• Title/Summary/Keyword: scanning microscopy

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Light and Scanning Electron Microscopic Study on the Pelvic Epithelium of the Sheep Kidney (양(羊)의 신우상피에 대한 광학 및 주사전자현미경적 연구)

  • Kim, J.;Oh, S.J.;Chung, J.W.
    • Applied Microscopy
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    • v.15 no.2
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    • pp.98-110
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    • 1985
  • This study was performed to clarify the morphological structures of the epithelia of the renal papilla, renal pelvis and ureter of the sheep (Ovis aries L.) through the light and scanning electron microscopes, Tissue specimens were taken from the renal papilla (common renal papilla and peripelvic column) and the renal pelvis (pelvis proper and pelvic pouch) of the kidney and the ureter. For the light microscopy, tissue blocks were fixed in 10 % neutral buffered formalin and embedded in paraffin wax, serially sectioned at a thickness of $6{\mu}m$. These sections were stained with hematoxylin-eosin and periodic acid-Schiff reaction. For the scanning electron microscopy, tissue blocks were prefixed in 1% glutaral-dehyde-1.5% paraformaldehyde solution and postfixed in 1% osmium tetroxide solution, dehydrated in graded alcohol, transferred to isoamyl acetate, and then dried by the critical point dryer (Polaron E 3000). These dried tissues were coated with gold and observed with a scanning electron microscope (JSM-35C), The results were as follows: The apex of the common renal papilla was lined with simple columnar epithelium having many microvilli on its luminal surface. Lateral portion of the papilla was lined with stratified epithelium $2{\sim}3$ layers thick, and its superficial cells were microvillar cells having many microvilli. The epithelium lining the peripelvic column was $1{\sim}2$ layers thick. The superficial layer was made of the microvillar cells, but a few microplica cells were appeared in the region near the pelvic pouch. The epithelium of the pelvic pouch was $1{\sim}2$ layered transitional type, and its superficial cells were microplica cells. The epithelia of the pelvis proper and ureter were $4{\sim}6$ layered transitional type, and their superficial cells were typical facet cells existing many round depressions and ridges of cell membranes of the luminal side.

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Quantitative Method to Measure Thermal Conductivity of One-Dimensional Nanostructures Based on Scanning Thermal Wave Microscopy (주사탐침열파현미경을 이용한 1 차원 나노구조체의 정량적 열전도도 계측기법)

  • Park, Kyung Bae;Chung, Jae Hun;Hwang, Gwang Seok;Jung, Eui Han;Kwon, Oh Myoung
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.38 no.12
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    • pp.957-962
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    • 2014
  • We present a method to quantitatively measure the thermal conductivity of one-dimensional nanostructures by utilizing scanning thermal wave microscopy (STWM) at a nanoscale spatial resolution. In this paper, we explain the principle for measuring the thermal diffusivity of one-dimensional nanostructures using STWM and the theoretical analysis procedure for quantifying the thermal diffusivity. The SWTM measurement method obtains the thermal conductivity by measuring the thermal diffusivity, which has only a phase lag relative to the distance corresponding to the transferred thermal wave. It is not affected by the thermal contact resistances between the heat source and nanostructure and between the nanostructure and probe. Thus, the heat flux applied to the nanostructure is accurately obtained. The proposed method provides a very simple and quantitative measurement relative to conventional measurement techniques.

Analysis on the Preservation of Scalp Collected from Full-Term Baby Mummy of Medieval Joseon Dynasty (조선시대 태아 미라 두피조직의 보존상태 분석)

  • Chang, Byung-Soo
    • Applied Microscopy
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    • v.38 no.2
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    • pp.135-140
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    • 2008
  • In this study, I investigated on the ultrastructure of scalp skin from full term baby mummy by using transmission and scanning electron microscopy. The baby mummy was found within the uterus of a 16th century (Joseon Dynasty) mummified woman aged 20${\sim}$30 years old. In scanning electron microscopic study, I found that the outer surface of scalp skin containing of sweat gland and stratum corneum are well preserved. The skin of the scalp measured about 1 mm in thickness and the epidermis was well distinguished from the dermis. In transmission electron microscopy, the shape and structure of the epithelial cells were not confirmed. I also observed well preserved collagen fibers composed of collagen fibrils with cross banding pattern ultrastructurally. But, the other connective cells were not observed due to decomposition of the dermis.

