• Title/Summary/Keyword: separating point

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Biochemical properties of a purified protein in cystic quid of Taenia solium metacestodes (유조낭고충 낭액에서 친화성 크로마토그래피로 분리한 항원 단백질의 생화학적 성상)

  • Cho, Seung-Yull;Kim, Suk-Il;Kang, Shin-Yong;Kong, Yoon
    • Parasites, Hosts and Diseases
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    • v.26 no.2
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    • pp.87-94
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    • 1988
  • By affinity chromatography using a monoclonal antibody as ligand, Kim et at. (1986) purified a protein fraction in cystic fluid of Taenia solium metacestodes (CF) In this study, the biochemical properties of the purified protein were characterized. Discontinuous-polyacrylamide gel electrophoresis (disc-PAGE) of the protein at 4.5∼10% separating gel concentration showed its molecular weight (MW) to be 150 kilodalton (kDa) in non·denatured state, while denaturing sodium dodecyl suifate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that it was composed of 3 different subunits with respective fnw of 15, 10 and 7 kDa. Subunit of 7 kDa was shown to be linked to other subunits by disulade bonds. Isoelectric point of the protein was pH 6.8. The protein was relatively heat-stable for immunologic analysis. These properties indicated that the protein, comprising about 70% of total content in CF, had similar biochemical characters with antigen B of Oriol et at.(1971) in hydatid cyst quid (HF).

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The Applicability of Analysis Scheme for Spatio-Temporal Droughts Using Mass Moment Concept (질량모멘트 개념을 이용한 시공간적 가뭄해석기법의 적용성 분석)

  • Yoo, Ji Young;So, Byung Jin;Kim, Tae Woong;Kwon, Hyun Han
    • Journal of Korea Water Resources Association
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    • v.45 no.10
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    • pp.1069-1079
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    • 2012
  • In this study, to analyze travelling route and transition characteristics which is a spatial time interpretation method now actively progressed in domestic as well as abroad, it was intended to develop new drought interpretation technique which can decide the centroid and orbit of drought through assuming ellipse using Mass Moment concept. First of all, after estimating Standard Precipitation Index (SPI) per different precipitation observatory station to extract drought events, by selecting precipitation sites where drought starting and end point are same, these were categorized as CASE. As a results, with various CASE selections falling in specific duration for monthly drought analysis, it is possible to find out drought area that additionally occurred, and drought reliving process could be confirmed more definitely. Therefore, if the research methods adopted in this study for drought monitoring are utilized, not only accurate spatio-temporal drought analysis is possible, also pattern of drought centroid movement can be analyzed by establishing statistically significant spatial characteristics data after separating all the drought events that occurred sporadically in Korea Peninsula.

A Study on Improvement of the DDHV Estimating Method (설계시간교통량 산정방법 개선)

  • 문미경;장명순;강재수
    • Journal of Korean Society of Transportation
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    • v.21 no.5
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    • pp.61-71
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    • 2003
  • Existent DDHV draws and is calculating K coefficient. D coefficient from sum of traffic volume two-directions time. There is difference of design order and actuality order, error of DDHV estimation value, problem of irregular change etc. of DDHV thereby. In this study, among traffic volume of each other independent two direction(going up, going down), decide design target order in the directional traffic volume, presented way(way) applying without separating K coefficient and D coefficient at the same time. The result were analysis about national highway permanent count point 360 points 30 orders by existing DDHV estimation value method(separation plan) analysis wave and following variation appear. - design order and actuality order are collision at 357 agencies(99.2%) - actuality order special quality : Measuring efficiency of average 80 orders, maximum 1,027 order, minimum 2 orders - error distribution of design order and actuality order : inside 10 hours is(30$\pm$10hour) 106 points(29.4%), 254 points(70.6%) more than 30 orders and $\pm$10 orders error occurrence be - DDHV estimation value : Average 8.4%, maximum 46.7% The other side, average 50 orders. error improvement effect of DDHV 8.4% was analysed that is at design hourly volume computation by inseparability method in case of AADT premises correct thing because inseparability plan agrees actuality order at whole agency with design order and measuring efficiency of DDHV estimation value is "0".t;0".uot;.

