• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2010년도 추계 학술논문요약집
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• pp.149-150
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• 2010

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.91-96
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• 1994

This study was conducted to determine the effect of inorganic selenium (Se) sources on rumen bacterial yield, ruminal volatile fatty acid (VFA) production and total tract digestion of timothy hay (Phlewm pratense L.) in Japanese Corriedale wethers. A $3{\times}3$ Latin square design was used with three wethers, three periods and three treatments. In each period, there was 7 d dietary adjustment followed by 5 d total collection of urine and feces. Ruminal fluid samples were obtained at 0, 1, 3, 5 and 7 h postprandially on the final day of the collection period. The three dietary treatments were: (1) without Se supplementation (control); (2) with Se supplementation as sodium selenate; and (3) sodium selenite at a rate 0.2 mg Se/kg dietary DM. The basal diet was timothy hay fed at 2% of body weight/d. Results indicated that there was slight decrease in rumen bacterial yield of animal supplement with inorganic Se, however, differences over the control were insignificant. It was found that Se content of ruminal fluid was negatively correlated (p < 0.05) to rumen bacterial yield. The various VFA contents and acetate and propionate ratio of the different ruminal fluid samples were insignificant across treatment means and the same manner was observed to the different digestibilities (DM, OM, CP, NDF, ADF and NDS). This study concludes that Se supplementation at 0.2 mg Se/kg dietary DM either from sodium selenate or sodium selenite could not significantly influence rumen bacterial functions.

• 한국환경농학회지
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• 제37권2호
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• pp.146-149
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• 2018

아셀렌산염(selenite, ${SeO_3}^{2-}$)을 환원시키는 세균 SE4-1을 복합 산업단지 내 위치한 오염된 하천 퇴적토로부터 분리하였고, 계통분석결과 이 균주는 Citrobacter freundii와 가장 유사하였다. 이 균주는 혐기적 환경에서 아셀렌산염을 원소상 셀레늄(elemental selenium, $Se^0$)으로 환원시켰고, 그 형상은 구형으로 확인되었다. 이러한 고체상의 침전은 셀레늄 산소음이온의 이동성을 줄이고 생물가용성을 줄여, 생태계 독성을 줄이는 잠재성을 제시해 줄 수 있을 것이다.

• 생물환경조절학회지
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• 제15권4호
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• pp.346-351
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• 2006

토양에 비료 형태로 투여한 셀레늄 농도와 처리기간이 마늘의 생육에 미치는 영향을 구명하고자 본 실험을 수행하였다. 셀레늄의 농도가 높고 처리기간이 길수록 생체중은 대조구에 비하여 감소하였다. 셀레늄의 처리농도가 높고 처리기간이 길수록 3주 후 셀레늄의 함량은 구보다는 엽초에 많이 축적되었다 그러나 처리 6주 후에는 구>엽초>뿌리 순으로 셀레늄의 함량이 높았다. 셀레늄 처리 후 토양에 잔류하고 있는 셀레늄의 함량은 대조구와 차이가 없었다.

• Preventive Nutrition and Food Science
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• 제14권2호
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• pp.142-147
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• 2009

