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Characterization of Mud Loach (Misgurnus mizolepis) Apolipoprotein A-I: cDNA Cloning, Molecular Phylogeny and Expression Analysis (미꾸라지(Misgurnus mizolepis) Apolipoprotein A-I cDNA의 구조, 분자계통 및 발현 특징 분석)

  • Lee, Youn-Ho;Noh, Jae-Koo;Kim, Keun-Yong;Cho, Young-Sun;Nam, Yoon-Kwon;Kim, Dong-Soo
    • Journal of Aquaculture
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    • v.20 no.1
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    • pp.65-72
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    • 2007
  • Full length complementary DNA encoding apolipoprotein A-I (apoA-I) was isolated and characterized in mud loach (Misgurnus mizolepis). Mud loach apoA-I cDNA encoding 24 bp of 5'-untranslated region (UTR), 762 bp of single open reading frame (ORF) consists of 254 amino acids and 293 bp of 3'-UTR excluding stop codon and poly (A+) tail. Two overlapping polyadenylation signals (AATAAAATAAA) was found 9 bp prior to the poly (A+) tail. Mud loach apoA-I represented considerable homology to those from other teleost species at amino acid level with conserving common features of vertebrate apoA-I. Molecular phylogenetic analysis inferred the phylogenetic hypothesis that was generally in accordance with the previous taxonomic relationship. Apolipoprotein A-I mRNA was detected in various tissues, but the mRNA levels were quite varied depending on tissues based on semi-quantitative RT-PCR. Liver and brain showed the significantly higher levels of apoA-I transcripts than other tissues. mRNA expression of apoA-I was quite low in very early stage of embryonic development, however dramatically enhanced from 8 hours post fertilization. This increased mRNA level was retained consistently up to 14 days post hatching.

Calmodulin of Olive Flounder Paralichthys olivaceus : Cloning and Expression Analysis

  • Hong, Gyeong-Eun;Kong, Hee Jeong;Nam, Bo-Hye;Kim, Young-Ok;Kim, Woo-Jin;Lee, Sang-Jun;Choi, Tae-Jin
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.4
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    • pp.234-237
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    • 2007
  • Calmodulin (CaM) is a $Ca^{2+}$-binding protein essential for biological functions mediated through $Ca^{2+}$-dependent mechanism. A cDNA clone for CaM was isolated from a cDNA library of olive flounder Paralichthys olivaceus. The CaM cDNA concists of 782 bp and encodes a polypeptide of 149 amino acids with four $Ca^{2+}$-binding motifs EF-hands (EF-I, EF-II, EF-III, and EF-IV). The deduced amino acid sequence of CaM shows 97-100% amino acid sequence identity to other CaM sequences. Semi-quantitative PCR analysis revealed that the CaM transcription was began during early development and the CaM mRNA is expressed highly in brain and intestine, and moderately in kidney, gill, and eye of healthy olive flounder. Taken together, CaM may be necessary for early olive flounder development and that it may have a part in homeostasis.

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Mutual Maturity Process between Client-Therapist in Music and Imagery Session: Therapist's Point of View (음악과 심상 세션에서 내담자-치료사간 상호 성장의 과정: 치료사의 관점에서)

  • Kim, Kyoung Sook
    • Journal of Music and Human Behavior
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    • v.9 no.2
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    • pp.35-52
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    • 2012
  • The purpose of the study was to prospect for mutual maturity between therapist-client in Music and Imagery music therapy session from therapists' points of view. Qualitative data has been collected by in-depth interview of therapist-client mutual maturity process, then analysed with grounded theories. The questions of the study were to ask process of mutual maturity experienced by music and imagery therapist and meaning of the experience itself. Therapists ware to answer the moment of mutual maturity, realization and meaning of mutual maturity process through semi-structured in-depth interview. The four therapists who participated in the interview are certified MIT(Music & Imagery Therapist) by Lisa Summer and currently in training GIM. From the in-depth interview, 81 concepts have been collected and have been divided into 20 categories in open coding, then rearranged into 9 categories in axis coding. To explain music and imagery therapists' maturity process through grounded theory paradigm, as a result, the main phenomenon of experience was 'to deal with counter-transference'. When dealing their counter-transference, therapists experienced their maturity process. Supervision and musical/non-musical self-reflection are also used for interactive intervention medium. So, maturity of therapists leads to knowing and accepting herself and mature therapists are able to help clients mature.