Study on the Tensile Strength of Bleached Hair (탈색모발의 인장강도에 관한 연구)

  • Lee, Gui-Young;Chang, Byung-Soo
    • Applied Microscopy
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    • v.38 no.3
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    • pp.251-257
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    • 2008
  • Human hair experience tensile forces during combing, bleaching and permanent dyeing processes. We have used rheometer to characterize bleached hair tensile response. Also, we investigated to morphological changes after tensile test of virgin hair and bleached hair using scanning electron microscopy. In tensile test, the tensile strength of virgin hair was $14.66g/cm^2$. They were measured as $12.95g/cm^2$, $12.61g/cm^2$ and $11.43g/cm^2$ for after the first, the second and the third trials of bleaching were done, respectively. Virgin hair had a higher tensile force than bleached hair. Tensile strength got lowered as more bleaching trials were done. In the observation of scanning electron microscopy, the fracture and lift-off of the cuticle outer layer were shown in virgin hair and bleached hair. The bleached hair were more lifted off at the outer cuticle layer than virgin hair. Consequently, chemical damage of bleaching agent caused weakening of the outer cuticle in the hair.

New Measurement of Whitening Effects by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 신규 미백 효과 측정 연구)

  • Kim, Myong Ki;Cho, Seok-Cheol;Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.279-285
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    • 2015
  • Hyperpigmentation on face is a highly anxiety-producing symptom, especially for women from the aspect of beauty. Pigmentation of the skin is related to the amount of melanin that provides protection against UV radiation. In vivo reflectance confocal microscopy is a non-invasive imaging tool allowing visualization of the skin without tissue alteration, by placing a microscopy directly on the living skin. The aim of this study was to develop the new evaluation method of whitening effects using in vivo reflectance confocal microscopy and to validate other instruments for measuring skin colors, and UV-induced hyperpigmentation was elicited on the inside skin of the forearm. It suggested that the new method for whitening effects using the confocal microscopy was useful to evaluate the de-pigmentation products and to easy for understanding to customers.

Adsorption of H Atoms on the Si(111)$4{\times}1$-In Surface (Si(111)$4{\times}1$-In 표면에의 수소원자 흡착 연구)

  • Yu Sang-Yong;Lee Geun-Seop
    • Journal of the Korean Vacuum Society
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    • v.15 no.2
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    • pp.139-144
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    • 2006
  • Using scanning tunneling microscopy (STM) measurements, we studied the adsorption of hydrogen on the Si(111)$4{\times}1$-In surface at room temperature. The H atom features are found to be located between the two protrusions in one side of the $4{\times}1$ chain. The adsorbed H preferentially occupies one of the two zigzag In subchains, suggesting that the adsorption of H is influenced by the subsurface structure. The adsorbed H atom induces not only a localized distortion but also perturbs the distant region and results in a period-doubling modulations in the STM images. This H-induced perturbation differs from the Na-Induced perturbation on the same surface.

Drop-weight impact damage evaluation for carbon fiber/epoxy composite laminates (탄소 섬유강화 복합재료의 중력 낙하 충격으로 인한 손상 평가)

  • Sohn, Min-Seok;Hu, Xiao-Xhi;Ki, Jang-Kyo;Hong, Soon-Hyung
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2001.05a
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    • pp.89-92
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    • 2001
  • Drop weight impact tests were performed to investigate the impact behavior of carbon fiber/epoxy composite laminates reinforced by short fibers and other interleaving materials. Characterization techniques, such as cross-sectional fractography and scanning acoustic microscopy, were employed quantitatively to assess the internal damage of some composite laminates. Scanning electron microscopy was used to observe impact damage and fracture modes on specimen fracture surfaces. The results show that composite laminates experience various types of fracture; delamination, intra-ply cracking, matrix cracking and fiber breakage depending on the interlayer materials. Among the composite laminates tested in this study, the composites reinforced by Zylon fibers showed very good impact damage resistance with medium level of damage, while the composites interleaved by poly(ethylene-co-acrylic acid) (PEEA) film is expected to deteriorate the bulk strength due to the reduction of fiber volume fraction, even though the damaged area is significantly reduced.

