• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 학술회의 논문집 정보 및 제어부문 B
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• pp.519-523
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• 2003

This paper describes the communication system of sequencing sorter. Generally sequencing sorter is a machine that sorts mails by delivery order. And designed sequencing sorter is composed of 5 physical modules. So, it is necessary to communicate with each module and to control this communication. A computer called Machine Management Computer, controls this. This paper is about the communication system with MMC and module controllers. This is PC-based, asynchronous full-duplex 4-wire serial systems. 3 protocol layers are presented and stop-and-wait flow control is adopted. Because designed sequencing sorter has to be operated high speed about 27,000 letters per hour, we analyzed the network traffic in the worst case. So, we could find that the communication system has to use above 115,200bps speed.

• 산업공학
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• 제14권3호
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• pp.247-254
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• 2001

As current postal automation is limited to dispatch and arrival sorting, delivery sequence sorting is performed manually by each postman. It not only acts as a bottleneck process in the overall mailing process but is expensive operation. To cope with this problem effectively, delivery sequence sorting automation is required. The important components of delivery sequence sorting automation system are sequence sorter and Hangul OCR which function is to extract the address of delivery point. DSI database will be interfaced to both Hangul OCR and sequence sorter for finding the accurate delivery sequence number and stacker number. The objectives of this research are to develop DSI(Delivery Sequence Information) database prototype and client application for managing information effectively. For database requirements collection and analysis, we draw all possible sorting plans, and apply the AHP(Analytic Hierarchy Process) method to determine the optimal one. And then, we design DSI database schema based on the optimal one and implement it using Oracle RDBMS. In addition, as address information in DIS database consist of hierarchical structure which has its correspondence sequence number, so it is important to reorganize sequence information accurately when address information is inserted, deleted or updated. To increase delivery accuracy, we reflect this point in writing application.

• 한국농촌생활과학회지
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• 제8권2호
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• pp.111-117
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• 1997

In this study we tried to give a decision on propriety of working conditions, to present ideas on reducing work loads. and to grope for efficiency of agricultural works. For this we examined the actual conditions of working environment, farmer's clothes, working posture, working methods, working time, resting state, fatigue recovery methods during cultivating lettuce in the winter greenhouse. And Ive improved harmful factors that affect farmer's health by considering results of previous study and farmer's subjective sensation. And we measured, compared, and analyzed the farmer's work loads before and after improvement. The results of this study are as follows ; 1. According to examine the actual conditions of cultivating lettuce in the winter greenhouse, farmers have experienced physical and mental chronic fatigue on the basis of the hot and humid crops-centered working environment, the rough ground condition, inconsistent arrangement of working stand and sorter, heavy-weared habits. and unsuitable working posture. 2. When we improved harmful factors that affect farmer's health, conformed the positive effects on important work efficiency index such as heart rate, electromyovolume, body temperature, and microclimate inside clothing and work loads were decreased by improving the hot and humid working environment, eliminating the hillock and obstacles of working path. deliver way, arranging the working stand and sorter consistantly, decreasing the clothing weight, improving the working postures and methods as using assistant appliances, alloting the working time and sequence effectively and presenting the light gymnastic exercises and rest for fatigue restoration.

• 한국농촌생활과학회지
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• 제8권2호
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• pp.119-124
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• 1997

In this study we tried to give a decision on propriety of working conditions, to present ideas on reducing work loads, and to grope for efficiency of agricultural works. For this we examined the actual working conditions of cultivating welsh onion in the summer ground. And we improved harmful factors that affect farmer's health by considering results of previous study and farmer's subjective sensation. And we measured. compared, and analyzed the farmer's work loads before and after improvement. The results of this study are as follows ; 1. According to examine the actual working conditions of cultivating welsh onion in the summer ground, farmers have experienced physical and mental chronic fatigue on the basis of farmer's appel to eye - fatigue and sun - burned skin on hot working environment including excessive ultraviolet rays, the rough ground condition, inconsistent arrangement of working stand and sorter, heavy - weared habits, and unsuitable working posture. 2. When we improved harmful factors that affect farmer's health, conformed the effects on important work efficiency index such as heart rate, electromyovolume, body temperature, and microclimate inside clothing and work loads were decreased by eliminating the hillock and obstacles of ground, decreasing the clothing weight, using proper clothing appliances such as hat and sunglasses, controlling height of working stand and sorter suitably, improving the working postures and methods as using assistant appliances, alloting the working time and sequence effectively and presenting the light gymnastic exercises and rest for fatigue restoration.

