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Extraction of Liberated Reducing Sugars from Rapeseed Cake via Acid and Alkali Treatments (산 및 알칼리 처리에 의한 유채박의 유리당 추출)

  • Jeong, Han-Seob;Kim, Ho-Yong;Ahn, Sye-Hee;Oh, Sei-Chang;Yang, In;Choi, In-Gyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1575-1581
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    • 2011
  • Rapeseed cake, which is the organic waste remaining after rapeseed oil production, is readily available and considered an ecologically-friendly resource with very low cost and high dietary fiber content. This research was carried out for two reasons. First, it was done to analyze the liberated reducing sugar content of rapeseed cake. Second, it was done to investigate the effects on the sugar yield of the various concentrations of acidic and alkaline catalysts used for the hydrolysis of rapeseed cake and the concentrations of rapeseed cake in each catalyst. Several amounts of ground rapeseed cake, 0.5 g, 1 g, and 2 g, were put into 100 mL of catalysts such as sulfuric acid (0.5~2%), hydrochloric acid (0.5~2%), and sodium hydroxide (0.5~2%). Then they were hydrolyzed for 5 min at 121$^{\circ}C$. After hydrolysis, HPLC equipped with an RI detector was used to analyze liberated reducing sugars such as sucrose, glucose, galactose, fructose, and arabinose separated from rapeseed cake. The degradation rate of rapeseed cake was the highest in hydrochloric acid. As the catalyst concentrations used for hydrolysis of rapeseed cake increased, the degradation rate of rapeseed cake also significantly increased. Total reducing sugar content was the highest in hydrochloric acid, and it increased with the increase of catalyst concentrations. However, as the amount of rapeseed cake increased, the total reducing sugar content decreased, exceptionally sucrose in the case of sodium hydroxide.

Analysis of 2009 Revised Chemistry I Textbooks Based on STEAM Aspect (STEAM 관점에서 2009 개정 화학 I 교과서 분석)

  • Bok, Juri;Jang, Nak Han
    • Journal of Science Education
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    • v.36 no.2
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    • pp.381-393
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    • 2012
  • This study was analyzed that what kind of elements for STEAM, except scientific commonsense, are contained in 2009 revised chemistry textbooks I for high school students. So first, elements of STEAM in textbooks were examined by following three sections; by publishing company, each unit and area of textbook. For reference, new sub-elements of STEAM were set because existing elements of STEAM is incongruent with current textbooks. As a result, most chemistry textbooks included elements of STEAM properly for inter-related learning with the other fields. Every textbook had its unique learning methods for utilizing elements of STEAM and they were unified as one way. Depending on textbooks, learning methods were little bit different from the others. Also, detailed elements of STEAM contained in textbooks were classified just 14 types. And they were even focused on a few elements according to sort of textbook. Thus, it seemed that there was a certain limitation of current education of STEAM in chemistry Field. By the unit, according to the curriculum, contained elements of STEAM were different. Almost all elements of STEAM were located in I section. Consequently, it is difficult to include elements of STEAM if mathematics or history were not existed in curriculum. Lastly, by the area, most of all elements of STEAM were included in reference section. Almost all elements of STEAM were focused on art and culture. Thus, STEAM was used for utilization about chemical knowledge in substance. Otherwise, convergence training for approach method was not enough in chemical knowledge.

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Changes of Microclimate Responses and Chlorophyll Content(SPAD) to Different Shading Materials on Korean Ginseng (Panax ginseng C. A. Meyer) Bed (인삼 재배 시 차광재료에 따른 미기상 반응과 엽록소의 함량 변화)

