• 제목/요약/키워드: sectioning method

검색결과 67건 처리시간 0.028초

형광 공초점 주사 현미경의 측정 강도 향상을 위한 반사 광학계의 제안 및 설계 (Proposal and design of reflecting optical system to improve detection intensity in fluorescence confocal scanning microscopy)

  • 강동균;서정우;권대갑
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2002년도 춘계학술대회 논문집
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    • pp.187-190
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    • 2002
  • Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.

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알루미늄 합금 용접부의 피로균열성장거동에 관한 파괴력학적 연구 (A Fracture Mechanics Study on the Fatigue Crack Growth Behaviors in Aluminum Alloy Weldments)

  • 차용훈
    • 한국생산제조학회지
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    • 제5권3호
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    • pp.31-39
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    • 1996
  • The objective of this study is to investigate the influence of welding residual stresses on the fatigue crack growth behavior of cracks located transverse to the weld bead. For this purpose, G. T. A (Gas Tungsten Arc) welding was performed on hte Al alloy 1100-O plate and the same initial crack is made on HAZ(Heat Affected Zone), weld metal and base meta respectively. Specimens were used CT(Compact Tension) specimens. Initial welding residual stresses were measured by using strai gage sectioning method. All specimens were tested under constant amplitude load with stress ratio R=0.1, It is possible to predict fatigue crack growth behaviors and the fatigue life, using numerical analysis together with distribution of initial residual stress and the values of C and m obtained from $da/dN-{\Delta}K$

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열차거리에 따른 절연구간 무접전 자동 통과시스템의 검토 (Investigation of Automatic Power Switching System in Dead Section Depending on Train Mileage)

  • 김강회;한문섭;장동욱;창상훈;이재호
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2010년도 춘계학술대회 논문집
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    • pp.1878-1884
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    • 2010
  • Currently in Korea, neutral sections in front of substations and sectioning posts are greatly divided into AC/DC sections and AC/AC sections. Considering catenary damage and safety issues trains pass crossover by coasting driving at notch-off. However in cases of Japan, China, or Taiwan, the usage of automatic crossover system makes it possible to pass at notch-on, enhancing high-speed railway operation for efficiency, and stability. These are the purposes of developing automatic power crossover system in neutral sections. This paper introduces two methods to detect the position of a train required to activate automatic crossover systems in neutral sections. The optimal method is expected in terms of the distance of neutral section.

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Tannic acid를 이용한 전자현미경 (TEM) 염색효과 (Electron Microscopic Stain Effect by Tannic acid)

  • 윤철종;한정연;김철우;지제근
    • Applied Microscopy
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    • 제24권2호
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    • pp.37-47
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    • 1994
  • Using mouse tissue, we studied electron opacity effect of tannic acid for transmission electron microscopic staining. Tannic acid-glutaraldehyde in 0.1M phosphate buffer was used as a fixative. To compare with this we have tested another method consisting of heavy metal staining after treatment of tannic acid in sodium tetraborate (borax) on glutaraldehyde-fixed sections. We have achieved equally consistent electron opacity in both methods. The elastin, collagen, basal lamina of skin and gap junctions of the epithelial cells gave excellent results, while it was good for glycogen, cilia, and plasma. Also fat cells and lipid droplets gave good preservation when tannic acid was added in the fixative. However, prolonged fixation in tannic acid-added fixative was hazardous for further processing, i.e., sectioning problem and deep electron opacity background.

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AI 합금 용접부의 잔류응력 및 피로강도 해석 (Analysis of the Residual Stresses and Fatigue Strenth in Aluminum Alloy Weldments)

  • 차용훈
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 1996년도 춘계학술대회 논문집
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    • pp.177-182
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    • 1996
  • The objective of this thesis is to investigate the influence of welding residual stresses on the fatigue growth behavior of welding residual stresses on the fatigue growth behavior of cracks located transverse to the weld bead. For this purpose, G.T.A. (Gas Tungsten Arc) welding was performed on the Al. Alloy 1100-0 plate and the same initial crack is made on HAZ(Heat Affected Zone), weld metal and base metal respectively. C.T.(Compact Tension) specimens were used as experimental material. Initial welding residual stresses were measured by using strain gage sectioning method. All specimens were tested under constant amplitude load with stress ratio R=0.1

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패턴 빔을 이용한 BGA 단차 측정 (BGA Height Measurement Using Pattern Beam)

  • 신상훈;유영훈
    • 한국광학회지
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    • 제20권6호
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    • pp.361-365
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    • 2009
  • 본 연구에서는 패턴 빔을 이용하여 표면이 거친 물체의 단차를 비접촉 방식으로 측정 하는 방법에 대하여 연구하였다. 본 연구에 사용된 방법은 장치가 매우 간단하고 스페클 노이지가 영상에 미치는 영향이 작아 재현성 면에서 매우 우수하였다. 특히 기준면에 문양이 없는 경우에는 기존의 자동 초점 측정 장치로는 측정하기 어려우나, 본 연구 방법에 의해 측정이 가능함을 보였다. 그리고 측정 시간이 매우 짧고 재현성이 좋으며 장치가 간단하여 산업 현장에서 응용하기 적절한 방법이다.

