• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3269-3273
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• 2012

In this study, we report a new method for the high contrast imaging of biomolecular interactions at roller printed protein surfaces using thermotropic liquid crystals (LCs). Avidin was roller printed and covalently immobilized onto the obliquely deposited gold surface that was decorated with carboxylic acid-terminated self-assembled monolayers (SAMs). The optical response of LCs on the roller printed film of avidin contrasted sharply with that on the obliquely deposited gold surface. The binding of biotin-peroxidase to the roller printed avidin was then investigated on the obliquely deposited gold substrate. LCs exhibited a non-uniform and random orientation on the roller printed area decorated with the complex of avidin and biotin-peroxidase, while LCs displayed a uniform and planar orientation on the area without roller printed proteins. The orientational transition of LCs from uniform to non-uniform state was triggered by the erasion of nanometer-scale topographies on the roller printed surface after the binding of biotin-peroxidase to the surface-immobilized avidin. The specific binding events of protein-receptor interactions were also confirmed by atomic force microscopy and ellipsometry. These results demonstrate that the roller printing of proteins on obliquely deposited gold substrates could provide a high contrast signal for imaging biomolecular interactions using LC-based sensors.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2003년도 추계학술대회 논문집
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• pp.81-82
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• 2003

We have developed the method for the conjugation of biotinylated DNA to streptavidin-coated QDs. QD-DNA conjugates and a high-sensitive fluorescence imaging technique are adopted to visualize gene transport across the membrane of the live cell in real time. Endocytotic cellular uptake of oligonucleotide and electrically-mediated plasmid DNA transfer into the live cell are monitored by a quantitative microscopic imaging system. Long-term kinetic study enables us to reveal the unknown mechanisms and rate-limiting steps of extracellular and intracellular transport of biomolecules. We designed experimental protocols to conjugate the oligonucleotide or the plasmid DNA to commercially available streptavidin-coated QDs. Gel electrophoresis is used to verify the effect of incubation time and the molar ratio of QDs and DNA on the conjugation efficiency. It is possible to fractionate the QD-DNA conjugates according to the DNA concentration and obtain the purified conjugates by a gel extraction technique.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2006년도 공동학술대회 논문집
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• pp.109-112
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• 2006

기존 상용 전기탐사장비의 한계는 간척지 갯벌 등 해성퇴적층 하부지반조사 적용이 매우 힘들거나, 불가능한 것으로 인지되어 왔다. 최근 전 세계적으로 해저면 또는 연안지역환경 전기탐사 필요성이 대두됨에 따라 자체개발 발전기 전원 5 Ampere 대전류 송신 전기탐사 현장적용이 광양만에서 확인된다. 항만관련 기반시설 연안투자는 지속 확대될 것으로 기존 탐사방법 지반조사에서 탈피하여 항만 교량, 해저터널, 해저 가스 층 등의 효과적 적용 가능성이 기대된다. 한편 조석변화를 고려한 대전류 전기 비저항탐사를 수위별로 시간대 측정하여 연약구역 효과를 비교 시도하였다. 대전류 송신은 탐사전선저항 길이를 고려한 덜 무거운 케이블 선택 설계가 현장야업 시 중요하다.

• 한국치위생학회지
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• 제16권2호
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• pp.205-214
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• 2016

Objectives: The purpose of the study is to investigate the current status and radiographic safety management in Korea. Methods: A self-reported questionnaire was completed by 200 dental hygienists in Jeonbuk province from September 1, 2014 and October 31, 2014. The questionnaire consisted of general characteristics of the subjects, radiation knowledge, radiation safety management, and radiation exposure anxiety. Data were analyzed using SPSS 18.0 program. Results: Duration of clinical experience(r=0.142) and number of daily radiation shot(r=0.145) showed a positive correlation to radiation safety management, and a nrgative correlation to use of intraoral films and digital devices(r=-0.587). A logistic regression analysis was performed in order to evaluate the influence on radiography knowledge. The results showed that the factors had significant influences on the age group over 41 years old(OR 7.25; 95% CI 1.30-40.43) and those who took a position above team leader(OR 0.23; 95% CI 0.59-0.90). Conclusions: It is very important to have the safety management toward dental intraoral imaging and radiograpgic shot in the dental hygienists. Continuous efforts should be emphasized on radiographic safety management and behavior.

• 대한디지털의료영상학회논문지
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• 제11권2호
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• pp.59-62
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• 2009

The purpose of this study is to know about solution of insecurity sense for solving the tediousness and inducing comfortableness in long time MRI examination. Studies were examined with total of 117 patients that the intention expression of self is possible for a month without dividing man and woman in november 2006. Examination methods were filled in questionnaire after testing using Siemens Impact 1.0T. The musical art were selected through intranet search and record sale inquiry using CD player and speaker. That kind of a music were selected with Korean classical music's 10 chapter, pop song 10's chapter, classic's 10 chapter and Korean popular song's 10 chapter. With analytical method, patients of under 40 year-old, or older and patients using ear-pad and head-phone were classified into groups. As analysis result with question item of questionnaire, most of the patients were showed a favor(36.72%) in playing a music, specially, examination of about 15.38% which uses the headphone prefer a music as older generation of over 40 year-old. In conclusion, with installing of the professional musical program in MRI test rooms, if listening to the music with selection by oneself before testing by season, age, time through development of appropriate musical program will be able to expect the more effect.