A Qualitative Study on Traditional Korean Medicine Treatment for Child Patients with Night Crying - With a Focus on Descriptions by Main Fosterers - (야제 환아의 한의치료에 대한 질적 연구 - 주양육자의 서술을 중심으로 -)

  • Kim, Hyejin;Jeon, Chaeheun;Kim, Eunji;Kim, Hyunho;Leem, Jungtae;Ryu, Suhyang
    • The Journal of Pediatrics of Korean Medicine
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    • v.32 no.1
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    • pp.44-53
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    • 2018
  • Objectives This study sets out to examine treatment experiences of child patients with night crying based on the traditional Korean medicine. Also, the study traces the process in which clients chose traditional Korean medicine treatment, and proposes a possible future direction of Korean medicine treatment. Methods The investigator conducted a semi-standardized open-ended question interview with two main fosterers of a child who received traditional Korean medicine treatment for night crying, and examined two fosterers' experiences of a child with the traditional Korean medicine treatment. Their interviews were recorded with their facial expressions and acts for analysis. Collected data was analyzed based on the Grounded theory. Results The participants felt a sense of responsibility, worry, and fear for their children with night crying. They had easy access to traditional Korean medicine doctors who were in the community and had positive experiences with traditional Korean medicine treatment. They reported that some people had been forced to use traditional Korean medicine, had misunderstandings about traditional Korean medicine, and had prejudice that night crying is not a disease. They felt there was a room for improvement in terms of the high medical expenses and the absence of a cooperative system between Oriental and Western medicine. Conclusions As the first qualitative study on night crying based on the traditional Korean medicine treatment, this study proposed a future direction for traditional Korean medicine treatment for night crying in the shoes of fosterers. The findings showed that the fosterers chose traditional Korean medicine treatment for night crying child based on their feelings, accessibility and experiences with traditional Korean medicine. Fosterers felt a need for improvement of the general public's perceptions about Korean medicine treatment on night crying, and a cooperative system between Oriental and Western medicine.

Market Efficiency in Real-time : Evidence from the Korea Stock Exchange (한국유가증권시장의 실시간 정보 효율성 검증)

  • Lee, Woo-Baik;Choi, Woo-Suk
    • The Korean Journal of Financial Management
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    • v.26 no.3
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    • pp.103-138
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    • 2009
  • In this article we examine a unique data set of intraday fair disclosure(FD) releases to shed light on market efficiency within the trading day. Specifically, this paper analyze the response of stock prices on fair disclosure disseminated in real-time through KIND(Korea Investor's Network for Disclosure) on Korea stock exchange during the period from January 2003 to September 2004. We find that the prices of stock experiences a statistically and economically significant increase beginning seconds after the fair disclosure is initially announced and lasting approximately two minutes. The stock price responds more strongly to fair disclosure on smaller firm but the response to fair disclosure on the largest firm stock is more gradual, lasting five minutes. We also examine the profitability of a short-term trading strategy based on dissemination of fair disclosure. After controlling for trading costs we find that trader who execute a trade following initial disclosure generate negative profits, but trader buying stock before initial disclosure realize statistically significant positive profit after two minute of disclosure. Summarizing overall results, our evidence supports that security prices on Korea stock exchange reflects all available information within two minutes and the Korea stock market is semi-strongly efficient enough that a trader cannot generate profits based on widely disseminated news unless he acts almost immediately.

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A Study on Nurses한 and Patients한 Perceptions of Psychotropic Medication (향정신성 약물치료에 대한 간호사와 환자의 지각 비교 연구)

  • 이평숙
    • Journal of Korean Academy of Nursing
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    • v.24 no.1
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    • pp.47-57
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    • 1994
  • The purpose of this study was to examine nurses’ perceptions of medication treatment for psychiatric patients and to compare these perceptions with the perceptions held by the patients. The methodology used in this study was a descriptive design with semi-structured and open-ended interviews. This study used a convenience sample of 112 nurses who worked in, and 209 patients who were under psychiatric treatment, in four hospitals attached to a university and one national mental hospital in the city of Seoul. The collected data were analyzed by SAS, using percentages for descriptive purposes, and t-test or x$^2$ for comparing the variables. The results were as follows : 1. There was no significant differences between nurses’ and patients’ perceptions on the extent to which patients complied with their medication treatment. Generally speaking, the mean compliance scores for both nurses and patients was high(nurse : (equation omitted)=3.70, Patient : (equation omitted)=3.76). 2. There was a significant difference in nurses’ and patients’ perceptions on the reasons why patients do not take medication. The nurse group indicated that the patients did not take medication because of the “worry about side effects or habituation(49.53%)”, “boredom from long-term use of medication(26.17%)” and “distrust toward medical staff(12.15% )”, but the patient group indicated that they “did not want to be dependent on medication (25%)”, “forgot to take medication(19.7%) and “worried about side effects or habituation(15.91%). 3. As for the necessity of medication, both groups showed some different responses. Even though both groups were aware of the necessity of taking medication, the patient group(21.53%) showed a more negative response. As (or the effects of medication, both groups (nurses and patients ) showed positive responses. However, the nurse group showed a higher positive response (91.07% ) than the patient group(74.16%), 5. Both the patient and nurse group indicated that the most helpful element for the patient’s life under psychiatric treatment was interviews and conversations with therapists and nurses. However, the nurse group showed a higher response(70.15%) than the patients group(47.15%). According to the patient group, family support for the patient was another important factor for psychiatric treatment and daily struggles. In conclusion, as there were differences between the perception of nurses and patients, the nurse must consider the patients’ subjective perceptions first. They should also revaluate their false belief and prejudice concerning the patients’ perceptions. Such information can provide a base to be applied by the nurses in devloping effective mutual relationships with patients which can in turn help in compliance with medication regimen. As it was confirmed that medication was the most important factor in the patients’ recovery, a thorough education program on the therapeutic effect of medication and the necessity of their continued use after discharge is also needed.

