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Identification of Genes Encoding Heat Shock Protein 40 Family and the Functional Characterization of Two Hsp40s, MHF16 and MHF21, in Magnaporthe oryzae

  • Yi, Mi-Hwa;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.24 no.2
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    • pp.131-142
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    • 2008
  • Magnaporthe oryzae, the causal agent of the rice blast disease, poses a worldwide threat to stable rice production. The large-scale functional characterization of genes controlling the pathogenicity of M. oryzae is currently under way, but little is known about heat shock protein 40 (Hsp40) function in the rice blast fungus or any other filamentous plant pathogen. We identified 25 genes encoding putative Hsp40s in the genome of M. oryzae using a bioinformatic approach, which we designated M. oryzae heat shock protein forty (MHF 1-25). To elucidate the roles of these genes, we characterized the functions of MHF16 and MHF21, which encode type ill and type n Hsp40 proteins, respectively. MHF16 and MHF21 expression was not significantly induced by heat shock, but it was down-regulated by cold shock. Knockout mutants of these genes $({\Delta}$mhf16 and ${\Delta}$mhf21) were viable, but conidiation was severely reduced. Moreover, sectoring was observed in the ${\Delta}mhf16$ mutant when it was grown on oatmeal agar medium. Conidial germination, appressorium formation, and pathogenicity in rice were not significantly affected in the mutants. The defects in conidiation and colony morphology were fully complemented by reintroduction of wild type MHF16 and MHF21 alleles, respectively. These data indicate that MHF16 and MHF21 play important roles in conidiation in the rice blast fungus.

Climate Change Impact on Korean Stone Heritage: Research Trends and Prospect (국내 석조유산의 기후변화 영향: 연구동향과 미래전망)

  • Kim, Jiyoung
    • Journal of Conservation Science
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    • v.32 no.3
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    • pp.437-448
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    • 2016
  • Studies on vulnerability of cultural heritage and adaptation strategy to worldwide climate change have been actively carried out in advanced countries since the late 20th century, and this established a valid research methodology and piled up climate and deterioration dataset in the field of climate change. Meanwhile, we still have tasks to acquire related scientific data despite referencing political researches in Korea. Applying Korean future climate to impact analysis, deterioration of Korean stone heritage is likely prospected to change into complexity in terms of physical, chemical and biological weathering that may bring impacts on conservation business and administrative field of cultural heritage. Further studies will ensure detailed implication of climate change impact on Korean stone heritage by means of down-scaling analysis of areas to local scale and dataset frequency to an hour. It is important to sort out capability and vulnerability of the stone heritage to future environment, and to make an adaption and prevention strategies.

Public Telephone Design by Approach Method of Human Sensibility (감성적 접근방법에 의한 공중전화기 디자인에 대한 연구)

  • 홍현주;정석길;이상도
    • Archives of design research
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    • v.11 no.3
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    • pp.35-44
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    • 1998
  • Acording to the trend of highly increasing the public telephone, understanding consumer's design sensibility is based on designing consumer products reflecting user's identity and it comes out as a result of wore competitive products. For this study, consumer's desirous sensibility of the public telephone has been measured in checkable numbers. First of all, 35 sensible words are narrowed down from 168 whole sensible(adjective) words using the SD method. By this result, 7 scale form the SD method is applied to reserch on 10 current distributed products form 30 male and female university students. Element factor analysis is used to group the selected sensible words, duster analysis is used yo dassify them, and finally discriminant analysis is used to predict the relationship between design elements and the sensible words. As a result, words of human sensibility is known to divide into 3 factors; esthetics, functionality and safety, and the correlation of design factor is closely related with the the telephone's body, handset, dial board, dial button, ontrol button, color and material. To sum up, better public telephone design comes out from scientific and systematic consumer's desire analysis not designer's design technique.

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A Study on Furniture Design for Disassembly

