Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR |
Chai, Liang
(Crop Research Institute, Sichuan Academy of Agricultural Sciences)
Zhang, Jinfang (Crop Research Institute, Sichuan Academy of Agricultural Sciences) Dilantha Fernando, Wannakuwattewaduge Gerard (Department of Plant Science, University of Manitoba) Li, Haojie (Crop Research Institute, Sichuan Academy of Agricultural Sciences) Huang, Xiaoqin (Institute of Plant Protection, Sichuan Academy of Agricultural Sciences) Cui, Cheng (Crop Research Institute, Sichuan Academy of Agricultural Sciences) Jiang, Jun (Crop Research Institute, Sichuan Academy of Agricultural Sciences) Zheng, Benchuan (Crop Research Institute, Sichuan Academy of Agricultural Sciences) Liu, Yong (Institute of Plant Protection, Sichuan Academy of Agricultural Sciences) Jiang, Liangcai (Crop Research Institute, Sichuan Academy of Agricultural Sciences) |
1 | Marcroft, S. J., Van de Wouw, A. P., Salisbury, P. A., Potter, T. D. and Howlett, B. J. 2012. Effect of rotation of canola (Brassica napus) cultivars with different complements of blackleg resistance genes on disease severity. Plant Pathol. 61:934-944. DOI |
2 | Semagn, K., Babu, R., Hearne, S. and Olsen, M. 2014. Single nucleotide polymorphism genotyping using kompetitive allele specific PCR (KASP): overview of the technology and its application in crop improvement. Mol. Breed. 33:1-14. DOI |
3 | Sun, L., Wang, X., Yu, K., Li, W., Peng, Q., Chen, F., Zhang, W., Fu, S., Xiong, D., Chu, P., Guan, R. and Zhang, J. 2018. Mapping of QTLs controlling seed weight and seed-shape traits in Brassica napus L. using a high-density SNP map. Euphytica 214:228. DOI |
4 | Van de Wouw, A. P., Cozijnsen, A. J., Hane, J. K., Brunner, P. C., McDonald, B. A., Oliver, R. P. and Howlett, B. J. 2010. Evolution of linked avirulence effectors in Leptosphaeria maculans is affected by genomic environment and exposure to resistance genes in host plants. PLoS Pathog. 6:e1001180. DOI |
5 | Larkan, N. J., Ma, L., Haddadi, P., Buchwaldt, M., Parkin, I. A. P., Djavaheri, M. and Borhan, M. H. 2020. The Brassica napus wall-associated kinase-like (WAKL) gene Rlm9 provides race-specific blackleg resistance. Plant J. 104:892-900. DOI |
6 | Delourme, R., Chevre, A. M., Brun, H., Rouxel, T., Balesdent, M. H., Dias, J. S., Salisbury, P., Renard, M. and Rimmer, S. R. 2006. Major gene and polygenic resistance to Leptosphaeria maculans in oilseed rape (Brassica napus). Eur. J. Plant Pathol. 114:41-52. DOI |
7 | Liu, Z., Latunde-Dada, A. O., Hall, A. M. and Fitt, B. D. L. 2014. Phoma stem canker disease on oilseed rape (Brassica napus) in China is caused by Leptosphaeria biglobosa 'brassicae'. Eur. J. Plant Pathol. 140:841-857. DOI |
8 | Zhang, X., Peng, G., Kutcher, H. R., Balesdent, M.-H., Delourme, R. and Fernando, W. G. D. 2016a. Breakdown of Rlm3 resistance in the Brassica napus-Leptosphaeria maculans pathosystem in western Canada. Eur. J. Plant Pathol. 145:659-674. DOI |
9 | Zhang, X., Peng, G., Parks, P., Hu, B., Li, Q., Jiang, L., Niu, Y., Huang, S. and Fernando, W. G. D. 2016b. Identifying seedling and adult plant resistance of Chinese Brassica napus germplasm to Leptosphaeria maculans. Plant Pathol. 66:752-762. DOI |
10 | Zhang, X., White, R. P., Demir, E., Jedryczka, M., Lange, R. M., Islam, M., Li, Z. Q., Huang, Y. J., Hall, A. M., Zhou, G., Wang, Z., Cai, X., Skelsey, P. and Fitt, B. D. L. 2014. Leptosphaeria spp., phoma stem canker and potential spread of L. maculans on oilseed rape crops in China. Plant Pathol. 63:598-612. DOI |
11 | Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissues. Focus 12:13-15. |
12 | Fernando, W. G. D., Zhang, X. and Amarasinghe, C. C. 2016. Detection of Leptosphaeria maculans and Leptosphaeria biglobosa causing blackleg disease in canola from Canadian canola seed lots and dockage. Plants 5:12. DOI |
13 | Fitt, B. D. L., Brun, H., Barbetti, M. J. and Rimmer, S. R. 2006. World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). Eur. J. Plant Pathol. 114:3-15. DOI |
14 | Ansan-Melayah, D., Balesdent, M. H., Delourme, R., Pilet, M. L., Tanguy, X., Renard, M. and Rouxel, T. 1998. Genes for race-specific resistance against blackleg disease in Brassica napus L. Plant Breed. 117:373-378. DOI |
15 | Fitt, B. D. L., Hu, B. C., Li, Z. Q., Liu, S. Y., Lange, R. M., Kharbanda, P. D., Butterworth, M. H. and White, R. P. 2008. Strategies to prevent spread of Leptosphaeria maculans (phoma stem canker) onto oilseed rape crops in China; costs and benefits. Plant Pathol. 57:652-664. DOI |
16 | Fu, F., Liu, X., Wang, R., Zhai, C., Peng, G., Yu, F. and Fernando, W. G. D. 2019. Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing. Sci. Rep. 9:14600. DOI |
17 | Gan, L., Sun, X., Jin, L., Wang, G., Xu, J., Wei, Z. and Fu, T. 2003. Establishment of math models of NIRS analysis for oil and protein contents in seed of Brassica napus. Sci. Agric. Sin. 36:1609-1613 (in Chinese). DOI |
18 | Howlett, B. J. 2004. Current knowledge of the interaction between Brassica napus and Leptosphaeria maculans. Can. J. Plant Pathol. 26:245-252. DOI |
19 | Kutcher, H. R., Yu, F. and Brun, H. 2010. Improving blackleg disease management of Brassica napus from knowledge of genetic interactions with Leptosphaeria maculans. Can. J. Plant Pathol. 32:29-34. DOI |
20 | Basunanda, P., Radoev, M., Ecke, W., Friedt, W., Becker, H. C. and Snowdon, R. J. 2010. Comparative mapping of quantitative trait loci involved in heterosis for seedling and yield traits in oilseed rape (Brassica napus L.). Theor. Appl. Genet. 120:271-281. DOI |
21 | Larkan, N. J., Lydiate, D. J., Parkin, I. A. P., Nelson, M. N., Epp, D. J., Cowling, W. A., Rimmer, S. R. and Borhan, M. H. 2013. The Brassica napus blackleg resistance gene LepR3 encodes a receptor-like protein triggered by the Leptosphaeria maculans effector AVRLM1. New Phytol. 197:595-605. DOI |
22 | Larkan, N. J., Ma, L. and Borhan, M. H. 2015. The Brassica napus receptor-like protein RLM2 is encoded by a second allele of the LepR3/Rlm2 blackleg resistance locus. Plant Biotechnol. J. 13:983-992. DOI |