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Studies on the glucose isomerizing enzyme. (Part IV) -On the enzymatic properties and treating conditions for high activity containing cells- (포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) (제4보(第四報)) -효소학적(酵素學的) 성질(性質)및 효소생성능(酵素生成能)에 대(對)하여-)

  • Rhee, In-Koo;Seu, Jung-Hwn
    • Applied Biological Chemistry
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    • v.12
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    • pp.69-74
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    • 1969
  • This glucose isomerizing enzyme, which Actinomyces sp. (strain, K-17) produced, was inhibited by citrate, oxalate, ethylene diamine tetraacetic acid and cysteine on the enzyme reaction. This enzyme isomerized xylose to ketose as well as glucose. The Michaelis constant of this enzyme was $7.2X\;10^{-1}M.$. on D-glucose. The enzyme activity of intact cells which were harvested in the none xylose containing medium was very weak. If these intact cells of low activity were treated in the buffered xylose solution, its activity was considerably increased. After fifteen hours at $32^{\circ}C$. on xylose treatment, the enzyme activity was increased to equilibrium and the treating condition was proper at neutral pH and in aerobic state. The adequate concentration of xylose on the treatment was about 0.5 percent.

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Studies on the application of bacterial sericinase -(Part II) Appling on the unwinding for mulberry cocoon- (세균성(細菌性) Sericin 분해효소(分解酵素)의 이용(利用)에 관(關)한 연구(硏究) -(제II보(第II報)) Sericinase에 의(依)한 가잠견(家蠶繭)의 해서(解舒)에 관(對)하여-)

  • Seu, Jung-Hwn;Song, Bang-Ho;Han, Yung-Gu;Kho, Yung-Hee
    • Applied Biological Chemistry
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    • v.13 no.1
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    • pp.87-92
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    • 1970
  • Sericin hydrolyzing enzyme, produced by the selected bacteria, S4-1-1, was studied and following properties were obtained. 1. The activity of this bacterial sericinase was not decreased for 30 days of storage at $5^{\circ}C$. But at $20^{\circ}C$, for 30 hrs. was the maximum period to keep the initial activity of this enzyme. 2. This bacterial enzyme gave only sericinase activity but never indicated fibroin hydrolyzing activity. 3. The chelating reagent of EDTA and Ag or Hg ions were classified as strung inhibitors but Cu and Cd ions were indicated as moderate inhibitors to this enzyme action. 4. This enzyme was not inhibited by the surface active agent, Peretex-N, but strongly activated by this agent at low concentration. In the other hand, by the application of this enzyme to the unwinding works on the mulberry cocoon, the following results were also obtained. 1. On the weight and length, nonbreaking Length, size, colour and unwinding ratio of have, the enzyme appling method was superior to generally used cooking method. 2. The tested results of strength and elongation, bouchon, haririness loops, neatness and evenness of have were also indicated spuerior properties.

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The Enhancing Effect of Atopic Dermatitis by cosmetics containing estern medical herbs (한방소재 화장품의 아토피 피부염 개선 효과)

  • Kang, Shin-Jyung;Kim, Ae-Jung;Lee, Yeon-Hee;Lee, Myoung-Sook;Joung, Kyung-Hee;Cho, Nam-Ji
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.11
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    • pp.3500-3505
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    • 2009
  • Sixteen moderate atopic dermatitis patients were treated with applying toner and cream using estern medical herbs[Estern medical complex(Radix Glycyrrhizae, Radix Angelicae Dahuricae, Rhizoma Ligustici Chuanxiong, Fructus ponciri Seu Aurantii Immaturus, Radix Rehmanniae Praeparata, Radix Puerariae, Rhizoma Corydalis), Cypress, Green tea, Radix Astragali, Red ginseng, Syrup ferment, Apple ferment filtrate] on the atopic area 2-3 times per day for 6 weeks. After 6 weeks of study, the result was a decrease in SCORAD index($26.9{\pm}11.8$ to $14.9{\pm}9.7$(p<0.05)), IgE($641.9{\pm}1294.6$ to $565.8{\pm}1076.8$), Eosinophil count($246.7{\pm}203.5$ to $203.3{\pm}130.7$(p<0.05)) and TEWL($16.7{\pm}5.1$ to $15.4{\pm}8.7$(p<0.05)). And skin hydration was increased($36.6{\pm}10.0$ to $44.0{\pm}10.3$(p<0.01)) maintaining skin pH level. The patient;s and physician's global assessment also improved. Therefore, estern medical herbs may play a role in treatment of atopic dermatitis.