  • Han, Jung-Yeob
    • Journal of the Korea Furniture Society
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    • v.18 no.2
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    • pp.91-99
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    • 2007
  • Modernity which is superficial phenomenon set off the mass scale for mass consumption and provide uniformly artificial environment. But natural destruction, environment pollution, resources exhaustion and so on has been caused by this and now ecology is threatened by destruction and damage beyond the limitation and human beings survival is even threatened. Accordingly furniture development for environment preservation considered environment problem is the urgent real situation. Recent paradigm is the concept of Eco-design which is the green design possible to live together in symbiosis, and new types of alternative furniture are needed in Korea as well. 'Furniture for disassembly' is presented as new method for alternative furniture. Furniture for disassembly can be presented by mainly two directions. The first main characteristic is what is assembled by the use of woodworking joints technique as an assembly structure system without any hardware. The second is what is presented as the structure possible to be assembled by simple manual tools with hardware without any glue. The advantages of furniture for disassembly are environment preservation, space application, transportation efficiency and shapeliness. In manufacture method which is different from present furniture, the application of traditional truss technique which uses various types of custom-made and connection technique in case of assemble structure system without hardware is the typical differences. This assembly method expects not only interest induction about assembly and disassembly of diagram per sub materials but also the development of emotion, the improvement of collaboration, space perception ability and shape sense, the improvement of solid body structure insight and so on, when it use in the furniture for children with the application to many kinds of structure with BANGDOOSANJ (Wedged), JUMUGJANGBU (Dovetail) or NABIEUNJANG (Dovetail Keys) and so on.

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A Study on Characteristics Analysis about Pressure Loss Coefficient for Inflator Filter (인플레이터 필터에 대한 압력손실계수 특성해석 연구)

  • Yoo, Il-Hoon;Kim, Byeong-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.12
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    • pp.5696-5703
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    • 2012
  • In this study, the characteristics of filter that make up the automotive airbag system were analyzed. The gas pressure change of airbag is directly impacted by the filter. However, it is uncertain how much the design factors of filter affect the pressure of airbag. And it is difficult to access the pressure loss coefficient in the respect of characteristics of the airbag filter in the simulation method. To solve this problem, this study suggests pressure loss coefficient of the filter using simulation analysis. But it is impossible to interpret a sudden increase of pressure such as airbag filter. To solve this problem, by applying interpolation and scale down method, analysis was processed. Also, through the simulation interpretation of airbag filter's pressure loss coefficient, the guidelines for the filter design could be suggested.

Influence of ventilation rate on the aerodynamic interference between two extra-large indirect dry cooling towers by CFD

  • Ke, S.T.;Liang, J.;Zhao, L.;Ge, Y.J.
    • Wind and Structures
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    • v.20 no.3
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    • pp.449-468
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    • 2015
  • Current wind-resistance designs of large-scale indirect dry cooling towers (IDCTs) exclude an important factor: the influence of the ventilation rate for radiator shutter on wind loads on the outer surfaces of the tower shell. More seemingly overlooked aspects are the effects of various ventilation rates on the wind pressure distribution on the tower surfaces of two IDCTs, and the feature of the flow field around them. In order to investigate the effects of the radiator shutter ventilation rates on the aerodynamic interference between IDCTs, this paper established the numerical wind tunnel model based on the Computational Fluid Dynamic (CFD) technology, and analyzed the influences of various radiator shutter ventilation rates on the aerodynamic loads acting upon a single and two extra-large IDCTs during building, installation, and operation stages. Through the comparison with the results of physical wind tunnel test and different design codes, the results indicated that: the influence of the ventilation rate on the flow field and shape coefficients on the outer surface of a single IDCT is weak, and the curve of mean shape coefficients is close to the reference curve provided by the current design code. In a two-tower combination, the ventilation rate significantly affects the downwind surface of the front tower and the upwind surface of the back tower, and the larger positive pressure shifts down along the upwind surface of the back tower as the ventilation rate increases. The ventilation rate significantly influences the drag force coefficient of the back tower in a two-tower combination, the drag force coefficient increases with the ventilation rate and reaches the maximum in a building status of full ventilation, and the maximum drag coefficient is 11% greater than that with complete closure.

Application of ozone treatment in cooling water systems for energy and chemical conservation

  • Ataei, Abtin;Mirsaeed, Morteza Ghazi;Choi, Jun-Ki;Lashkarboluki, Reza
    • Advances in environmental research
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    • v.4 no.3
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    • pp.155-172
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    • 2015
  • In this study, a complete set of recirculating cooling water system and the required instruments were built in a semi-industrial-scale and a 50 g/h ozone generation plant and a chlorine system were designed for cooling water treatment. Both chlorination and ozonation treatment methods were studied and the results were analyzed during two 45-days periods. The concentrations of ozone and chlorine in recirculating water were constant at 0.1 mg/lit and 0.6 mg/lit, respectively. In ozone treatment, by increasing the concentration cycle to 33%, the total water consumption decreased by 26% while 11.5% higher energy efficiency achieved thanks to a better elimination of bio-films. In case of Carbon Steel, the corrosion rate reached to 0.012 mm/yr and 0.025 mm/yr for the ozonation and chlorination processes, respectively. Furthermore, consumptions of the anti-corrosion and anti-sedimentation materials in the ozone cooling water treatment were reduced about 60% without using any oxidant and non-oxidant biocides. No significant changes in sediment load were seen in ozonation compared to chlorination. The Chemical Oxygen Demand of the blow-down in ozonation method decreased to one-sixth of that in the chlorination method. Moreover, the soluble iron and water turbidity in the ozonation method were reduced by 97.5% and 70%, respectively. Although no anaerobic bacteria were seen in the cooling water at the proper concentration range of ozone and chlorine, the aerobic bacteria in chlorine and ozone treatment methods were 900 and 200 CFU/ml, respectively. The results showed that the payback time for the ozone treatment is about 2.6 years.

