• Asian-Australasian Journal of Animal Sciences
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• v.20 no.8
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• pp.1196-1200
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• 2007

Eight feeds (mixture of wheat straw and oil seed cakes in 3:1 ratio) were evaluated for methane emission and fermentation pattern with buffalo rumen liquor as inoculum in an in vitro gas production test. The cakes tested were groundnut cake (GNC), soybean cake (SBC), mustard seed cake (MSC), cotton seed cake (CSC), karanj seed cake expeller extracted (KCEE), karanj seed cake solvent extracted (KCSE), caster bean cake expeller extracted (CBCEE) and caster bean cake solvent extracted (CBCSE). The gas production (ml/g dry matter) was significantly higher with SBC and MSC followed by CSC, GNC, KCSE, KCEE, CBCSE and was the lowest with CBCEE. Methane emission was significantly lower with KCEE, KCSE, CBCEE, CBCSE (20.32- 22.43 ml/g DM) than that with SBC, GNC, CSC (27.34-31.14 ml/g DM). Mustard seed cake was in-between the two groups of oil cakes in methane production. In vitro true digestibility was highest with SBC followed by GNC, CSC, MSC, KCSE, KCEE, CBCSE and CECEE. Ammonia nitrogen level was positively correlated with the amount of protein present in the cake. Total holotrich protozoa were significantly higher with SBC, whereas, large spirotrich protozoa tended to be lower than with other cakes. The counts of small spirotrich and total protozoa were similar with all the cakes. Total volatile fatty acid production and acetate to propionate ratio were significantly higher with SBC and significantly lower with KCEE as compared to the other cakes. Among the conventional oil cakes tested in the present experiment (GNC, SBC, MSC and CSC), mustard seed cake-based feed produced the minimum methane without affecting other fermentation characteristics adversely.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.319-324
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• 2010

Phorbol ester extraction was carried out from Jatropha curcas seed cake, a by-product from the biodiesel fuel industry. Four repeated extractions from 5 g of J. curcas seed cake using 15 ml of 90% (v/v) ethanol and a shaking speed of 150 rpm gave the highest yield of phorbol esters. The ethanolic extract of J. curcas seed cake showed antifungal activities against important fungal phytopathogens: Fusarium oxysporum, Pythium aphanidermatum, Lasiodiplodia theobromae, Curvularia lunata, Fusarium semitectum, Colletotrichum capsid, and Colletotrichum gloeosporioides. The extract contained phorbol esters mainly responsible for antifungal activities. The extract could therefore be used as an antifungal agent for agricultural applications.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.966-972
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• 2005

A study to compare the effects of supplementing Delonix elata, Grewia similis, Tamarindus indica and sunflower seed cake on intake and growth rate of dual-purpose goats fed low quality Buffel grass (Cenchrus ciliaris) hay was carried out. Twenty-eight male goats aged five to seven months (mean weight 12.93${\pm}$3.94 kg) were randomly allocated to four dietary groups in a completely randomised design. The diets were hay plus Grewia similis, hay plus Delonix elata, hay plus Tamarindus indica and hay plus sunflower seed cake. All diets were supplemented with maize bran. The experimental period was 90 days. Voluntary dry matter intake of the supplements was higher for Tamarindus indica (275.5 g/day) and Grewia similis (201.8 g/day) and lowest for sunflower seed cake (81g/day). Goats supplemented with Grewia similis had the highest hay intake (183.8 g/day) while those supplemented with sunflower seed cake had the lowest hay intake (98.9 g/day). Animals fed browse supplements gained significantly more weight (p<0.001) than those with sunflower seed cake. There were no significant differences in live weight change between goats fed the different browses. However, those fed Tamarindus indica gained an average of 20.79 g/d which was slightly higher than the gains for those on Grewia similis and Delonix elata while those fed sunflower seed cake lost weight. Correspondingly, goats supplemented with browse leaf meals had higher feed conversion ratios than those supplemented with sunflower seed cake and required 23.91 to 35.06 g DM of feed to produce one g of weight gain per day. In a separate study, the DM disappearance pattern indicated that Grewia similis and Delonix elata were highly degradable compared to Tamarindus indica. At 24 h of incubation, DM degradability was 627, 588 and 345 g/kg DM for Grewia similis, Delonix elata and Tamarindus indica, respectively. In another study in vivo DM digestibility ranged from 46.1% (for hay alone) to 56.2% (for hay plus Grewia similis). It was concluded that the addition of Tamarindus indica, Grewia similis and Delonix elata leaf meals to Cenchrus ciliaris hay resulted in increased total DM intake, in vivo digestibility and growth rate. Therefore, leaf meals of indigenous browses particularly Tamarindus indica and Grewia similis could be used as supplementary feeds for small ruminants grazing on poor quality roughages during the dry season rather than use of expensive, less effective and intermittently available sunflower seed cake.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.997-1003
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• 2006

