Applied Biological Chemistry

  • Volume 11
  • /
  • Pages.49-53
  • /
  • 1969
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the Glucose Isomerizing Enzyme -Part II. Some Properties of the Glucose Isomerizing Enzyme of K-17 strain-

포도당(葡萄糖) 이성화(異性化) 효소(酵素)에 관(關)한 연구(硏究) -제2보(第二報); 분리선정(分離選定)된 균주효소(菌株酵素)의 성질(性質)에 대(對)해서-

  • Seu, J.H. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kim, C.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Ki, W.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Rhee, I.K. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Kwon, T.J. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University) ;
  • Woo, D.L. (Dept. of Applied Microbiology, Agr. College, Kyung-Pook National University)
  • Published : 1969.04.30
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Abstract

Some properties of glucose isomerizing enzyme which produced by the strain K-17 in xylose containing nutrient broth medium were investigated. The optimal pH for enzyme reaction was indicated about 7.2 and optimal temperature was about $75^{\circ}C$. The same optimal temprature was indicated by both cell free extract and acetone dried cells using as enzyme. The glucose isomerizing enzyme from strain K-17 was not inhibited by the high concentration of substrate even in a suturated glucose solution, but most enzyme was inactivated by the heat treatment at $80^{\circ}C$. The maximum fructose forming ratio from glucose was about 50 percents.

본(本) 연구(硏究)에서 얻어진 결과(結果)를 요약(要約)하면 대략(大略) 다음과 같다. (1) 본균(本菌)의 최적작용(最適作用) pH는 7.2부근(附近)에 위치(位置)하며 (2) 최적작용온도(最適作用溫度)에 있어서는 추출효소(抽出酵素)나 건조균체(乾燥菌體)를 그대로 사용(使用)하거나 다같이 $75^{\circ}C$부근(附近)에 위치(位置) 하며. (3) 본(本) 효소(酪素)는 그 작용(作用)에 있어서 기질(基質)인 Glucose의 농도(濃度)가 포화상태(飽和狀態)에서도 조해(阻害) 현상(現象)을 나타내지 않으며 (4) 본(本) 효소(酵素)의 Glucose에 대(對)한 최고(最高) 이성화율(異性化率)은 약(約) 50%에 달(達)하며 반응조건(反應條件)을 개선(改善)하므로서 더 높은 이성화율(異性化率)을 얻을수 있으리라고 생각되며 (5) 본(本) 효소(酵素)는 $80^{\circ}C$의 온도(溫度)에서는 거의 대부분(大部分)이 실활(失活)됨을 알 수 있었다.

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