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A Usability Testing on the Tablet PC-based Korean High-tech AAC Software (태블릿 PC 기반 한국형 하이테크 AAC 소프트웨어의 사용성 평가)

  • Lee, Heeyeon;Hong, Ki-Hyung
    • Journal of the HCI Society of Korea
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    • v.7 no.2
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    • pp.35-42
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    • 2012
  • The purpose of this study was to evaluate the usability of the tablet PC-based Korean high-tech AAC(Augmentative Alternative Communication System) software. In order to develop an AAC software which is appropriate to Korean cultural/linguistic contexts and communication needs of the users, we examined the necessity and ease of use for the communication functions that are required in native Korean communication, such as polite expressions, tense expressions, negative expressions, subject-verb auto-matching, and automatic sentence generation functions, using a scenario-based user testing. We also investigated the users' needs, preferences, and satisfaction for the tablet PC-based Korean high tech AAC using a semi-structured and open questionnaires. The participants of this study were 9 special education teachers, 6 speech therapists, and 6 parents whose children had communication disabilities. The results of the usability testing of the tablet PC-based Korean high-tech AAC software presented positive responses in general, by indicating overall scores of above 4 out of 5 except in tense and negative expressions. The necessity and ease of use in the tense and negative expressions were evaluated relatively low, and it might be related to the inconsistent interface with the polite expressions. In terms of the user interface(UI), there were users' needs for clear visual feedback in the symbol selection and display, consistent interface for all functions, more natural subject-verb auto-matching, and spacing in the text within symbols. The results of the usability testing and users' feedback might serve as a guideline to compensate and improve the function and UI of the existing AAC software.

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Oriental Nursing Activity Perceived by Nurses, Doctors, and Patients in an Oriental Hospital (한방병원 간호사, 의사, 환자가 지각하는 한방간호업무)

  • Kang, Hyun-Sook;Kim, Won-Ock;Lee, Jung-Min
    • Journal of East-West Nursing Research
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    • v.8 no.1
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    • pp.41-49
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    • 2003
  • Introduction: The purpose of this study was to compare the different concepts of oriental nursing as perceived by nurses, doctors, and patients in oriental hospitals. Method: A descriptive survey design was used for this study. Fifty-eight nurses, 26 doctors and 28 inpatients in 3 oriental hospitals were recruited from October to December 2002. The data were collected through a semi-structured open questionnaire. The data were analyzed by content analysis. Result: 1. The perceived concept of oriental nursing varied among the subjects. The nurses regard it as "a traditional nursing activity based on oriental philosophy" (60.3%), while the doctors viewed it as "a supplement to western nursing activity"(38.5%). For patients, the oriental nursing activity was considered as "a cordial form of nursing like that from a family member" (50.0%) and "an activity that doesn't differ so much from western nursing" (42.8%). 2. As for oriental nursing activities actually practiced, both the nurses and the doctors agreed that they carry out traditional oriental nursing activities such as explaining the treatment (taking oriental medicines, administering acupuncture etc.; nurses 96.6%, doctors 57.7%) and direct care like removing acupuncture needles (nurses 43.1%, doctors 34.6%). Patients replied that a western nursing activity is performed rather than an oriental nursing activity. 3. As for the required oriental nursing activity, nurses stated they apply traditional oriental methods such as CHUNA exercise therapy, moxibustion, cupping method etc. in their nursing practice. Doctors remarked that they try to understand the patients' state by approaching patients through an oriental way of thinking. Patients wish to get kindly care. 4. As a whole, 34.5% of nurses and 25.0% of patients have experienced little satisfaction from oriental nursing activity. Nurses found it valuable to carry out western nursing (39.3%), while patients found satisfaction in the use of a cordial attitude (39.9%). 5. Both nurses and doctors defined the first reason that oriental nursing activity cannot be performed more often was the lack of education in oriental nursing, and the absence of interest in oriental nursing. Conclusion: Nurses and doctors appreciate an oriental way of nursing, and think that this type of nursing activity has been carried out even though it may be infrequent. However this oriental nursing activity has won recognition from patients who view western nursing activity as being more important.

