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Proteome Profiling Unfurl Differential Expressed Proteins from Various Explants in Platycodon Grandiflorum

  • Kim, Hye-Rim (Dept. of Crop Science, Chungbuk National University) ;
  • Kwon, Soo-Jeong (Dept. of Food Nutrition and Cookery, Woosong College) ;
  • Roy, Swapan Kumar (Dept. of Crop Science, Chungbuk National University) ;
  • Cho, Seong-Woo (Rice Research Division, National Institute of Crop Science RDA) ;
  • Kim, Hag-Hyun (Dept. of Food Nutrition and Cookery, Woosong College) ;
  • Cho, Kab-Yeon (Dept. of Food Nutrition and Cookery, Woosong College) ;
  • Boo, Hee-Ock (WellPhyto Co. Ltd., BI Center, GIST) ;
  • Woo, Sun-Hee (Dept. of Crop Science, Chungbuk National University)
  • Received : 2014.12.13
  • Accepted : 2015.02.28
  • Published : 2015.03.31

Abstract

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

Keywords

References

  1. Ahn, K. S., E. J. Noh, H. L. Zhao, S. H. Jung, S. S. Kang, and Y. S. Kim. 2005. Inhibition of inducible nitric oxide synthase and cyclooxygenase II by< i> Platycodon grandiflorum saponins via suppression of nuclear factor-${\kappa}B$ activation in RAW 264.7 cells. Life Sciences. 76 : 2315-2328. https://doi.org/10.1016/j.lfs.2004.10.042
  2. Bandhakavi, S., Y. M. Kim, S. H. Ro, H. Xie, G. Onsongo, C. B. Jun, D. H. Kim, and T. J. Griffin. 2010. Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response. Molecular & Cellular Proteomics. 9 : 403-414. https://doi.org/10.1074/mcp.M900326-MCP200
  3. Cavalier, D. M., O. Lerouxel, L. Neumetzler, K. Yamauchi, A. Reinecke, G. Freshour, O. A. Zabotina, M. G. Hahn, I. Burgert, and M. Pauly. 2008. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component. The Plant Cell Online. 20 : 1519-1537. https://doi.org/10.1105/tpc.108.059873
  4. Choi, J. H., Y. P. Hwang, H. S. Lee, and H. G. Jeong. 2009. Inhibitory effect of Platycodi Radix on ovalbumin-induced airway inflammation in a murine model of asthma. Food and chemical toxicology. 47 : 1272-1279. https://doi.org/10.1016/j.fct.2009.02.022
  5. Choi, Y. H., D. S. Yoo, M. R. Cha, C. W. Choi, Y. S. Kim, S. U. Choi, K. R. Lee, and S. Y. Ryu. 2010. Antiproliferative effects of saponins from the roots of Platycodon grandiflorum on cultured human tumor cells. Journal of natural products. 73 : 1863-1867. https://doi.org/10.1021/np100496p
  6. Decker, G., G. Wanner, M. H. Zenk, and F. Lottspeich. 2000. Characterization of proteins in latex of the opium poppy (Papaver somniferum) using two-dimensional gel electrophoresis and microsequencing. Electrophoresis. 21 : 3500-3516. https://doi.org/10.1002/1522-2683(20001001)21:16<3500::AID-ELPS3500>3.0.CO;2-O
  7. Deshaies, R. J. and C. A. Joazeiro. 2009. RING domain E3 ubiquitin ligases. Annual review of biochemistry. 78 : 399-434. https://doi.org/10.1146/annurev.biochem.78.101807.093809
  8. Faik, A., N. J. Price, N. V. Raikhel, and K. Keegstra. 2002. An Arabidopsis gene encoding an ${\alpha}$-xylosyltransferase involved in xyloglucan biosynthesis. Proceedings of the National Academy of Sciences. 99 : 7797-7802. https://doi.org/10.1073/pnas.102644799
  9. Finnie, C., S. Melchior, P. Roepstorff, and B. Svensson. 2002. Proteome analysis of grain filling and seed maturation in barley. Plant Physiology. 129 : 1308-1319. https://doi.org/10.1104/pp.003681
  10. Foyer, C. H., A. J. Bloom, G. Queval, and G. Noctor. 2009. Photorespiratory metabolism: genes, mutants, energetics, and redox signaling. Annual review of plant biology. 60 : 455-484. https://doi.org/10.1146/annurev.arplant.043008.091948
  11. Goward, C. R. and D. J. Nicholls. 1994. Malate dehydrogenase: a model for structure, evolution, and catalysis. Protein Science. 3 : 1883-1888. https://doi.org/10.1002/pro.5560031027