Acridine Orange Stained Micronucleus Assay in Human B and T-lymphocytes after Low Dose ${\gamma}-irradiation$ (아크리딘 오렌지 형광염색법을 이용한 저선량 감마선 유도 말초혈액 B와 T-림프구 미소핵 분석)

  • Choi, Jeong-Mi;Kim, Hee-Sun;Yang, Kwang-Hee;Kim, Cha-Soon;Lim, Yong-Khi;Kim, Chong-Soon;Woon, Jae-Ho
    • Journal of Radiation Protection and Research
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    • v.29 no.1
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    • pp.9-15
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    • 2004
  • Firstly, we compared the two staining techniques, Giemsa and Acridine orange, to determine micronuclei on samples of cultures of five healthy human peripheral blood lymphocytes after ${\gamma}-irradiation\;(^{137}Cs)$ in dose ranges of 0 to 800cGy. It was found that the Acridine orange staining method gives more reliable results than the usual Giemsa staining method in micronucleus tests. Moreover, the frequency of micronuclei in cytokinesis-blocked human B-lymphocytes was studied after in vitro irradiation in dose ranges of 0 to 50cGy. After setting and separating the B-lymphocytes, the frequency of radiation-induced micronuclei were observed as the end-point markers for the low-dose radiation dosimetry after staining with Giemsa and Acridine orange dyes. The micronuclei frequency in B-lymphocytes was significantly elevated from 10 to 30cGy ${\gamma}-irradiation$. The determination of micronuclei in B-lymphocytes after staining with Acridine orange was higher than that of Giemsa. The frequency of micronuclei in B-lymphocytes was observed to be at least two times higher than those of T-lymphocytes Giemsa in dose increasing. Therefore, the determination of low-dose radiation-induced micronuclei in B-lymphocytes after staining with Acridine orange is likely to have the greatest potential in the estimation of low dose radiation exposure.

Evaluation of the Bending Behavior of RC beam by Using Color-based Image Processing Method (색상에 기반한 영상분석기법을 이용한 콘크리트 거더의 휨 거동 분석)

  • Woo, Tae-Ryeon;Jung, Chi-Young;Kim, In-Tae;Lee, Jong-Han;Cheung, Jin-Hwan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.4
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    • pp.48-54
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    • 2020
  • Cracks in reinforced concrete structures are the most common type of damage and are used as important analytical data to understand the fracture behavior characteristics of structures. Currently, there is a problem that most of the crack investigation relies on visual inspection, therefore many researchers have proposed image analysis techniques to improve the problem. In this study, we proposed a crack evaluation method to be applied at an indoor experimental level using image analysis method. The image analysis technique using color is for distinguishing a boundary surface between objects existing in an image, and is a method for separating similar colors into one region based on a predefined color. In this study, to improve the accuracy of image analysis, blue paint was applied to the concrete surface and bending experiments were performed. The image analysis method was able to measure the crack width with superior accuracy compared to the crack diameter, and at the same time, it was also possible to analyze the deflection of the beam. Both the crack and deformation were able to confirm the accuracy similar to the existing measurement method, and it was found that the image analysis method was very excellent in terms of applicability.

An Efficient Composite Image Separation by Using Independent Component Analysis Based on Neural Networks (신경망 기반 독립성분분석을 이용한 효율적인 복합영상분리)

  • Cho, Yong-Hyun;Park, Yong-Soo
    • Journal of the Korean Institute of Intelligent Systems
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    • v.12 no.3
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    • pp.210-218
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    • 2002
  • This paper proposes an efficient separation method of the composite images by using independent component analysis(ICA) based on neural networks of the approximate learning algorithm. The Proposed learning algorithm is the fixed point(FP) algorithm based on Secant method which can be approximately computed by only the values of function for estimating the root of objective function for optimizing entropy. The secant method is an alternative of the Newton method which is essential to differentiate the function for estimating the root. It can achieve a superior property of the FP algorithm for ICA due to simplify the composite computation of differential process. The proposed algorithm has been applied to the composite signals and image generated by random mixing matrix in the 4 signal of 500-sample and the 10 images of $512{\times}512-pixel$, respectively The simulation results show that the proposed algorithm has better performance of the learning speed and the separation than those using the conventional algorithm based method. It also solved the training performances depending on initial points setting and the nonrealistic learning time for separating the large size image by using the conventional algorithm.

A study on composition and narrative style of 『Jwagyebudam』 (『좌계부담(左溪裒談)』의 구성과 서술방식)

  • Cho, Jeongyun
    • (The)Study of the Eastern Classic
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    • no.63
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    • pp.83-113
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    • 2016
  • This paper is focused on objective and meaning of its compilation through reviewing system, composition, method of description, and content of "Jwagyebudam". "Jwagyebudam" has all round form of a history and character book by recording via arranging figures from the end of 16th century to the middle of 18th century in a chronological order. Figure were not only assigned at close range by steretyping people into groups to a degree in it, but also recorded in a chronological order. In view of method of description, "Jwagyebudam" concentrated a theme by recording via separating different people associated from a figure and content of the same event by using the same method like 'Hogeonbeom(互見法)' shown in "Sagiyeoljeon(史記列傳)". In addition, it obtained effect to objectify evaluation of figures included. In a method of embodying figures, it has described concretely and in a three dimensions figures by recording anecdotes and poems associated with them via focusing on lifelong characteristic aspect of corresponding figures. In other words, "Jwagyebudam" can be called by biographies or character books, writings specialized in characters center among writings of method to weave freely experience. In a broad sense, it can be called a writing to cover a function of history book, poem and picture. This was located to a starting point of variation of inclusive and descriptive method. Namely, several kinds of writings in late Joseon dynasty can show aspect to fulfill specialized aspect gradually.

