• Nutrition Research and Practice
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• 제11권4호
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• pp.327-333
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• 2017

BACKGROUND/OBJECTIVES: Complications of diabetes, such as cardiovascular disease, are associated with increased mortality among type 2 diabetes mellitus patients. Homocysteine has been recently identified as a predictor of cardiovascular disease-related complications in diabetes. We investigated whether or not supplementation with folic acid tablets can lower homocysteine levels and improve parameters related with vascular complications. SUBJECTS/METHODS: We conducted a non-randomized 8-week trial involving postmenopausal diabetic women (n = 25) supplemented with $800{\mu}g$ of folic acid ($400{\mu}g$ twice a day) daily. Subjects' serum levels of folate, homocysteine, and vitamin $B_{12}$ were measured, along with vascular function and brachial-ankle pulse wave velocity. RESULTS: Folic acid supplementation significantly increased serum folate levels (P < 0.0001), reduced homocysteine levels (P < 0.0001), and increased vitamin $B_{12}$ levels (P = 0.0063). There were significant decreases in low-density lipoprotein cholesterol levels as well as the ratios of low-density lipoprotein cholesterol to high-density lipoprotein cholesterol and total cholesterol to high-density lipoprotein cholesterol. Brachial-ankle pulse wave velocities were not altered by supplementation. Changes in serum vitamin $B_{12}$ after folic acid supplementation were negatively correlated with changes in brachial-ankle pulse wave velocity. CONCLUSIONS: In this study on postmenopausal Korean women with type 2 diabetes mellitus, folic acid supplementation reduced serum homocysteine levels, increased serum folate and vitamin $B_{12}$ levels, and lowered lipid parameters.

• 대한지역사회영양학회지
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• 제5권2호
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• pp.201-207
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• 2000

This study was performed to assess the nutritional status of nursing home residents and to assess the effect of nutrition intervention. The subjects were 123 people aged over 60 years from 5 different nursing homes. The nutrition intervention study was carried out by supplementing their diet with multivitamin-minerals for 2 months. The mean intakes of most nutrients did not meet the RDA, Though the nutrient content of the menus provided by the facilities were satisfactory. Nutrients of which intakes fell below 75% of the RDA were protein, Ca, Fe, vitamin A vitamin B$_1$, and vitamin B$_2$. The BMIs of male and female subjects were 22.0kg/$m^2$ and 24.6kg/$m^2$ and the WHRs were 0.92 and 0.90, respectively. The percentage of subjects with hypertention (BP$\geq$140/90mmHg) and with anemia(Hb$\leq$13mg/100$m\ell$ in men, Hb$\leq$12mg/100$m\ell$ in women) were 34.6% and 41.9%, respectively. The serum cncentrations of albumin, total protein, triglyceride, total cholesterol, HDL-cholesterol, and total lipid fell within normal ranges. However, 27.5% of the subjects showed a high serum cholesterol level of over 250mg/100$m\ell$. The concentration of C3 was 81.2mg/100$m\ell$, IgG, 1343mg/100$m\ell$, and IL-2, 0.766mg/$m\ell$. after 2 months of vitamin-mineral supplementation, the levels of blood glucose and total cholesterol were significantly decreased and triglyceride was significantly increased. The vitamin-mineral supplementation had no effect on the mean levels of vitamin A and E, IgG, IL2, and C3. However, the intervention resulted int he improvement of serum vitamin A and E levels when the subject\`s serum levels were low before the supplementation.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.525-534
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• 2023

Vitamin C plays an important role for fish survival, growth and disease resistance. However, the optimal vitamin C for rearing red drum Sciaenops ocellatus juveniles in Vietnam is not known. To address this issue, a 70-day feeding trial was conducted to evaluate the optimal dietary vitamin C requirements for red drum juveniles. Seven isonitrogenous (55.35% protein) and isolipidic (9.07% lipid) diets were formulated to include graded vitamin C concentrations of 23.2, 124.5, 235.2, 423.8, 626.7, 824.6, and 1,027.3 mg/kg, respectively. The results showed that fish fed on 423.8 mg/kg vitamin C diet had the highest growth rate, which can be linked to the increased feed utilization. Broken-line analysis indicated that the optimal dietary vitamin C requirements of red drum juveniles were 342.92 and 405.80 mg/kg for growth parameters, feed utilization, body composition and biochemical parameters of serum. Based on these parameters the optimal vitamin C supplementation level for red drum juveniles was estimated in the range of 342.92-405.80 mg/kg vitamin C in the diets with direct applications in producing artificial feed for rearing juveniles of this species in Vietnam.