An experiment with twenty crossbred goat kids (male) of 2-3 months old, weighing about 12 kg was conducted to study the effect of feeding formaldehyde treated rape seed oil cake based diet supplemented with molasses on growth rate and histopathological changes of different organs. Goats were randomly divided into four groups of 5 animals each and were individually fed for a period of 120 days. The animals in group I (URC) and II (URCM) were fed concentrate mixture (CM-I) containing untreated rape seed oil cake (30%) while, the animals in group III (TRC) and IV (TRCM) were offered concentrate mixture (CM-II) containing formaldehyde treated rape seed oil cake. Further, molasses as energy source was additionally supplemented with the concentrate mixture at the rate of 8% of concentrate mixture on dry matter basis to animals in group II and IV. All the animals were maintained on roughage (Berseem hay:wheat straw = 2:1) and concentrate in 50:50 ratio. Average daily gain (g/d) of animals in group IV was significantly (p<0.05) higher than that in group I., but at par with group II and III. Feed conversion efficiency was also significantly (p<0.05) higher in group IV (10.14) than group I and II but at par with group III. The growth rate however increased by 50.2% in group IV showing more consistency in maintaining highest growth rate due to better balance of nutrients. At the end of four months of feeding trial, two animals from each group were sacrificed for histopathological study of different organs. Significant histopathological changes in liver, heart, lungs tissue of animals fed untreated rape seed oil cake diet were recorded which were totally absent in the organ of animals fed formaldehyde treated cake. The liver tissue of goats receiving control diet (containing untreated rape seed oil cake) were found to be associated with engorged central vein and blood vessels. Hepatocytes were swollen, pale and degenerated with cellular infiltration and fibrosis of portal areas. The muscles of heart were found to have intermyofibral edema. Emphysema accompanied by dilated and ruptured alveoli was also recorded in lung tissue. However, histopathological examination of various tissues of goats fed formaldehyde treated cake diet did not exhibit any degenerative changes. Additional supplementation of molasses with or without treated cake diet, apparently did not have any significant effect on ameliorating the above degenerative changes.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.11
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• pp.1531-1539
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• 2009