In this study, the selenium (Se) accumulations of soybean sprouts and mungbean sprouts treated with various concentrations of Se-solutions were evaluated, as part of a broader effort to produce Se-enriched variants of the plants. Four levels of sodium selenate ($Na_{2}SeO_{4}$)-dissolved solutions (i.e. 0, T0; 6, T1; 60, T2; and $600{\mu}g/mL$, T3) were prepared and sprayed onto the plants during cultivation. The effect of different spraying periods on Se accumulation was also assessed by watering plant groups once a day for periods of one, two, or three days. Se solution remaining on the surfaces of the plants was washed out by spraying with distilled water on the final day of cultivation. However, the increase of Se accumulation in the plants was found to depend on both Se-concentration and watering period, and the soybean sprouts were determined to accumulate Se more effectively than the mungbean sprouts. Additionally, with regard to Se accumulation in the plants, the period of application of Se solution was determined to be more important than the concentration of the Se solution applied. The averaged total levels of Se-enrichment in whole soybean sprouts at T0, T1, T2, and T3 were 0.26, 65.86, 179.62, and $525.12{\mu}g/dry$ matter (DM) g, respectively, and the relative equations relating Se enrichment in soybean sprouts (Y) against watering days (X) were Y=32.505X-36.17 (T1), Y=88.46X-92.04 (T2), and Y=251.11X-254.9(T3). The averaged total levels of Se-enrichment in the whole mungbean sprouts at T1, T2, and T3 group were 0.05, 3.64, and $101.43{\mu}g/DM$ g, respectively, and the relative equations relating Se enrichment (Y) to watering days (X) for mungbean sprouts were Y=1.67X-1.3467 at T1 and Y=48.035X-46.907 at T2. The results of this study suggest that soybean sprouts and mungbean sprouts enriched with bioavailable Se can be produced on a large scale by Se supplementation, allowing for the development of healthy functional foods such as Se-enriched mungbean sprout soups and salads, Se-enriched functional drink and food additives, and selenium tablets to promote health.

• 대한화학회지
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• 제63권5호
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• pp.335-341
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• 2019

배양한 셀레늄 강화 스피룰리나를 ICP-MS에서 동위원소희석법을 이용하여 총농도를 정량하였고, HPLC-ICP/MS를 이용하여 낮은 분자량의 셀레늄 화학종들을 각각 분리하고 정량하였다. 셀레노 화합물들은 일차적으로 증류수를 이용하여 추출하였고 이차적으로는 단백질 분해 효소 protease XIV를 이용하여 단백질들을 분해한 뒤에 추출하였다. 배양한 스피룰리나의 셀레늄 총량은 $414.9{\pm}4.0mg\;g^{-1}$으로 나타났고 증류수로 추출하였을때, 분리한 상등액과 가라앉은 residue는 각각 $318.3mg\;g^{-1}$과 $90.9mg\;g^{-1}$으로서 총량의 약 77%와 22%로 분포되어 있었다. 셀레늄 화학종의 분포는 상등액에서는 주로 selenate ($222.7mg\;g^{-1}$)가 존재하였고 단백질 가수분해를 시키고 조사한 결과, 유기셀레늄들 SeMet ($12.13mg\;g^{-1}$)과 SeCys ($15.20mg\;g^{-1}$)이 추가로 나타났다. Residue에서는 무기 셀레늄이 거의 검출되지 않았고 SeCys과 SeMet이 나타났으며 단백질 분해효소를 사용하여 추출한 경우, 더 많은 셀레노 아미노산들, SeCys ($9.35mg\;g^{-1}$)과 SeMet ($18.23mg\;g^{-1}$), 및 MeSeCys ($1.5mg\;g^{-1}$)이 검출되었다. 무기셀레늄은 주로 스피룰리나의 표면에 흡착된 것으로 보이며 증류수 추출로 이들을 충분히 제거할 수 있었고 결과, residue에서는 대부분 유기셀레늄(99% 이상)만 존재하였다.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1041-1046
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• 2006

Selenium-containing peptide (selenium peptide) was produced by autolysis of total proteins of Saccharomyces cerevisiae grown with inorganic selenium. Selenium peptide exhibited antioxidant activity as a glutathione peroxidase (GPx) mimic, and its activity was dependent on the hydrolysis methods. The GPx-like activity of the hydrolyzed selenium peptide increased 2.7-folds when digested by protease, but decreased by acid hydrolysis. During the autolysis of the yeast cell, the GPx-like activity and selenium content increased 4.3- and 2.3-folds, respectively, whereas the average molecular weight (MW) of selenium peptide decreased 70%. The GPx-like activity was dependent on the MW of selenium peptide and was the highest (220 U/mg protein) at 9,500 dalton. The maximum GPx-like activity (28,600 U/g cell) was obtained by 48 h of autolysis of the cells, which were precultured with 20 ppm of selenate. Selenium peptide showed little toxicity, compared with highly toxic inorganic selenium. These results show the potential of selenium peptide as a nontoxic antioxidant that can be produced by simple autolysis of yeast cells.

