• Title/Summary/Keyword: seedling soil

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Effects of ectomycorrhizal fungi on soil-borne plant pathogenic fungi in red pine seedlings

  • Seo, Il-Won;Lee, Jong-Kyu
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.89.1-89
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    • 2003
  • Disease suppression by ectomycorrhizal(ECM) fungi has been demonstrated on red pine seedlings. Culturing of pathogenic fungi on petri plates containing culture filtrates of ECM fungi showed that culture filtrates of the ECM fungus Hebeloma cylindrosporum may inhibit the mycelial growth of all tested soil-borne plant pathogenic(SBPP) fungi upto 60%, In order to examine the effects of ECM fungi on SBPP fungi and on red pine seedlings, both symbiotic and pathogenic fungi were inoculated into the soil with red pine seedlings by three inoculation methods; pre-inoculation of SBPP fungi 10 days before inoculation of ECM fungi, simultaneous inoculation of both fungi, post-inoculation of SBPP fungi 60 days after inoculation of ECM fungi. Seedling mortality, seedling growth, and ectomycorrhizal formation by the combined treatments were examined and compared. Pine seedlings were dead by the pre-inoculation of pathogenic fungi, except Rhizina undulate which required 9-12 days, within 6 days after inoculation. Among pathogenic fungi tested, Fusarium oxysporum was the most pathogenic with the mortality of 44%. However, no dead seedlings were shown by simultaneous inoculation of both fungi or pre-inoculation of ECM fungi. In addition, pine seedlings treated by simultaneous or post-inoculation of SBPP fungi were relatively higher than those treated by pre-inoculation in diameter at root crown and the number of ectomycorrhizal roots. There were no significant differences among inoculation methods in root length and dry weight of treated seedlings. It means that ECM fungi somehow play a role in protecting primary roots of red pine seedlings against invasion by the SBPP fungi.

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Development of a Hopper-Type Planting Device for a Walk-Behind Hand-Tractor-Powered Vegetable Transplanter

  • Dihingia, Pramod Chandra;Prasanna Kumar, G.V.;Sarma, Pallab Kumar
    • Journal of Biosystems Engineering
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    • v.41 no.1
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    • pp.21-33
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    • 2016
  • Purpose: In order to ensure that vegetable seedlings (with a soil block around their roots) are planted in an upright orientation after metering in a vegetable transplanter, they need to be dropped freely from a certain height. The walk-behind hand-tractor-powered machines do not have sufficient space to drop the seedlings from that height. In the present work, a hopper-type planting device was developed for the walk-behind hand-tractor-powered vegetable transplanter to ensure that the soil block seedlings are planted in an upright orientation. Methods: Various dimensionless terms were developed based on the dimensional analysis approach, and their effect on the planting of soil block seedlings in an upright orientation (planting efficiency) was studied. The optimum design dimensions of the hopper-type planting device were identified by the Taguchi method of optimization. Results: The ratio of the height of free fall to the sliding distance of the seedling on the surface of the hopper had the highest influence on planting efficiency. The planting efficiency was highest for plants with a height $15{\pm}2cm$. The plant handling Froude number, in interaction with the design of the hopper-type planting device, also significantly affected the planting efficiency. Of the hopper design factors, the length of the slide of the seedlings on the surface of the hopper was most important, and induced sufficient velocity and rotation to cause the seedling to fall in an upright orientation. An evaluation of the performance of the planting device under actual field conditions revealed that the planting efficiency of the developed planting device was more than 97.5%. Conclusions: As the seedlings were fed to the metering device manually, an increase in planting rate increased missed plantings. The planting device can be adopted for any vegetable transplanter in which the seedlings are allowed to drop freely from the metering device.

Seedling Growth of Red Pepper and Antagonism on Alternaria alternata Causing Black Rot by Bacillus subtilis J-24 isolated from Red Pepper Rhizosphere (고추 근권에서 분리한 Bacillus subtilis J-24의 검은 곰팡이병원균 Alternaria alternata에 대한 길항력 및 고추의 초기 생육에 미치는 영향)

  • Joo, Gil-Jae;Kim, Hak-Yoon;Hur, Sang-Sun;Woo, Churl-Joo;Rhee, In-Koo
    • Current Research on Agriculture and Life Sciences
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    • v.17
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    • pp.7-13
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    • 1999
  • Thirty bacteria were isolated from the red pepper rhizosphere. The isolates were screened for antagonism to Alternaria alteranta causing red pepper black rot. Antagonistic bacterium No. J-24 was selected among the isolated bacteria and was identified as Bacillus subtilis based on morphological and physiological characteristics and MIDI system. B. subtilis J-24 showed antifungal activities against A. alternata(inhibition percentage, 99%), Botrytis cinerea, Phytophthora capsici, Pythium ultimum, Colletotrichum gloeosporioides, Stemphylium botryosum. The growth of red pepper seedling was promoted as compared to control when the microbial inoculants was mixed in bed soil. In the mixed microbial inoculants bed soil, the leaf area of red pepper was increased of 15 percent, the hypocotyl weight 12 percent, the root length 12 percent, total dry weight 13 percent as compared to those grown in the general bed soil.

