• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.477-482
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• 2009

Inhibitory effect of skin and seed of three species grape cultivated in Korea on melanogenesis was investigated. Melanin generation was examined in $\alpha$-Melanin Stimulating Hormone-stimulated B16 cell, mouse melanoma, in the presence of samples. All skin sample did not show the inhibitory effect. Seed extract of Campbell early and Neo Muscat had negative effect on cell viability. When $50{\mu}g/ml$ seed extract of Muscat Bailey A was treated, amount of generated melanin and cell viability were $51.6{\pm}20.5%$ and $90.4{\pm}11.3%$ compared to control, respectively. Seed extract of Muscat Bailey A reduced the tyrosinase protein induced by $\alpha$-Melanin Stimulating Hormone, which suggests that inhibitory effect of seed extract of Muscat Bailey A on melanin is partly due to suppression of tyrosinase that is responsible for melanin production.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.4
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• pp.363-369
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• 2007

The objective of this study was to investigate the variation of Chinese milk vetch(Astragalus sinicus L.; CMV) seed quality after flowering. We tagged individual open flowers of CMV at the day of maximum flowering(11 May) in Seoul, Korea. Seed samples were harvested serially at 15, 20, 25 and 30 days after flowering(DAF). To compare with dried seeds, non-dried seeds were tested immediately after harvest and the remaining seeds were placed at room temperature for 4 weeks. Seed length, 1000 seed weight, moisture content, germination rate(GR), mean germination time(MGT), germination speed(GS), germination performance index(GPI) and physical dormancy rate(PDR) were investigated. Seed length increased to 2.6 mm and 1000 seed weight reached up to 2.2 g until 25 DAF. Seed moisture content dramatically decreased from 20 to 25 DAF. Moisture content of non-dried seed(7.5%) was similar to that of dried seed(5.5%) at 25 DAF. The rate of seed viability reached up to 94% at 25 DAF. In case of dried seed, GR increased up to 39% at 25 DAF whereas GR of non-dried seed varied from 5 to 10%. GS and GPI of dried seed were significantly higher than those of non-dried seed since 25 DAF. PDR of dried seed has decreased since 20 DAF, whereas PDR of non-dried seed has increased. GR, GS and GPI increased as PDR decreased. Our results evidenced that PDR might be one of major factor in variation of seed quality, of which development was completed at 25 DAF.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.4
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• pp.406-412
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• 2014

The influence of rainfall during the ripening stage on pre-harvest sprouting, seed viability, and seed quality was investigated in two Korean rice cultivars, Shindongjin and Hopum. When the rainfall was artificially treated in a greenhouse, HP started to pre-harvest sprouting at three days of rainfall treatment (DRT), but Shindongjin did not show pre-harvest sprouting at 40 DAH treatment and just 0.3~0.8% at 50 DAH, which was much lower than 15.3~25.8% of Hopum in the same treatment. After harvest, the seed germination of Hopum decreased about 10~25% compared to non-treated seeds, but that of Shindongjin decreased much little rate than that of Hopum. The seed longevity tested by accelerated aging decreased with prolonged rainfall period in both cultivars, but the varietal difference was clear; Shindongjin could withstand longer accelerated aging than Hopum. Shindongjin maintained its germination (>50%) ability after 15 days of accelerated aging regardless of the rainfall treatment period and time, but Hopum dropped below 50% germination ability after only 5 days of accelerated aging. In conclusion, rainfall during the ripening stage induced not only pre-harvest sprouting, but also reduced seed quality and longevity during storage, which varied between two cultivars.

• Journal of Environmental Science International
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• v.27 no.10
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• pp.907-923
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• 2018

The aim of this study was to determine the appropriate stockpiling methods for revegetation by comparing the germination status of seed banks before and after preservation for 2 years. Soil temperature in stockpiled topsoil was higher in open treatment and at 1.5 m, whereas soil water content was maintained at lower levels (14.06-19.08%), than those in the control group. The seed banks in stockpiled topsoil had 48 species and 1,559 individuals, among which perennials showed the highest number in terms of life forms, whereas Compositae and Gramineae were dominant in terms of families. Based on seed bank type, persistent seed banks had the highest number of species, while transient seed banks had the highest number of individuals. By stockpiling period, the number of species in the seed bank started to increase after 24 months, while the number of individuals began increasing after 12 months and exceeded that of the control group after 24 months. Regarding the treatment of stockpiling methods, the number of species and individuals in open treatment were closer to those of the control group. When analyzed by height, the number of species and individuals were higher at 0 m, but still lower than those of the control group. A multivariate analysis of variance (MANOVA ) showed that the optimal combination was obtained in open treatment and the number of individuals increased with the lengthening of the stockpiling period.

• Plant Resources
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• v.5 no.2
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• pp.118-123
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• 2002

In order to obtain the genetic information on the quantitative characters of black seeded soybeans, which would be needed to improve selection efficiency for breeding high yielding genotype, 45 varieties of black seeded soybeans collected in Chonnam, Korea were grown and variations of several important characters were observed. Heritability of each observed character, phenotypic and genotypic correlations among the characters and contribution of each yield component on grain yield through path coefficient analysis were estimated. Both number of pods per plant and 100-seed weight showed not only high heritability but also highly significant phenotypic and genotypic correlation with seed yield, and hence it was desirable to select plants with more number of pods per plant and higher 100-seed weight than raise seed yield of black seeded soybeans collected in Chonnam. In addition, number of pods per and 100-seed weight were proved to be the most influential variables on the viability of seed yield by path coefficient analysis. Since these showed the high heritability of number of pods per plant, selection of plants with higher 100-seed weight would be more efficient for breeding high yielding genotype.

