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Studies on Changes of the Blood Pictures and Serum Components according to the Gestation Period in Rabbits (가토(家兎)의 임신기간(妊娠期間)에 따른 혈액상(血液像)과 혈청성분(血淸成分)의 변화(變化)에 관(關)한 연구(硏究))

  • Lee, Kyu Seung;Suh, Gil Woong
    • Korean Journal of Agricultural Science
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    • v.6 no.2
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    • pp.148-157
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    • 1979
  • The followings are the results obtained from the investigation of blood pictures and serum components of rabbits according to gestation period. 1. The erythrocytes count and hematocrit values were insignificantly decreased with the progress of gestation period. The hemoglobin content was significantly decreased at 3- and 4-week after gestation. 2. The total leukocytes count was continuousely increased during the gestation period. This tendency was significantly recognized at 3- and 4- week after gestation, but the normal situation was restored after parturition. While the percentage of neutrophils was significantly increased, that of lymphocytes was decreased from 3 weeks after gestation. 3. The contents of total protein and non-protein nitrogen were continuously decreased with the process of gestation period, but the significant differences were recognized from 3 weeks. 4. The total lipids were not markedly changed until 3 weeks, but significantly increased at 4-week after gestation and on 5-day after parturition. 5. The serum cholesterol tended to be decreased until 3 weeks, but significantly increased at 4-week after gestation and on 5-day after parturition. 6. The serum calcium was continuousely decreased during the gestation period, but the significant differences were recognized at 3- and 4-week. The serum phosphorus was also significantly decreased at 4-week after gestation.

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Studies on the Antibacterial Activity of Enzymatic Hydrolyzates of Lactoferrin Derived from Bovine Colostrum (유우 Lactoferrin 효소가수분해물 항균 활성에 관한 연구)

  • Han, Su Yeon;Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.25 no.1
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    • pp.52-67
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    • 1998
  • The investigative research on the mammalian milk purely consisted of the physiological quality of lactoferrin was conducted to reveal the antimicrobial ativity of specifically functional foods with antibiotic characteristics as a basic data in food manufacturing. Bovine lactoferrin were isolated from raw milk samples, and was digested with pepsin, trypsin and chymotrypsin. It was necessary then to separate and purify lactoferrin from bovine raw milk, and in order to analyze the antimicrobial activity of the enzyme-treated bovine lactoferrin in their required quantitative fraction. Afterwards Escherichia coli and Staphylococcus aureus was incubated in it. It was that investigated to enzyme-treated fractions molecular weight and the peptide fragment with antimicrobial effect. 1. The purity of enzyme-treated bovine lactoferrin(BLF) was tested by SDS-PAGE. As a results of 12% SDS-PAGE assay, pepsin-treated LF did not exhibited band until if reaches 14 KDa, while trypsin and chymotrypsin treated LF, known to contain the non-digestive lactoferrin exhibited band at a molecular weight of 33 KDa. 2. Bovine lactoferrin was sucessfully purified through the use of Sephadex G-50 Column. In order to assay LF through the Sephadex G-50 column chromatography, the digestive bovine lactoferrin (BLFs) was eluted with a linear gradient of 0.05% Tris-HCl. When the gel-filtration analysis, pepsin, trypsin and chymotrypsin treatments of BLF fragments was showed 2, 3, and 2 peak, respectively. The results of the HPLC analysis confirmed that had a non-digestive lactoferrin receptor, and trypsin and chymotrypsin treated BLFs has an antimicrobial effect. 3. To measure the strength of the antimicrobial effect of enzyme treated lactoferrin it was compared to the antimicrobial activity taking place at the incubated Escherichia coli and Staphylococcus aureus. This might explain the resistance of the microorganisms for peptide fragment. The pepsin-treated of bovine lactoferrin was markedly reduced by incubation of the cells. Trypsin-treated of BLF was similar to chymotrypsin-treated of BLF. However, trypsin and chymotrypsin treatments of BLFs were showed the antimicrobial effect until eight hours incubation for native bovine lactoferrin. Therefore the enzyme-treated lactoferrin have an antimicrobial effect even non-digestive lactoferrin. 4. The digestive bovine lactoferrin fragments assay was carried out by the use of Sephadex G-50 column chromatography and SDS-PAGE. The pepsin and chymotrypsin-treated fragments has a low molecular weight and trypsin-treated lactoferrin was only showed a band. It was described that characteristics of digestive protein. It appeared that there may be a relation between virulence and resistance to enzyme-treated BLF.

