• 한국초지조사료학회지
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• 제21권3호
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• pp.157-162
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• 2001

"Hwasan 102" is a new cold-tolerant Italian ryegrass(Lo1ium rnultiflorum L.) variety developed by theNational Livestock Research Institute(NLRI) in 1999. Having cold tolerance in Italian ryegrass is an importantfor enlargement of cultivation area and increase of productivity in Korea. To develop the cold-tolerant varietyof Italian ryegrass, cold-tolerant clones survived under - 13- - 14$^{\circ}$C of minimum average air temperature(MAAT) in January were selected at Dun Nae, Kwangwon Province in 1993. Five of selected clones werepolycrossed for seed production by NLRI, RDA, in 1995."Hwasan 102" was tetraploid variety, dark green in leaf color and broad in flag leaf width. Also it hassemi-prostrate and medium growth habit in late autumn and in early spring, respectively. "Hwasan 102" withlow plant height at harvesting time was excellent in lodging tolerance. First heading date of "Hwasan 102"was 19th May, it was similar to that of Marshall. Expecially, "Hwasan 102" survived about 60% under - 10- - 12$^{\circ}$C of MAAT in January, so it could be cold-tolerant variety that can be safely cultivated in regionshigher than -9'C of MAAT in January. Compared with Marshall, "Hwasan 102" showed 8% higher freshyield (59.2MT per ha) but it showed 2% lower dry matter yield (9.6MT per ha). Higher IVDMD and TDNand lower ADF and NDF were observed in "Hwasan 102" than those of Florida 80 and Marshall in Foragequality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 102")rshall in Foragequality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 102")san 102&")ot;)

• 한국작물학회지
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• 제61권2호
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• pp.138-144
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• 2016

The present study was performed to compare the morpho-physiological characteristics of the tetraploid and diploid varieties of Platycodon grandiflorum and to obtain basic data for cultivating a tetraploid variety with high yield and content of functional substances. The plant height of the tetraploid variety (54.0 cm) was slightly higher than that of the diploid variety. The leaf length and width of the tetraploid variety were 10.2 cm and 7.3 cm, respectively. The results obtained from the present study revealed that the form of the leaf changed from lanceolate to ovate, and the chlorophyll content in the tetraploid variety (16.7) was slightly higher than that in the diploid variety. The photosynthetic rate significantly increased (24%) to $13.4{\mu}mol\;CO_2{\cdot}m^{-2}{\cdot}s^{-1}$ in the tetraploid variety from that of the diploid variety. The pollen viability of the tetraploid variety was decreased by approximately 33% with respect to that of the diploid variety, but this did not have a significant adverse effect on seed production. The fresh weight of tetraploid P. grandiflorum was 49.4 g, which was approximately 44% higher than that of the diploid variety.

• 한국약용작물학회지
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• 제4권1호
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• pp.38-46
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• 1996

길경의 화색에 따른 생육특성과 수량 및 품질에 차이가 나타나는지 그리고 재배년차에 따른 길경의 뿌리생육과 수량 및 사포닌과 정유함량 등에 차이가 있는지를 알아보고자 실험한 결과를 요약하면 다음과 같다. 1. 백화길경이 자화길경에 비해 줄기수, 꼬투리수, 초장, 종자수량과 같은 지상부 생육과 생근중, 건근중, 근장 및 근직경 등과 같은 뿌리 형질에서 다소 높은 경향을 보였으나 유의차는 없었다. 또한 사포닌 함량이나 정유 함량에도 차이가 없었다. 2. 2년생과 3년생에서 초장은 차이가 없었으나 개체당 줄기수와 꼬투리수는 3년생에서 많았다. 생근중, 근장 및 근직경은 3년생에서 높았다. 사포닌 함량과 정유함량은 2년생과 3년생간에 큰 차이를 보이지 않았다. 사포닌 함량은 2년생, 3년생 모두에서 5월에 채취한 것이 가장 높았고 9월에 채취한 것이 가장 낮았다. 정유함량은 8월에 채취한 것이 가장 높은 함량을 보였고 4월에 채취한 것이 가장 낮았다.

