• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.20-20
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• 2015

Endophytic fungi are microorganisms inhabiting living plant tissues without causing apparent harm to the host. They are drawing increasing attention due to their ability to produce various bioactive compounds as well as their effects on host growth and resistance to biotic and abiotic stresses. As a first step to assess biodiversity of plant associated fungi in Korea and the following evaluation on diverse biological activities, we are collecting endophytic fungi from plant in wild followed by systematic long-term storage in liquid nitrogen. Molecular identification using ITS sequences was also incorporated for pure culture by hyphal tip isolation. As of April 2015, about 1,400 fungal strains had been isolated from about 170 plant taxa. Fungal isolates belonging to Pleosporales, Diaporthales, Glomerellales, Hypocreales, and Xylariales were the most abundant. These collections are being used for several complementary researches, including screening of isolates with novel bioactive compounds or conferring drought stress resistance, phylogenetic and genomic study. Genome sequencing was performed for 3 isolates, one Xylaria sp. strain JS573 producing griseofulvin, an antifungal compound, and two Fusarium spp. strains JS626 and JS1030, which are assumed to be new species found in Korea. More detailed analysis on these genomes will be presented. These collections and genome informations will serve as invaluable resources for identifying novel bioactive materials in addition to expand our knowledge on fungal biodiversity.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.252-255
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• 2012

게임의 멀티미디어 요소인 그래픽과 사운드 등은 '몰입'이라는 게임 플레이의 중요한 역할을 한다. 따라서 게임의 품질은 멀티미디어 요소의 품질과 밀접한 관계가 있다고 할 수 있다. 본 연구에서는 콘텐츠 분야의 각종 공모전 심사 기준과 선행 연구를 바탕으로 게임의 멀티미디어 요소에 대한 품질 평가 영역과 영역별 세부 하위 요소들을 추출하였다. 추출된 평가 영역과 하위 요소에 대한 필요성을 검증하기 위해 게임 이용자들을 대상으로 설문을 실시할 것이다. 연구 결과는 향후 게임 제작에 있어서 중요한 참고 자료가 될 것으로 본다.

• Journal of Chest Surgery
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• 제27권9호
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• pp.723-731
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• 1994

We have modified the isolated perfused working rabbit lung model [IPWL] by perfusing the isolated lung with a hollow fiber membrane deoxygenator.For assessment the stored lung was ventilated with FIO2 0.4 and perfused with 37$^{\circ}$C deoxygenated circulating blood at a rate 5ml/kg/min for several hours until lung failure.We chose to compare our developing solution which contained low potassium and pentastarch with the modified Euro-Collins solution .Experiments were divided into four groups[n=6] based on the type of flushing preservation solution and preservation time.The flushed lungs were then preserved into same solution at 8~10$^{\circ}$C with 100% O2 inflated condition for 1 or 20 hours.These following results were obtained.The IPWL model requires only one animal per experiment and allows for the continuous assessment of aerodynamic performance. This should therefore be used as screening test in lung preservation.One hour preservation groups, there were no significant difference in recovery rates of PaO2, PAP and Paw. Survival time in the one hour preservation groups were very significant long in the Group II[LPPS, p<0.01]. Twenty hours preservation groups, there were no significant difference in the recovery rates of PAP and Paw between Group III[m-ECS] and Group IV[NS], but PaO2 was significantly worse at onset of reperfusion in Group III when compared with Group IV [p<0.05]. And also survival time in the 20 hours preservation groups were significant long in the Group IV [p<0.05].

• Journal of Microbiology and Biotechnology
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• 제13권4호
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• pp.495-500
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• 2003

Tight junctions (TJ) between adjacent Sertoli cells in testis are important for the formation of the blood testis barrier (BTB). In an effort to verify the reproductive health risk of endocrine-active chemicals (EACs), changes in the transepithelial electrical resistance (TER) and the expression of TJ genes were examined by co-planar polychlorinated biphenyl (PCB) treatment in cultured mouse Sertoli cells. Although the increase in TER of Sertoli cells was accelerated by 10 nM co-planar PCB, it was downregulated by 100 nM co-planar PCB. The expression of claudin-1 was downregulated by co-planar PCB in a concentration-dependent manner. On the contrary, the expression of claudin-1 was increased in the Sertoli cells by 10 nM co-planar PCB treatment. These results suggest that the structure and function of TJ may be targeted by co-planar PCB in Sertoli cells. Assessment of the structure and function of TJ in Sertoli cells might be useful for screening the reproductive health risk of EACs.

• Journal of Plant Biotechnology
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• 제35권1호
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• pp.23-29
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• 2008

생명공학기술을 이용한 유전변형작물의 창출은 세계 인구의 증가와 식량 수요의 증가에 대응하는 주요한 기술로 인정받고 있다. 이미 유전변형작물의 상업화에 성공한 선진국의 선례에 바탕하여 우리나라도 지난 20년 동안 유전변형 작물의 개발을 지속하여 20여 종의 작물에서 60여 건 이상의 유전변형작물을 개발하였다. 본 고에서는 우리나라의 유전변형작물 개발과 위해성 평가 현황을 간단히 살펴보면서, 우리나라에서 개발된 유전변형작물의 상업화가 현재까지 이루어지지 못하는 죽음의 계곡이 어디에 있는지를 제시하고자 하였다. 저자는 지난 5년 동안 15건의 국내개발 유전변형작물의 위해성 평가를 수행한 경험에 바탕하여, 우량 사상의 선발의 부재가 상업화의 죽음의 계곡임을 제시하였다.

