• 한국의료질향상학회지
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• 제21권1호
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• pp.66-76
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• 2015

Objectives: The purpose of this study is to develop a case review form for detecting adverse events through a medical records review in hospitalized patients in South Korea. Methods: To develop the case review form, several literatures were reviewed, first. Through the clinical expert meeting, screening criteria were selected and case review form was developed. Result: The Korean version of case review form consisted of the review form-1 for adverse event screening and form-2 for adverse event identification. The applied methodology for the case review form is determined according to the previous studies. For example, the method used in the first stage review is nurse review. Furthermore, the National Coordinating Council for Medication Errors Reporting and Prevention index is used to categorize disability, and a scale of 1 to 6 was used in the causation scores and preventability scores, respectively. Through the clinical expert meeting, a total of 41 screening criteria were selected. Conclusion: The Korean specific case review form was developed for detecting adverse events in hospitalized patients. The results from this study can be used in a large-scale study regarding the nationwide incidence of adverse events.

• Weed & Turfgrass Science
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• 제4권2호
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• pp.118-123
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• 2015

메타게놈(metagenome)은 연구실에서 배양이 불가능한 미생물을 포함한 자연계에 존재하는 미생물의 유전자를 직접 연구하는 학문분야이다. 지구상 거의 모든 자연, 인공 환경에서 살고 있는 미생물 DNA를 분리 정제하는 것이 가능하며, 재조합 DNA 기술 등을 이용하여 배양 가능한 숙주미생물에 메타지놈을 클로닝함으로서 메타지놈 라이브러리를 제작할 수 있다. 최근 메타게노믹스를 통하여 자연계에 존재하고 있는 대다수의 미생물들이 실험실에서 배양되지 않았던 이유를 구명할 수 있게 되었고, 그들이 가지고 있는 생태학적인 의미와 역할에 대한 이해와 더불어 점차 그 응용 분야와 범위도 확대되고 있다. 이와 같은 메타게놈의 응용 분야 확대의 한 방안으로 본 연구에서는 새로운 제초활성 물질 및 제초활성 물질 생산에 필요한 유전자를 확보하기 위해 메타게놈 라이브러리를 대상으로 오이 떡잎절편 검정, 미세조류 생장저해 검정, 종자발아 저해 검정의 HTS (high throughput screening) 시스템을 구축하였고, 구축된 시스템으로부터 선발된 최종 단일 클론인 9-G1과 9-G12의 바랭이에 대한 in vivo assay를 통해 본 연구에서 개발한 HTS 시스템의 유효성을 확인 하였다. 후속연구로서 활성단일클론이 만들어내는 제초활성물질의 동정 및 제초활성물질을 합성하는 유전자군에 대한 연구를 수행 중에 있다.

• 생명과학회지
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• 제26권5호
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• pp.571-583
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• 2016

바이오제닉 아민은 주로 발효식품에 존재하는 미생물에 의해 발생하며, 종종 사람의 체내에서 심각한 독성을 나타내기도 한다. 따라서 바이오제닉 아민 비생성 균주의 선별을 위하여 순창군에서 제조된 전통 장류 200여 종으로부터 620종의 다양한 미생물을 분리하였고, 이중에서 다양한 생리활성 분석 및 바이오제닉 아민 생성여부를 조사하여 최종적으로 바이오제닉 아민 비생성 균주로써 Bacillus subtilis SCJ1을 선별하였다. 최종 선별한 SCJ1의 생화학적 특성과 16S rRNA 염기서열 분석을 통하여 Bacillus subtilis SCJ1로 명명하였다. Bacillus subtilis SCJ1의 산업적 적용이 가능한 최적배지 조성을 확립하기 위해 먼저 SCJ1균체 성장을 조사하였으며, 이를 기반으로 통계학적 방법인 반응표면 분석법을 사용하여 최적화를 수행하였다. 실험계획법은 배지 조성 성분의 선별을 위해 Plackett-Burman design을 사용하여 모델을 설정하였다. 실험 모델을 통하여 선별된 배지 성분으로는 molasses, yeast extract, peptone으로 예측되었으며, 배지조성에 대한 최적 농도를 설정하기 위해 중심합성계획법을 사용하여 최적화를 수행하였다. 최종적으로 예측된 배지 성분의 농도는 molasses 27.5 g/l, yeast extract 7.5 g/l, peptone 17.5 g/l로 예측되었고, 이때의 최대 균체량은 2.03704 g/l로 예측되어 모델의 검증실험을 통한 예측 모델과 실제 결과가 동일함을 확인하였다. 또한, 기본 배지와 균체량을 비교하였을 때 1.32 g/l에서 1.9722 g/l로 약 49.4% 증가하였고, 효소활성과 항균활성 또한 증가함을 확인하였다.

• Corrosion Science and Technology
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• 제8권3호
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• pp.119-125
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• 2009

Corrosion assessment has a number of uses but the use considered here is as a precursor to Risk-Based Inspection (RBI) planning. Systematic methods consisting of technical modules of RBI program were used to assess the effect of specific corrosion mechanism on the probability of failure in equipments of petrochemical plants. Especially in part of the damage and corrosion assessment, screening step involved evaluating the combinations of process conditions and construction materials for each equipment item in order to determine which damage mechanisms are potentially active. For general internal corrosion, either API 510 or API 570 was applied as the damage rate in the calculation to determine the remaining life and inspection frequency. In some cases, a measured rate of corrosion may not be available. The technical modules of RBI program employ default values for corrosion, typically derived from published data or from experience with similar processes, for use until inspection results are available. This paper describes the case study of corrosion and damage assessment by using RBI methodology in petrochemical plant. Specifically, this paper reports the methodology and the results of its application to the petrochemical units using the $KGS-RBI^{TM}$ program, developed by the Korea Gas Safety Corporation to suit Korean situation in conformity with API 581 Codes.