• Biomolecules & Therapeutics
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• 제5권1호
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• pp.59-66
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• 1997

This study was done to determine that vitamins I and C are synergistic in protecting liver cells during hepatic ischemia and repefusion. Rats treated with vitamins I and C were subjected to 60 min of hepatic ischemia and to 1 and 5 hr of reperfusion thereafter. Serum aminotransferase level and microsomal lipid peroxidation were markedly increased by ischemia/reperfusion. These increases were significantly attenuated by vitamins E, C or its combination. Hepatic wet weight-to-dry weight ratio was increased in ischemic group, but this increase was prevented by combination of vitamin I and C. Bile flow and cholate output were markedly decreased by ischemia/reperfusion and vitamin C alone and combination of vitamin I and C restored their secretion. Cytochrome P-450 content and aminopyrine N-demethylase activity were decreased by ischemia/ reperfusion and restored by vitamin C and combination of vitamin I and C to the level of sham-operated rat. Aniline p-hydroxylase activity was increased by ischemia/reperfusion and this increase was prevented by vitamin E. Our findings suggest that ischemia/reperfusion diminishes hepatic secretory and microsomal functions by increasing lipid peroxidation and vitamins I and C synergistically ameliorates these changes.

• 생명과학회지
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• 제10권3호
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• pp.254-261
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• 2000

난소를 절제한 female rat에 Vit C. Vit E, Vit C와 Vit E를 복합적으로 투여했을 때 rat의 간 조직 의 손상 정도 및 항산화 효소 활성을 관찰하기 위해 체중 150$\pm$10g이 되는 female rat에 100mg/kg 함 량의 각 실험 물질들을 이틀에 한 번 두달 간 복강내로 투여한 뒤 간 조직 중 과산화지질량 및 항상화 효소인 SOD, catalase, 총 glutathion 량 및 Glutathion peroxidase의 활성도를 측정함으로써 항산화계의 변화 및 생리 활성 변화를 살펴 보았다. 1. 간, 신장 조직 중의 과산화지질 함량은 노화군(Ovx)이 정상 군(Sham)에 비해 각각 1.78배, 1.61배 증가하였다. 그리고 각 비타민 투여군은 난소를 절제한 군에 비해 과산화질함량이 현저히 감소하였다. 2. 혈청 중 총 cholesterol 량은 노화군(Ovx)이 정상군(Sham)에 비 해 2.57배 높게 나타났다. 각 비타민 투여군은 노화군에 비해 수치가 현저히 감소하였는데, Ovx+Vit C 군의 경우는 94%정도의 억제율을 보였다. HDL-cholesterol은 노화군(Ovx)이 정상군(Sham)에 비해 36.7% 감소하였다. 각 비타민 투여로 HDL-cholesterol 함량이 좋은 수준으로 증가되었고, Ovx+Vit Erns은 노화군보다 2.52배 높게 나타났다. 동맥경화지수도 Ovx+Vit E 군이 아주 낮게 나타났다. 3. 간 조직 중 SOD 활성도는 노화군(Ovx)이 정상군(Sham)보다 5.3배 높게 나타났고, 각 비타민 투여로 free radical 생성이 억제되어 항산화효소인 SOD 활성도를 감소시켰다. 간 조직 중 catalase 활성도는 노화군 (Ovx)이 정상군(Sham)보다 1.58배 정도 높게 나타났는데, 각 비타민 투여로 catalase 활성도가 낮아졌 고, Ovx+Vit E 군은 탁월한 감소 효과를 보였다. 4. 간 조직 중 총 glutathion의 활성도는 정상군 (Sham)이 노화군(Ovx)에 비해 1.22배 정도 높게 나타났고, 각 비타민 투여군 중 Ovx+Vit Erns의 경우 간 조직 중 총 glutathionfid이 가장 높게 나타났다. 간조직 중 reduced-glutathion (GSH)함량과 총 glutathion 량에 대한 oxidized-glutathion (GSSG)의 비율, 또한 Ovx+Vit E 군의 수치가 가장 높게 나 타났다. Vit E가 항산화에 좋은 효과가 있음을 알 수 있었다. 그러나, GPx의 활성 수치는 그 다지 유의 한 변화를 나타내지는 않았다.