• 대한수의학회지
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• 제45권3호
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• pp.423-427
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• 2005

Color Doppler imaging (CDI) was carried out to evaluate the anesthetics effect on ophthalmic circulation using CDI-derived resistive index (RI) values. CDI was preformed on 24 dogs, and RI values were calculated for the medial long posterior ciliary artery (mLPCA) and ophthalmic artery (OA) before and after administration of anesthetics. After administration of benoxinate or acepromazine, a significant change of the mLPCA RI was not found. But, a significant decrease of the RI following ketamine (p < 0.001) or xylazine (p < 0.01) administration could be observed as compared with the self-control. Mean RI value of OA also showed this same trend. Intraocular pressure was significantly decrease following benoxinate (p < 0.01), acepromazine (p < 0.01), and xylazine (p < 0.001) administration within normal range. The results suggest that some anesthetics influence on ophthalmic vascular resistance. Therefore, chemical restraint was carefully used in clinical application of CDI-derived RI measurement. Particularly, benoxinate and acepromazine is useful chemical restraint without a change of the ophthalmic vascular resistance.

• Current Optics and Photonics
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• 제7권4호
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• pp.419-427
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• 2023

The structural design of an optical-telescope assembly (OTA) for satellite laser ranging (SLR) is conducted in two steps. First, the results of a parametric study of the major design variables (e.g. dimension and shape) of the OTA part are explained, and the detailed structural design of the OTA is derived, considering the design requirements. Among the structural-shape concepts of various OTAs, the Serrurier truss concept is selected in this study, and the collimation of the telescope according to the design variables is extensively discussed. After generating finite-element models for different structural shapes, self-gravity analyses are performed. To minimize the deflection and tilt of the mirror and frame for the OTA under the limited design requirements, a parametric study is conducted according to design variables such as the shapes of the upper and lower struts and the spider vane. The structural features found in the parametric study are described. Finally, the OTA structure is designed in detail to maintain the optical alignment by balancing the gravity deflections of the upper and lower trusses using the optimal combination of the parameters. Additionally, thermal analysis of the optical telescope design is evaluated.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 추계학술대회
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• pp.124-127
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• 1995

Due to the self-diffusion of nuclear spins, the edge of phantoms is enhanced in the magnetic resonance imaging (MRI), especially in the case of microscopy [1]. According to several published works, theory has been established that the edge enhancement is caused by the motion narrowing around bounded regions due to diffusions of nuclear spins during data acquisition. It is found, however, that the signal decreases due to the diffusion attenuation and image is distorted as edge of the image is sharpened. In this paper, we wilt investigate this signal loss during data acquisition and its effects on image, i.e., image edge enhancement due to the diffusion phenomenon. This result is new and different from the previously discussed edge enhancement due to the diffusion, namely, by motion narrowing effect or spin bouncing effect at the boundary.

• ETRI Journal
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• 제46권3호
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• pp.513-525
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• 2024

Deep neural networks trained on labeled medical data face major challenges owing to the economic costs of data acquisition through expensive medical imaging devices, expert labor for data annotation, and large datasets to achieve optimal model performance. The heterogeneity of diseases, such as Alzheimer's disease, further complicates deep learning because the test cases may substantially differ from the training data, possibly increasing the rate of false positives. We propose a reconstruction-based self-supervised anomaly detection model to overcome these challenges. It has a dual-subnetwork encoder that enhances feature encoding augmented by skip connections to the decoder for improving the gradient flow. The novel encoder captures local and global features to improve image reconstruction. In addition, we introduce an entropy-based image conversion method. Extensive evaluations show that the proposed model outperforms benchmark models in anomaly detection and classification using an encoder. The supervised and unsupervised models show improved performances when trained with data preprocessed using the proposed image conversion method.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2519-2526
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• 2010

Silane-based self-assembly was employed for the surface modification of carbon-coated Si electrodes and their surface chemistry and electrochemical performance in battery electrolyte depending on the molecular structure of silanes was studied. IR spectroscopic analyses revealed that siloxane formed from silane-based self-assembly possessed Si-O-Si network on the electrode surface and high surface coverage siloxane induced the formation of a stable solid-electrolyte interphase (SEI) layer that was mainly composed of organic compounds with alkyl and carboxylate metal salt functionalities, and PF-containing inorganic species. Scanning electron microscopy imaging showed that particle cracking were effectively reduced on the carbon-coated Si when having high coverage siloxane and thickened SEI layer, delivering > 1480 mAh/g over 200 cycles with enhanced capacity retention 74% of the maximum discharge capacity, in contrast to a rapid capacity fade with low coverage siloxane.