  • An, Young-Nam;Choung, Myoung-Gun;Kang, Kwang-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.4
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    • pp.397-406
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    • 2009
  • This study was carried out to investigate the microclimate (photosynthetically active radiation and temperature) response to different shading materials(shade plate, and polyethylene net) on Korean ginseng(Panax ginseng C.A. Meyer) bed. Photosynthetically active radiation(PAR) under polyethylene net was approximately 24 ~ 30% higher than that under shade plate on sunny day. Also, PAR was remarkably decreased from the front to the rear rows of ginseng beds. Temperature under polyethylene net was higher than that of shade plate. Internal temperature under polyethylene net was 1.9 ~ 3.1% higher than that under shade plate at the different rows of ginseng bed. Mean of total daily temperature under polyethylene net was higher at the rear rows than at the front rows, while that under shade plate was higher at the front rows than at the rear row of ginseng bed. Mean of total daily temperature on sunny days(April 15 and May 17) was 7.3% lower under shade plate than above outside but, polyethylene net was 0.7% high, while that on cloudy days(April 11 and May 15) was 1.6% higher under polyethylene net than above outside, but shade plate was 0.7% low. Also, overall mean of total daily temperature under polyethylene net was 7.0, 7.8, and 8.8 % on sunny day(April 15 and May 17), and 1.7, 1.6, and 3.5% on cloudy day(April 11 and May 15) higher than that under shade plate, respectively. The SPAD values of 6 years old ginseng at two point(front, center) in bed under shade plate showed the highest value, and the lowest under polyethylene net. The SPAD of 6 years old ginseng at rear in bed was not statistically significant under two shading materials.

Characterization of the Bovine FASN Gene Variation for Carcass and Beef Quality Traits in Hanwoo (소 FASN 유전자 변이의 연관불균형과 한우 도체형질에 미치는 영향)

  • Li, Song-Lan;Kim, Sang-Wook;Lee, Jung-Jae;Lee, Jun-Heon;Yoon, Du-Hak;Kim, Jong-Joo;Jeong, Young-Chul;Jeon, Soon-Hong;Choi, Jae-Won;Kim, Nae-Su;Kim, Kwan-Suk
    • Journal of Animal Science and Technology
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    • v.51 no.3
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    • pp.185-192
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    • 2009
  • Fatty acid synthase (FASN) is a multi-functional enzyme with a central role in the synthesis of long-chain fatty acid and has been considered as a positional candidate gene for BTA 19 quantitative trait loci (QTL) affecting milk-fat content and fatty acid composition. In this study, we sequenced the FASN gene in several cattle breeds including Hanwoo and imported beef cattle, and identified novel DNA polymorphisms and their linkage relationship in Hanwoo. We found a significant frequency difference of the FASN (AF285607) g.17924 A$\rightarrow$G polymorphism between Hanwoo (70%) and other breeds and this polymorphism has been known for an association with fatty acid composition in Angus. Furthermore, by direct DNA sequencing in 18 unrelated Hanwoo, we identified 27 SNPs including nine novel variations in the FASN gene. Among 27 SNPs identified in the FASN gene, four SNPs were further genotyped in 100 Hanwoo and 96 imported beef cattle, and analyzed for haplotype construction and association with beef quality traits. We performed haplotype block and linkage disequilibrium studies using four selected SNPs. Two different haplotype blocks (block A: g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$ A; block B: g.13125 C$\rightarrow$T and g.17924 G$\rightarrow$A) were constructed and the block A in particular had a very high r2 (0.936), which indicated a nearly complete linkage disequilibrium existed between the g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$A polymorphisms. A total of four major haplotypes (frequency > 0.05) were identified with the four polymorphisms including TATG (0.36), CGCG (0.31), CGTA (0.19) and TACG (0.06). Statistical association analysis revealed that the g.10568 C$\rightarrow$T and g.11280 G$\rightarrow$A polymorphisms in the FASN were significantly associated with meat color (P=0.004) and texture (P=0.0114). The g.13125 C$\rightarrow$T and g.17924 G$\rightarrow$A polymorphisms in the FASN were also significantly associated with back-fat thickness and quantity index (P=0.0179 and 0.0495, respectively). Our findings suggested that the FASN gene polymorphisms may be used for determining the (unsaturated) fatty acid contents and carcass trait in the Hanwoo beef.

