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http://dx.doi.org/10.5338/KJEA.2018.37.2.20

Selenite Reduction to Elemental Selenium by Citrobacter Strain SE4-1 Isolated from a Stream Sediment  

Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Chonbuk National University)
Cho, Ahyeon (Department of Bioenvironmental Chemistry, Chonbuk National University)
Lee, Hyeri (Department of Bioenvironmental Chemistry, Chonbuk National University)
Publication Information
Korean Journal of Environmental Agriculture / v.37, no.2, 2018 , pp. 146-149 More about this Journal
Abstract
BACKGROUND: Selenium is an essential element for all life forms but can be toxic above certain narrow levels. Prevalent forms of selenium in oxic environment are selenium oxyanions such as selenite and selenate, which may be contaminants in soils and water bodies. Bacterial reduction of more mobile selenium species (selenite or selenate) to less mobile elemental selenium may suggest a benign solution for alleviating toxicity and bioavailability of the selenium species. METHODS AND RESULTS: A facultative anaerobic bacterium, Citrobacter strain SE4-1 was isolated from the contaminated stream sediments and found to effectively reduce selenite to elemental selenium. Aqueous phase of selenite was analyzed by inductively couple plasma spectroscopy and the precipitated sphere-shaped elemental selenium was observed by transmission electron microscopy. CONCLUSION: The bacterial strain SE4-1 isolated in this study suggests a potential role in biogeochemical cycle of selenium by the selenite reduction in the stream environment, and potentials for biotechnological applications to reduceselenium concentrations in selenium-contaminated systems such as wastewater, soil, and groundwater.
Keywords
Bioavailability; Microbial reduction; Selenium; Soil; Toxicity;
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1 Buchs, B., Evangelou, M. W. H., Winkel, L. H. E., & Lenz, M. (2013). Colloidal properties of nanoparticular biogenic selenium govern environmental fate and bioremediation effectiveness. Environmental Science & Technology, 47(5), 2401.   DOI
2 Burton, G. A., Jr., Giddings, T. H., DeBrine, P., & Fall, R. (1987). High incidence of selenite-resistant bacteria from a site polluted with selenium. Applied and Environmental Microbiology, 53(1), 185.
3 Dowdle, P. R., & Oremland, R. S. (1998). Microbial oxidation of elemental selenium in soil slurries and bacterial cultures. Environmental Science and Technology, 32(23), 3749-3755.   DOI
4 Knight, V., Nijenhuis, I., Kerkhof, L., & Haeggblom, M. (2002). Degradation of aromatic compounds coupled to selenate reduction. Geomicrobiology Journal, 19(1), 77-86.   DOI
5 Lee, J.-H., Han, J., Choi, H., & Hur, H.-G. (2007). Effects of temperature and dissolved oxygen on Se (IV) removal and Se (0) precipitation by Shewanella sp. HN-41. Chemosphere, 68(10), 1898-1905.   DOI
6 Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S., Jobb, G., Forster, W., Brettske, I., Gerber, S., Ginhart, A. W., Gross, O., Grumann, S., Hermann, S., Jost, R., Konig, A., Liss, T., Lussmann, R., May, M., Nonhoff, B., Reichel, B., Strehlow, R., Stamatakis, A., Stuckmann, N., Vilbig, A., Lenke, M., Ludwig, T., Bode, A., & Schleifer, K. H. (2004). ARB: a software environment for sequence data. Nucleic Acids Research, 32(4), 1363-1371.   DOI
7 Nuttall, K. L. (2006). Evaluating selenium poisoning. Annals of Clinical and Laboratory Science, 36(4), 409.
8 Oremland, R. S., Blum, J. S., Culbertson, C. W., Visscher, P. T., Miller, L. G., Dowdle, P., & Strohmaier, F. E. (1994). Isolation, growth, and metabolism of an obligately anaerobic, selenate-respiring bacterium, strain SES-3. Applied and Environmental Microbiology, 60(8), 3011.
9 Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., Peplies, J., & Glockner, F. O. (2013). The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res, 41 (Database issue), D590-6.
10 Theisen, J., & Yee, N. (2014). The molecular basis for selenate reduction in Citrobacter freundii. Geomicrobiology Journal, 31(10), 875-883.   DOI