• Asian Journal of Turfgrass Science
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• v.2 no.1
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• pp.70-77
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• 1988

In order to determine and to reduce the differences between biochemical test method for the seed viability with T.T.C. (2, 3, 5. Triphenyl tetrazolium Chloride) reagent and germinator method, the topographical diagram of red colored formazan was carried out in 15-16 parts differences. From this resulted information, the classification of the typical staining reaction given for 3 species were derived into 3-4 parts for the germinable in normal seedling, the same as the following results. 1. Corn (Gram inaceae) * Entier embryo stained in bright red color. * Both extremities of scutellum unstained * Both extremities of scuttlium, coleorhiza and non-critial portions of radicle unstained. 2. Soybean (Leguminosae) *Seed completely stained in red color. *Minor unstained areas on cotyledons. *Extreme tip of radicle unstained; minor unstained areas on cotyledons 3. Radish (Cruciferae) *Seed completely stained. *Minor unstained areas on cotyledons. *Outer cotyledon mostly unstained: inner cotyledon completely stained Extreme tip of radicle unstained: large portion of outer cotyledon unstained.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.324-328
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• 2008

One hundred-eighty extracts of soil collected from ginseng (Panax ginseng C.A. Meyer) fields were subjected to lettuce germination test, electrolyte leakage, cell viability and antioxidant enzyme activity test. Regardless of various cultivation periods, there was no significant difference in soil pH, the content of organic matter and available phosphate in ginseng fields. Based on lettuce seed germination test, six soil extracts showing inhibition of germination and/or seedling growth were selected for further study. Selected soil extracts markedly inhibited cell viability of ginseng cultured cells but leakage of electrolytes were not affected by the treatment. Enzyme activity of superoxide dimutase in ginseng cultured cells was not affected by the treatment with the soil extracts. However, those of peroxidase and catalase were significantly inhibited by the treatment with soil extracts which showed inhibition of lettuce seed germination and seedling growth.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.655-666
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• 2022

We assessed the persistence of eight major cruciferous crops-leaf mustard, oilseed rape, cabbage, broccoli, cauliflower, Chinese cabbage, turnip, and radish-growing in the field. In the first part of our experiment, we tested the viability of seeds that had been buried at two different soil depths for up to 16 months. We then broadcast seeds over the soil surface and left them undisturbed to investigate the survivorship of the resultant plants over two years. Seed viability was significantly affected by plant taxa and burial depth, but not substantially affected by the duration of burial. Although seeds of leaf mustard had the greatest viability among all crops examined here, the viability rates were significantly lower at 2 cm depth than at 15 cm. Seeds of leaf mustard, oilseed rape, broccoli, turnip, and Chinese cabbage remained viable throughout the 16-month period. A study of plant demography revealed that only leaf mustard and oilseed rape succeeded in producing seeds and overwintering in the undisturbed field. However, neither of those species competed well with other plants long-term and their overall growth and survival rates declined during the evaluation period. In addition, insect herbivory severely decreased the growth of all of these crops. Our results suggest that populations of leaf mustard and oilseed rape do not tend to persist in the field for more than a few years without disturbance and external seed inputs.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.163-168
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• 2012

An optimization in vitro seed germination was established by using triphenol tetrazolium chloride (TTC) test, which has been known as two rare orchids (Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama) in Jeju island. We have established proper NaOCl treatment for in vitro germination of G. verrucosa and H. sikokiana through TTC test. In the case of H. sikokiana, seed viability through TTC test was high with 95% in control. However, NaOCl 1% treatment for 30 minutes showed the highest embryo swelling rate to seed viability. Likewise, swelling formation of embryos, diameter of embryos, protocorm formation and diameter of protocorms of G. verrucosa was 87%, $59{\mu}m$, 91% and $138{\mu}m$ through NaOCl 1% treatment for 30 minutes. This result will be applied on the basic information for improving in vitro seed germination rate of G. verrucosa and H. sikokiana.

• Journal of Biosystems Engineering
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• v.37 no.6
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• pp.411-419
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• 2012

Purpose: Nondestructive evaluation of seed viability is a highly demanded technique in the seed industry. In this study, hyperspectral imaging system was used for discrimination of viable and non-viable radish seeds. Method: The spectral data with the range from 400 to 1000 nm measured by hyperspectral reflectance imaging system were used. A calibration and a test models were developed by partial least square discrimination analysis (PLS-DA) for classification of viable and non-viable radish seeds. Either each data set of visible (400~750 nm) and NIR (750~1000 nm) spectra and the spectra of the combined spectral ranges were used for developing models. Results: The discrimination accuracy of calibration was 84% for visible range and 76.3% for NIR range. The discrimination accuracy of test was 84.2% for visible range and 75.8% for NIR range. The discrimination accuracies of calibration and test with full range were 92.2% and 92.5%, respectively. The resultant images based on the optimal PLS-DA model showed high performance for the discrimination of the nonviable seeds from the viable seeds with the accuracy of 95%. Conclusions: The results showed that hyperspectral reflectance imaging has good potential for discriminating nonviable radish seeds from massive amounts of viable seeds.