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Scanning Electron Microscopic Study on the Head Lice Eggs Detected in Korean Children

  • Park, Mi Soon;Chang, Byung Soo
    • Applied Microscopy
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    • v.44 no.2
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    • pp.47-52
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    • 2014
  • Fine structural characteristics of eggs (nits) found on the scalp hairs of Korean children were observed with scanning electron microscopy. An egg is structurally composed of four parts: the cementum, nit body, operculum, and aerophyle. The total length of an egg is about 1.5 mm. The glue secreted from the female louse completely surrounds the 1 mm hair shaft and forms the cementum. The thickness of the cementum at the end of the nit body was found to be more than 5 times the thickness at the end toward the scalp. The nit body is shaped like a goblet with a very smooth surface. The operculum and aerophyles are located at the apiculus area. In the circular operculum, there are 10 aerophyles concentrated on the side of the hair shaft surface that are shaped like a dome. Three aerophyles in the center are surrounded by 7 aerophyles. Each aerophyle is dome shaped with a diameter of $65{\mu}m$ and a respiratory pathway with a $15{\sim}25{\mu}m$ opening at the center. On the cut surface where the operculum is separated as the egg hatches, long grooves about $1{\mu}m$ thick are uniformly formed transversely. These long grooves facilitate the separation of the operculum through body expansion at the time of hatching.

Phase Transition of Octaneselenolate Self-assembled Monolayers on Au(111) Studied by Scanning Tunneling Microscopy

  • Choi, Jung-Seok;Kang, Hun-Gu;Ito, Eisuke;Hara, Masahiko;Noh, Jae-Geun
    • Bulletin of the Korean Chemical Society
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    • v.32 no.8
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    • pp.2623-2627
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    • 2011
  • We investigated the surface structure and wetting behavior of octaneselenolate self-assembled monolayers (SAMs) on Au(111) formed in a 50 ${\mu}M$ ethanol solution according to immersion time, using scanning tunneling microscopy (STM) and an automatic contact angle (CA) goniometer. Closely-packed, well-ordered alkanethiol SAMs would form as the immersion time increased; unexpectedly, however, we observed the structural transition of octaneselenolate SAMs from a molecular row phase with a long-range order to a disordered phase with a high density of vacancy islands (VIs). Molecularly resolved STM imaging revealed that the missing-row ordered phase of the SAMs could be assigned as a $(6{\times}{\surd}3)R30^{\circ}$ superlattice containing three molecules in the rectangular unit cell. In addition, CA measurements showed that the structural order and defect density of VIs are closely related to the wetting behaviors of octaneselenolate SAMs on gold. In this study, we clearly demonstrate that interactions between the headgroups and gold surfaces play an important role in determining the physical properties and surface structure of SAMs.

STM Studies of Keggin-type and Wells-Dawson-type Heteropolyacid Catalysts (Keggin 형 및 Wells-Dawson 형 헤테로폴리산 촉매의 STM 연구)

  • Park, Gyo Ik;Barteau, Mark A.;Jung, Ji Chul;Song, In Kyu
    • Korean Chemical Engineering Research
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    • v.47 no.2
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    • pp.163-168
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    • 2009
  • Negative differential resistance(NDR) behaviors of Keggin-type and Wells-Dawson-type heteropolyacids with cation, heteroatom, and polyatom substitutions were investigated by scanning tunneling microscopy. A reliable correlation between NDR peak voltage and reduction potential of heteropolyacid catalysts was established. It was found that more reducible heteropolyacid catalyst showed NDR behavior at less negative voltage, regardless of the structural difference. Thus, NDR peak voltage of heteropolyacid catalyst could be utilized as a single correlating parameter for the reduction potential of heteropolyacid catalyst.