  12. Han, L. K., B. J. Xu, Y. Kimura, Y. N. Zheng, and H. Okuda. 2000. Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. The Journal of nutrition. 130 : 2760-2764.
  13. Hung, Y. C., P. W. Wang, and T. L. Pan. 2010. Functional proteomics reveal the effect of< i> Salvia miltiorrhiza aqueous extract against vascular atherosclerotic lesions. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics. 1804 : 1310-1321. https://doi.org/10.1016/j.bbapap.2010.02.001
  14. Jacobs, D. I., M. Gaspari, J. van der Greef, R. van der Heijden, and R. Verpoorte. 2005. Proteome analysis of the medicinal plant Catharanthus roseus. Planta. 221 : 690-704. https://doi.org/10.1007/s00425-004-1474-4
  15. Kamal, A. H. M., I. D. Jang, D. E. Kim, T. Suzuki, K. Y. Chung, J. S. Choi, M. S. Lee, C. H. Park, S. U. Park, S. H. Lee, H. S. Jeong, and S. H. Woo. 2011. Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds. J. Plant Biol. 54 : 81-91. https://doi.org/10.1007/s12374-010-9143-6
  16. Kenmochi, N., T. Kawaguchi, S. Rozen, E. Davis, N. Goodman, T. J. Hudson, T. Tanaka, and D. C. Page. 1998. A map of 75 human ribosomal protein genes. Genome research. 8 : 509-523.
  17. Kennedy, J. 2005. Herb and supplement use in the US adult population. Clinical therapeutics. 27 : 1847-1858. https://doi.org/10.1016/j.clinthera.2005.11.004
  18. Khanal, T., J. H. Choi, Y. P. Hwang, Y. C. Chung, and H. G. Jeong. 2009. Protective effects of saponins from the root of< i> Platycodon grandiflorum against fatty liver in chronic ethanol feeding via the activation of AMP-dependent protein kinase. Food and chemical toxicology. 47 : 2749-2754. https://doi.org/10.1016/j.fct.2009.08.006
  19. Kim, J. Y., Y. P. Hwang, D. H. Kim, E. H. Han, Y. C. Chung, S. H. Roh, and H. G. Jeong. 2006. Inhibitory effect of the saponins derived from roots of Platycodon grandiflorum on carrageenan induced inflammation. Bioscience, biotechnology, and biochemistry. 70 : 858-864. https://doi.org/10.1271/bbb.70.858
  20. Kim, K. S., O. Ezaki, S. Ikemoto, and H. Itakura. 1995. Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia. Journal of nutritional science and vitaminology. 41 : 485-491. https://doi.org/10.3177/jnsv.41.485
  21. Kosugi, S. and Y. Ohashi. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. The Plant Journal. 30 : 337-348. https://doi.org/10.1046/j.1365-313X.2002.01294.x
  22. Lee, J. Y., J. W. Yoon, C. T. Kim, and S. T. Lim. 2004. Antioxidant activity of phenylpropanoid esters isolated and identified from< i> Platycodon grandiflorum A. DC. Phytochemistry. 65 : 3033-3039. https://doi.org/10.1016/j.phytochem.2004.08.030
  23. Lin, T. H. and C. L. Hsieh. 2010. Review Pharmacological effects of Salvia miltiorrhiza (Danshen) on cerebral infarction.
  24. Lu, J. Y., Y. Y. Lin, J. Qian, S. C. Tao, J. Zhu, C. Pickart, and H. Zhu. 2008. Functional dissection of a HECT ubiquitin E3 ligase. Molecular & Cellular Proteomics. 7 : 35-45.
  25. Mittler, R. and B. A. Zilinskas. 1994. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. The Plant Journal. 5 : 397-405. https://doi.org/10.1111/j.1365-313X.1994.00397.x
  26. Mukherjee, S. and M. Choudhuri. 1983. Implications of water stressinduced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia plantarum. 58 : 166-170. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x
  27. Nam, M. H., S. I. Kim, J. R. Liu, D. C. Yang, Y. P. Lim, K. H. Kwon, J. S. Yoo, and Y. M. Park. 2005. Proteomic analysis of Korean ginseng ( Panax ginseng CA Meyer). Journal of Chromatography B. 815 : 147-155. https://doi.org/10.1016/j.jchromb.2004.10.063
