• Asian Pacific Journal of Cancer Prevention
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• v.16 no.11
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• pp.4641-4645
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• 2015

The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to $10-1000{\mu}g/ml$ concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at $1000{\mu}g/ml$ each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.

• Advances in Traditional Medicine
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• v.4 no.3
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• pp.187-195
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• 2004

A number of plants from different geographical origins have been shown to possess antioxidant activity. Some of them have been developed as natural antioxidant formulations for food, cosmetic and other applications. Bangladeshi flora is a rich source of a range of plant species, many of which are medicinal plants, and have been used in the preparations of the Unani and Ayurvedic traditional medicines. There are no, or just a few, reports on any systematic screening of the extracts of Bangladeshi plants for free radical scavenging activity using DPPH assay available to date. As part of our on-going search for biological activity in Bangladeshi plants, Kadam (Anthocephalus chinensis), Goran (Ceriaps decandra), Swarnalata (Cuscuta reflexa), Gab (Diospyros peregrina), Sundari (Heritiera fomes), Dhundul (Xylocarpus granatum) and Possur (Xylocarpus mekongensis) have been selected for the assessment of their free radical scavenging activity, and studies on the contents of alkaloids, anthraqunones, flavonoids and tannins in these extracts. Most of these species have been used in traditional medicine in Bangladesh and other countries for the treatment of various illnesses ranging from common cold to cancer. All extracts, except the methanol extract of Cuscuta reflexa, displayed significant free radical scavenging activity in the DPPH assay $(RC_{50}$ values within the range of $2.75\;{\times}\;10^{-2}\;to\;4.7\;{\times}\;10^{-3}\;mg/mL)$. Among these extracts, the methanol extract of Xylocarpus granatum exhibited the most potent activity $(4.7\;{\times}\;10^{-3}\;mg/mL)$ and that of Cuscuta reflexa had the least activity $(1.64\;{\times}\;10^{-1}\;mg/mL)$. While none of these plants showed positive tests with Dragendorff's reagent, presence of low to moderate amounts of phenolic compounds, e.g. anthraquinones, flavonoids and tannins was evident in all of these plants, except for the methanolic extracts of C. reflexa and the barks of D. peregrina, which did not display any evidence for the presence of flavonoids and anthraquinones, respectively.

• Molecular & Cellular Toxicology
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• v.2 no.2
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• pp.114-119
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• 2006

Benzo[a]pyrene is a representative ecotoxicant in marine environment and a model compound of polycyclic aromatic hydrocarbons, which has an ability to bioaccumulate in aquatic organisms. This study aimed to identify molecular biomarkers suitable for assessing environmental pollution using a microarray technique. We examined the effects of benzo[a]pyrene on gene expressions in the rockfish, Sebastes schlegeli. We constructed the subtractive cDNA library with hepatic RNA from benzo[a]pyrene-exposed and non-exposed control fish. From the library 10,000 candidate clones were selected randomly and cDNA microarray was constructed. We determined benzo[a]pyrene-responsive genes using a high-density microarray. Statistical analysis showed that approximately 400 genes are significantly induced or reduced by benzo[a]pyrene treatment ($2\;{\mu}m$). Especially gene expression changes of 4 candidate clones among the up- or down-regulated genes were investigated in 6, 12 and 24 hr BaP-exposed fish groups. Many methods have been developed to monitor marine environmental status, which depend on quantifying the levels of the toxic components in polluted seawater or on ecological accessing, such as species diversity or richness. However, those methods could not provide information on physiological or genetic changes induced by such environmental stresses. Comparing with the conventional methods, these data will propose that benzo[a]pyrene-responsive genes can be useful for biological risk assessment of polycyclic aromatic hydrocarbons on marine organism at molecular level.

• Journal of Mechanical Science and Technology
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• v.19 no.2
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• pp.634-648
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• 2005

Performed here is a comparative assessment study for the probabilistic fracture mechanics approach of the pressurized thermal shock of the reactor pressure vessel. A round robin consisting of one prerequisite deterministic study and five cases for probabilistic approaches is proposed, and all organizations interested are invited. The problems are solved by the participants and their results are compared to issue some recommendation of best practices and to assure an understanding of the key parameters in this type of approach, like transient description and frequency, material properties, defect type and distribution, fracture mechanics methodology etc., which will be useful in the justification through a probabilistic approach for the case of a plant over-passing the screening criteria. Six participants from 3 organizations responded to the problem and their results are compiled and analyzed in this study.

• Journal of Preventive Medicine and Public Health
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• v.47 no.2
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• pp.84-93
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• 2014

Objectives: The incidence and survival rate of colorectal cancer in Korea are increasing because of improved screening, treatment technologies, and lifestyle changes. In this aging population, increases in economic cost result. This study was conducted to estimate the economic burden of colorectal cancer utilizing claims data from the Health Insurance Review and Assessment Service. Methods: Economic burdens of colorectal cancer were estimated using prevalence data and patients were defined as those who received ambulatory treatment from medical institutions or who had been hospitalized due to colorectal cancer under the International Classification of Disease 10th revision codes from C18-C21. The economic burdens of colorectal cancer were calculated as direct costs and indirect costs. Results: The prevalence rate (per 100 000 people) of those who were treated for colorectal cancer during 2010 was 165.48. The economic burdens of colorectal cancer in 2010 were 3 trillion and 100 billion Korean won (KRW), respectively. Direct costs included 1 trillion and 960 billion KRW (62.85%), respectively and indirect costs were 1 trillion and 160 billion (37.15%), respectively. Conclusions: Colorectal cancer has a large economic burden. Efforts should be made to reduce the economic burden of the disease through primary and secondary prevention.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6413-6416
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• 2015