• 화훼연구
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• 제19권1호
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• pp.1-7
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• 2011

본 연구는 나리 하계 고온기 고품질 절화생산 및 경영비 절감을 위한 도입 소구의 비대증식에 유리한 환경조건을 찾아내기 위해 수행하였다. 종구비 절감을 위해 소구를 도입하여 양구하는데 효과적인 환경조건을 찾아내고자 재배형태를 비가림, 망실, 무피복으로 나누고 다시 절화 재배시 많이 사용하는 30% 차광처리와 무처리로 구분하여 시험하였다. 모든 처리구에서 비가림 재배가 우수하였고 차광의 효과도 다소 있었다. 특히 양구에 있어서는 대관령 등의 고랭지에서 주간과 야간온도의 차이가 커서 동화산물의 전류 및 축적이 많아 평지에 비해 구비대가 유리하여 시비 및 관수방법에 따라 보통 3년 정도인 양구 기간을 단축할 수도 있을 것으로 기대된다.

• 한국육종학회지
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• 제43권3호
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• pp.196-200
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• 2011

'슈퍼자미'는 한국방송통신대학교 농학과에서 2009년도에 육성한 C3G 고함유 흑자색 품종으로 주요특성과 수량성을 요약하면 다음과 같다. 1. 중부평야지 평균 출수기는 8월 25일로 만생종이며, 간장은 75 cm 정도이고, 종피색이 흑자색인 유색메벼 품종이다. 2. 현미천립중은 26.2 g 정도이고, 장폭비는 1.87로 단원형이며, 종피는 흑자색으로 안토시아닌 주색소인 C3G 함량이 '흑진주벼'의 10배 수준으로 색택이 균일한 품종이다. 3. 중부평야지역 보통기 보비재배 2개소에서 평균 629.7 kg/10a 수준으로 '흑진주벼'의 162% 수준이다.

• Journal of Ginseng Research
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• 제43권3호
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• pp.482-487
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• 2019

Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects on stability of yield and quality. K-1 is a superior cultivar with good root shape and stronger disease resistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associate with major traits and can be used for marker-assisted selection to maintain the high quality of Korean ginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified and compared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein were analyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotide polymorphism (SNP)-based specific primer was designed for K-1 by introducing a destabilizing mismatch within the 3' end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specific PCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparagine was exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of amino acid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designed for specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-time allele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large number of ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not only for marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs of P. ginseng.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.247-250
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• 2019

Wheat is one of the most important grains. Its consumption is increasing globally. Many countries are making efforts to increase the extent of wheat harvest. It is known that plant growth promoting rhizobacteria (PGPRs) have beneficial effects on various plants. Two PGPRs including Paenibacillus pabuli strain P7S (PP7S) and Pseudomonas nitroreducens strain IHB (PnIHB) were employed to investigate effects of PGPRs on early growth of three wheat cultivars (Koso, Seakumkang, and Jokyung). While PP7S had adverse effects on Seakumkang and Jokyung, PP7S had positive effects on Koso except root length compared to control group having no treatment of PP7S. However, all treatments with PnIHB had adverse effects on germination rate, root/shoot lengths, vigor index, and dry root/shoot weights of all three wheat cultivars. These positive effects with PP7S on Koso might be related to the earlier emergence of wheat seed above soil which is known to be an indicator of increased yield. Results of the present study suggest that if proper PGPR strains are selected, they could have positive effects on early growth rate of a wheat cultivar.