• 한국체육학회지인문사회과학편
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• 제54권5호
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• pp.727-737
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• 2015

본 연구는 사무직 근로자를 대상으로 비타민 D 상태와 생활습관위험인자간의 연관성을 조사하고자 하였다. S시의 30세 이상 사무직 근로자 515명(남 336명, 여 179명)을 대상으로 혈청 비타민 D 수준, 비만지표, 체력, 대사성위험인자 및 혈중 지질 그리고 좌식습관을 포함한 생활습관위험인자를 측정하였다. 혈청 비타민 D 수준을 기준으로 결핍(<20 ng/mL), 부족(20-29 ng/mL), 충분(≥30 ng/mL) 집단으로 구분하고, 집단 간 생활습관위험인자를 비교하였다. 분석결과, 비타민 D 수준이 높아질수록 체지방율(p=.030)과 좌식습관(p<.001)은 통계적으로 유의하게 감소하는 선 경향성이 나타났으며, 골격근량(p=.037), 심폐체력(p<.001) 그리고 HDL-C(p=.013)은 통계적으로 유의하게 증가하는 선 경향성이 나타났다. 또한 심폐체력이 낮은 집단이 높은 집단보다 비타민 D 부족 또는 결핍에 노출될 상대적 위험도가 남녀 각각 2.144배(95%CI .469-1.808, p=.042), 1.971배(95%CI .305-1.974, p=.045) 정도 높게 나타났으며, 좌식시간이 가장 많은 집단이 가장 적은 집단보다 비타민 D 부족 또는 결핍에 노출될 상대적 위험도는 여성 사무직 근로자 집단에서만 1.262배(95%CI 0.074-1.527 p=.043) 정도 높게 나타났다. 이와 같이 본 연구에서는 사무직 근로자의 낮은 비타민 D 수준은 낮은 심폐체력 및 장시간의 좌식습관과 관련성이 있는 것으로 나타났다. 따라서 사무직 근로자의 건강증진과 충분한 비타민 D 공급을 위해 건강한 생활습관 변화와 근무 환경 개선이 우선적으로 필요할 것으로 판단된다.

• Journal of Nutrition and Health
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• 제29권5호
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• pp.489-498
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• 1996

In order to investigate the effect of smoking on serum lipid pattern and nutrient intake, the subjects, 134 smokers and 113 nonsmokers, were selected from college men student in Kwangju area. We examined the serum lipid pattern of 15 smokers and 18 non-smokers who agreed to venipunture among the subjects and also examined nutrients intake. The results obtained were as follows ; Mean concentrations of 새심 cholesterol, HDL-cholesterol and LDL-cholesterol were 187$\pm$12.1mg/dl, 41.5$\pm$1.7mg/dl, and 87.3$\pm$7.4mg/dl in smokers and 182.6$\pm$8.2mg/dl, 45.5$\pm$1.5mg/dl, and 96.3$\pm$4.3mg/dl in nonsmokers, respectively. The total energy intake was 2264kcal/day and mean constitutional rationof protein, fat and carbohydrate was 12.7 : 24.8 : 62.5 in smokes and 2287kcal/day, 14.1 :25.4 : 60.5 in nonsmokers, respctively. Mena daily intakes of calcium, iron, vitamin C and vitamin A were 612.2mg, 15.7mg, 48.2mg 2029.4 IU in smokers and 629.4mg, 15.3mg, 68.4mg 2339.1 IU in nonsmokers, respectively. Vitamin C intake was significantly different at p<0.05 between the smokers and nonsmokers. Intakes of cholesterol, SFA and MUFA were not significantly different between the two groups. Mean daily PUFA intake of smokers and nonsmokers were 5.5g and 6.9g respectively. PUFA intake was significantly different at p<0.05 between the two groups. The results of this study showed that smoking have a tendency to raise coronary heart disease occurrence in the respect of serum lipid levels and vitamin C, PUFA intake were significantly different.

• Biomolecules & Therapeutics
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• 제3권4호
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• pp.304-310
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• 1995

This study was done to investigate the effect of vitamin C on hepatic biliary and microsomal function during ischemia and reperfusion. Rats were treated with vitamin C(20, 100, 400, 1600 mg/kg) or with vehicle(saline) and then subjected to 60 min no-flow hepatic ischemia in vivo. Control animals were time-matched sham ischemic animals. After 1 or 5 hr of reperfusion, bile was collected, blood was obtained from the abdominal aorta, and liver microsomes were isolated. In vehicle-treated ischemic rats, serum ALT and AST levels peaked at 5 hr and were significantly attenuated by vitamin C 20 mg/kg and 100 mg/kg treatment. Similarly, hepatic wet weight-to-dry weight ratio was decreased in the vehicle-treated ischemic group. Vitamin C 20 mg/kg and 100 mg/kg treatment minimized the increase in this ratio. Lipid peroxidation was elevated in vehicle-treated ischemic group, but this elevation was also inhibited by vitamin C 20 mg/kg and 100 mg/kg treatment. Bile flow and cholate output, but not bilirubin output, were markedly decreased by ischemia/reperfuzion. Vitamin C 20 mg/kg and 100mg/kg treatment restored the secretion but vitamin C 1600 mg/kg reduced the cholate output. Cytochrome P-450 content was decreased by ischemia/reperfusion and restored by vitamin C 20 mg/kg and 100 mg/kg treatment to the level of sham operated group but decreased by vitamin C 1600 mg/kg. Aminopyrine N-demethylase activity was decreased and aniline p-hydroxylase activity was increased by ischemia/reperfusion. The changes in the activities of aminopyrine were prevented by vitamin C 20 mg/kg and 100 mg/kg treatment, but not by 400 mg/kg and 1600 mg/kg treatment. Our findings suggest that ischemia/reperfusion diminishes hepatic secretory functions as well as microsomal drug metabolizing systems, small doses(20, 100 mg/kg) of vitamin C significantly ameliorates and large doses(400, 1600 mg/kg) of vitamin C aggravated these ischemia/reperfusion-induced changes.