Colorectal cancer incidence and mortality in Kazakhstan are relatively high but exact statistics have hitherto been lacking and trends over time are unclear. The present study was therefore undertaken to retrospectively assess data for East Kazakhstan, accessed from the central registration office, for the period 2004-2013. Approximate age standardized data for incidence and mortality were generated and compared across age groups, gender and year. It was determined that during the studied period 3,417 new cases of colorectal cancer were registered and 2,259 died of this pathology. Average cancer cancer incidence and mortality over the ten years were $24.1/10^5$ and $15.9/10^5$ respectively, and the overall ratio of mortality/incidence (M/I) was 0.69:1 (range 0.58-0.73). Both incidence and mortality tended to remain constant in both males and females. The male to female ratios also did not significantly vary over time but a trend for improvement of the mortality to incidence ratio was observed, especially for rectum. Whether this might be related to screening remains unclear. These preliminary data indicate that whereas colorectal cancer continues to be important, change in environmental factors are not having a great impact on incidence in East Kazakhstan.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.15
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• pp.6795-6798
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• 2015

Breast cancer incidence and mortality in Kazakhstan are considered to be increasing but exact statistics have hitherto been lacking. The present study was therefore undertaken to retrospectively assess data for the whole country, accessed from the central registration office, for the period 1999-2013. Age standardized data for incidence and mortality were generated and compared across age groups. It was determined that during the studied period 45,891 new cases of breast cancer were registered and 20,122 women died of this pathology. Average breast cancer incidence and mortality were $37.9{\pm}1.10/10^5$ and $16.7{\pm}0.20/10^5$ respectively, and the overall ratio of mortality/incidence (M/I) was 0.44. Incidence tended to increase (T = + 2.3%), and mortality to decrease (T of =-0.3%). Peaks of incidence and mortality were noted in those aged 60-74 years and 75-84, respectively. Particularly high incidences were established in large cities of Kazakhstan, Astana ($46.8{\pm}1.80/10^5$) and Almaty ($49.7{\pm}1.30/10^5$), and high mortality was observed in the Pavlodar region ($17.9{\pm}0.60/10^5$) and Almaty city ($20.1{\pm}0.40/10^5$). Considerable variation in the mortality/incidence ratio was noted, suggesting the need for more stress on access to screening and clinical care in some regions of the country.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.7
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• pp.4161-4165
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• 2013

Objective: To investigate discriminating protein patterns and potential biomarkers in serum samples between pre/postoperative pancreatic cancer patients and healthy controls. Methods: 23 serum samples from PC patients (12 preoperative and 11 postoperative) and 76 from healthy controls were analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique combined with magnetic beads-based weak cation-exchange chromatography (MB-WCX). ClinProTools software selected several markers that made a distinction between pancreatic cancer patients and healthy controls. Results: 49 m/z distinctive peaks were found among the three groups, of which 33 significant peaks with a P < 0.001 were detected. Two proteins could distinguish the preoperative pancreatic cancer patients from the healthy controls. About 15 proteins may be potential biomarkers in assessment of pancreatic cancer resection. Conclusion: MB-MALDI-TOF-MS method could generate serum peptidome profiles of pancreatic cancer and provide a new approach to identify potential biomarkers for diagnosis and prognosis of this malignancy.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.19 no.3
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• pp.156-161
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• 2008

Objectives: The purpose of this study was to investigate the prevalence of sleep problems in Korean elementary school children and the differences in sleep problems elated to their school grade and gender. Methods : One public elementary school in Dae-gu, Korea as randomly selected. And, parents who agreed to participate this study were asked to complete a modified version of Tucson Children's Assessment of Sleep Apnea screening questionnaire (TuCASA). Results: The most common sleep problem was 'falling asleep in vehicle (44.2%)' and the prevalence rate of this problem differed by school grade. The prevalence rate of falling asleep while watching TV before 8 P.M. (8.0%), daytime fatigue (21.9%) and nocturnal enuresis during the past 6 months (5.3%) were significantly differed by school grade. The prevalence rate of falling asleep while doing home work, falling sleep during a lesson, snoring and bruxism were 12.6%, 1.0%, 26.7% and 13.2% respectively. Although these rates did not show any differences between grades, snoring as more in boys and daytime fatigue was more in girls. Conclusion: This study demonstrates that many elementary school children may have various sleep problems. Lower grade elementary schoolers had sleep problems which related to euro-developmental factors, whereas higher grade elementary schoolers had sleep problems related to sleep deprivation and stress. Finally, more female schooler showed signs of fatigue or sleepiness than male schoolers.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.49 no.2
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• pp.38-45
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• 2012

If an infant with congenital hearing loss is diagnosed in good time and get proper treatment as soon as possible, treatment effect could be maximized and the social costs could be considerably reduced. For these reasons, the medical equipment to screen hearing impairment objectively is needed. The ABR(auditory brainstem response) is typical diagnostic tools for this purpose but there are drawbacks in sense that it does not have frequency specificity and shows hearing information of usually high frequency band. The ASSR(auditory steady-state response) is excellent in frequency specificity, but the rate of wrong diagnosis is slightly high. In this study, we proposed the system which can measure both the ABR and the ASSR, and can show the objective and quantitative indices(Fsp and F-test). It was designed to allow various tests without hardware modification by minimizing hardware components and by increasing software roles. The objective assessment of the developed system was evaluated by experiments with 10 normal persons.