• 제목/요약/키워드: secondary structure of ITS

검색결과 345건 처리시간 0.019초

경희궁 궁원구성의 특징 (Configuration of the Royal Garden in Gyeonghuigung Palace)

  • 백지성;김민선;조태동
    • 한국환경과학회지
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    • 제25권12호
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    • pp.1673-1688
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    • 2016
  • Gyeonghuigung palace is an important site as it was the secondary palace in Hanyang from the $17^{th}$ century to the mid-$19^{th}$ century. Its original state was lost because a number of buildings were damaged and the palace area was reduced. The entire palace can be seen only in "Seogwoldoan" a drawing of the western palace. With "Seogwoldoan" to show the complete configuration of Gyeonghuigung palace in detail as the main data and other relevant data used as supplementary sources, the aim of this work was to identify the figurative features around the plantings and the space configuration of the royal garden in Gyeonghuigung palace. The space configuration observed in the royal gardens in Gyeonghuigung palace deviated from the norms and strict systems of royal palaces. The free and uninhibited configuration of the royal garden was allowed in Gyeonghuigung palace because it was built as a secondary palace in a lower hierarchy. Furthermore, a royal garden characterized by unique and unlimited planting was created, with several spaces acting as both royal garden and rear garden as the rear gardens could not be separately configured due to the narrow space of Gyeonghuigung palace.

$LiNi_{0.85}Co_{0.15}O_2/MPCF$전지의 충방전 특성 (Charge-discharge behaviour of $LiNi_{0.85}Co_{0.15}O_2>/MPCF$ cell)

  • 김상필;조정수;박정후;윤문수
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1998년도 추계학술대회 논문집
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    • pp.25-28
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    • 1998
  • Lithiated cobalt and nickel oxides are becoming very attractive as active cathode materials for secondary lithium ion secondary battery. $LiCoO_2$ is easily synthesized from lithium cobalt salts, but has a relatively high oxidizing potential on charge. LiNiOz is synthesized by a more complex procedure and its nonstoichiometry significantly degraded the charge-discharge characteristics. But $LiNiO_2$ has a lower charge potential which increases the system stability. Lithiated cobalt and nickel oxides are iso-structure which make the preparation of solid solutions of $LiNi_{1-x}Co_xO_2$ for O$LiCoO_2 and LiNiO_2$ electrode. The aim of the presentb paper is to study the electrochemical behaviour, as weU as the possibilities for practical application of layered Iithiated nickel oxide stabilized by $Co^{3+}$ substitution as active cathode materials in lithium ion secondary battery.

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Discovery of novel Nodosilinea species (Cyanobacteria, Nodosilineales) isolated from terrestrial habitat in Ryukyus campus, Okinawa, Japan

  • Handung Nuryadi;Shimpei Sumimoto;Shoichiro Suda
    • ALGAE
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    • 제39권2호
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    • pp.59-74
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    • 2024
  • Terrestrial cyanobacteria are extremely diverse. In urban areas, they can be found as black stains on the surface of building walls, stone monuments, or man-made structures. Many of the terrestrial cyanobacteria are still understudied. To expand knowledge of terrestrial cyanobacterial diversity, a polyphasic characterization was performed to identify 12 strains isolated from campus of University of the Ryukyus, Okinawa, Japan. Multigene phylogenetic analyses based on 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) region showed that the isolated strains formed two independent subclades within Nodosilinea, and were distantly related to all described Nodosilinea species. The 16S-23S rRNA ITS secondary structures showed variations for D1-D1' and Box B domain, while V3 domain was almost identical among entire species of Nodosilinea, including the studied strains. In addition, a unique morphological character, i.e. forming nodule or spiral shape, was also observed in certain studied strains. According to polyphasic characterization, Nodosilinea coculeatus sp. nov. and Nodosilinea terrestrialis sp. nov., were proposed as two new species of terrestrial cyanobacteria from Okinawa.