Studies on the Utilization of Persimmons -(Part 1) Effect of Carton Dioxide Treatment on the Removal of Astringency- (감의 이용(利用)에 관(關)한 연구(硏究) -제1보(第一報) 탄산(炭酸)가스처리(處理)가 탈삽(脫澁)에 미치는 영향(影響)-)

  • Seu, Ohn-Soo;Sohn, Tae-Hwa
    • Applied Biological Chemistry
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    • v.19 no.2
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    • pp.93-98
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    • 1976
  • The experiment was made to investigate concentrations of $CO_2\;and\;O_2$ in the removal of astringency of persimmon fruits and to study the changes of total, reducing sugar and hardness during the removal of astringency. 1. During the removal of astringency of persimmon fruits, channel of tannin content showed the rapid decrease in high concentration of $CO_2$ and the gradual decrease in low concentration of $CO_2$. 2. Concentration of oxygen did not show effect on the removal of astringency at high concentration of $CO_2$ but at low concentration of $CO_2$ in Chungdo-Bansi. 3. Optimal concentration of the removal of astringency was $60{\sim}70%,\;CO_2$ and $6{\sim}8%\;O_2$ in Chungdo-Bansi and Sagoksi 4. In changes of sugar content total sugar was decreased and reducing sugar was increased.

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Anti-wrinkle Effect of Cosmetics Containing Duchesnea indica Extract (사매추출물을 함유하는 화장품의 주름 개선 효과)

  • Yang, Woong-Suk;Kim, Young-Min;Kim, Ee-Hwa;Seu, Young-Bae;Yang, Yoon-Jung;Kim, Hyun-Woo;Kang, Se Chan
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.4
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    • pp.281-288
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    • 2010
  • In this study, we investigated anti-oxidative effects of Duchesnea indica extracts by using Oxygen Radical Absorbance Capacity (ORAC). The extracts were prepared with 0 %, 30 %, 50 %, 70 % and 100 % aqueous ethanol respectively. The 30 % EtOH D. indica extract showed higher ORAC activity than the other extracts. Therefore, we performed in vitro studies on cytotoxicity of NIH-3T3 cells and MMP-8 collagenase inhibition using by the 30 % EtOH extract. The 30 % EtOH extract showed no cytotoxicity and significant inhibition on MMP-8 collagenase. And we performed clinical studies for the anti-wrinkle effect of the Di-Wrinkle Free Cream. The cream formula was prepared with 2 % arbutin and 1 % D. indica extract. Twenty one healthy women volunteers, ages of 35 and 50, applied the cream on their faces twice a day for 8 weeks. The skin was evaluated with PRIMOS (phaseshift rapid in vivo measuring of human skin) system and analyzed by the student's paired t-test. The wrinkles on the eye region were reduced by 13 % based on the PRIMOS system after 8 weeks. In the safety study of the Di-Wrinkle Free Cream, no symptoms were observed such as erythema, edema, scaling, itching, stinging, burning, tightness and prickling by visual observation and medical examination of volunteers for 8 weeks. Moreover, there was no noticeable skin disorder during experience period. These results suggested that D. indica extracts could be applied as cosmeceuticals effective for anti-wrinkle.

Inhibitory Substance Produced by Aspergillus sp. on the Snake Venom Proteinase - Isolation of Microorganism and Biological Activities of the Inhibitor - (Aspergillus 속 균주가 생성되는 사독 Proteinase에 대한 저해물질 - 균의 분리 및 저해물질의 생물학적 작용상 -)

  • Hyun, Nam-Joo;Seu, Jung-Hwn
    • Microbiology and Biotechnology Letters
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    • v.15 no.2
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    • pp.129-134
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    • 1987
  • Aspergillus sp. (MK-24) producing a biological active substance that inhibited the venom proteinase activity was isolated from soil. The substance also inhibited the activity of trypsin and coagulation of blood, but did not inhibit papain, $\alpha$-chymotrypsin and pepsin. The substance was partially purified from culture filtrate by precipitaion with acetone, and by chromatography of DEAE-Sepadex A-50 column and Amberlite IRC-50 ion exchange. The inhibitory substance was stable in the wide pH range from 2.0 to 12.0 at 37$^{\circ}C$, but not stable at $65^{\circ}C$ in the alkaline pH. Only 12% of the activity was decreased by the heat treatment at 10$0^{\circ}C$ for two hours. The inhibition on venom proteinase (Agkistrodon bromohoffi brevicaudus) was a mixed type. The inhibitory activity depended on the preincubation time and completely depressed by cupric, zinc and cobalt ions. The inhibition on the venom proteinase was appeared strongly on casein but not on ovalbumin or hemoglobin as a substrate.

