• 제목/요약/키워드: scanning electron microscope

검색결과 3,241건 처리시간 0.033초

수치해석을 통한 SEM 챔버내의 이차전자 거동해석 및 이차전자 검출기의 최적 장착 위치 선정 (The Behavior of Secondary Electrons and Optimal Mounting Position of a Secondary Electron Detector in SEM with a Numerical Analysis)

  • 부경석;전종업
    • 한국공작기계학회논문집
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    • 제17권4호
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    • pp.15-21
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    • 2008
  • Secondary electron detectors used in scanning electron microscope accept secondary electrons emitted from the specimen and convert them to an electrical signal that, after amplification, is used to modulate the gray-level intensities on a cathode ray tube, producing an image of the specimen. In order to acquire images with good qualities, as many secondary electrons as possible should be reached to the detector. To realize this it is very important to select an appropriate mounting position and angle of the detector inside the chamber of scanning electron microscope. In this paper, a number of numerical simulations are performed to explore the relationships between detection rates of secondary electrons and the values of some parameters, such as distances between the detector and sample, relative mounting positions of scintillator positioned inside the detector with respect to detector cover, two types of mounting angles of the detector. The relationships between detection rates and applied voltages to corona ring and faraday cage, and energies of secondary electrons are investigated as well.

Specimen Preparation for Scanning Electron Microscope Using a Converted Sample Stage

  • Kim, Hyelan;Kim, Hyo-Sik;Yu, Seungmin;Bae, Tae-Sung
    • Applied Microscopy
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    • 제45권4호
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    • pp.214-217
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    • 2015
  • This study introduces metal coating as an effective sample preparation method to remove charge-up caused by the shadow effect during field emission scanning electron microscope (FE-SEM) analysis of dynamic structured samples. During a FE-SEM analysis, charge-up occurs when the primary electrons (input electrons) that scan the specimens are not equal to the output electrons (secondary electrons, backscattered electrons, auger electrons, etc.) generated from the specimens. To remove charge-up, a metal layer of Pt, Au or Pd is applied on the surface of the sample. However, in some cases, charge-up still occurs due to the shadow effect. This study developed a coating method that effectively removes charge-up. By creating a converted sample stage capable of simultaneous tilt and rotation, the shadow effect was successfully removed, and image data without charge-up were obtained.

Fusarium moniliforme 감염벼종자의 소독과 주사전자현미경적 조직관찰 (Effect of Seed Treatment and Observation of Seeds Infested with Fusarium moniforme by Scanning Electron Microscope)

  • 성재모;이형순;유승헌;신관철
    • 한국식물병리학회지
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    • 제1권1호
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    • pp.51-55
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    • 1985
  • Fusarium moniliforme에 감염된 벼줄기의 도관부에서도 균사가 관찰되었다. Benlate T와 Busan 30을 처리한 종자에서도 소독시간에 관계없이 F. moniliforme가 분리되지 않아 소독효과가 인정되었으나 현미에서 소독시간에 관계없이 F. moniliforme가 검출되어 방제효과가 떨어졌다.

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Bacteria를 이용한 연약한 흙의 고결화 가능성 (Possibility of cementation of soft soil using Bacteria)

  • 김대현;김호철;박경호
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2010년도 추계 학술발표회
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    • pp.379-391
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    • 2010
  • In order to understand the mechanism of cementation of soft soils treated with bacteria, three types of specimens(untreated, normal bacteria concentration treated, and high bacteria concentration treated) were made. Scanning Electron Microscope(SEM), EDX and X-ray diffraction(XRD) analyses were performed on the soft silt and loose sand specimens. Compared with the untreated specimen, a clearer cementation between particles was observed in the high bacteria concentration treated specimen. Based on the scanning electron microscope(SEM) EDX analyses, more calcium carbonate was observed in the specimen treated with high bacteria concentration than other specimens. On the basis of the preliminary results, it appears that microbial cementation can occur in the soft soil. Further study on the cementation of soils using bacteria is necessary to validate this result.

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자동차용 스틸휠 디스크부품의 성형불량 및 파손사례분석 (Analysis of Damaged Instance and Forming Fault for Disc Part in Automotive Steel Wheel)

  • 이성희;김무연;김태규;윤호영;강석우
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2006년도 춘계학술대회 논문집
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    • pp.234-238
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    • 2006
  • In this research, an analysis of damaged instance and forming fault for disc part in automotive steel wheel was performed. Rolled steel material, which had been used in the manufacturing of the damaged disc part, was prepared for tensile test, quantitative analysis of chemical component and acquirement of scanning electron microscope images. Although the results of mechanical properties and chemical component ratio for the material satisfied the suggested specification, some material inherent problem was found in the scanning electron microscope images. Finally, in an analysis of chemical component for the damaged disc part used in road condition, mismatching of chemical component ratio between the suggested specification and test result was found.

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신경망과 주사전자현미경을 이용한 플라즈마 진단 (Plasma Diagnosis by Using Scanning Electron Microscope and Neural Network)

  • 배중기;김병환
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2006년도 심포지엄 논문집 정보 및 제어부문
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    • pp.96-98
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    • 2006
  • A new ex-situ model to diagnose a plasma processing equipment was presented. The model was constructed by combining wavelet, scanning electron microscope, ex-situ measurement of etching profile, and neural network. The diagnosis technique was applied to a tungsten etching process, conducted in a $SF_6$ helicon plasma. The wavelet was used to characterize detailed variations of plasma-etched surface. The diagnosis model was constructed with the vertical wavelet component. For comparison, a conventional model was built by using the estimated profile data. Compared to the conventional model, the wavelet-based model, demonstrated a much improved diagnosis.

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의치상 레진의 중합 방법에 따른 크기의 안정성 및 표면 형태에 관한 연구 (DIMENSIONAL STABILITY AND SURFACE MORPHOLOGY OF VARIOUS DENTURE RESINS)

  • 채숙영;방몽숙
    • 대한치과보철학회지
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    • 제30권3호
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    • pp.401-410
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    • 1992
  • The purpose of this study was to investigate the dimensional changes and surface morphology of dentures processed by various polymerization conditions. The measurements were done by taking radiograph and using vernier calipers and each specimen was observed on scanning electron microscope. Results obtained were as follows. 1. The difference of dimensional stability was not recognized between various polymerization conditions(heat-cured resin, pour-type resin, microwave-cured resin, and injection molding resin). 2. There were expansion and shrinkage in the occlusal dimension, shrinkage in the frontal dimension, and expansion in the lateral dimension. 3. Scanning electron microscope pictures of heat-cured resin showed dense and regular surface morphology. 4. Microwave-cured resin surface appeared more regular and smooth than pour-type resin but less dense and more irregular than heat-cured resin. 5. Scanning electron microscope pictures of pour-type resin with the lowest dimensional change showed the most irregular surface morphology.

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Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

  • Jihun Kang;Eun-Hye Kang;Young-Shik Yun;Seungmuk Ji;In-Sik Yun;Jong-Souk Yeo
    • Applied Microscopy
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    • 제50권
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    • pp.26.1-26.3
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    • 2020
  • The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500- nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur

  • Shin, Yong Ho;Ko, Eun Ju;Kim, Su Jeong;Hyun, He Nam;Jeun, Yong Chull
    • The Plant Pathology Journal
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    • 제35권5호
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    • pp.417-424
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    • 2019
  • Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides