• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.60 no.1
• /
• pp.97-106
• /
• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.42 no.12
• /
• pp.31-36
• /
• 2005

In this paper, we propose a new optical switching system as a infrastructure of the BcN, in which the high traffic volume will be expected due to the multimedia service, like P2P services. Because the JET protocol, the most popular protocol in OBS (Optical Burst Switching) research area, has high blocking probability for burst, it prevents commercialization in real network for its low throughput in TCP layer. To improve high blocking rate in OBS network, we segment large network into small network and perform burst scheduling to avoid burst loss. By using proposed scheme, Internet provider can reduce network deployment cost in Metro network as well as large mesh core networks

• Journal of Broadcast Engineering
• /
• v.10 no.3
• /
• pp.313-320
• /
• 2005

The increasing popularity of multimedia streaming services introduces new challenges in content distribution. Especially, it is important to provide the QoS guarantees as they are increasingly expected to support the multimedia applications. Multimedia streams typically experience the high start-up delay due to the large protocol overhead, the delay, and the loss properties of the wireless networks. The service providers can improve the performance of multimedia streaming by caching the initial segment (prefix) of the popular streams at proxies near the requesting clients. The proxy can initiate transmission to the client while requesting the remainder of the stream from the server. In this paper, we propose the traffic based caching algorithm (TSLRU) to improve the performance of caching proxy. TSLRU classifies the traffic into three types, and improve the performance of caching proxy by reflecting the several elements such as traffic types, recency, frequency, object size when performing the replacement decision. In simulation, TSLRU performs better than the existing schemes in terms of byte hit rate, hit rate, startup latency, and throughput.

• Journal of the Korea Computer Industry Society
• /
• v.3 no.1
• /
• pp.85-94
• /
• 2002

EMRCA(Explicit Max_min Rate Control Algorithm) switch, which has been proposed in the ATM(Asychronous Transfer Mode) standard, controls the ABR(Available Bit Rate) service traffic in the ATM networks. The ABR service class of ATM networks uses a feedback control mechanism to adapt to varying link capacities. The VS/VD(Virtual Source/Virtual Destination) technique offers the possibility to segment the otherwise end-to-end ABR control loop into separate loops. The improved feedback delay and the control of ABR traffic inside closed segments provide a better performance and QoS(Quality of Service) for ABR connections with respect to throughput, delay, and jitter. This paper is study of an ABR VS/VD flow control method. Linear control theory offers the means to derive correct choices of parameters and to assess performance issues, like stability of the system, during the design phase. The performance goals are a high link utilization, fair bandwidth distribution and robust operation in various environments, which are verified by discrete event simulations. The major contribution of this work is the use of linear control theory to model and design an ABR flow control method tailored for the special layout of a VS/VD switch, the simulation shows that this techniques better than conventional method.

• Animal Bioscience
• /
• v.34 no.12
• /
• pp.1921-1929
• /
• 2021

Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai-Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep. Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing. Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep. Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep.

• The KIPS Transactions:PartC
• /
• v.12C no.1 s.97
• /
• pp.121-128
• /
• 2005

Tho existing TCP(Transmission Control Protocol) is known to be unsuitable for a network with the characteristics of high RDP(Bandwidth-Delay Product) because of the fixed small or large buffer size at the TCP sender and receiver. Thus, some trial cases of adjusting the buffer sizes automatically with respect to network condition have been proposed to improve the end-to-end TCP throughput. ATBT(Automatic TCP fluffer Tuning) attempts to assure the buffer size of TCP sender according to its current congestion window size but the ATBT assumes that the buffer size of TCP receiver is maximum value that operating system defines. In DRS(Dynamic Right Sizing), by estimating the TCP arrival data of two times the amount TCP data received previously, the TCP receiver simply reserves the buffer size for the next arrival, accordingly. However, we do not need to reserve exactly two times of buffer size because of the possibility of TCP segment loss. We propose an efficient TCP buffer tuning technique(called TBT-PLR: TCP buffer tuning algorithm based on packet loss ratio) since we adopt the ATBT mechanism and the TBT-PLR mechanism for the TCP sender and the TCP receiver, respectively. For the purpose of testing the actual TCP performance, we implemented our TBT-PLR by modifying the linux kernel version 2.4.18 and evaluated the TCP performance by comparing TBT-PLR with the TCP schemes of the fixed buffer size. As a result, more balanced usage among TCP connections was obtained.

• Journal of Plant Biotechnology
• /
• v.33 no.1
• /
• pp.57-62
• /
• 2006

Several methods for determining the response of corn to glyphosate were investigated to provide a fast and reliable method for identifying glyphosate-resistant corn in vivo. Two bioassays were developed. One assay is named 'whole plant / leaf growth assay', in which the herbicide glyphosate is applied on the upper part of 3rd leaf and the growth of herbicide-untreated 4th leaf is measured at 3 day after treatment. in this assay, the leaf growth of conventional corn was inhibited in a dose dependent from 50 to $1600{\mu}g/mL$ of glyphosate and growth inhibition at $1600{\mu}g/mL$ was 55% of untreated control. The assay has the potential to be used especially in the case that the primary cause of glyphosate resistance is related with a reduction of the herbicide translocation. Another assay is named 'leaf segment / shikimate accumulation assay', in which the four excised leaf segments ($4{\times}4mm$) are placed in each well of a 48-well microtiter plate containing $200{\mu}L$ test solution and the amount of shikimate is determined after incubation for 24 h in continuous light at $25^{\circ}C$. In this assay, 0.33% sucrose added to basic test solution enhanced a shikimate accumulation by 3 to 4 times and the shikimate accumulation was linearly occurred from 2 to $8{\mu}g/mL$ of glyphosate, showing an improved response to the method described by Shaner et al. (2005). The leaf segment / shikimate accumulation assay is simple and robust and has the potential to be used as a high throughput assay in the case that the primary cause of glyphosate resistance is related with EPSPS, target site of the herbicide. Taken together, these two assays would be highly useful to initially select the lines obtained after transformation, to investigate the migration of glyphosate-resistant gene into other weeds and to detect a weedy glyphosate-resistant corn in field.