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Acridine Orange Stained Micronucleus Assay in Human B and T-lymphocytes after Low Dose ${\gamma}-irradiation$  

Choi, Jeong-Mi (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Kim, Hee-Sun (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Yang, Kwang-Hee (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Kim, Cha-Soon (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Lim, Yong-Khi (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Kim, Chong-Soon (Radiation Health Research Institute, Korea Hydro and Nuclear Power Corporation)
Woon, Jae-Ho (National Veterinary Research and Quarantine Service)
Publication Information
Journal of Radiation Protection and Research / v.29, no.1, 2004 , pp. 9-15 More about this Journal
Abstract
Firstly, we compared the two staining techniques, Giemsa and Acridine orange, to determine micronuclei on samples of cultures of five healthy human peripheral blood lymphocytes after ${\gamma}-irradiation\;(^{137}Cs)$ in dose ranges of 0 to 800cGy. It was found that the Acridine orange staining method gives more reliable results than the usual Giemsa staining method in micronucleus tests. Moreover, the frequency of micronuclei in cytokinesis-blocked human B-lymphocytes was studied after in vitro irradiation in dose ranges of 0 to 50cGy. After setting and separating the B-lymphocytes, the frequency of radiation-induced micronuclei were observed as the end-point markers for the low-dose radiation dosimetry after staining with Giemsa and Acridine orange dyes. The micronuclei frequency in B-lymphocytes was significantly elevated from 10 to 30cGy ${\gamma}-irradiation$. The determination of micronuclei in B-lymphocytes after staining with Acridine orange was higher than that of Giemsa. The frequency of micronuclei in B-lymphocytes was observed to be at least two times higher than those of T-lymphocytes Giemsa in dose increasing. Therefore, the determination of low-dose radiation-induced micronuclei in B-lymphocytes after staining with Acridine orange is likely to have the greatest potential in the estimation of low dose radiation exposure.
Keywords
T/B-lymphocytes; acridine orange; Giemsa; low dose radiation; dosimetry;
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