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Some Properties of Polyphenol Oxidase from Apple (Golden Delicious) (사과(골덴) Polyphenol Oxidase의 효소학적(酵素學的) 성질(性質))

  • Chung, Ki-Taek;Seu, Seung-Kyo;Han, Sung-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.2
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    • pp.158-164
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    • 1986
  • Polyphenol oxidase in apple (Golden Delicious) was extracted, partially purified and its properties were found as follows; Polyphenol oxidase showed optimum pH for activity at 6.5 and optimum temperature at $30^{\circ}C$ and high affinity to o-diphenol compounds. Cysteine, ascorbic acid and sodium metabisulfite appeared to be most effective inhibitors. EDTA showed a slight inhibition. During the enzyme was kept in test tube at $4^{\circ}C\;and\;20^{\circ}C$ for a week, polyphenol oxidase activity decreased sharply during the first four days at $20^{\circ}C$, then decreased slowly as the storage was prolonged. At $4^{\circ}C$, the polyphenol oxidase activity appeared to be relatively stable during the first two days before activity began to decline sharply. Polyacrylamide disc gel electrophoresis indicated four bands with polyphenol oxidase activity. Three bands and one band of the active bands were observed after heating for 1hr at $60^{\circ}C\;and\;70^{\circ}C$ respectively. The enzyme activity was observed 40% after treatment at $60^{\circ}C$ and 5% after treatment at $70^{\circ}C$. Therefore, no difference in the thermal stability was observed between active bands and the enzyme activity.

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HIV-1 Tat-mediated protein transduction of human brain creatine kinase into PC12 cells

  • Jeong, Min-Seop;Kim, Dae-Won;Lee, Min-Jung;Lee, Yeom-Pyo;Kim, So-Young;Lee, Sun-Hwa;Jang, Sang-Ho;Lee, Kil-Soo;Park, Jin-Seu;Kang, Tae-Cheon;Cho, Sung-Woo;Kwon, Oh-Shin;Eum, Won-Sik;Choi, Soo-Young
    • BMB Reports
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    • v.41 no.7
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    • pp.537-541
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    • 2008
  • Epilepsy is characterized by the presence of spontaneous episodes of abnormal neuronal discharges and its pathogenic mechanisms remain poorly understood. Recently, we found that the expression of creatine kinase (CK) was markedly decreased in an epilepsy animal model using proteomic analysis. A human CK gene was fused with a HIV-1 Tat peptide to generate an in-frame Tat-CK fusion protein. The purified Tat-CK fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced Tat-CK fusion protein was stable for 48 h. Moreover, the Tat-CK fusion protein markedly increased endogenous CK activity levels within the cells. These results suggest that Tat-CK provides a strategy for the therapeutic delivery of proteins in various human diseases including the delivery of CK for potential epilepsy treatment.

Development of the Dissolution Test for Viquidil Hydrochloride Capsules and Alibendol Tablets (염산비퀴딜 캡슐 및 알리벤돌 정의 용출시험에 관한 연구)

  • Hwang, Joung-Boon;Koo, Eun-Joo;Go, Seu-Youn;Cho, Kyung-Chul;Moon, Hyun-Ju;Cho, Soo-Yeul;Kang, Chan-Soon;Shon, Yeo-Won;Kim, Young-Ok;Sohn, Kyung-Hee;Cho, Dae-Hyun
    • YAKHAK HOEJI
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    • v.54 no.5
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    • pp.348-353
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    • 2010
  • The dissolution test method and an analytical procedure by HPLC were developed and validated for viquidil hydrochloride capsules and alibendol tablets. These drugs were not yet characterized by the dissolution specifications in Korean Pharmaceutical Codex. So, with each reference and test drugs, we did the preliminary and standard experiments based on the Korean Pharmacopeia Guideline of dissolution testing for solid oral dosage forms. The dissolution test for viquidil hydrochloride capsules was carried out under sink conditions as follows: dissolution medium water, paddle rotation speed 50 rpm and vessel volume 900 ml. More than 90% of its label amount was released within 30 min in this method. Also the dissolution test for alibendol tablets was carried out under sink conditions as follows: dissolution medium water, paddle rotation speed 100 rpm and vessel volume 900 ml. More than 90% of its label amount was released within 45 min in this method. The dissolution samples were analyzed with a precise and accurate HPLC method. The developed dissolution test showed specificity, linearity, precision and accuracy within the acceptable range. The dissolution testing method described above was adequate for the purpose and may be proposed as a pharmacopeial standard to assess the performance of viquidil hydrochloride capsules and alibendol tablets.

Studies on the Glucose Isomerizing Enzyme -Part II. Some Properties of the Glucose Isomerizing Enzyme of K-17 strain- (포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) -제2보(第二報); 분리선정(分離選定)된 균주효소(菌株酵素)의 성질(性質)에 대(對)해서-)

  • Seu, J.H.;Kim, C.K.;Ki, W.K.;Rhee, I.K.;Kwon, T.J.;Woo, D.L.
    • Applied Biological Chemistry
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    • v.11
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    • pp.49-53
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    • 1969
  • Some properties of glucose isomerizing enzyme which produced by the strain K-17 in xylose containing nutrient broth medium were investigated. The optimal pH for enzyme reaction was indicated about 7.2 and optimal temperature was about $75^{\circ}C$. The same optimal temprature was indicated by both cell free extract and acetone dried cells using as enzyme. The glucose isomerizing enzyme from strain K-17 was not inhibited by the high concentration of substrate even in a suturated glucose solution, but most enzyme was inactivated by the heat treatment at $80^{\circ}C$. The maximum fructose forming ratio from glucose was about 50 percents.

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