• IMMUNE NETWORK
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• 제8권4호
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• pp.137-142
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• 2008

Background: CD11c, also known as integrin alpha x, is one of the optimum markers of dendritic cells. However, the regulation of the CD11c expression in mouse has not been identified yet. In this study, in order to analyze the regulation of CD11c expression, the promoter of CD11c was cloned and characterized. Methods: To identify the promoter portion, various sizes of what are considered to be CD11c promoter fragments was amplified by polymerase chain reaction (PCR), using mouse genomic DNA as a template. After sequence was obtained, these fragments were transfected into various cell lines including mouse dendritic cell lines such as JAWSII and DC2.4 and L929 as control cell line.. The promoter activity of three promoter fragments was measured and compared by luciferase activity in the transfected cells. Results: Three clones with size of 1kb, 3kb and 6kb were obtained from mouse genomic DNA. Flow cytometry analysis of JAWSII cells revealed that 52% of the cells expressed CD11c, which was confirmed by RT-PCR analysis. On the contrary, L929 and DC 2.4 cells did not express CD11c. The CD11c+ JAWSII cells were enriched from 52% to 90% with cell sorter. The comparative luciferase activity analyisis demonstrated that the region responsible for tissue specific expression was contained within -3 kb and the clone with size of 3 kb particularly showed higher luciferase activity than 6 kb and 1 kb clones. Conclusion: The CD11c promoter region containing the region responsible for tissue specificity was successfully cloned and -3 kb region showed the highest activity.

• 환경생물
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• 제38권3호
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• pp.433-449
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• 2020

Nine fish and one clam species were collected from freshwater environments in Korea, including four lakes, two streams, and the Nakdong River, to investigate the host-associated bacteria. Hundreds of bacterial strains were isolated from the samples using a cell sorter and a dilution plating method. After identification of the bacterial strains using 16S rRNA gene sequences, 42 strains with greater than 98.7% sequence similarity with validly published species were determined to be unrecorded bacterial species in Korea. These strains were phylogenetically diverse and assigned to four phyla, six classes, 17 orders, 27 families, and 32 genera. At the genus level, the unrecorded species were classified as Corynebacterium, Mycobacterium, Mycolicibacterium, Gordonia, Williamsia, Modestobacter, Brachybacterium, Sanquibacter, Arthrobacter, and Mycolicibacterium of the class Actinobacteria; Empedobacter, and Flavobacterium of the class Flavobacteriia; Fictibacillus, Psychrobacillus, Cohnella, Paenibacillus, Rummeliibacillus, Enterococcus, and Vagococcus of the class Bacilli; Aquamicrobium, Paracoccus, and Sphingomonas of the class Alphaproteobacteria; Achromobacter, Delftia, and Deefgea of the class Betaproteobacteria; and Aeromonas, Providencia, Yersinia, Marinomonas, Acinetobacter, and Pseudomonas of the class Gammaproteobacteria. The 42 unrecorded species were subjected to further taxonomic characterization using gram staining, cellular and colony morphological determination, biochemical analyses, and phylogenetic analyses. This paper provides detailed descriptions of the 42 previously unrecorded bacterial species.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1107-1112
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• 2009

The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sperm, separate sperm into X- and Y-enriched pools, and assess the efficacy of sorting. Sperm collected from Duroc and miniature pigs were stained with 20.8 $\mu{M}$ Hoechst 33342 and analyzed using a high-speed cell sorter. Measurement of the fluorescence intensity of stained sperm nuclei revealed that the X-bearing sperm of Duroc and miniature pigs respectively contain 2.75% and 2.88% more DNA than Y-bearing sperm. In total, 50.18% of the sperm were assigned to the X-sorted sample and 49.82% was assigned to the Y-sorted sample for Duroc pigs. For miniature pigs, the Xsorted sample represented 50.19% of the population and the Y-sorted represented 49.81% of the population. Duplex PCR was used to evaluate accuracy of sorting. A fast and reliable method for porcine sexing was developed through amplification of the sex-determining region of the Y chromosome gene (SRY). Oligonucleotide primers were designed to amplify the conserved porcine SRY high motility group (HMG) box sequence motif. We found that the primer pair designed in this study was 1.46 times more specific than previously reported primers. Thus, this study shows that the present method can be applied in porcine breeding programs to facilitate manipulation of the sex ratio of offspring and to achieve precise sexing of porcine offspring by amplification of the HMG box of the SRY gene.