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Effects of Pre-heat Treatments on Milk Protein and Microorganism Aspects in Raw Milk (예열처리(豫熱處理)가 원유(原乳)의 단백질(蛋白質) 및 미생물(微生物)에 미치는 영향(影響))

  • Kim, Seung Sub;Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.20 no.2
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    • pp.153-166
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    • 1993
  • The experiments were conducted to improve raw milk quality during storage, the chemical composition and microbiological aspect of raw milk, milk thermized at $65^{\circ}C$ for 30 second, and $75^{\circ}C$ for 2 second stored at $5^{\circ}C$ for 4 days were investigated. The result obtained were summarized as follows : 1. During storage of raw and thermized milk, in the composition of milk fat, milk protein, lactose and total solid did not change significantly. 2. The range of pH and acidity for the raw milk were 6.73~5.94 and 0.16~0.27% respectively and those of the thermized milk were 6.79~6.62 and 0.16~0.17% respectively. The phosphatase test were negative in heated milk. 3. The composition of total nitrogen, NCN and whey protein were decreased, wherease those of NPN and casein were increased in heat treated milk. 4. The compositions of total nitrogen and casein were decreased as the storage period advanced, wherese those of NCN and NPN were increased. However, the composition of whey protein did not significantly change. 5. The number of coliform bacteria was not found in thermized milk. but were gradually increased in raw milk during storage period. 6. Raw milk had total bacteria count as $5.6{\times}10^6/ml$, psychrotrophic bacteria $1.8{\times}10^6/ml$ and thermoduric bacteria $1.6{\times}10^5/ml$, as the heat treatment increased microorganism counts decreased to milk thermized at $75^{\circ}C$, for 2 sec. $3.0{\times}10^3/ml$, $1.5{\times}10/ml$ and $2.7{\times}10^3/ml$ respectively. 7. The count of thermoduric bacteria, psychrotrophic bacteria and total bacteria were increased during storage period, and more rapidly increased in raw milk than in heat treated milk.

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Effect of storage condition on sensory properties and fatty acid composition of pre-packed Hanwoo loin (저장조건이 포장 한우 등심의 관능적 특성 및 지방산 조성에 미치는 영향)

  • Seol, Kuk-Hwan;Kim, Ki Hyun;Kim, Young Hwa;Youm, Kyung Eun;Lee, Mooha
    • Korean Journal of Agricultural Science
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    • v.42 no.1
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    • pp.37-46
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    • 2015
  • This study was carried out to determine the effect of storage condition, such as temperature and relative humidity (RH), on the change of sensory properties and free fatty acid composition of pre-packed Hanwoo loin (Musculus longissimus) until $21^{st}$ day of storage. The Hanwoo loin was sliced in $1.0{\pm}0.2cm$ thickness and each of the sliced pieces was packed in a foamed polystyrene tray with linear low-density polyethylene (LLD-PE) film to simulate the pre-packed Hanwoo loin sold in retail market, then stored in home-style refrigerator (Con; $5^{\circ}C$, 17%RH), and temperature and RH controled chambers (T1; $5^{\circ}C$, 55%RH, T2; $5^{\circ}C$, 85%RH, and T3; $-1^{\circ}C$, 99%RH), respectively. The sensory characteristics of pre-packed Hanwoo loin during storage in different conditions were measured on raw or after cooking. After 21 days of storage, the loin stored in T3 showed significantly high color and acceptability scores ($7.46{\pm}1.07$, $7.06{\pm}1.36$, respectively) on raw loin, and flavor and acceptability scores ($7.16{\pm}0.90$, $6.90{\pm}0.92$, respectively) on cooked loin than those of others (p<0.05). The concentration of 12 fatty acids of pre-packed Hanwoo loin during storage in different conditions were analysed. The concentration of oleic acid (C18:1, $39.92{\pm}3.22%$), palmitic acid (C16:0, $28.20{\pm}2.42%$), and stearic acid (C18:0, $12.59{\pm}2.79%$) was high in Hanwoo loin on $1^{st}$ day of storage. The concentration of palmitic acid of Hanwoo loin stored in T2 was increased significantly after $4^{th}$ day of storage (p<0.05), and the concentration of myristic acid(C14:0) of Hanwoo loin stored in treated chamber (T1 to T3) was significantly higher than Con after $14^{th}$ day of storage (p<0.05). The concentration of linolenic acid (C18:3) of Hanwoo loin was decreased significantly after $4^{th}$ day of storage in all tested storage conditions (p<0.05). However, there was no significant or consistent change in the concentration of oleic acid (C18:1) during storage period in every Hanwoo loin stored in tested conditions. Although, the concentration of total monounsaturated fatty acids (MUFA) was decreased in Con and T2 (p<0.05), there was no consistent change in the monounsaturated fatty acids to saturated fatty acids ratio (MUFA/SFA) during storage period (p>0.05). From those results, it was considered that storage condition affects on the sensory acceptability and fatty acid composition of pre-packed Hanwoo loin as storage period was increased.

