• Korean Journal of Exercise Nutrition
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• v.25 no.4
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• pp.45-53
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• 2021

[Purpose] In this study, we investigated whether a 70% ethanolic (EtOH) extract of Sargassum horneri had antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated macrophage-like RAW 264.7 cells. [Methods] The proximate composition, fatty acids, amino acids, and dietary fiber of S. horneri, various biologically active compounds, and antioxidant activity were analyzed. [Results] The DPPH and ABTS free radical scavenging activities, as well as the reduction power, of the S. horneri extract used here were significantly increased in a concentration-dependent manner. This indicates that S. horneri contains bioactive compounds, such as phenols and flavonoids, that have excellent antioxidant activity. The cellular viability and metabolic activity results confirmed that the extract had no discernible toxicity at concentrations up to 100 ㎍/mL. The levels of nitrites and cytokines (PGE₂, TNF-α and IL-6), which mediate pro-inflammatory effect, were significantly inhibited by treatment with either 50 or 100 ㎍/mL S. horneri extract, whereas that of IL-1β was significantly inhibited by treatment with 100 ㎍/mL of the extract. Similarly, the expression of iNOS and COX-2 proteins also decreased according to 50 or 100 ㎍/mL extract concentrations. NF-κB binding to DNA was also significantly inhibited by treatment with 100 ㎍/mL of extract. [Conclusion] These results suggest that 70% EtOH extracts of S. horneri can relieve inflammation caused by disease or high intensity exercise.

• ALGAE
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• v.21 no.4
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• pp.463-470
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• 2006

Studies have been made of the species composition and variation of benthic marine algae at subtidal zone of Uljin on the east coast of Korea. Destructive method was employed to measure biomass over four seasons (2000-2002). Of 87 marine algae species identified, 11 were Chlorophyta, 29 were Phaeophyta and 47 were Rhodophyta. Dominant species in biomass were Ulva pertusa, Undaria pinnatifida and Sargassum piluliferum in summer, Laminaria religiosa, Sargassum fulvellum and Gigartina tenella in autumn, Codium fragile, Undaria pinnatifida, Sargassum horneri, S. piluliferum and S. ringgoldianum in winter, and Undaria pinnatifida, Dictyopteris divaricata, Sargassum confusum and S. horneri in spring. In general, green algae (Ulva pertusa, Codium fragile) and brown algae (Undaria pinnatifida, Sargassum fulvellum, S. horneri, S. piluliferum) appeared predominantly in the 3, 6 m depths and red algae (Gelidium amansii, Plocamium telfairiae) in the 9, 12 m depths. The barren ground of the rocky shore might provide the decrease of benthic marine algae biomass and species.

• Ocean and Polar Research
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• v.44 no.1
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• pp.61-67
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• 2022

In this study, in order to evaluate the anti-obesity effect of sargassum horneri extract, the effects of the extract on lipase activity and preadipocyte differentiation in 3T3-L1 cells were investigated. S. horneri extract between 0.0 and 1.0 mg/mL showed no cytotoxicity and inhibited lipase activity by 68.1%. When S. horneri extract was utilized at levels of 0.25, 0.5, and 1.0 mg/mL in 3T3-L1 cells, preadipocytes differentiation decreased by 11.4, 19.7, and 25.6%, respectively, showing anti-obesity effects. In addition, after treatment with 1.0 mg/mL S. horneri extract, the mRNA expression levels of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), and stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 cells were significantly decreased (p < 0.05) by 65.2, 54.9, 50.0, 33.8, and 33.8% respectively. These results showed that S. horneri extract suppresses lipase activity and prophylactic preadipocyte differentiation in 3T3-L1, and thus can be used as an anti-obesity agent in functional foods and medicines.