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A Study on Korean Gender Egalitarianism among High School Students (고등학생의 남녀평등의식에 관한 연구)

  • Kim Ji Yoon;Ahn Chae Soon
    • Child Health Nursing Research
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    • v.6 no.3
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    • pp.329-343
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    • 2000
  • This study was conducted to measure the degree of Korean Gender Egalitarianism among the students from six different high schools(132 girls, 127 boys) in Sunchon si, Chunnam province from Dec. 7th to Dec. 15th in 1999. The survey on gender egalitarianism was done in relation to the public understanding of the subject matter, parents' hug times, and parent's communication skills. Korean gender egalitari- anism scale was standardized by Korea Woman Developmental Institutes in 1999. The data was analyzed by SAS program. Results were as follows: 1) Generally, girls are more conscious of gender egalitariannism than boys(t=-13.79, p<.001). Above all, the younger the students are, the more they feel equal(F=8.01, p<.01). In sibling cases, two sisters have the highest level of gender egalitarianism while two brothers have the lowest level of gender egalitarianism(F=9.20, p<.001). Students who come from religious families are more inclined to demonstrate gender equality(t= 2.42, p<.05). However, there is no difference in relation to the parents' ages or academic background or harmony within the family. 2) There is no difference that girls and boys generally communicate with their parents. In ages, 17 years old students communicate more than 18, 19 with parents(father F=3.97 p<.05ㆍmother F=5.31, p<.01). That is, the younger students can communicate with their mothers more often than the older ones. In sibling cases, two sisters have more openly communication to parents while two brothers have(F=2.84, p<.05). Students communicate with their parents regardless of parents' academic background. But those from the family in harmony have more open chance to communicate than those who are not in hamony(father F=21.66, p<.001ㆍmother F=13.37, p<.001). They can comm -unicate with their parents better when their fathers hug them than when they didn't get hugs(father t=-4.76, p<.001ㆍ mother t= -2.34, p<.05). They can also communicate with their patents better when their mothers hug them once or more than when they didn't get hugs(father t=-3.78, p<.001ㆍmother t=-5.64, p<.001). 3) There is no difference whether the father hugs or not, but the mother's hug is very important because students whose mothers hug them once or more have significantly more gender egalitarianism than those students whose mothers didn't do(t=-3.98, p<.001). Based upon these results, it is recommended that we reconsider the role of gender in the socialization of high school students and also include gender egalitarianism in sex education. In this context, further study should be done for the improvement of students' communication within the framework of egalitarianism based upon parents' hugs(semi-language). Continued study is especially important in regards to the mother's hug and its enhancement of the students' gender egalitarianism.

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Development of Success Attribution Scale for Body Guard in Korea (한국 경호원의 성공귀인 측정도구 개발)

  • Kim, Sang Jin
    • Convergence Security Journal
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    • v.17 no.2
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    • pp.173-183
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    • 2017
  • This study is for presenting the factors about success attribution that fit the korean security service conditions and developing the actual analysis of measurement tools by investigating the success factors of the security service agents. To this end, conducted a meeting from the fully open questionnaire at first, and then formed the semi-structured questionnaire, finally carried out the survey from the closed questionnaire and analyzed data from SPSS 21.0, AMOS 21.0 and developed the measurements. It was conducted from December, 2014 to June, 2015. This survey was conducted of 170 security guards after the verification of the content validity though the pilot survey and presented the success attribution factors and standards on the basis of the result form this survey. At the first and second analysis process, the success attribution factors of the guards consisted of inner qualities, external qualities, induction factors for inner qualities, and induction factor for external qualities. On analysis of the final data and documents, however, at the third analysis, the success attribution factors of the guards consisted of 'martial arts and physical ability'(4 questions), 'attitudes to work'(3 questions), 'support for the working environment'(3 questions), 'organized business skills'(3 questions), 'attitudes to the duty'(3 questions), and 'coping with crises(2 questions).