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http://dx.doi.org/10.4217/OPR.2022006

Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract  

Hong, Ji Woo (Department of Food Science, Sunmoon University)
Park, Ha Young (Department of Food Science, Sunmoon University)
Park, Jae Hyun (Department of Food Science, Sunmoon University)
Kim, So Hee (Department of Food Science, Sunmoon University)
Kim, Han A (Department of Food Science, Sunmoon University)
Kim, Jin-Woo (Department of Food Science, Sunmoon University)
Publication Information
Ocean and Polar Research / v.44, no.1, 2022 , pp. 61-67 More about this Journal
Abstract
In this study, in order to evaluate the anti-obesity effect of sargassum horneri extract, the effects of the extract on lipase activity and preadipocyte differentiation in 3T3-L1 cells were investigated. S. horneri extract between 0.0 and 1.0 mg/mL showed no cytotoxicity and inhibited lipase activity by 68.1%. When S. horneri extract was utilized at levels of 0.25, 0.5, and 1.0 mg/mL in 3T3-L1 cells, preadipocytes differentiation decreased by 11.4, 19.7, and 25.6%, respectively, showing anti-obesity effects. In addition, after treatment with 1.0 mg/mL S. horneri extract, the mRNA expression levels of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), and stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 cells were significantly decreased (p < 0.05) by 65.2, 54.9, 50.0, 33.8, and 33.8% respectively. These results showed that S. horneri extract suppresses lipase activity and prophylactic preadipocyte differentiation in 3T3-L1, and thus can be used as an anti-obesity agent in functional foods and medicines.
Keywords
sargassum horneri; marine species; adipogenesis; 3T3-L1; anti-obesity;
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