• ALGAE
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• v.18 no.4
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• pp.365-369
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• 2003

Algal flora and community structure of benthic marine algae in the subtidal zone, Yeongil Bay area were studied. The number of algal species in the quadrats were identified totally 78: 2 Cyanophyta, 10 Chlorophyta, 19 Phaeophyta, and 47 Rhodophyta. The dominant species based on biomass were Sargassum hornerii, Undaria pinnatifida, and Sargassum thunbergii. Vertical distribution was clear by the representative algae such as, Undaria pinnatifida, Sargassum thunbergii, Sargassum horneri, Corallina spp. in the upper subtidal zone, Undaria pinnatifida, Sargassum horneri, Caulerpa okamurae in the middle zone, and Dictyopteris divaricata in the lower zone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.5
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• pp.689-693
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• 2016

Sargassum golden tides occurred in Shinan-gun and Jeju Island, Korea, between January and May 2015. In Shinangun, 5,180 tons of Sargassum were removed from 3,339 ha of coastline, while 20,000 tons were removed from Jeju Island. The huge floating masses of seaweed caused damage to fisheries in Shinan-gun including abalone sea cage, Pyropia and Saccharina farms off the southwest coast of Korea. Drifting Sargassum also washed ashore on Jeju Island. The species of Sargassum in these golden tides was identified as S. horneri (Turner) C. Agardh, based on morphology and molecular data, and it appears to have originated from the East China Sea. This is the first report on Sargassum golden tides causing damage to fisheries in Korea.

• Analytical Science and Technology
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• v.12 no.3
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• pp.196-202
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• 1999

Brown algae, Sargassum horneri, was used as an adsorbent to remove heavy metal Cd(II) and Pb(II) ions. Sargassum horneri picked in East Coast was formulated into the grain with the size of 40-60 mesh after wind dry. Batch method was used in order to investigate the adsorption rate by measuring the adsorption amounts with shaking time. In the column method, the adsorption amounts were measured by flowing metal solutions into the algae-packed column at the rate of 1 ml/min. Adsorption amounts in both batch method and column method were in the following order : pH 10.5>7.0>3.5. It was found that Pb(II) was more adsorbed on the algae grain than Cd(II). It was also revealed that the adsorption amounts reached the maximum within 5 minutes irrespective of pH condition in the batch method. It was concluded that the batch method was more effective than the column method in terms of recovery rate.

• ALGAE
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• v.23 no.4
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• pp.327-334
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• 2008

Seaweed biomass was estimated using a nondestructive sampling method in the rocky intertidal and subtidal zone in Daejin on the mid-east coast of Korea from August 2006 to April 2008. Seasonal sampling were done at the depth of 0, 1, 5, 10 m using 50 x 50 cm quadrat. A total of 95 species, including 11 chlorophytes, 26 phaeophytes, 57 rhodophytes and one sea grass were identified. Mean biomass was comprised of 1,292 g wet wt m$^{-2}$ and the biomass values varied seasonally from 904 g to 1,945 g. Dominant species in biomass were Sargassum spp. (S. horneri, S. yezoense), Corallina pilulifera, Phyllospadix japonica, Symphyocladia latiuscula, Hizikia fusiformis, Codium arabicum and Chondrus ocellatus at Daejin. The vertical distribution of algae were characterized by Corallina pilulifera, Symphyocladia latiuscula, Sargassum spp. (S. confusum, S. yezoense), Hizikia fusiformis and Grateloupia elliptica at intertidal zone, Sargassum spp. (S. fulvellum, S. horneri, S. yezoense), Phyllospadix japonica, Codium arabicum, Undaria pinnatifida and Corallina pilulifera at 1 m depth, Sargassum spp. (S. horneri, S. micracanthum, S. yezoense), Phyllospadix japonica, Grateloupia elliptica, Gelidium amansii and Codium arabicum at 5 m depth and Phyllospadix japonica, Codium arabicum, Sargassum spp. (S. horneri, S. micracanthum, S. yezoense) and Undaria pinnatifida at 10 m depth.