• 한국토양비료학회지
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• 제48권2호
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• pp.100-104
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• 2015

Adverse effect of veterinary antibiotics (VAs) released into environment has been issued recently and concerns about analysis and management for VAs in the environment were increased. Main objective of this research was to calculate predicted environmental concentration (PEC) of the VAs in soil based on avaiable statistical data and result of previous study such as consumption rate and physiological properties of VAs. Total of 5 VAs, Chlortetracycline (CTC), Oxytetracycline (OTC), Sulfadimethoxine (SDX), Sulfamethazine (SMT), and Tylosin (TYL) were examined. Result showed that calculated PEC value in manure and soil was ordered as SMT > TYL > SDX > CTC > OTC. Range of calculated value for manure and soil was 0.50-67.04 and $0.48-64.45mg\;kg^{-1}$ respectively. Comparing to measured concentration of VAs in manure and soil, lower concentration of VAs in manure and soil was evaluated due to fate and degradation of VAs in manure and soil. Overall, evaluated simple modeling for calculating PEC of VAs in manure and soil can be adapted for preliminary screening purpose in environmental risk assessment and more refined modeling is necessary to examine detailed assessment of VAs in manure and soil.

• Child Health Nursing Research
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• 제12권2호
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• pp.260-267
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• 2006

Purpose: This pilot study was to develop Korean version of CDI(The Child Development Inventory) and assess the validation and reliability on K-CDI. Method: The K-CDI is assessed by parent-report method that has designed to screen children developmental functioning on 12 months to 6.5 years of age /or older children who are judged to be functioning in the one to six-year range. The inventory assesses areas of child development in the social, self-help, motor, language, letter and number skills. also, it includes various symptoms and behavior problems that young children may have. Participants were 130 children and their mothers who has lived in Seoul or Gyounggi-Do. Data was analyzed on the ratio of item response, Pearson's correlation, and Cronbach alpha. Results: 270 items with 8 sub-developmental realms were confirmed the reliabilities and validity on Korean children. Total 300 items were selected for restandardization. There was a significant correlation between the score of Social Maturity Scale and K-CDI. The corrlation coefficient alpha was .98. Conclusion: This study indicated that CDI is applicable in the clinical and early childhood educational setting for developmental assessment in Korea.

• 대한전기학회논문지:전력기술부문A
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• 제51권6호
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• pp.262-268
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• 2002

Preventive control has to solve two important problems. The first is fast and accurate severity assessment of instability originated from the occurrence of a dangerous contingency. The second is to choose an action able to stabilize it. In this paper we assess contingencies in power systems using PASF(Power Angle Shape Filtering) and control power systems by a generation rescheduling. The control action stabilize the whole set of harmful contingencies simultaneously. Note that conventional time-domain transient stability methods can hardly tackle preventive control. So, we study the preventive control using off-line method. The proposed method is applied to prevent and to correct loss of synchronism of all the generators in a operating systems data.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 춘계학술대회 논문집
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• pp.195-200
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• 2008

The accuracy of classifying pixels in HIRIS images is usually degraded by noisy bands since noisy bands may deform the typical shape of spectral reflectance. Proposed in this paper is a statistical method for noisy band removal which mainly makes use of the correlation coefficients between bands. Considering each band as a random variable, the correlation coefficient measures the strength and direction of a linear relationship between two random variables. While the correlation between two signal bands is high, existence of a noisy band will produce a low correlation due to ill-correlativeness and undirectedness. The application of the correlation coefficient as a measure for detecting noisy bands is under a two-pass screening scheme. This method is independent of the prior knowledge of the sensor or the cause resulted in the noise. The classification in this experiment uses the unsupervised k-nearest neighbor algorithm in accordance with the well-accepted Euclidean distance measure and the spectral angle mapper measure. This paper also proposes a hierarchical combination of these measures for spectral matching. Finally, a separability assessment based on the between-class and within-class scatter matrices is followed to evaluate the performance.

• Toxicological Research
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• 제29권4호
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• pp.221-227
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• 2013

Embryonic stem (ES) cells have potential for use in evaluation of developmental toxicity because they are generated in large numbers and differentiate into three germ layers following formation of embryoid bodies (EBs). In earlier study, embryonic stem cell test (EST) was established for assessment of the embryotoxic potential of compounds. Using EBs indicating the onset of differentiation of mouse ES cells, many toxicologists have refined the developmental toxicity of a variety of compounds. However, due to some limitation of the EST method resulting from species-specific differences between humans and mouse, it is an incomplete approach. In this regard, we examined the effects of several developmental toxic chemicals on formation of EBs using human ES cells. Although human ES cells are fastidious in culture and differentiation, we concluded that the relevancy of our experimental method is more accurate than that of EST using mouse ES cells. These types of studies could extend our understanding of how human ES cells could be used for monitoring developmental toxicity and its relevance in relation to its differentiation progress. In addition, this concept will be used as a model system for screening for developmental toxicity of various chemicals. This article might update new information about the usage of embryonic stem cells in the context of their possible ability in the toxicological fields.