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Density and Species Composition of Soil Seed Bank in Rural Stream Topsoil (농촌하천 표토내 매토종자의 발아량 및 종구성)

  • Kim, Se-Chang;Park, Bong-Ju;Kim, Won-Tae;Yoon, Yong-Han;Cho, Yong-Hyeon;Kang, Hee-Kyoung;Oh, Hyun-Kyung;Shin, Kyung-Jun;Eo, Yang-Joon;Yoon, Taek-Seong;Jang, Kwang-Eun;Kwak, Moo-Young
    • Journal of Environmental Science International
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    • v.21 no.11
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    • pp.1419-1424
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    • 2012
  • Purpose of this study was to evaluate germination characteristics of soil seed bank in rural stream topsoil using seedling emergence method in order to provide data for future ecological restoration of stream utilizing topsoil. There were 24 families, 52 genera, 61 taxa of soil seed bank flora found in topsoil from 6 rural streams. The most frequently found taxa were Compositae (12 taxa) followed by Gramineae (8 taxa), Caryophyllaceae (5 taxa), Cruciferae (4 taxa), Scrophulariaceae, Labiatae, Polygonaceae and Cyperaceae. Plant with the most number of germination was Stellaria aquatica followed by Erigeron annuus, Imperata cylindrica var. koenigii, Poa annua, Cyperus microiria and Veronica undulata. Naturalized plants found were Erigeron annuus, Rumex crispus, Oenothera odorata, Cerastium glomeratum, Bidens frondosa, Erigeron philadelphicus, etc.

Selection of Suitable Organic Matter for To-jik Nursery in Panax ginseng C.A. Meyer (인삼 재배 시 토직모 생산에 적합한 유기물 선발)

  • Kim, Dong-Won;Kim, Hee-Jun;Park, Jong-Suk;Kim, Dae-Hyang;Cheong, Seong-Soo;Ryu, Jeong
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.2
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    • pp.74-78
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    • 2010
  • This experiment was carried out to select suitable organic matter in To-jik nursery (self soil nursery) for complement To-jik nursery's defects that are deterioration of raw material by poor quality of seed ginseng and reduction of the quantity in seed ginseng production. Organic matter used were Yacto, rice bean, defatted rice bran, soybean cake and their mixture. As follows, bulk density in soil physical property by treating organic composts was the greatest in soybean cake and the next was followed by mix, Yacto, defatted rice bran, and rice bran treatment in order. Soil pore space ratio was totally the opposite; that was rice bran the first and followed by defatted rice bran, Yacto, mix and soybean cake treatment. The incidence rate of damping off by treating organic composts was 1.5% in both soybean cake and mix while the others was 1.0%. Emergence time was the same among treatment on April 16 and Emergence rate was the highest at 73% in Yacto. There was no significant differences among treatment in the growth of aboveground part but it was a little better in defatted rice bran treatment. In Yacto treatment, the growth of underground part, total root number per kan, rate of first grade ginseng seedling, and rate of usable ginseng seedling etc. were entirely higher but there was little differences. Using defatted rice bran was slightly lower in productivity compared to Yacto, but the possibility was high as a alternative for Yacto in a view of managing cost down.

Investigation of Emergence Conditions and Plug Seedling Periods in Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (지황의 출아조건 및 플러그 육묘기간 구명 연구)

  • Lee, Sang Hoon;Koo, Sung Cheol;Hur, Mok;Lee, Woo Moon;Park, Min Su;Han, Jong Won
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.4
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    • pp.271-277
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    • 2019
  • Background: Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. has long been used as a traditional medicinal plant in Korea. This study was carried out to investigate the emergence conditions during the seedling periods in R. glutinosa. Methods and Results: The rhizomes of R. glutinosa variety (Jihwang 1) were harvested in the 22, March, 2018. The rhizomes were sown on in 50-cell plug trays. The emergence rates of seedlings at $15^{\circ}C$, $20^{\circ}C$, $25-40^{\circ}C$, and $45^{\circ}C$ treatment seedling were 1.3%, 96%, 100% and 0%, respectively. Rhizome rot was occurred at the temperature of $15^{\circ}C$ and $45^{\circ}C$. The emergence rates of seedlings in high moisture (HM), moderate moisture (MM) and low moisture (LM) treatments at $35^{\circ}C$ were 99.3%, 100%, and 0%, respectively. Drought damage was recorded in plant with the LM treatment. Seedling quality surveys were carried out at 10-days intervals from 10 to 60 days after sowing (DAS). Leaf length and leaf width were increasing until 50 DAS and the number of leaves was increasing until 60 DAS. Root length was increasing until 40 DAS, and then, flowering occurred from 30 to 60 DAS. Lastly, at 40 DAS, leaf aging and root enlargement was observed. Conclusions: We concluded that the emergence of seedlings was possible in the range of 20 to $40^{\circ}C$. Considering drying and rotting damage, we concluded that the moderate level of moisture is most appropriate for seedling emergence. In addition, we concluded that optimal seedling periods are between 30 and 40 DAS.