This study compared the nutritional value of canola (B. napa) and mustard (B. hirta) press cakes obtained from the biodiesel industry as ingredients for use in diets fed to broiler chickens. A total of 210, one-day old, male broiler chicks were randomly assigned to one of seven dietary treatments. The control diet was based on wheat and soybean meal and contained 15% canola meal. For the experimental diets, 5, 10 or 15% of the canola meal was replaced with an equal amount of either canola or mustard biodiesel press cake. Dry matter and neutral detergent fiber digestibility were significantly higher for birds fed diets containing either canola or mustard biodiesel press cake compared with canola meal. Dry matter and neutral detergent fiber digestibility of the canola biodiesel press cakes was higher than the mustard biodiesel press cakes. Ether extract digestibility and nitrogen retention were significantly higher for birds fed canola biodiesel press cake compared with canola meal and mustard biodiesel press cake. Body weight gain and feed intake did not differ between birds fed canola or mustard biodiesel press cake and canola meal. In addition, there was no significant difference in body weight gain or feed intake between birds fed diets containing canola or mustard biodiesel press cake. Feed conversion was significantly improved for birds fed either canola or mustard biodiesel press cake compared with canola meal. Mortality was unaffected by treatment. Since the performance of broilers fed canola biodiesel press cakes was essentially the same as that of broilers fed canola meal, it is difficult to justify a premium to be paid for canola biodiesel press cake over that paid for canola meal. In addition, there was no difference in the performance of broilers fed biodiesel press cake obtained from canola or mustard seed. As mustard seeds are generally available at a lower price than canola seed, there may be some incentive to use mustard rather than canola seed for producing biodiesel press cake for use in poultry production.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.720-728
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• 2000

The different products of neem (Azadirachta indica) are utilized for variety of purposes in industry, health and animal agriculture in the Indian subcontinent. The cake from seeds after oil extraction is a good source of nutrients (CP: 35-38%; EE: 4.5-5.5%; CF: 12-15%; Ca: 0.75%; P: 0.45% on DM), and in particular, the one out of its kernel is proteinaceous and is relatively balanced in its amino acid and mineral profile. But the cake is toxic and bitter to taste owing to triterpenoids (nimbin, salannin, azadirachtin), which restricts its safe inclusion in livestock diet. Several feeding trials with raw cake have revealed poor palatability and adverse performance among different categories of livestock and poultry. Internal organ changes included histological alteration in intestine, liver, kidney and distruption of spermatogenesis and ovarian activity. Ruminants appears to tolerate reasonably higher levels of the cake and to a limited low levels of dietary inclusion also proved to be tolerable in monogastric farm animals. Debitterization through solvent (hexane, ether) extraction, water washing, alkali (NaOH, 1.5, 2.5 or 3%, wt/wt) soaking and urea (1.5 or 3%, wt/wt) - ammoniation have been tried with appreciable success in improving the palatability and nutritive value of the cake. For enhanced utilization, decortication of neem seeds is to be done effectively at industrial level with maximum oil recovery. The resultant proteinaceous kernel by-product could be a cheaper unconventional protein supplement after suitable processing.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1273-1277
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• 2002

The effect of urea treatment on chemical composition of neem seed kernel cake (NSKC) was investigated by soaking the cake in 2.1% urea solution (1.2 l $kg^{-1}$ NSKC) for five days. The effect on utilisation of nutrients by replacing groundnut cake (GNC) (30%) with urea-treated neem seed kernel cake (UTNSKC) (33%) in a concentrate mixture fed to meet 70% of the protein requirements of lambs (8 males and 8 females), was monitored in a digestibility study. Following urea treatment of NSKC only 9.5% of urea was hydrolysed and the crude protein content of the cake was increased by 6.65%. The tannin content in depulped neem seeds was 37% catechin equivalent. Whereas feeding UTNSKC had no effect on intake of dry matter (72.5 vs 66.3 g/kg $BW^{0.75}day^{-1}$) and digestibility of crude fibre (41.3 vs 43.4%), the cake depressed (p<0.01) the percent digestibility of dry matter (63.7 vs 70.2), crude protein (63.2 vs 70.2), nitrogen free extract (73.8 vs 80.5) and gross energy (64.3 vs 69.1). Digestibility of ether extract (75.8 vs 70.9%) was higher (p<0.05) in animals offered UTNSKC. The nutritive value of the composite ration consumed by lambs offered UTNSKC was lower (p<0.01) in terms of total digestible nutrients (64.7 vs 70.2%) and digestible energy (2.8 vs 3.0 Kcal/g DM). Intake of digestible energy (199.8 vs 194.1 Kcal/kg $BW^{0.75}day^{-1}$) and retention of nitrogen (7.53 vs 8.23 g $day^{-1}$) and calcium (2.12 vs 1.84 g $day^{-1}$) were comparable on the 2 rations. Animals fed UTNSKC retained less (p<0.01) phosphorus (0.37 vs 1.05 g $day^{-1}$). The results indicate that urea treatment increased the protein level of NSKC whereas feeding the treated cake as a replacement of GNC, lowered the digestibility of nutrients and retention of phosphorus.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.416-420
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• 2002