The Study on the Salinity of Kimchi and Subjective Perception of Salinity in Pusan Area (부산지역의 김치 염도 및 김치 염도에 대한 인식도)

  • 문갑순;송영선;이치간;김성경;류복미;전영수
    • Korean journal of food and cookery science
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    • v.13 no.2
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    • pp.179-184
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    • 1997
  • To investigate the salinity of Kimchi and subjective perception of salinity on Kimchi in Pusan area, questionaire and sampling of Kimchis was performed twice during summer and winter, 1996 and 1997. Most of subjects attend in this survey were in their thirties and fourties, graduated from high school and their monthly income was in the range of one million to two million won. Above 90% of subjects live in Pusan and Kyung-nam area and most of them learned how to make Kimchi from their mother and believed that the salinity of home-made Kimchi is constant through the year. The survey on the subjective perception of salinity of summer Kimchi showed that salinity of Kimchi perceived as less salty was 2.46${\pm}$0.24% by housewives and 2.57${\pm}$0.23% by their family members, salinity of summer Kimchi perceived as appropriate was 2.61${\pm}$0.51% and 2.65${\pm}$0.57%, salinity of Kimchi perceived as salty was 2.77${\pm}$0.61% and 2.62${\pm}$0.47%, respectively. These data shows that salinity and subjective perception of salinity of summer Kimchi are correlated. The salinity of winter Kimchi perceived as less salty was 2.98${\pm}$0.83% by housewives and 3.02${\pm}$0.71% by this family members. The salinity of winter Kimchi perceived as appropriate was 2.82${\pm}$0.49% and 2.94${\pm}$0.53%, and the salinity of winter Kimchi perceived as salty was 3.20${\pm}$0.61% and 3.25${\pm}$0.49%, respectively. These data does not show salinity and subjective perception of salinity of winter Kimchi is correlated. Average salinity of summer Kimchi was 2.55${\pm}$0.44%, while average salinity of winter Kimchis was 2.97${\pm}$0.54%, which is for preservation during winter time. Furthermore, most of subjects believed that salinity of Kimchi may affects the occurrence of diseases, such as hypefention. 80% of subjects answered that it is better to eat Kimchi perceived as less salty. Therefore it is recommended to make Kimchi with below 3%o of salinity in factory scale in the near future.

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Research on Proper Dietary Recommendations for Korea (한국인(韓國人)의 균형식(均衡食) 권장에 관한 연구(硏究) -한국인(韓國人)의 식생활(食生活)의 추이(해방후 30년간)-)

  • Lee, Ki-Yull;Leekim, Yang-Cha
    • Journal of Nutrition and Health
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    • v.10 no.2
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    • pp.59-70
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    • 1977
  • This study was designed to evaluate the changes and improvements in the Korean diet during the last thirty years (since independence in 1945), and to make recommendations for the improvement of their nutritional status, consequently contributing to the physical and mental welfare of the Korean people. The results and recommendations are as follows: 1. The total calorie and carbohydrate intake decreased by $8{\sim}12%$ in the 1970's, as compared with the 1940's and the 1950's. 2. The intake of calcium and vitamiu A increased $30{\sim}50%$ and $20{\sim}60%$ respectively in the 1960's and 1970's as compared with the 1940,s. But this intake level is still lower than the RDA values. 3. The vitamin C intake was somewhat higher in the mountainous and farming areas than in urban areas. 4. In the 1970's, the decrease of untriend intake due to seasonal variation was marked especially for protein, niacin, vitamin $B_1$, and vitamin C. 5. The consumption of protein foods (meats and legumes) increased in an amount of $15{\sim}36g$ per day. There was a marked increase in the intake of meat in farming areas and of milk in urban areas in the 1970's. This increased intake of animal proteins is a very desirable dietary change. 6. The cereal consumption was lowest in urban areal, but there was a general decrease in the intake of the cereal group in the entire area in the 1970's. For the farmers, the intake of cereal food decreased most, from 750 g to 576 g, but cereals still composed a high proportion of the entire diet. 7. Fruits and vegetables showed the highest intake for the urban people, as expected. For the whole area, this food group showed an increase of 8.7% in the 1970's, as compared with the 1960's. 8. The gradual ihcrease in the intake of the fats and oil group was a desirable dietary change. but the absolute amount was too low. 9. A 7% increase in height and a 9% incrrase in weight for growing children and adolescents was observed in the 1970's as compared with the 1940's, but several kinds of deficiency diseases, such as nutritional anemia and dental caries were still apparent in many areas. 10. To improve cur food life and to cope with food shortages faced in Korea, an efficient and nationwide nutrition education program should be implemented. This would maximize efficiency of intake from the limited food sources for a balanced diet. 11. As it is of utmost importance to provide growing children with a desirable physical, sccial mental, and especially nutritional environment, a well-planned and organized school feeding program should be practiced widely and efficiently. 12. Young mothers and pregnant women should be educated on the importance of their children's nutrition, especially for the critical fetal and infancy periods. 13. More thorough and continuous nutritional survey studies on the changes in dietary patterns for the entire nation should be pursued, evaluated and documented. This would Provide a good information guide for future nutritional study programs. 14. It is the nutritionistist's strong desire that national leaders, especially decision makers recognize the fact that improvement of the nutritional status of the People is one of the most economic and preventative ways of improving their physical and mental health. This is closely related to the economic development and strength of the nation.

