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http://dx.doi.org/10.7314/APJCP.2015.16.11.4641

Cytotoxicity Assessment of Six Different Extracts of Abelia triflora leaves on A-549 Human Lung Adenocarcinoma Cells  

Al-Taweel, Areej Mohammad (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Perveen, Shagufta (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Fawzy, Ghada Ahmed (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Ibrahim, Taghreed Abdou (Department of Pharmacognosy, College of Pharmacy, King Saud University)
Khan, Afsar (Department of Chemistry, COMSATS Institute of Information Technology)
Mehmood, Rashad (Department of Conservation Studies, Hazara University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.11, 2015 , pp. 4641-4645 More about this Journal
Abstract
The present investigation was designed to assess the anticancer activity of six different leaf extracts (ethyl acetate, methanol, chloroform, petroleum ether, n-butanol, and water soluble) of Abelia triflora on A-549 human lung adenocarcinoma epithelial cells. A-549 cells were exposed to $10-1000{\mu}g/ml$ concentrations of the leaf extracts of A. triflorafor 24 h and then percentage cell viability was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay. The results showed that leaf extracts of A. triflora significantly reduced the viability of A-549 cells in a concentration-dependent manner. Decrease was recorded as 31% with ethyl acetate, 36% with methanol, 46% with chloroform, 54% with petroleum ether, 62% with n-butanol, and 63% with water soluble extracts at $1000{\mu}g/ml$ each. Among the various plant extracts, ethyl acetate extract showed the highest decrease in the percentage cell viability, followed by methanol, chloroform, petroleum ether, n-butanol, and water soluble extracts. Our results demonstrated preliminary screening of anticancer activity of different soluble extracts of A. triflora extracts against A-549 cells, which can be further used for the development of a potential therapeutic anticancer agents.
Keywords
Abelia triflora; A-549 cell line; cytotoxicity; MTT Assay;
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