• 한국축산식품학회지
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• 제39권1호
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• pp.114-120
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• 2019

Response surface methodology (RSM) is a useful set of statistical techniques for modeling and optimizing responses in research studies of food science. As a design for a response surface experiment, a central composite design (CCD) with multiple runs at the center point is frequently used. However, sometimes there exist situations where some among the responses at the center point are outliers and these outliers are overlooked. Since the responses from center runs are those from the same experimental conditions, there should be no outliers at the center point. Outliers at the center point ruin statistical analysis. Thus, the responses at the center point need to be looked at, and if outliers are observed, they have to be examined. If the reasons for the outliers are not errors in measuring or typing, such outliers need to be deleted. If the outliers are due to such errors, they have to be corrected. Through a re-analysis of a dataset published in the Korean Journal for Food Science of Animal Resources, we have shown that outlier elimination resulted in the increase of the maximum possible R-square that the modeling of the data can obtain, which enables us to improve the quality of response surface analysis.

• Child Health Nursing Research
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• 제28권2호
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• pp.91-102
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• 2022

Purpose: This study systematically analyzed cases in South Korea wherein nurses were prosecuted for involuntary manslaughter or injury due to professional negligence in pediatric care. Methods: We analyzed the precedents using the methodology of Hall and Wright (2008) and Austin (2010). Of the 618 cases retrieved from the Supreme Court Decisions Retrieval System in South Korea, we selected the 12 cases in which children were the victims and nurses were the defendants, using a case screening methodology. Results: The most frequent penalty was a fine, and newborns were the most frequent victims. The distribution of cases according to Austin's violation categories was: improper administration of medications (n=5), failure to monitor for and report deterioration (n=4), ineffective communication (n=4), failure to delegate responsibly (n=4), failure to know and follow facility policies and procedures (n=1), and improper use of equipment (n=1). Conclusion: To ensure the safety of children, nurses are required to teach and practice a high standard of care. Nursing education programs must improve nurses' awareness of their legal obligations. Nursing organizations and leaders should also work towards enacting effective nursing laws and ensuring that nurses are aware of their legal rights and responsibilities.

• 산경연구논집
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• 제14권10호
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• pp.1-9
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• 2023

Purpose: In an increasingly globalized world, foreign direct investment (FDI) plays an essential role in the economic improvement of countries. This study aims to delve into the topic of overseas direct investment (FDI) and offer a complete analysis of its strategic advantages and disadvantages. By thoroughly examining the present literature, this study aims to discover and explore the diverse advantages and drawbacks. Research design, data and methodology: The information analysis system systematically and rigorously examined the selected studies. The evaluation will follow a thematic technique in which conventional subject matters and styles associated with FDI's strategic benefits and downsides can be recognized and synthesized. Data extraction contained relevant facts from the chosen research, along with the study objectives. Results: This study provides the findings of the, which explores the strategic advantages and disadvantages of foreign direct investments (FDI) primarily based on the evaluation of previous research. A comprehensive review of the identified benefits and drawbacks highlights their implications for businesses engaged in FDI. Conclusions: In sum, the findings offer valuable insights for practitioners, guiding their decision-making methods in the international commercial enterprise landscape. Organizations can function for fulfillment and sustainable development in the global marketplace by leveraging the advantages and effectively managing demanding situations.

• 유통과학연구
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• 제22권6호
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• pp.107-114
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• 2024

Purpose: The current research investigates historical and future trends of tourist distribution channels in the tourism services business. The research examines historical patterns, current shifts, and new technologies in electricity distribution to offer insight into the distribution dynamics and advice for companies and regulators. Research design, data and methodology: The research in this case specifically employed the PRISMA approach when it comes to the data collection and research methodology. (PRISMA). The process is specifically made up of four steps, such as (1) Identification of Relevant Studies, (2) Screening and Selection Procedures, (3) Data Synthesis and Analysis, and (4) Reporting of Findings. Results: The fast-changing technology offers all opportunities to innovate the sector of tourism services. These upcoming technologies are not just reconstructing the way customers interact and operate but they are also creating room for development. Besides "the utilization of new technologies such as artificial intelligence, augmented reality, virtual reality, and blockchain, the current state of tourism distribution channels also implies some other possible consequences. Conclusions: These research results show that we should not be reluctant about adopting new technologies, we should expand direct booking systems, promote eco-friendly tourism, and use data analytics in order to provide personalized experiences.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.965-974
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• 2017

The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, $55.2{\pm}0.3^{\circ}C$, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, $52.5{\pm}0.3^{\circ}C$, and 13.54 nm for the scFv expressed by E. coli. This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.

• 한국대기환경학회지
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• 제15권2호
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• pp.139-150
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• 1999

The ozone concentrations measured at the National Air Monitoring Stations between 1990 and 1995 were reviewed to detect any anomalies in the measurements. By screening the cases, in which variation of the ozone concentration from the previous measured value is greater than 75ppb, 125 station-days were identified as the test cases for the anomaly test. Historical and parallel consistencies of the measured concentrations were examined by plotting data for each test case. The detected anomalies can be classified into four categories; single outliers, anomalous variations during the startup period, baseline rises, and fluctuations in th diurnal variations. Anomalies were detected in as many as 80 cases among 125 test cases. Because of these anomalies, the number of hours exceeding 100ppb in the areas other than the Greater Seoul Area(GSA) could decrease from 157 to 107. Further studies for developing the methodology for eliminating the abnormal monitoring data are warranted for the data from the National Air Monitoring Stations are official to the both inside and outside of the country.