• The Plant Pathology Journal
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• 제37권2호
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• pp.194-199
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• 2021

Blackleg is a serious disease in Brassica plants, causing moderate to severe yield losses in rapeseed worldwide. Although China has not suffered from this disease yet (more aggressive Leptosphaeria maculans is not present yet), it is crucial to take provisions in breeding for disease resistance to have excellent blackleg-resistant cultivars already in the fields or in the breeding pipeline. The most efficient strategy for controlling this disease is breeding plants with identified resistance genes. We selected 135 rapeseed accessions in Sichuan, including 30 parental materials and 105 hybrids, and we determined their glucosinolate and erucic acid content and confirmed 17 double-low materials. A recently developed single-nucleotide polymorphism (SNP) marker, SNP_208, was used to genotype allelic Rlm1/rlm1 on chromosome A07, and 87 AvrLm1-resistant materials. Combined with the above-mentioned seed quality data, we identified 11 AvrLm1-resistant double-low rapeseed accessions, including nine parental materials and two hybrids. This study lays the foundation of specific R gene-oriented breeding, in the case that the aggressive Leptosphaeria maculans invades and establishes in China in the future and a robust and less labor consuming method to identify resistance in canola germplasm.

• Genomics & Informatics
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• 제19권2호
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• pp.19.1-19.9
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• 2021

Plant height is an important component of plant architecture and significantly affects crop breeding practices and yield. We studied DNA variations derived from F5 recombinant inbred lines (RILs) with 96.8% homozygous genotypes. Here, we report DNA variations between the normal and dwarf members of four lines harvested from a single seed parent in an F6 RIL population derived from a cross between Glycine max var. Peking and Glycine soja IT182936. Whole genome sequencing was carried out, and the DNA variations in the whole genome were compared between the normal and dwarf samples. We found a large number of DNA variations in both the dwarf and semi-dwarf lines, with one single nucleotide polymorphism (SNP) per at least 3.68 kb in the dwarf lines and 1 SNP per 11.13 kb of the whole genome. This value is 2.18 times higher than the expected DNA variation in the F6 population. A total of 186 SNPs and 241 SNPs were discovered in the coding regions of the dwarf lines 1282 and 1303, respectively, and we discovered 33 homogeneous nonsynonymous SNPs that occurred at the same loci in each set of dwarf and normal soybean. Of them, five SNPs were in the same positions between lines 1282 and 1303. Our results provide important information for improving our understanding of the genetics of soybean plant height and crop breeding. These polymorphisms could be useful genetic resources for plant breeders, geneticists, and biologists for future molecular biology and breeding projects.

• 한국작물학회지
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• 제28권2호
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• pp.233-239
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• 1983

종전에는 Brassica napus에서 성분이 개량되지 않은 유채의 일반 품종들의 Winter type만을 대상으로 춘파성 정도를 분류하고 추태로서 분류기준을 삼았으나 본 시험에서는 같은 B. napus인 Summer type까지를 포함하고 성분이 개량된 양질유ㆍ양박 품종을 공시하여 임실여부로서 분류기준을 삼는 새로운 분류를 시도하여 춘파성 정도를 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. 현재 목포지장에서 보유하고 있는 양질유ㆍ양박 유채(Brassica napus) 품종 및 육성계통들은 8단계 group으로 춘파성 정도를 분류할 수 있었고 춘파성 정도 제Ⅶ group는 Summer type의 계통들이 이에 속하였으며 이들 계통은 Oro, Midas 등의 Summer type의 품종과 교배하여 선발된 계통들이었다. 2. 춘파 파종기에 따른 성숙기변화는 춘파시기가 늦을수록 성숙기는 지연되는 경향이었고 춘파성 정도가 높음에 따라 생육일수와 적산온도가 줄어드는 경향이었다. 3. 춘파성 정도와 내한성 관계는 춘파성 정도가 높을수록 내한성이 낮았으며 춘파성 정도와 초성간에는 춘파성이 높을수록 초성에서 I형에 가깝고 주경의 신장이 분지보다 빈약한 바 이들 모든 상호관계는 고도의 부상관으로 나타났다. 4. 월동에 안전한 추파재배용계통은 춘파성 정도가 낮은 0, I, II group로서 공시품종중 59 품종이 이에 속하였다.