• 핵의학기술
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• 제12권1호
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• pp.62-65
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• 2008

배경: Vitamin $B_{12}$ 및 Folic acid는 모두 수용성 비타민의 일종으로 생체 내에서 보조 효소로서의 작용을 가지고 있지만 특히 세포핵의 DNA 합성에 관여하고 있다. 주로 거대 적아구성 빈혈의 감별 진단과 임신 중 엽산의 저장을 평가하기 위해 측정한다. 용혈 혈청은 혈구 중의 엽산과 비타민 $B_{12}$가 용출하기 때문에 사용할 수 없다고 알려져 있으나 수탁 검사기관인 본원의 특성상 이 내용을 충실히 따르기에는 어려운 경우가 있다. 이에 용혈의 정도가 검사 결과치에 어느 정도 영향을 미치는가에 대해 알아보고자 검사를 시행해 보았다. 방법: 용혈되지 않은 검체를 대조군으로 하고 기계적으로 용혈을 일으켜 용혈 정도에 따라 A, B, C그룹으로 분리하여 용혈 검체를 그룹별 각각 10개씩 준비한다. 경쟁반응 원리인 M사의 Vitamin $B_{12}$ [$^{57}Co$]/Folate [$^{125}I$] 시약으로 검사를 실시한다. 결과: Vitamin $B_{12}$는 검체의 용혈이 결과 값에 큰 영향을 미치지 않았다. 하지만 Folic acid의 경우에는 용혈의 정도에 따라 값이 증가 하였으며, 심하게 용혈 된 경우 모두 표준곡선의 최고치의 값(20 ng/ml 이상)을 확인할 수 있었다. 고찰: 수탁기관인 본원에서는 Vitamin $B_{12}$ 및 Folic acid 검사 의뢰 병원에 검체의 용혈이 검사 결과에 미치는 영향과 검체의 용혈을 막기 위해 채혈 시 주의할 점들을 홍보하고, 분리, 보관 시 세심한 주의를 기울여야 할 것이다. 위의 결과에서 보았듯이 용혈된 검체로 Folic acid검사를 해서는 안 되며 Vitamin $B_{12}$ 또한 결과 값에는 큰 영향을 미치지는 않지만 용혈에 주의할 필요가 있다.

• IMMUNE NETWORK
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• 제3권3호
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• pp.211-218
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• 2003

Background: Vitamin C is an essential nutrient, taken as a daily supplement by many people. Recently, high-dose vitamin C is considered as a therapeutic regimen in some clinical situations. Until now, few studies have been done with the effects of high-dose vitamin C on the immune response. Methods: In this experiment, the effects of high-dose vitamin C on cell-mediated immune response in immunologically competent mice were evaluated. After intraperitoneal injection of 2.5, 5, or 10 mg/day of vitamin C for 10 days, delayed type hypersensitivity (DTH) was provoked against DNFB in the pinnae as a model for cell-mediated immune response. Severity of DTH reaction was evaluated as the thickness of pinnae, and the vitamin C levels were measured in the serum, liver, kidney, lung, pinnae, and splenocytes. Results: After challenge, the thickness increased at its peak on the $2^{nd}$ day in all groups. On the first day, the pinnae were thicker in the injected groups than in the control. On the contrary, the increment of the pinnae thickness was attenuated and the number of cells infiltrated in the site of DTH decreased proportionately to the amount of vitamin C administered from the second day on. With vitamin C exogenously given, the serum level peaked at 30 min after injection, and returned abruptly to its basal level without accumulation. However, it accumulated in the liver, kidney, and especially in the pinnae inflamed and splenopcytes, proportionately to the amount administered. Conclusion: Based on these results, it is suggested that, in one hand, exogenously administered high-dose vitamin C accumulated in the splenocytes and presumably changed the function of them resulting in the augmented cell-mediated immune response, as was revealed in the first day of DTH reaction. On the other hand, it seems likely that the vitamin C also showed anti-inflammatory effects.