A Novel Switched Capacitor High Step-up dc/dc Converter Using a Coupled Inductor with its Generalized Structure

  • Hamkari, Sajjad;Moradzadeh, Majid;Zamiri, Elyas;Nasir, Mehdi;Hosseini, Seyed Hossein
    • Journal of Power Electronics
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    • 제17권3호
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    • pp.579-589
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    • 2017
  • In this study a new high step-up dc-dc converter is presented. The operation of the proposed converter is based on the capacitor switching and coupled inductor with a single active power switch in its structure. A passive voltage clamp circuit with two capacitors and two diodes is used in the proposed converter for elevating the converter's voltage gain with the recovered energy of the leakage inductor, and for lowering the voltage stress on the power switch. A switch with a low $R_{DS}$ (on) can be adopted to reduce conduction losses. In the generalized mode of the proposed converter, to reach a desired voltage gain, capacitor stages with parallel charge and series discharge techniques are extended from both sides of secondary side of the coupled inductor. The proposed converter has the ability to alleviate the reverse recovery problem of diodes with circuit parameters. The operating principle and steady-states analyses are discussed in detail. A 40W prototype of the proposed converter is implemented in the laboratory to verify its operation.

Seven new species of two genera Scalarispongia and Smenospongia (Demospongiae: Dictyoceratida: Thorectidae) from Korea

  • Kim, Young A;Lee, Kyung Jin;Sim, Chung Ja
    • Journal of Species Research
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    • 제9권2호
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    • pp.147-161
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    • 2020
  • Seven new species of two genera Scalarispongia and Smenospongia (Demospongiae: Dictyoceratida: Thorectidae) are described from Gageo Island and Jeju Island, Korea. Five new species of Scalarispongia are compared to nine reported species of the genus by the skeletal structure. Scalarispongia viridis n. sp. has regular ladder-like skeletal pattern arranged throughout the sponge body and has pseudo-tertiary fibres. Scalarispongia favus n. sp. is characterized by the honeycomb shape of the surface and is similar to Sc. flava in skeletal structure, but differs in sponge shape. Scalarispongia lenis n. sp. is similar to Sc. regularis in skeletal structure but has fibers that are smaller in size. Scalarispongia canus n. sp. has irregular skeletal structure in three dimensions and ladder-like which comes out of the surface and choanosome. Scalarispongia subjiensis n. sp. has pseudo-tertiary fibres and its regular ladder-like skeletal pattern occurs at the choanosome. Two new species of Smenospongia are distinguished from the other 19 reported species of the genus by the skeletal structure. Smenospongia aspera n. sp. is similar to Sm. coreana in sponge shape but new species has rarely secondary web and thin and thick bridged fibres at near surface. Smenospongia mureungensis n. sp. has very simple skeletal structure.

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

  • Tawong, Wittaya;Pongcharoen, Pongsanat;Pongpadung, Piyawat;Ponza, Supat;Saijuntha, Weerachai
    • ALGAE
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    • 제37권1호
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    • pp.1-14
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    • 2022
  • Cyanobacteria are distributed worldwide, and many new cyanobacterial species are discovered in tropical region. The Nostoc-like genus Amazonocrinis has been separated from the genus Nostoc based on polyphasic methods. However, species diversity within this genus remains poorly understood systematically because only one species (Amazonocrinis nigriterrae) has been described. In this study, two novel strains (NUACC02 and NUACC03) were isolated from moist rice field soil in Thailand. These two strains were characterized using a polyphasic approach, based on morphology, 16S rRNA phylogenetic analysis, internal transcribed spacer secondary structure and ecology. Phylogenetic analyses based on 16S rRNA gene sequences confirmed that the two novel strains formed a monophyletic clade related to the genus Amazonocrinis and were distant from the type species A. nigriterrae. The 16S rRNA gene sequence similarity (<98.1%) between novel strains and all other closely related taxa including the Amazonocrinis members exceeded the cutoff for species delimitation in bacteriology, reinforcing the presence of a new Amazonocrinis species. Furthermore, the novel strains possessed unique phenotypic characteristics such as the presence of the sheath, necridia-like cells, larger cell dimension and akinete cell arrangement in long-chains and the singularity of D1-D1', Box-B, V2, and V3 secondary structures that distinguished them from other Amazonocrinis members. Considering all the results, we described our two strains as Amazonocrinis thailandica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.

Secalonic acid D; A Cytotoxic Constituent from Marine Lichen-derived Fungus Gliocladium sp. T31

  • Ren, Hong;Tian, Li;Gu, Qianqun;Zhu, Weiming
    • Archives of Pharmacal Research
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    • 제29권1호
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    • pp.59-63
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    • 2006
  • Secalonic acid D(SAD) was isolated as the major secondary metabolite of the marine lichen-derived fungus Gliocladium sp. T31. Its structure was established on the basic of physicochemical and spectroscopic data. This is the first report on the isolation of SAD from this fungus, as well as its inhibitory effect on K562 cell cycle and its cytotoxicity against several tumor cell lines in vitro.