Numerical modelling of a pile-supported embankment using variable inertia piles

  • Dia, Daniel;Grippon, Jerome
    • Structural Engineering and Mechanics
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    • v.61 no.2
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    • pp.245-253
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    • 2017
  • The increasing lack of good quality soils allowing the development of roadway, motorway, or railway networks, as well as large scale industrial facilities, necessitates the use of reinforcement techniques. Their aim is the improvement of the global performance of compressible soils, both in terms of settlement reduction and increase of the load bearing capacity. Among the various available techniques, the improvement of soils by incorporating vertical stiff piles appears to be a particularly appropriate solution, since it is easy to implement and does not require any substitution of significant soft soil volumes. The technique consists in driving a group of regularly spaced piles through a soft soil layer down to an underlying competent substratum. The surface load being thus transferred to this substratum by means of those reinforcing piles, which illustrates the case of a piled embankment. The differential settlements at the base of the embankment between the soft soil and the stiff piles lead to an "arching effect" in the embankment due to shearing mechanisms. This effect, which can be accentuated by the use of large pile caps, allows partial load transfer onto the pile, as well as surface settlement reduction, thus ensuring that the surface structure works properly. A technique for producing rigid piles has been developed to achieve in a single operation a rigid circular pile associated with a cone shaped head reversed on the place of a rigid circular pile. This technique has been used with success in a pile-supported road near Bourgoin-Jallieu (France). In this article, a numerical study based on this real case is proposed to highlight the functioning mode of this new technique in the case of industrial slabs.

Video Ranking Model: a Data-Mining Solution with the Understood User Engagement

  • Chen, Yongyu;Chen, Jianxin;Zhou, Liang;Yan, Ying;Huang, Ruochen;Zhang, Wei
    • Journal of Multimedia Information System
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    • v.1 no.1
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    • pp.67-75
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    • 2014
  • Nowadays as video services grow rapidly, it is important for the service providers to provide customized services. Video ranking plays a key role for the service providers to attract the subscribers. In this paper we propose a weekly video ranking mechanism based on the quantified user engagement. The traditional QoE ranking mechanism is relatively subjective and usually is accomplished by grading, while QoS is relatively objective and is accomplished by analyzing the quality metrics. The goal of this paper is to establish a ranking mechanism which combines the both advantages of QoS and QoE according to the third-party data collection platform. We use data mining method to classify and analyze the collected data. In order to apply into the actual situation, we first group the videos and then use the regression tree and the decision tree (CART) to narrow down the number of them to a reasonable scale. After that we introduce the analytic hierarchy process (AHP) model and use Elo rating system to improve the fairness of our system. Questionnaire results verify that the proposed solution not only simplifies the computation but also increases the credibility of the system.

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An Experimental Study on Shield TBM Tunnel Face Stability in Soft Ground (연약지반에서의 쉴드 TBM 굴착시 막장면 안정성 평가를 위한 실험적 연구)

  • Kim, Yong-Man;Lee, Sang-Duk;Choo, Seok-Yeon;Koh, Sung-Yil
    • Journal of the Korean Society for Railway
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    • v.16 no.1
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    • pp.47-51
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    • 2013
  • In this study, we carried out an experimental shield TBM excavation model test using a down-scale device in soft clay, to understand tunnel-face stability properties in relation to changes in slurry pressure. We performed five tests according to tunnel depth (0.5D, 0.75D, 1.0D, 1.25D, 1.5D), and compared theoretical tunnel-face pressure with model test results. The range in theoretical tunnel-face slurry pressure ($P_{min}{\leq}P_{slurry\;pressure}{\leq}P_{max}$), which is determined by earth pressure and water level, was very similar to the model test result. This result was due to the more isotropic condition of the soft clay ground, than of rocky ground.