Studies on the application of bacterial sericinase -(Part II) Appling on the unwinding for mulberry cocoon-

세균성(細菌性) Sericin 분해효소(分解酵素)의 이용(利用)에 관(關)한 연구(硏究) -(제II보(第II報)) Sericinase에 의(依)한 가잠견(家蠶繭)의 해서(解舒)에 관(對)하여-

Sericin hydrolyzing enzyme, produced by the selected bacteria, S4-1-1, was studied and following properties were obtained. 1. The activity of this bacterial sericinase was not decreased for 30 days of storage at $5^{\circ}C$. But at $20^{\circ}C$, for 30 hrs. was the maximum period to keep the initial activity of this enzyme. 2. This bacterial enzyme gave only sericinase activity but never indicated fibroin hydrolyzing activity. 3. The chelating reagent of EDTA and Ag or Hg ions were classified as strung inhibitors but Cu and Cd ions were indicated as moderate inhibitors to this enzyme action. 4. This enzyme was not inhibited by the surface active agent, Peretex-N, but strongly activated by this agent at low concentration. In the other hand, by the application of this enzyme to the unwinding works on the mulberry cocoon, the following results were also obtained. 1. On the weight and length, nonbreaking Length, size, colour and unwinding ratio of have, the enzyme appling method was superior to generally used cooking method. 2. The tested results of strength and elongation, bouchon, haririness loops, neatness and evenness of have were also indicated spuerior properties.

본(本) Sericin 분해(分解) 효소(酵素) 강력(强力) 분필균주(分泌菌株) $S_4-1-1$이 생성(生成)하는 Sericinase 는 1. 열(熱)에 대(對)해 안정(安定)하여 $5^{\circ}C$에서 1개월간(個月間) $20^{\circ}C$에서 약(約) 30시간(時間)까지 그 활성(活性)이 지속(持續)되며 2. Fibroin 물질(物質)에 대(對)한 분해력(分解力)은 전연(全然) 인정(認定)되지 않았고 3. EDTA 및 $Ag^{++}$, $Hg^{++}$에 의(依)해 거의 완전실활(完全失活)되며 $Cu^{++}$, $Cd^{++}$에 의(依)해서도 비교적(比較的) 강(强)하게 실활(失活)되였다. 4. 계면활성제(界面活性劑) Peretex N의 혼존시(混存時) 그 활성(活性)이 다소(多少) 촉진(促進)되었다. 이와 같은 Sericin분해효소(分解酵素)를 가잠견(家蠶繭)의 해서(解舒)에 이용(利用)하므로서 현행(現行)되고 있는 자견(煮繭)에 비(比)해 1. 조사성적(繰絲成績) 즉 해서율(解舒率), 생사량비율등(生絲量比率等)이 오히려 우수(優秀)했으며 2, 사조반(絲條班), 소절(小節), 인장강도(引長强度)의 성적(成績) 역시(亦是) 자견(煮繭)에 비(比)해 하등(下等)의 손색(遜色)이 없었다.