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The development direction of vocational education teachers' fostering of china based on vocational teachers specialization and vocational disciplines (직업교사 전문화 및 직업과학 학과발전에 기반한 중국 직업교육 교사양성 전망 -UNESCO '국제 직업교사 석사 교육과정 구성표준'을 중심으로-)

  • Yin, Zi-Long;Zhao, Zhi-Qun;Nam, Seung-Kwon;Choi, Won-Sik
    • 대한공업교육학회지
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    • v.35 no.2
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    • pp.70-81
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    • 2010
  • The purpose of this study is to introduce formation 'International framework curriculum for a Master Degree for TVET teachers and lectures' to present implications about fostering Chinese vocational teachers and to analyze the contents related to it. In 2004, UNFSCO composed formation International framework curriculum for a Master Degree for TVET teachers and lectures ("framework curriculum") to improve the ability of professionals in the vocational education and training fields including teachers and training leaders as well as to promote international academic exchange. Universities which introduce the framework curriculum should form specialized committee and carry out education considering the specific situation including other universities' situation, students' ability, educational certification system, etc. The framework curriculum should include the latest trends of the development of international vocational education science and carry out united educational learning between several internal or external high schools. UNFSCO tries to promote the development of educational learning and study of basic departments of vocational education such as vocational educational learning theory, vocational science, etc through the framework curriculum and to improve knowledge of vocational educational teachers and realize specialization of them. The number of universities that established the master's degree of vocational education in China is approx. 20 and the number of students that they collect every year. As for the plans of the master's degree of vocational teachers in each university, the courses about the practical problems like educational courses and educational learning are insufficient. But the framework curriculum thinks that educational learning of application theory is more important and emphasizes practice about the specific area and educational learning much more. Utilization of preceding experiences of advanced countries has the important meaning in search of models that foster Chinese vocational teachers and departmental system. The framework curriculum implies several useful points in installment of majors and educational process of the process that fosters Chinese vocational teachers.

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Florida, USA Food-Related Lifestyle Segments of Older Consumers in Seoul and Its Characteristics (서울지역 고령소비자의 식생활 라이프스타일에 근거한 시장세분화 및 특성 규명)