Searching for the SCM Improvement Directions through the Power Attribute and Partnership (파워 유형과 파트너십 연계를 통한 공급사슬관리 개선방안 모색)

  • Jung, Dae-Hyun;Park, Kwang-O
    • Management & Information Systems Review
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    • v.35 no.3
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    • pp.57-79
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    • 2016
  • It is required to derive various conclusions by identifying the type of power and the relationship between SCMs and presenting practical implications. Thus, we can identify the differential effects of each type of power on SCM performance. We can contribute to develop the practical implications at more sophisticated multi-dimension by comparing results of this study with various SCM theories. Through previous studies, the source of power is largely divided into binding power and non-binding power. Binding power is classified into behavior coercion, binding reward and relationship legitimacy. Non-binding power is classified into work expertise, information superiority and value compliance. Enterprises should fully understand and recognize partners within supply chains including understanding of the source of power, imbalance and results. Thus, we look into types of power and effects on trust and commitment, and identify a causal relationship leading to collaboration and SCM performance. Specific research results are as follows. First, the binding power did not give a significant effect to the trust. However, the binding power gave a positively(+) significant effect to the commitment. Second, non-binding power showed a significant effect on both trust and commitment. As a result of analysis on total effects, it was shown that non-binding power gave indirect effects to collaboration and SCM performance. Third, it was shown that both trust and commitment significantly affected collaboration. From the perspectives of social exchange theory and trading cost theory among inter-organizational relationship theory, it may lead to SCM performance of trust, commitment and collaboration. Moreover, it was found that association of each attribute of power led to the significant result. Fourth, it was shown that trust and collaboration significantly affected SCM performance. However, commitment did not directly affect SCM performance, but it indirectly significantly affected SCM performance through collaboration. Proper use of this power can firmly build partnerships between members of the supply chain and induce the improvement on supply chain performance and satisfaction of members.

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Effects of CSV Activities on Purchasing Intention : on the Perspectives of Value Chain (공유가치창출(CSV)활동이 구매의도에 미치는 영향 : 가치사슬 관점)

  • Weon, Jong-Ha;Jung, Dae-Hyu
    • Management & Information Systems Review
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    • v.36 no.4
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    • pp.1-19
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    • 2017
  • These days, the concept of creating shared value is drawn keen attentions to. This interest comes out of the expectation that Creating Shared Value(CSV) can offer an answer to some social issues by creating societal and economic values on the top of the achievements that existing Corporate Social Responsibility(CSR) has made. However, it is difficult to make a clear distinction between the achievements that the activities of CSR and CSV have made. In this regard, developing a methodology to make an actual proof analysis on the accomplishments of CSV and to verify customer's awareness of and attitude towards the CSV is necessarily required. A company needs to gain a competitive advantage in the marketplace as well as resolve a social issue by innovating value chain. The research has verified the cause and effect relationship between the CSV from the point of view of value chain and the purchase intention aroused by its economic, societal and cultural values through the company image and credibility with actual proof analysis and come up with following results. First, a societal and cultural value resulted in giving positive impact on a company's image, which implies that CSV activities can be the thin end of the wedge through which customers have a good image of the company involved in CSV. Second, a societal value makes a positive influence on the credibility of a company. In this regard, CSV should be recognized not just as a thing that generates a cost, but a way to win-win as well as future development. Third and last, the research results show that both company image and credibility influence on purchase intention. Considering that CSV generates a positive evaluation on a company that will ultimately cause continuous profit-making, the company's ultimate goal of activities, it should be approached from the perspective of making a mid-and-long term strategy.