  28. Nawrot, R., A. Kalinowski, and A. Gozdzicka-Jozefiak. 2007. Proteomic analysis of Chelidonium majus milky sap using twodimensional gel electrophoresis and tandem mass spectrometry. Phytochemistry. 68 : 1612-1622. https://doi.org/10.1016/j.phytochem.2007.03.039
  29. Ning, C. J., N. Ding, G. L. Wu, H. J. Meng, Y. N. Wang, and Q. H. Wang. 2013. Proteomics research on the effects of applying selenium to apple leaves on photosynthesis. Plant Physiology and Biochemistry. 70 : 1-6. https://doi.org/10.1016/j.plaphy.2013.05.008
  30. Noctor, G., S. Veljovic-Jovanovic, S. Driscoll, L. Novitskaya, and C. H. Foyer. 2002. Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration? Annals of Botany. 89 : 841-850. https://doi.org/10.1093/aob/mcf096
  31. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 250 : 4007-4021.
  32. Oh, Y. C., O. H. Kang, J. G. Choi, Y. S. Lee, O. O. Brice, H. J. Jung, S. H. Hong, Y. M. Lee, D. W. Shin, and Y. S. Kim. 2010. Anti-allergic activity of a platycodon root ethanol extract. International Journal of Molecular Sciences. 11 : 2746-2758. https://doi.org/10.3390/ijms11072746
  33. Park, Y. S., Y. Yoon, and H. S. Ahn. 2007. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World Journal of Gastroenterology. 13 : 3493. https://doi.org/10.3748/wjg.v13.i25.3493
  34. Patwardhan, B., D. Warude, P. Pushpangadan, and N. Bhatt. 2005. Ayurveda and traditional Chinese medicine: a comparative overview. Evidence-Based Complementary and Alternative Medicine. 2 : 465-473. https://doi.org/10.1093/ecam/neh140
  35. Rehm, S. and G. Espig. 1991. The cultivated plants of the tropics and subtropics, CTA.
  36. Richau, K. H., F. Kaschani, M. Verdoes, T. C. Pansuriya, S. Niessen, K. Stuber, T. Colby, H. S. Overkleeft, M. Bogyo, and R. A. Van der Hoorn. 2012. Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics. Plant Physiology. 158 : 1583-1599. https://doi.org/10.1104/pp.112.194001
  37. Rizhsky, L., H. Liang, and R. Mittler. 2002. The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiology. 130 : 1143-1151. https://doi.org/10.1104/pp.006858
  38. Sachs, A. B., R. Davis, and R. Kornberg. 1987. A single domain of yeast poly (A)-binding protein is necessary and sufficient for RNA binding and cell viability. Molecular and Cellular Biology. 7 : 3268-3276. https://doi.org/10.1128/MCB.7.9.3268
  39. Sawicki, G. and B. I. Jugdutt. 2007. Valsartan reverses posttranslational modifications of the ${\delta}$-subunit of ATP synthase during in vivo canine reperfused myocardial infarction. Proteomics. 7 : 2100-2110. https://doi.org/10.1002/pmic.200601022
  40. Tada, A., Y. Kaneiwa, J. Shoji, and S. Shibata. 1975. Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D. Chemical & pharmaceutical bulletin. 23 : 2965. https://doi.org/10.1248/cpb.23.2965
  41. Wang, C., G. B. Schuller Levis, E. B. Lee, W. R. Levis, D. W. Lee, B. S. Kim, S. Y. Park, and E. Park. 2004. Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-${\alpha}$ in activated RAW 264.7 cells. International immunopharmacology. 4 : 1039-1049. https://doi.org/10.1016/j.intimp.2004.04.005
  42. Williamson, E. M. 2003. Drug interactions between herbal and prescription medicines. Drug Safety. 26 : 1075-1092. https://doi.org/10.2165/00002018-200326150-00002
  43. Zhao, H., J. Sim, S. Shim, Y. Ha, S. Kang, and Y. Kim. 2005. Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction. International Journal of Obesity. 29 : 983-990. https://doi.org/10.1038/sj.ijo.0802948
  44. Zhao, H. L., K. H. Cho, Y. W. Ha, T. S. Jeong, W. S. Lee, and Y. S. Kim. 2006. Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice. European journal of pharmacology. 537 : 166-173. https://doi.org/10.1016/j.ejphar.2006.03.032

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