고속영상처리에 의한 레일형상측정시스템 개발 (Development of Rail Profile Measurement System using High Speed Laser Image Processing)

  • 문철이;김만철
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2006년도 추계학술대회 논문집
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    • pp.1320-1325
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    • 2006
  • Railway track supports and guides train and it directly affects ride quality and safety. To maintain track quality, regular maintenance activity is essential but it degrades track usage since maintenance activity occupies the track during the work. To achieve maximum track efficiency, track inspection should be performed at commercial running speed. In this paper, we designed and developed high speed rail profile measurement system using laser light sectioning method with line laser generator, digital camera and DSP based image processing system. The measurement system can supports rail profile measurement at the speed of 300 Km/h that can be applied to KTX.

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감마선을 이용한 소결 전 Fe/Ni 분말야금 판재의 밀도측정에 관한 연구 (Studies on Density Measurement of Green Fe/Ni P/M Sheet Using ${\gamma}-ray$)

  • 조경식;이종오;이상율;이주석
    • 비파괴검사학회지
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    • 제12권3호
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    • pp.7-11
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    • 1992
  • Accurate measurement of green density of compacted part in the powder metallurgy industry is rather fundamental but extremely important process that decide the quality of the sintered part. In case of green sheet P/M product, the green density as well as the distribution of the density must be examined for the same reasons. Currently in most cases, density measuring process is being performed applying conventional Archimedes principles. However this method is not only time-consuming but also often inaccurate because of the inherent nature of the process, such as part sectioning, closing of surface porosity with wax and weighing in air and in water. Therefore, it is necessary to develop a faster and more accurate method to measure the density of green sheet P/M product. In this work, a nondestructive density measurement device using gamma-ray absorption principles was constructed and the optimum condition for measuring green density of P/M sheet and its distribution was sought. The results showed that this method was very effective in terms of measuring time and accuracy.

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Cryo-Methods와 Cryo-SEM을 이용한 Cyanobacteria, Synechocystis sp. PCC 6803 미세구조 관찰 (Observations of the Cyanobacteria Synechocystis sp. PCC 6803 using Cryo-Methods and Cryo-SEM)

  • 이은주;문윤정;오현우;김수진;정영호;권희석;김윤중
    • Applied Microscopy
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    • 제39권1호
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    • pp.65-72
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    • 2009
  • Cryo-SEM은 수분을 함유하거나 액상 시료를 동결된 상태로 관찰하는 방법으로 rapid cooling, fracturing, sectioning, etching, coating과 같은 다소 복잡한 여러 과정의 전처리(Cryo-methods) 로 구성된다. 각 과정에서는 분석 목적이나 시료 특성(예: 시료크기, 물 함량 등)을 고려하여 최적의 조건을 설정하여야 한다. 본 연구에서는 cryo-SEM을 이용하여 남세균 (cyanobacteria, Synechocystis sp. PCC 6803)의 표면 및 내부 구조 관찰을 위한 etching time과 시료 처리에 관하여 살펴보았고 cryo-methods로 처리한 후 cryo-SEM으로 관찰한 이미지를 화학 고정한 일반 SEM(CSEM) 결과와 비교하였다. 관찰 결과, 화학 고정한 CSEM에서는 Synechocystis sp. PCC 6803 표면에 존재하는 pili가 잘 관찰되지 않았으며 파단된 내부 구조의 이미지를 얻을 수 없었으나 cryo-methods/cryo-SEM에서는 세포 표면의 pili 및 membrane의 형태를 관찰할 수 있었다. 또한 수화 상태에 따라 구조변형이 일어나는 biofilm의 구조도 관찰할 수 있었다. 이러한 결과로부터 cryo-methods/cryo-SEM은 수분을 함유하는 의생물 시료의 형태 구조를 분석하는 유용한 방법으로 사료된다.

FINITE ELEMENT ANALYSIS AND MEASUREMENT ON THE RELEASE OF RESIDUAL STRESS AND NON-LINEAR BEHAVIOR IN WELDMENTS BY MECHANICAL LOADING(I) - EXPERIMENTAL EXAMINATION -

  • Jang, Kyoung-Bok;Yoon, Hun-Sung;Cho, Sang-Myoung
    • 대한용접접합학회:학술대회논문집
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    • 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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    • pp.372-377
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    • 2002
  • Residual stress by welding should be reduced because that decreases the reliability on strength of welded structure. The reason is that the total stiffness of structure decreases by non-linear behavior of weldment under external load. The release of residual stress by mechanical loading and unloading is often performed in the fabrication of box structure for steel bridge. The proper degree of loading and unloading is significant at release method of residual stress by mechanical loading because that degree is changed by material and geometric shape of welded structure. Therefore, the simulation model that could exactly analyze the release of residual stress by mechanical loading is to be necessary. This simulation model should be established on the based of variable and accurate measurement data. In this study, the non-linear behavior of weldments under external loading and unloading, such as the decrease and increase of structure stiffness, was investigated by monitoring of nominal stress and strain. Tensile loading and unloading test under variable load was performed and the proper degree of stress relaxation was measured by sectioning technique using strain gauge.

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