Various products of karanj (Pongamia glabra) are utilized for industrial, health and animal agriculture applications in the Indian subcontinent. Despite a rich source of protein (CP, 28-34%), karanj cake was found to be slightly bitter in taste and toxic owing to the presence of flavonoid (Karanjin), restricting its safe inclusion in the livestock diets. Feeding trials with raw cake revealed its poor palatability and adverse performance among different categories of livestock including poultry. The present study was, therefore, aimed to detoxify karanj cake by various physico-chemical methods like solvent extraction, water washing, pressure cooking and alkali and acid treatments. The level of residual karanjin in raw and variously processed cake was quantified using high performance liquid chromatography (HPLC). The raw expeller karanj cake was found to contain about 0.19% of karanjin. Though a non-polar solvent, soxhlet extraction of expeller pressed cake with petroleum ether drastically reduced karanjin content (0.01%). Soaking of cake for 24 h in 1% NaOH (w/w) solution was found to reduce karanjin to a major extent with little further benefit by increasing alkali level. Milder alkalies like lime and fertilizer grade urea reduced the karanjin levels marginally. Similar was the case with mineral acids such as HCl and glacial acetic acid. It was, therefore, concluded that solvent extraction of karanj seeds would be the best method of detoxification as well as for more recovery of oil and karanjin.

• Korean Journal of Food Science and Technology
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• v.7 no.3
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• pp.120-127
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• 1975

Despite the extensive study of protein effect to man and domestic animals, protein effect of sunflower seed oil cake to the growth and value of erythrocyte in rats is still remained to be investigated. This study is to investigate the effect of protein of sunflower seed oil cake to the rat growth and value of erythrocyte as well as the liver weight, heart, kidneys and spleen of rats. Sixty to seventy-days-old, male Sprague-Dawley rats weighing 50-60g, were used in this experiment and 36 rats allotted to 4 groups: standard, 30% cake, 20% cake and low protein. The standard group was fed for 7 weeks on the ration supplemented with 17% casein, 30% and 20% cake groups with sunflower seed oil cake by 30% and 20%, respectively, and low protein group without any supplementation. To observe value of erythrocyte, blood was taken from heart. Value of erythrocyte, hemoglobin and hematocrit value were determined by usual method and oxygen consumption by the method of Watts and Gourley. The result of this experiment suggests that it is recommendable to supplement deficient amino acids in animal.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.30-34
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• 2001

The expediency of promoting rabbit production on underutilized by-product feedstuffs prompted the current investigation of caecal fermentation pattern, blood biochemical profile and histopathological changes of vital organs in 48 broiler rabbits fed diets containing 0, 5, 10 and 20% level of neem seed kernal cake (NSKC) for 6 weeks and subsequently sacrificed. The NSKC incorporation in the diet did not exert any adverse effect on caecal fermentation although the weight of caecum and its contents was significantly (p<0.01; p<0.05) lower in rabbits fed the diet containing 20% NSKC. Except for blood glucose concentration, none of the blood biochemical constituents (serum urea nitrogen, creatinine, total protein, haemoglobin) and the activities of different enzymes (alanine aminotransferase, asparatate aminotransferase, alkaline phosphatase) varied significantly due to NSKC feeding. A histopathological examination of the vital organs (liver, kidney, heart, spleen, lungs, intestine and stomach) revealed a variable degree of villus atrophy in the intestine and degenerative changes in the liver and tubular epithelium of kidney in some rabbits when NSKC was fed at levels above 10%.