uni-directional flow, and the value ofpararneter is determined by comparing the computational results against Bijker's laboratory data. For the computation of combined flow bottom shear stress, two methods are presented; one is the modified Bijker approach (BYO Model) and the other is the modified Fredsoe approach (FY Model), both of which are refined by the present writers. BYO model is again refined in the computation of maximum shear stress, and the final version is tested against Bijkcr's laboratory data.

• Journal of the Korean Society of Visualization
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• v.20 no.2
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• pp.45-54
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• 2022

We analyzed the low wall-shear stress area in the intracranial aneurysm that occurred at an anterior communicating artery with a special emphasis on vortical structures close to the wall. We reconstructed the aneurysm model from patient CTA data. We assumed blood as an incompressible Newtonian fluid and treated the blood vessel as a solid wall. The pulsatile boundary condition was applied at the inlet of the anterior cerebral artery. From the instantaneous flow field, we computed the histogram of the wall-shear stress over the aneurysm wall and found the low wall-shear stress event (< 0.4 Pa). This extreme event was due to the low wall-shear stress area that occurred at the daughter sac. We found that the merging of two vortices induced the low wall-shear stress area; one arises from the morphological characteristics of the daughter sac, and the other is formed by a jet flow into the aneurysm sac. The latter approaches the daughter sac, which ultimately leads to the strong ejection event near the daughter sac.

• Geomechanics and Engineering
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• v.28 no.2
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• pp.135-143
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• 2022

Soils are natural granular materials whose mechanical properties differ according to the size and composition of the particles, so soils exhibit an obvious scale effect. Traditional soil mechanics is based on continuum mechanics, which can not reflect the impact of particle size on soil mechanics. On that basis, a matrix-reinforcing-particle cell model is established in which the reinforcing particles are larger-diameter sand particles and the matrix comprises smaller-diameter bentonite particles. Since these two types of particles deform differently under shear stress, a new shear-strength theory under direct shear that considers the stress concentration and bypass phenomena of the matrix is established. In order to verify the rationality of this theory, a series of direct shear tests with different reinforcing particle diameter and volume fraction ratio are carried out. Theoretical analysis and experimental results showed that the interaction among particles of differing size and composition is the basic reason for the size effect of soils. Furthermore, the stress concentration and bypass phenomena of the matrix enhance the shear strength of a soil, and the volume ratio of reinforcing particles has an obvious impact on the shear strength. In addition, the newly proposed shear-strength theory agrees well with experimental values.

• Transactions of the Korean Society of Mechanical Engineers
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• v.9 no.4
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• pp.403-413
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• 1985

Hot-wire measurements of second and third-order mean products of velocity fluctuations have been made in the separated, reattached, and redeveloping boundary layer behind a vertical fence. Mean velocity, wall static pressure distributions have also been measured in the whole flow field. Upstream of the reattachment point, the separated shear layer developes as a free mixing layer, but the gradient of the maximum slope thickness, turbulent intensities and the Reynolds shear stress are higher than that of the mixing layer due to initial streamline curvature and the effects of highly turbulent recirculating flow region. In the reattachment region, Reynolds shear stress and triple products near the surface is far more rapid than the decrease of the shear stress; that is the presence of the solid wall has a marked effect on the apparent gradient diffusivity of intensity or shear stress and throws doubts upon the usefulness of the simple gradient diffusivity model in this region.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.12
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• pp.3369-3376
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• 1994

A finite element program for elastic stress wave propagation is developed in order to investigate the shape of stress field and analysis the magnitude of stress wave intensity at time increment. Accuracy and reliance of the finite element analysis are acquired when the element size is smaller than the product of the stress wave speed and the critical value of increasing time step. In the finite element analysis and theoretical solution, the longitudinal stress wave is propagated to the similar direction of impact load, and the stress wave intensity is expressed in terms of the ratio of propagated area. The direction of shear wave is declined at an angle of 45 degrees compared with longitudinal stress wave and the speed of shear wave is half of the longitudinal stress wave.

• Journal of the Korean Geotechnical Society
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• v.33 no.1
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• pp.79-91
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• 2017

An experimental study has been conducted by using the Cyclic Direct Simple Shear apparatus to evaluate the influence of average and cyclic shear stresses on the undrained shear failure behavior of marine silty sand considering various relative densities. The obtained results show that despite using different relative densities, similar trends were gained in the cyclic shear deformation. Moreover, the cyclic shear deformation is affected mainly by the average and cyclic shear stresses. The number of cyclic loads for failure is significantly affected by the cyclic shear stress ratio and relative density, and is less affected by the average shear stress ratio. The proposed three-dimensional stress-dependent failure contour can be used effectively to assess the soil shear strength considering various relative densities in the design of foundation used for offshore structures.

• Journal of the Korean Geotechnical Society
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• v.22 no.10
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• pp.151-163
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• 2006

This paper presents the interrelationship between undrained static and cyclic shear behavior. Laboratory works were performed through the undralned static and cyclic triaxial test using Nak-Dong River sand. And static triaxial test involved the triaxial extension test for comparison with cyclic shear behavior Cyclic triaxial test was performed with a variety of combination conditions of initial static shear stress $(q_{st})$ and cyclic stress $(q_{cy})$. In this result, the stress path of cyclic shear behavior was correspondent with static shear behavior passing the critical stress ratio (CSR) line because of the development of flow deformation. After that, a failure occurred according to failure line (FL) of static shear behavior. The stress path of cyclic shear behavior showed essentially the same with static shear behavior, although it appears a little different in test method.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.361-364
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• 2004

This paper predicts the shear stress-strain relationship of reinforced concrete columns using Transformation Angle Truss Model (TATM) considered bending moment and axial force effects. Nine columns with various shear span-to-depth ratios and axial force ratios were tested to verify the theoretical results obtained from TATM. Shear stress-strain relationship obtained from TATM was agreed well with test results conducted by bis study than other truss models.

• Structural Engineering and Mechanics
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• v.65 no.3
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• pp.303-314
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• 2018

Shear walls are a typical member under a complex stress state and have complicated mechanical properties and failure modes. The separated-elements model Genetic Evolutionary Structural Optimization (GESO), which is a combination of an elastic-plastic stress method and an optimization method, has been introduced in the literature for designing such members. Although the separated-elements model GESO method is well recognized due to its stability, feasibility, and economy, its adequacy has not been experimentally verified. This paper seeks to validate the adequacy of the separated-elements model GESO method against experimental data and demonstrate its feasibility and advantages over the traditional elastic stress method. Two types of reinforced concrete shear wall specimens, which had the location of an opening in the middle bottom and the center region, respectively, were utilized for this study. For each type, two specimens were designed using the separated-elements model GESO method and elastic stress method, respectively. All specimens were subjected to a constant vertical load and an incremental lateral load until failure. Test results indicated that the ultimate bearing capacity, failure modes, and main crack types of the shear walls designed using the two methods were similar, but the ductility indexes including the stiffness degradation, deformability, reinforcement yielding, and crack development of the specimens designed using the separated-elements model GESO method were superior to those using the elastic stress method. Additionally, the shear walls designed using the separated-elements model GESO method, had a reinforcement layout which could closely resist the actual critical stress, and thus a reduced amount of steel bars were required for such shear walls.