  • Jang, Yoon-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.1
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    • pp.146-153
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    • 2010
  • The objectives of this study were to explore food-related lifestyle segments of the older consumers, to identify its socio-demographic characteristics, and to investigate the differences in variables regarding health beliefs. A survey was conducted of adults 55 years of age and older living in Seoul, South Korea from March 28 to April 10, 2007. Out of the 500 distributed questionnaires, 361 were retained for final analysis: a response rate of 72.2%. As a result of cluster analysis, five consumer segments were identified; health-managing group, diet-unconcerned group, convenience-oriented group, taste-oriented group, unpracticed group. Significant differences were found among the five segments in terms of socio-demographic characteristics and variables regarding health beliefs (i.e., perceived self-efficacy, perceived barriers, perceived benefits). In the health-managing group and taste-oriented group, mean scores of perceived self-efficacy (p<0.001) and perceived benefits (p<0.001) were significantly higher than other groups. However, in the diet-unconcerned group and convenience-oriented group, the mean scores of perceived barriers (p<0.01) were significantly high. This study shows that foodservice operators targeting the older consumers should consider characteristics of each segment to develop a customized program.

Nutritional Effects of Paper Board Sludge on the Garlic(Allium sativum L.) (마늘에 대(對)한 판지(板紙) SLUDGE의 영양학적(營養學的) 연구(硏究))

  • Kim, Moon-Kyu;Chang, Ki-Woon;Choi, Woo-Young;Lee, Chang-Jun
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.3
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    • pp.208-213
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    • 1990
  • The parer board sludge (PBS) itself and compost sludge manure (CSM) made of sawdust, fowl droppings, and urea to the PBS were treated to garlic to determine the effects of growing characters, yield components, and nutritional compounds in the plants. 1. The differences of the rate of missing plants were not found between control and treatments. 2. Plant height of the garlic increased in the treatments of PBS and CSM rather than control. Numbers of leaf, and width of the largest leaf and stem did not have any distinct tendency to change. 3. Total weight and weight of the above ground part were highest in the treatment of 1,600kg/10a PBS, and CS-2 treatment mixed with high portion of additives. The weight of underground part and yield increased with the increase of paper board sludge, and highest in CS-2 treatment. 4. The contents of nitrogen in the plant were higher in the treatments of PBS and CSM than control. And CSM treatments had generally higher concentrations than PBS treatments. 5. In conclusion, PBS itself had nutritional effects. The PBS could be used to some crops after fermentation with adequate additives.

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Comparative Evaluation of Real-Time PCR and Conventional Culture Method Using Two Selective Agars for the Detection of Cronobacter spp. in Powdered Infant Formula and Dried Pumpkin (조제분유와 건조호박에서 Cronobacter spp. 검출을 위한 두 가지 선택배지와 Real-time PCR의 비교검증)

  • Kim, Hong-Seok;Shin, Minjung;Chon, Jung-Whan;Lim, Jong-Soo;Kim, Young-Ji;Kim, Dong-Hyeon;Chang, Ho-Seok;Kim, Hyunsook;Om, Ae-Son;Oh, Deog-Hwan;Song, Kwang-Young;Seo, Kun-Ho
    • Journal of Food Hygiene and Safety
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    • v.31 no.6
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    • pp.439-444
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    • 2016
  • In the present study, the performance of culture methods using two selective agars and real-time PCR were compared for selective isolation of Cronobacter in powdered infant formula and dried pumpkin. Two food samples were spiked with the pathogen and then preenriched in distilled water. A small portion of preenrichment (10 mL) was incubated in Enterobacteriaceae enrichment both, followed by inoculation onto Druggan-Forsythe-Iversen agar (DFI agar) and Cronobacter sakazakii chromogenic plating agar (R&F agar). The preenrichment and enrichment (1 mL each) was used in real-time PCR assay. In powdered infant formula (PIF), no statistical difference was observed between both culture methods and real-time PCR with preenrichemt (p > 0.05). However, the number of positives obtained by R&F agar and real-time PCR was much higher than that of culture method using DFI agar in dried pumpkin (p < 0.05). In particular, R&F agar yielded a significantly greater selectivity than DFI agar in dried pumpkin (p < 0.05). Real-time PCR and R&F agar, which are currently recommended by US FDA, could be used as an alternative detection tools for the isolation of Cronobacter in PIF and ingredient of child foods such as dried pumpkin that has high number of competing natural microflora.