A highly efficient computational discrimination among Streptococcal species of periodontitis patients using 16S rRNA amplicons

  • Al-Dabbagh, Nebras N.;Hashim, Hayder O.;Al-Shuhaib, Mohammed Baqur S.
    • 미생물학회지
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    • 제55권1호
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    • pp.1-8
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    • 2019
  • Due to the major role played by several species of Streptococcus in the etiology of periodontitis, it is important to assess the pattern of Streptococcus pathogenic pathways within the infected subgingival pockets using a bacterial specific 16S rRNA fragment. From the total of 50 patients with periodontitis included in the study, only 23 Streptococcal isolates were considered for further analyses, in which their 16S rRNA fragments were amplified and sequenced. Then, a comprehensive phylogenetic tree was constructed and in silico prediction was performed for the observed Streptococcal species. The phylogenetic analysis of the subgingival Streptococcal species revealed a high discrimination power of the 16S rRNA fragment to accurately identify three groups of Streptococcus on the species level, including S. salivarius (14 isolates), S. anginosus (5 isolates), and S. gordonii (4 isolates). The employment of state-of-art in silico tools indicated that each Streptococcal species group was characterized with particular transcription factors that bound exclusively with a different 16S rRNA-based secondary structure. In conclusion, the observed data of the present study provided in-depth insights into the mechanism of each Streptococcal species in its pathogenesis, which differ in each observed group, according to the differences in the 16S rRNA secondary structure it takes, and the consequent binding with its corresponding transcription factors. This study paves the way for further interventions of the in silico prediction, with the main conventional in vitro microbiota identification to present an interesting insight in terms of the gene expression pattern and the signaling pathway that each pathogenic species follows in the infected subgingival site.

중학교 기하 교재의 '원론' 교육적 고찰 (A Study on Teaching of the Elements of Geometry in Secondary School)

  • 우정호;권석일
    • 대한수학교육학회지:수학교육학연구
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    • 제16권1호
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    • pp.1-23
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    • 2006
  • 본 논문은 중학교 평면 논증기하를 원론 교육적 입장에서 분석 고찰한 것이다. 이를 위하여 먼저 'Euclid 원론'에 따른 고전적 원론 교육을 목적, 내용, 방법의 측면에서 분석하고 그 역사를 개관하였다. 이어 고전적 원론 교육에 대한 비판적 논의를 고찰하고 Clairaut의 '기하학 원론'과 Branford의 역사-발생적 기하 교육론을 중심으로 역사-발생적 기하 원론 교육을 목적, 내용, 방법의 측면에서 분석하였다. 그리고 이러한 분석과 근세 이후 기하교과서의 변천과정에 비추어 현재의 중학교 논증기하 교재의 기본가정을 분석하고, 그 내용 및 체제를 가설적 작도, 정리의 제시순서, 증명진술 방법, 정의제시 방법, 연습문제로 나누어 분석하였다. 마지막으로 이러한 논의를 바탕으로, 현 중학교 기하교재의 기본적 관점을 탐색하고 두 원론의 상보적 통합 방안을 모색하였다.

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PTA법에 의한 스텔라이트 12 합금 육성층의 조직과 경도에 미치는 전류와 예열온도의 영향 (The Effect of Current and Preheat Temperature on Structure and Hardness of Stellite 12 Alloy Overlayer by PTA Process)

  • 정병호;김무길;김규덕;김민영;이성열
    • 열처리공학회지
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    • 제13권4호
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    • pp.246-252
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    • 2000
  • Stellite 12 alloy-powder was overlaid on 410 stainless steel valve seat using plasma transferred arc(PTA) process. Variation of characteristic of microstructure and hardness of deposit with current(90~150 A) and preheat temperature(R.T.~$400^{\circ}C$) was investigated. Important conclusion obtained are as follows; All welding conditions used produced a sound deposit layer with no defect in single pass welding. The maximum deposit had 4.0~4.8 mm in thickness and its bead width was increased with increase of current and preheat temperature. The deposit showed hypoeutectic microstruture, which was consisting of primary cobalt dendrite and networked $M_7C_3$ type eutectic carbides. The amount of eutectic carbides was decreased and its dendritic secondary arm spacing was increased with increase of current. Hardness of the deposit was decreased with increase of current. Preheat temperature up to $400^{\circ}C$, however, showed little influence on the hardness and microstructure. The hardness was also influenced by diluted Fe content near the interface in addition to microstructure and dendritic secondary arm spacing. Hot hardness at $500^{\circ}C$ showed higher than 300 HV.

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