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Relation Between Oral Health Knowledge and Behavior of Mother and a Oral Health Condition of Children (일부 어머니의 구강건강 지식 및 행동과 초등학교학생들의 구강건강상태와의 관련성)

  • Choi, Sung-Suk
    • Journal of dental hygiene science
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    • v.5 no.4
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    • pp.245-250
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    • 2005
  • This study was executed from June first to 30th. 2005. The target was first and second graders of Bok-Hyun elementary school and their mothers. The total of participants was 275 persons and the component ratio was 135 first graders and 140 second graders, also 82 boys and 193 girls. They answered to the question sheet by writing out with their own hands. Among the 300 question sheets that were answered, 275 sheets were analyzed, excepting 25 sheets that got poor answer. The results were as follows; (1) About the behavior of mothers and their children for oral health, In mothers' case, 68.8% of them answered that they brush teeth more than 3 times a day and 31.3% answered that they brush teeth less than 2 times a day, so those who brush teeth more than 3 times were more than those who brush teeth less than 2 times In children's case, on the contrary, 14.2% of them answered that they brush teeth more than 3 times a day and 85.8% answered that they brush teeth less than 2 times a day, so those who brush teeth less than 2 times were more. (2) In 7 questions for researching mothers' knowledge level about fluorine, 55.3% of them answer 3~4 questions correctly. In 7 questions for researching mothers' knowledge level about Periodontal disease, 47.3% of them answer 3~4 questions correctly and it was highest. (3) In a case of the relation between oral health knowledge and behavior of mother and frequency of brushing teeth of children, statistical similarity on frequency of brushing teeth was shown(p < 0.05). (4) In a case of the relation between social-anthropological features of mother and frequency of brushing teeth of children, there was no statistical similarity on age, mothers' educational level, monthly income. However, statistical similarity was shown about existence of mothers' occupation(p < 0.05).

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Usefulness of Dental Hygiene Education Curriculum in the Public Oral Health Service Performance in Korea (한국의 보건소 근무 치과위생사의 공중구강보건사업 수행업무실태와 대학교육간의 연관성)

  • Yoo, Ja-Hea;Cho, Young-Sik;Chung, Won-Gyun;Kwon, Ho-Keun
    • Journal of dental hygiene science
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    • v.5 no.4
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    • pp.165-170
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    • 2005
  • The purpose of this study was to evaluate the usefulness of dental hygiene education curriculum such as oral prophylaxis, preventive dentistry, public oral health, oral health statistics, oral health education in the public oral health service performance. Questionnaire survey was posed to 96 dental hygienists working in health centers and health sub-centers. The relativity among frequency of task, usefulness, importance, and reinforcement of the education curriculum in the health centers, and the effect of current dental hygiene education curriculum on public oral health service were studied. The results were follows; 1. Sealant was performed the most frequently in the health centers and health sub-centers, and it was also the most useful content in the education curriculum. And the tooth brushing method was considered the most important in the curriculum. Therefore, oral health education was considered as factor which required the most reinforcement. However, the issues of school water fluoridation and water fluoridation in the public oral health were barely brought up as a matter of subject. 2. In the relationship between the frequency of task and the usefulness of curriculum, it showed that the more frequency of task was more useful. In the relationship between importance and reinforcements of curriculum, it showed that the more importance of the education curriculum requires more reinforcement. And more frequently performed task should be more strengthened. 3. According to the education course hours, current education curriculum course hours were 532.4 hours, which is 42.9 hours more spent than the original curriculum guideline. Lecture hours were 205.4 hours, which is 50.6 hours less, and the practice hours were 327 hours, which is 93.5 hours more than the original curriculum guideline. 4. Because of the insufficient course hours of curriculum compared to the frequency of the task, the oral health education, oral prophylaxis, and preventive dentistry should be reinforced more than now. But the oral health statistics and public oral health curriculum were not only emphasized, but also any reinforced compared to other tasks.