A Study on the Necessity of Verification about depot level maintenance plan through the Weapons System cases analysis (무기체계 사례 분석을 통한 창정비개발계획안 검증 필요성 연구)

  • Ahn, Jung-Jun;Kim, Su-Dong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.76-82
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    • 2019
  • This study has done to search for a solution to remove risk limitedly caused by separating weapon system acquisition from operation and maintenance at the view point of Logistic Commander who's responsible for stable operation and maintenance after acquiring weapon system. At the System development stage, unverified overhaul development plan may cause additional manpower and costs after the development, and furthermore it is likely to have risk to lower reliability of the military. Thus, research and development agency should write overhaul development plan at the System development stage, and it should be verified through evaluation and verification test. Secondly, during research and development, institutional supplementation is needed to calculate human and material resources writing overhaul development plan. Thirdly, it should be able to analyze proper operation & maintenance plan and cost for overhaul plan at the pre-investigation stage. Fourthly, the base which can develop overhaul concept and overhaul factors should be included in the need and need determination document. Lastly, for the weapon system which has small amount of high power figure, project management should be performed to be able to specify at the each acquisition level of weapon system to realize Article 28, clause 3 and 4 of Defense business law.

A Study on Smart Job Matching Platform for Personal Assistant of the Disabled (장애인 활동보조인의 구인·구직을 위한 비대면 플랫폼에 관한 연구)

  • Kang, Seungae
    • Convergence Security Journal
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    • v.21 no.3
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    • pp.75-80
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    • 2021
  • This study provided personnel and recruitment information related to job offer and job hunting, and proposed a non-face-to-face platform that can facilitate matching of one-time services as well as periodic services. The existing recruitment and job search method for personal assistant of the disabled is a one-way matching method in which a person in charge of the service providing centers assigns an activity assistant belonging to the institution when a consumer requests an personal assistant service, so the choice of job seekers is very weak. The job matching platform of the activity assistant for the disabled can be logged in separately by dividing users and separating them into the interface of the disabled or the disabled family members (job seekers) and activity assistants for the disabled (job seekers). Search for job offer and job seekers that meet their respective conditions, and real-time bidirectional decision-making through the chat function, and when the activity assistance service is completed, recruiters and job seekers can write service reviews and evaluations from their own point of view. The real-time interactive decision-making matching platform will also help minimize blind spots where it is difficult to provide services when short-term or one-time assistance is needed.

The Origin of Korean Confucianism and Dongyi Culture - Discussion on the Theory of Professor Lew, Seung-Kook (한국유교의 기원과 동이문화(東夷文化) - 류승국 교수의 설을 논함 -)

  • Choi, Young Sung
    • The Journal of Korean Philosophical History
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    • no.57
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    • pp.31-55
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    • 2018
  • Professor Dowon (道原) Lew, Seung-Kook (柳承國: 1923~2011) was a master of Eastern philosophy in Korea, in the 20th century. 'Confucianism' was the root of his discipline. Nevertheless he studied extensively throughout Eastern philosophy. He was also a pioneer of 'Korean Philosophy', who devoted himself to 'establishing the original form (原型) of Korean thought'. Professor Lew showed a unique view on how Confucian thought was formed. He has done many years of precise comparative analysis of the results of archaeological studies before 1970, particularly the study of bone-and-shell inions, and what was said in previous literature. As a result, he concluded that "we cannot discuss the origins of Confucian thought by separating the relationship with the Dongyi tribe". Confucianism was formed in relation to Dongyi. The purpose of his attention to the Dongyi tribe - RenFang tribe (人方族) was not to examine Dongyi tribe and its culture. His purpose was to examine how Confucian thought was formed and to examine the relevance of Dongyi tribe in this process. This was in conjunction with the task of exploring 'the original form of Korean thought', whether he pretended or not. Professor Lew, Seung-Kook's theory differed not only from the conventional view of the academic world in his time but also from the conventional view up to now. It is a pity that it was not yet discussed it in the academic world. I consider that it is necessary to seriously review Professor Lew and Seung-Kook's theory at this point when China has outlined the Northeast project (東北工程).