• Asian-Australasian Journal of Animal Sciences
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• 제16권3호
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• pp.435-444
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• 2003

In 1957, Schwarz and Foltz discovered that selenium (Se) was an essential trace mineral and nutritionists then started extensive studies to figure out the metabolic function of this element which has been called as toxic mineral. The discovery that glutathione peroxidase (GSH-Px) contained Se demonstrated a biochemical role for Se as an essential trace element. The major physiological function of Se containing GSH-Px is thought to maintain low levels of $H_2O_2$ and other hydroperoxides in the cell to prevent tissues from peroxidation damages. It is known that the GSH-Px activity is increased when animals were fed high dietary levels of Se. Chemical properties of Se have much in common with sulfur (S) therefore Se would follow the sulfur pathways in its metabolism in animal body. Two sources of Se are available for supplementation of Se in animal feed. Inorganic Se can also exist in selenide (-2), elemental (0), selenite (+4) and selenate (+6) oxidation state with other minerals. When sulfur in S containing amino acids is replaced by Se, organic Se can be made and named "eleno"prior to the name of S containing amino acid, i.e. selenomethionine. Selenium deficiency affects humans as well as animals and dysfunctions such as exudative diathesis, retained placenta, mastitis, liver necrosis, Keshan disease, numerous diseases and cancer. From several centuries ago, Se toxicity was recognized in various animal species and much of the current toxic Se levels has been established largely based upon the controlled toxicity studies used inorganic Se. Toxic effects of Se in animal result in reduced feed intake, growth retardation, ataxia, diarrhea, alopecia and sloughing of hooves. However, several experiments demonstrated that Se deficiencies or toxicities were varied by dietary Se levels and sources. Recent studies demonstrated that the incidence of colorectal and prostate cancer was reduced by approximately 50% when humans consumed 200 ${\mu}g$ of Se daily.

• 생물환경조절학회지
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• 제9권4호
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• pp.193-200
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• 2000

본 실험은 바실의 양액재배시 기능성 원소 selenium(Se)을 처리하였을 때 basil의 저장중 품질 변화를 조사하기 위하여 수행되었다. European Vegetable R ' B center에서 개발한 허브 배양액 1배액을 사용하여 담액 순환식으로 재배하였으며, Se을 sodium selenate($Na_2$Se $O_4$)의 형태로 수확 3주전에 2, 4mg. $L^{-1}$를 양액 내 처리하였다. 1$0^{\circ}C$에서 40um ceramic film로 PET 상자를 사용하여 MA저장을 15일간 하였다. 바실의 수경재배시 기능성 물질의 첨가가 저장중 품질 변화에 미치는 영향은 다음과 같다. Se 함량은 양액내 처리 농도에 따라 증가하였고 저장 후에도 같은 경향을 나타내었다. 포장재에 따른 휘발량의 차이는 크지않았다. 엽록소와 정유함량은 Se처리에 의해 증가되었다. 정유 함량은 $Na_2$Se $O_4$.4mg. $L^{-1}$ 처리가 저장후 감소율이 가장 적었다. Se 함량은 2mg. $L^{-1}$처리시 저장전 함량이 112.73ug. $g^{-1}$DM이었고 저장중 감소량은 50%정도였다. 저장중 품질유지와 소비자가 안전하게 Se를 섭취할 수 있는 적정 농도는 2mg. $L^{-1}$이며, 40um ceramic film을 이용한 MA저장이 효과적이었다. 이상에서 바실의 양액재배시 Se처리는 작물의 품질 증진 및 저장중 품질 유지에 효과가 인정되었다. 또 Se 휘발에 의한 정유성분의 변화는 나타나지 않았다. 또 Se 휘발에 의한 정유성분의 변화는 나타나지 않았다.