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Occluding Effect of the Application of Fluoride Compounds and Desensitizers on Dentinal Tubules (불소도포 및 지각과민처치제 적용 후 상아세관 폐쇄효과와 지속력 평가)

  • Lee, Ye-Jin;Jeong, Moon-Jin;Ahn, Yong-Soon;Cheun, Su-Kyung;Jeong, Soon-Jeong;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.16 no.4
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    • pp.272-283
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    • 2016
  • This study compared and analyzed the occluding effects of fluoride compounds and desensitizers, which are commonly used in dental clinics, on dentinal tubules. This study also evaluated the persistence of the active ingredients over time by performing toothbrushing with an electric toothbrush. Thirty-five molar teeth, which had been extracted within the past 3 months from healthy people without tooth decays, amalgam fillings, or dental crowns, were divided into 4 pieces each. Of these, 135 teeth pieces were used as study specimens. These specimens were divided into a control group, an untreated group, and 5 experimental groups (acidulated fluoride gel, fluoride varnish, Gluma, Super Seal, and SE-Bond). The specimens were then subjected to toothbrushing equivalent to 1 week (140 times), 2 weeks (280 times), and 4 weeks (560 times), and the occluding effects on dentinal tubules in 3 regions of each specimen were examined under a scanning electron microscope. The fluoride varnish treated group showed the highest degree of dentinal tubule occlusion effects during the first, second, and fourth weeks of toothbrushing, with the SE-Bond treated group showing the second highest degree and the Gluma treated group showing the lowest degree. After 4 weeks of toothbrushing, the Gluma treated group and the Super Seal treated group showed the lowest degrees of dentinal tubule occlusion effects. In summary, the fluoride varnish treated group and the SE-Bond treated group displayed higher occlusion effects even after 4 weeks of treatment than did the other experimental groups. Therefore, it is the authors' belief that fluoride varnish and SE-Bond are effective for treating dentinal hyperesthesia.

Effects of the Enamel Erosion Caused by Certain Antipyretic and Analgesic Medicines for Children (일부 어린이 해열·진통제의 유치 법랑질 부식효과)

  • Cheun, Su-Kyung;Jeong, Moon-Jin;Ahn, Yong-Soon;Lee, Ye-Jin;Ko, Mi-Kyung;Jeong, Soon-Jeong;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.16 no.3
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    • pp.235-241
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    • 2016
  • This study was conducted to provide basic understanding regarding possible enamel erosion by three kinds of fist-aid antipyretic and analgesic medicines over a period of time, with comparison and analysis of the resulting deciduous teeth surface and microhardness changes. The analysis was performed using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscope (SEM) to examine the surface erosion and changes. The Kruskal-Wallis test show differences in surface erosion and changes after 3, 5 and 8 days of treatment as well as before and after the treatment in each group. According to the results, there was no significant difference in the early deciduous teeth enamel surface microhardness (p>0.01). However there were signigicant changes after 3, 5, and 8 days (p<0.01). Calcim (Ca) and phosphorous (P) analysis using EDX showed significant differences in the enamel characteristics according to each tissue area after 8 days (p<0.05), but there was no significant difference in any of the areas for P content (p>0.05). In the surface observation with the SEM treatment with Children's Tylenol$^{(R)}$ tablet, which has the lowest pH, looked the roughest, followed by Brufen syrup for children and Children's Tylenol$^{(R)}$ suspension. Based on these results, it should be considered that antipyretic and analgesic medicines for children, which have lower pH values, may cause tooth erosion. Hence, it is necessary to give special attention to oral hygiene in young children or infants by brushing their teeth after such drugs are administered.