• Journal of Life Science
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• v.28 no.10
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• pp.1179-1185
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• 2018

Recently, Sargassum horneri, the marine weed inhabiting the shoreline, beach, and littoral sea area, has caused serious damage to intensive aquaculture farms particularly those around Jeju Island, South Korea. The purpose of this study was to investigate the diversity of microorganisms in Sargassum horneri and to provide basic data on ecological problems by identifying microbial functions. A total of 88 isolates were identified by 16S rRNA sequencing. Proteobacteria was the dominant phylum accounting for 88%, including class ${\alpha}-proteobacteria$, six genera, and ten species. The dominating genus, Pseudobacter, accounted for 40% in Pseudorhodobacter, 20% in Paracoccus, and the remaining at 10% each were Rhizobium, Albirhodobacter, Skermanella, and Novosphingobium. Class ${\beta}-proteobactera$ included five genera and ten species. Genus Hydrogenophaga accounted for 50%, while genus Azoarcus accounted for 20%, and the remaining Oxalicibacterium, Duganella, and Xenophilus were 10% each. Class ${\gamma}-proteobacteria$ with 13 genera and 57 species, accounted for 74% in phylum Proteobacteria, 23% in Shewanella, 19% in Cobetia, 12% in Pseudomonas, 4% each in Vibrio and Serratia, and 2% each in Rheinheimera, Raoultella, Pantoea, Acinetobacter, Moraxella, and Psychrobacter genera. In addition, Actinobacteria with two species of Nocardioides genera accounted for 50%, and Bacteroidetes accounted for 33%, with three genera and five species that included Lacihabitans and Mariniflexile. The remaining Dyadobacter, Cellulophaga, and Ferruginibacter genera each accounted for 11%.

• ALGAE
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• v.33 no.4
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• pp.341-349
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• 2018

Sargassum horneri is edible brown seaweed abundant along the coasts of Jeju Island, South Korea. In addition to the native S. horneri population, a large amount of S. horneri has been found to invade Jeju Island from the east coast of China. Thus, S. horneri of both Korea (SK) and China (SC) strains now inhabits along with the shore of Jeju Island and have become a threat to the coastal biodiversity. However, they could be used in obtaining functional ingredients for industrial level applications provided an optimized cost effective strategy. In the present study, we compared SK and SC strains for the extraction efficiency, components, antioxidant, and anti-inflammatory properties of 80% methanolic extracts and their partially purified fractions. According to the results, two strains indicated similar bioactive properties such as DPPH and alkyl radical scavenging activity as well as anti-inflammatory activities on lipopolysaccharide-stimulated RAW 264.7 cells. The yield of 80% methanol extract from SC was higher than SK. However, the yields of the ethyl acetate and chloroform fractions from SK were higher than those of SC strain. The major peaks in the high-performance liquid chromatography chromatograms, which was identified as Apo-9 fucoxanthinone, indicated that both methanolic extracts of SK and SC contains major target peaks but with different amounts. This study might be useful for developing functional materials from SC and SK in future.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.6
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• pp.909-917
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• 2020

This study was conducted to evaluate dietary supplementation of Sargassum horneri (SH) meal on growth performance, feed utilization, innate immunity, antioxidant capacity and disease resistance of Pacific white shrimp. The diets were added with graded levels of SH meal by 0, 0.5, 1, 2, 4 and 8% (designated as Con, SH0.5, SH1, SH2, SH4 and SH8, respectively). Quadruplicate groups of shrimp were hand-fed with one of the diets five times daily for 39 days. The innate immunity and antioxidant capacity of shrimp were significantly improved by the dietary SH supplementation. The cumulative survival of shrimp exposed to Vibrio parahaemolyticus in a challenge was higher in shrimp group fed SH diets, except for SH8, than that of shrimp fed the control diet. Growth performance and feed utilization of the shrimp were significantly decreased with the dietary SH inclusions except for 0.5%. Results indicated that dietary SH meal could be used as a functional supplement for improvements in innate immunity, antioxidant capacity and disease resistance in Pacific white shrimp. The suggested dietary inclusion level of dried SH is approximately 0.5% for the shrimp.