The Effects of Fertilization on Growth Performances and Physiological Characteristics of Liriodendron tulipifera in a Container Nursery System (시비 처리가 백합나무 용기묘의 생장 및 생리적 특성에 미치는 영향)

  • Cho, Min Seok;Lee, Soo Won;Park, Byung Bae;Park, Gwan Su
    • Journal of Korean Society of Forest Science
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    • v.100 no.2
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    • pp.305-313
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    • 2011
  • Fertilization is essential to seedling production in nursery culture, but excessive fertilization can contaminate surface and ground water around the nursery. The objective of this study was to find optimal fertilization practice of container seedling production for reducing soil and water contamination around the nursery without compromising seedling quality. This study was conducted to investigate growth performance, photosynthesis, chlorophyll fluorescence, and chlorophyll contents of Liriodendron tulipifera growing under three different fertilization treatments (Constant rate, Three-stage rate, and Exponential rate fertilization). Root collar diameter, height, and biomass of L. tulipifera were the highest at Constant treatment. Like growth performance, seedling quality index (SQI) were higher at Constant than at other treatments, but not significantly different among treatments. L. tulipifera showed good photosynthetic capacity at all treatments. Photochemical efficiency and chlorophyll contents were significantly lower at Exponential than at other treatments. Therefore, Exponential fertilization which is 50% fertilizer of other treatments would maximize seedling growth and minimize nutrient loss.

The Effects of Water Holding Matter on Emergence and Early Growth of Forage Grasses (보수성물질이 목초의 출아 및 초기생육에 미치는 영향)

  • 윤세형
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.3
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    • pp.153-157
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    • 1991
  • The present study eluciciated the effect of water holding matter on emergence and survival of forage grasses in seedling and early growth stage. Vermiculite was used as u7ater holding matter. Experiment was conducted with 2 treatments, soil only and soil with 3% vermiculite. The results obtained were as follows: Most of character related to emergence and early growth were improved clearly by adding vermiculite. Specially, root growth was increased when vermiculite was added in soil. Survival percent at drought stress condition was also improved by adding vermiculite. Rut the amount of water keeping by verm~culite was not much.

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The effect of soil heterogeneity and container length on the growth of Populus euramericana in a greenhouse study

  • Rahman, Afroja;Meng, Loth;Han, Si Ho;Seo, Gi Chun;Jung, Mun Ho;Park, Byung Bae
    • Korean Journal of Agricultural Science
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    • v.45 no.2
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    • pp.143-153
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    • 2018
  • Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

Ecological Studies on Lettuce Drop Disease Occurring under Controlled Cultivation Conditions in Drained Paddy Fields (답리작 상치 시설재배지에서의 균핵병 발생생태에 관한 연구)

  • Shin Dong Bum;Lee Joon Tak
    • Korean Journal Plant Pathology
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    • v.3 no.4
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    • pp.252-260
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    • 1987
  • Incidence of lettuce drop was observed throughout the growing season in the vinylhouse at the southern part of Korea, Kimhai. Occurrence of this disease was especially severe at the seedling stage. Number of sclerotia in surface soil $(30\times30\times5cm)$ was 22.0 at the seedling stage, and 5.3 at harvest in the infected area. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature at $25^{\circ}C$. Sclerotia were formed fewer at low temperature, but their size was larger resulting in heavier dry weight than that at high temperature. The apothecia were formed from the sclerotia that were buried in March, April and September upto 3cm soil depth, but formed from those buried only 1 em soil depth in October. Sclerotia buried in June and December did not form apothecia regardless of soil depth by 90 days. The sclerotia buried in the 5cm of soil depth did not form apothecia. Sclerotia that were embedded in wet or flooded soil at $25^{\circ}C$ and $30^{\circ}C$ for 5 weeks lost their viability. Infection of lettuce was possible with mycelia originated from sclerotia on autoclaved lettuce plant fragments. The fungus was pathogenic on 25 plant species in 8 families in artificial inoculation tests. Lettuce seedlings appeared to be infected by airborne ascospore originated from sclerotia on crops and weeds around paddy fields, because sclerotia existing in soil might perish under long flood conditions during rice cultivation.

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